scholarly journals Antagonistic Potentiality of Some Soil Fungi Against Six Fungal Pathogens Isolated From Cotton (Gossypium Hirsutum L.) Seeds

2021 ◽  
Vol 46 (2) ◽  
pp. 143-153
Author(s):  
Amina Khatun ◽  
Shamim Shamsi ◽  
Abul Bashar
Keyword(s):  
Gossypium Hirsutum ◽  
Growth Inhibition ◽  
Radial Growth ◽  
Fungal Pathogens ◽  
Soil Fungi ◽  
The Other ◽  
Dual Culture ◽  
Gossypium Hirsutum L ◽  
Volatile Metabolites ◽  
Other Hand

The antagonistic potentials of four soil filamentous fungi viz., Aspergillus flavus Link, A. fumigatus Fresenius, A. niger van Tieghem and Trichoderma viride Pers. against six pathogens isolated from 14 varieties of cotton (Gossypium hirsutum L.) were evaluated by "dual culture colony interaction”, volatile and non-volatile metabolites. In dual culture colony interaction, out of four soil fungi, T. viride was found be the most effective to control the growth of the cotton pathogens. T. viride showed the highest growth inhibition against Curvularia lunata, Fusarium moniliforme, Mucor sp. and Rhizoctonia solani. On the other hand A. niger showed the highest growth inhibition against Fusarium nivale and A. fumigatus showed the maximum growth inhibition against C. gloeosporioides. The highest inhibition of radial growth of C. lunata, F. nivale and F. moniliforme was observed might be due to the secretion of volatile metabolites of T. viride whereas, the maximum inhibition of radial growth of C. gloeosporioides was observed because of the volatile metabolites of A. flavus. Mucor sp. and R. solani were inhibited due to the volatile metabolites of A. fumigatus. The greatest radial growth inhibition of C. lunata and F. moniliforme were noticed in case of T. viride owing to the effect of non-volatile metabolites. On the other hand, the radial growth of C. gloeosporioides and F. nivale were inhibited highest amount for the effect of non-volatile metabolites of A. niger, whereas highest growth inhibition of Mucor sp. and R. solani was observed due to the non-volatile effect of A. fumigatus. The present investigation suggests that the isolates of Aspergillus and Trichoderma may be further exploited as potential biocontrol agents against the fungal pathogens of cotton in field trial. Asiat. Soc. Bangladesh, Sci. 46(2): 143-153, December 2020

scholarly journals Antagonistic potentials of some soil fungi against three post‐harvest pathogenic fungi of Carica papaya L.

2019 ◽  
Vol 28 (1) ◽  
pp. 1-7
Author(s):  
Rezuana Binte Helal ◽  
Shamim Shamsi
Keyword(s):  
Growth Inhibition ◽  
Radial Growth ◽  
Soil Fungi ◽  
Carica Papaya ◽  
Trichoderma Viride ◽  
Pathogenic Fungi ◽  
Dual Culture ◽  
Volatile Metabolites ◽  
Maximum Inhibition ◽  
Fusarium Sp

Four antagonistic fungi were isolated from the papaya field soil by serial dilution and were identified as Aspergillus flavus Link., A. fumigatus Fresenius, A. niger van Tiegh. and Trichoderma viride Pers. The soil fungi were selected to evaluate their antagonistic potentiality against the three postharvest pathogenic fungi of Carica papaya L. following "dual culture colony interaction” and volatile and non-volatile metabolites. In dual culture colony interaction, out of four soil fungi, T. viride showed the highest growth inhibition on C. gloeosporioides (84.28%), F. nivale (95.23%) and Fusarium sp. (87.15%). On the other hand A. niger showed the highest growth inhibition on C. gloeosporioides (77.39%), F. nivale (98.63%) and Fusarium sp. (35.05%). The maximum inhibition of radial growth of C. gloeosporioides (77.64%), F. nivale (58.76%) and Fusarium sp. (79.37%). were observed in case of T. viride owing to the volatile metabolites. Whereas the maximum inhibition of radial growth of C. gloeosporioides (58.23%), F. nivale (37.43%) and Fusarium sp. (82.31%) were observed in case of A. niger owing to the volatile metabolites. The maximum inhibition of radial growth of C. gloeosporioides (90.90%), F. nivale (89.13%) and Fusarium sp. (76.84%) were observed in case of T. viride owing to the effect of non-volatile metabolites. The maximum inhibition of radial growth of C. gloeosporioides (92.42%), F. nivale (73.01%) and Fusarium sp. (68.67%) were observed in case of A. niger owing to the effect of non-volatile metabolites. Trichoderma viride and A. niger may be exploited commercially as a biocontrol agent against anthracnose and fruit rot pathogens of papaya. Dhaka Univ. J. Biol. Sci. 28(1): 1-7, 2019 (January)

scholarly journals Antagonistic Potentials of Selected Soil Fungi Against Three Pathogenic Fungi of Tagetes Erecta L. and T. Patula L

2020 ◽  
Vol 29 (2) ◽  
pp. 147-153
Author(s):  
Mahfuza Aktar ◽  
Shamim Shamsi
Keyword(s):  
Radial Growth ◽  
Soil Fungi ◽  
Trichoderma Viride ◽  
Pathogenic Fungi ◽  
Complete Inhibition ◽  
Curvularia Lunata ◽  
Dual Culture ◽  
Volatile Metabolites ◽  
Culture Filtrates ◽  
Maximum Inhibition

Three antagonistic fungi were isolated from the field soil of blight infected Tagetes spp. by serial dilution method. The fungi were identified as Aspergillus flavus Link, A. niger van Tiegh. and Trichoderma viride Pers. Antagonistic potentiality of aforesaid fungi were evaluated against the pathogenic fungi of Tageteserecta L. and T. patula L. following "dual culture colony interaction" and volatile and nonvolatile metabolites. The pathogenic fungi were A. alternata, Aspergillus fumigatus and Curvularia lunata. In dual culture colony interaction, out of three soil fungi, T. viride showed the highest growth inhibition on A. alternata (71.03%), A. fumigatus (38.49%) and C. lunata (60.71%). The maximum inhibition of radial growth of A. alternata (74.55%) was observed with the culture filtrates of T. viride owing to volatile metabolites. The maximum inhibition of radial growth of A. fumigatus (37.43%) was observed with the culture filtrates of A. flavus owing to volatile metabolites. The complete inhibition of radial growth of C. lunata was observed with the culture filtrates of A. niger owing to volatile metabolites. The complete inhibition of radial growth of A. alternata was observed with nonvolatile metabolites of A. nigera and T. viride at all concentrations. The complete inhibition of radial growth of A. fumigatus was also observed with nonvolatile metabolites of A. niger at all concentrations. Aspergillus niger and T. viride may be exploited commercially as a biocontrol agent against blight pathogens of T. erecta and T. patula. Dhaka Univ. J. Biol. Sci. 29(2): 147-153, 2020 (July)

scholarly journals In Vitro Antagonism of Trichoderma Verede and Aspergillus Spp. against a Pathogenic Seed Borne Fungus of Sesame

2019 ◽  
Vol 43 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Md Delwar Hosen ◽  
Shamim Shamsi
Keyword(s):  
Aspergillus Flavus ◽  
Soil Fungi ◽  
Trichoderma Viride ◽  
Dual Culture ◽  
Volatile Metabolites ◽  
Inhibiting Effect ◽  
Antagonistic Potential ◽  
Academy Of Sciences ◽  
Growth Inhibiting

Four soil fungi were isolated from the soil by serial dilution and were identified as Aspergillus flavus Link, A. fumigatus Fresenius, A. niger van Tieghem and Trichoderma viride Pers. The soil fungi were selected to evaluate their antagonistic potential against seed borne fungus Fusarium merismoides isolated from sesame. In dual culture colony interaction Trichoderma viride showed the highest (45.88%) growth inhibiting effect on F. merismoides followed by A. niger (40.00%), A. flavus (36.37) and A. fumigatus (30.77%). Volatile metabolites from T. viride showed the highest growth inhibiting effect on F. merismoides (67.69%) and non-volatile metabolites from T. viride showed the highest growth inhibiting effect on F. merismoides (75.00%). Journal of Bangladesh Academy of Sciences, Vol. 43, No. 1, 17-23, 2019

In-vitro bioefficacy of fungal antagonists against root rot of Pea caused by Rhizoctonia solani (Kuhn)

2018 ◽  
Vol 3 (02) ◽  
pp. 166-170
Author(s):  
Safdar Kaiser Hasmi ◽  
R. U. Khan
Keyword(s):  
Growth Inhibition ◽  
Rhizoctonia Solani ◽  
Root Rot ◽  
Radial Growth ◽  
Culture Technique ◽  
Dual Culture ◽  
Time Control ◽  
Trichoderma Koningii ◽  
Petri Plate

In-vitro effectiveness of various antagonistic fungi namely Aspergillus niger, A. flavus, Trichoderma koningii, T. atroviride, and T. harzianum were evaluated against Rhizoctonia solani by dual culture technique on potato dextrose agar. According to the observation recorded after 5 days, all the treatments were found to be superior over control (R. solani), but among all treatments A. niger was found to the most effective antagonist, with highest radial growth inhibition of the pathogen (77.01 percent), followed by A. flavus, T. harzianum and T. koningii i.e., 66.23, 64.42 and 62.20 percent. While as, T. atroviride was found to be the least effective one with minimum growth inhibition i.e., 42.21 percent. Whereas, at the same time control (R. solani) showed 100 percent radial growth and covered the whole Petri Plate within 5 days. All the bio-control agents were significantly effective to inhibit the sclerotia formation and development, except T. atroviride in which formation of sclerotia was recorded, while in all other treatments complete inhibition of sclerotia formation was recorded after 10 days of incubation.

scholarly journals The Induction of High-Frequency Callus with an Elite Cotton (Gossypium Hirsutum L.) from Côte d'Ivoire

2019 ◽  
Vol 8 ◽  
pp. 1104-1117
Author(s):  
Affoué Sylvie Rachelle N'GUESSAN ◽  
Koutouan AYOLIE ◽  
Sopie Edwige Salomé YAPO ◽  
Yao Kouakou Francois KONAN ◽  
Achi Laurent N’CHO ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Callus Induction ◽  
High Frequency ◽  
Culture Medium ◽  
Carbon Sources ◽  
The Other ◽  
High Quality ◽  
Gossypium Hirsutum L ◽  
Hypocotyl Explants ◽  
Cotton Cultivar

In cotton, factors such as carbon sources, hormonal combination, and type of explant influence the responses to callus induction. In this study callus induction was optimized to get high-frequency callus in cotton. Different carbon sources were tested, and among them, glucose at 3% was found to be the best carbohydrate in callus induction. Culture medium supplemented with 2,4-D (0.1 mg.L-1) and KIN (0.5 mg.L-1) gave a high callogenesis response, unlike other hormonal combinations. With explants, hypocotyl (72.93%) appeared easier to induce callus than cotyledon (48.75%) following root (37.5%). So, carbone sources, hormone, and their concentration affect significantly callus induction. The highest frequency of callus induction was then observed when hypocotyl explants were cultured on medium supplemented with 0.1 mg.L-1 of 2,4-D in combination with 0.5 mg.L-1 of KIN and 3% glucose. The induced-callus obtained were friable and of high quality. The other types of combinations resulted in the formation of compact callus with sometimes roots emerging from it. The present study may provide an efficient protocol to improve the callogenesis responses by adjusting the carbohydrates, the hormonal regime, and the type of explant to be cultured for a high-frequency callus induction of the Y331B-R5 cotton cultivar.  

scholarly journals Antagonistic potential of rhizosphere mycoflora against Fusarial wilt of brinjal

2015 ◽  
Vol 24 (2) ◽  
pp. 137-145
Author(s):  
Romana Akter ◽  
KS Hossain ◽  
MA Bashar
Keyword(s):  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Aspergillus Flavus ◽  
Trichoderma Harzianum ◽  
Radial Growth ◽  
Soil Fungi ◽  
Volatile Metabolites ◽  
Penicillium Sp ◽  
Maximum Inhibition ◽  
Antagonistic Potential

Antagonistic potentials of seven rhizoshere soil fungi viz., Aspergillus flavus Link., A. fumigatus Fresen., A. niger Tiegh., A. terreus Thom., Penicillium sp., Trichoderma harzianum Refat. and T. viride Pers. were tested against the Fusarium oxysporum Schlecht. and F. solani (Mart.) Sacc. – the two pathogens, isolated from wilted roots of brinjal plants. Out of seven soil fungi Trichoderma harzianum was found most effective to control the growth of both the test pathogens. More than 50% inhibition of the test pathogens were observed with T. viride, Aspergillus niger and A. fumigatus in colony interaction. The maximum inhibition of radial growth of the test pathogens was observed with volatile metabolites and cell free culture filtrates of Trichoderma harzianum. This fungus may be exploited commercially to control the disease. Dhaka Univ. J. Biol. Sci. 24(2): 137-145, 2015 (July)

Bemisia argentifolii Adult, Nymph and Egg Densities and Egg Distribution on Selected Upland Cottons

2000 ◽  
Vol 35 (1) ◽  
pp. 39-47 ◽  
Author(s):  
C. C. Chu ◽  
T. Freeman ◽  
E. T. Natwick ◽  
J. S. Buckner ◽  
D. R. Nelson ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Upland Cotton ◽  
The Other ◽  
Silverleaf Whitefly ◽  
Bemisia Argentifolii ◽  
Gossypium Hirsutum L ◽  
Egg Distribution ◽  
Leaf Surfaces

Five upland cotton, Gossypium hirsutum L., cultivars, Deltapine (DPL) 50, 5415 and 5432, Fibermax 832 and Siokra L23, were studied in relation to silverleaf whitefly, Bemisia argentifolii Bellows and Perring, oviposition and colonization. Deltapine 5415 and 5432 had the highest numbers of eggs and nymphs and Siokra L-23 the lowest. Siokra L-23 also had the lowest number of adults compared to the other four cultivars. Over 75% of eggs were oviposited on leaf surfaces between veins and 23% were oviposited in veins that were ≤4 cells wide. Few eggs were found on veins that were five or more cells wide. No eggs were inserted into leaf stomata.

scholarly journals In Vitro Evaluation of Bacterial Bioagents and Fungicides Efficiency against Rhizoctonia solani Isolates the Causal agent of Black Scurf Disease of Potato

2021 ◽  
Vol 1 (1) ◽  
pp. 1-11
Keyword(s):  
Rhizoctonia Solani ◽  
Hyphal Growth ◽  
Black Scurf ◽  
The Other ◽  
Dual Culture ◽  
Significant Suppression ◽  
Other Hand ◽  
Time Period ◽  
Bacillis Subtilis

The efficacy of two isolates of bacterial bioagents and two fungicides via, Rhizoctonia solani isolates were evaluated in vitro. In the dual culture assays, significance inhibition ratio of R. solani hyphal growth was obtained in all treatments of Pesudomonas fluoresnces and Bacillis subtilis compared to control. Also, Bacillus subtilis exhibit the highest significant suppression for the mycelial growth of R. solani isolates more than Pseudemonas fluorescens, and the ratios of inhibition differed according to the bioagents and R. solani isolates similar to those results in antifungal activity technique. The volatile metabolite studies revealed that in the first period (three days) inhibition percentage significantly differed according to the tested isolates of R. solani and the applied bioagents, B. subtilis gave the highest inhibition (57.41%) in R. solani isolate 3. Similarly in P. fluoresnces treatments, the highest inhibition was (57.41%) was detected in isolate 3 of R. solani considering that the second tested time period (five day inoculation). B. subtilis showed highest inhibition value on isolate 1, on the other hand P. flouresnce showed high value of inhibition on isolate 2, whereas the lowest inhibition values were produced on isolate 1.Non volatile activity both bacterial bioagents isolates showed different values of inhibition on R. solani isolates. On the other hand Rezolex was most effective against R. solani at two concentrations (0.2 and 0.3), however Topsin-M showed fluctuate inhibition values at two concentration used (0.04% and 0.075%). Further incubation of plates showed suppressed the formation of sclerotia by all the antagonists tested. The results implied that the extent of inhibition by B. subtilis and P. fluoresnces rovides the use of excellent potential antagonists capable of controlling the R. solani in vitro.

Phytotoxicity, Leachability, and Site of Uptake of 2-chloro-2′,6′-diethyl-N-(methoxymethyl) acetanilide

Weed Science ◽  
1969 ◽  
Vol 17 (4) ◽  
pp. 441-444 ◽  
Author(s):  
Y. Eshel
Keyword(s):  
Growth Rate ◽  
Root Growth ◽  
Gossypium Hirsutum ◽  
Root System ◽  
Soil Depth ◽  
The Other ◽  
Slight Reduction ◽  
Culture Solution ◽  
Other Hand ◽  
Different Soils

The toxicity, leachability, and site of uptake of the herbicide 2-chloro-2',6'-diethyl-N-(methoxymethyl) acetanilide (hereinafter referred to as CP 50144) were studied in different soils. Two inches of water leached most of the compound to a soil depth of 2 to 3 inches, when applied at rates of 1 and 2 kg/ha. The inherent toxicity of CP 50144 to cotton (Gossypium hirsutumL., var. Acala 4-42) was studied in culture solution. The herbicide was most toxic to cotton when the entire root system of the crop was exposed to soil treated with CP 50144; reduction of weight of seedlings was accompanied by severe inhibition of root growth. On the other hand, only a slight reduction in growth rate was noticed when only the shoot zone of cotton was exposed to the herbicide.

scholarly journals EFFECT OF COW URINE TREATMENT ON PLANT GROWTH AND ANTI-MICROBIAL ACTIVITY ON GOSSYPIUM HIRSUTUM L.

2021 ◽  
Vol 21 (2) ◽  
Author(s):  
S. Krishnaveni ◽  
M. Mamatha
Keyword(s):  
Antifungal Activity ◽  
Plant Growth ◽  
Gossypium Hirsutum ◽  
Shoot Length ◽  
The Other ◽  
Diffusion Method ◽  
Cow Dung ◽  
Gossypium Hirsutum L ◽  
Cow Urine ◽  
Agar Diffusion Method

In Hindu culture, the cow is treated as a divine animal. Cow's urine, cow dung and Milk are utilized for different purposes. Cow's urine has been utilized as an enhancer of plant growth and an antifungal agent for the current study. The plant selected for this study Gossypium hirsutum L.(cotton).The plant was grown utilizing various concentrations of cow urine, such as 0% (control), 1:10, 1:5, 1:1 for 90 days. Various parameters for example leaf area, shoot length, plant height, root length, as well as biomass of the plant have been monitored. The antifungal activity has been studied by utilizing different concentrations of cow urine against (Alternaria macrospora) using agar diffusion method. Among all the concentrations, the 1:1 concentration showed maximum plant growth compared to other two concentrations. On the other hand, 1:5, 1:10 concentrations showed more antifungal activity. The conclusion of this study found that cow urine can be utilized as a growth enhancer of plant and has antifungal properties.A

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