Comparison of shear-wave velocities obtained with shear-wave elastography of various peripheral lymph nodes in healthy Beagles

Abstract OBJECTIVE To compare shear-wave velocities (SWVs) with shear-wave elastography of various peripheral lymph nodes (LNs). ANIMALS 11 healthy Beagles. PROCEDURES For each dog, bilateral mandibular, medial retropharyngeal, superficial cervical, axillary, superficial inguinal, and popliteal LNs were evaluated with shear-wave elastography in sagittal and transverse scanning planes. Depth of each lymph node was recorded, and intra- and interobserver reliability was determined. RESULTS SWVs for all LNs were significantly higher in the sagittal scanning plane, compared with those in the transverse scanning plane. The SWV of the most superficial LN, the mandibular LN, was significantly higher, compared with that for the other LNs, except for the medial retropharyngeal LN. The SWV of the deepest LN, the medial retropharyngeal LN, was as high as that for the mandibular LN. Intra- and interobserver reliability was excellent. CONCLUSIONS AND CLINICAL RELEVANCE SWVs for normal peripheral LNs of Beagles may serve as a reference to compare with those for other breeds and diseased LNs. Scanning plane, LN depth, and interfering tissues between the LN and the transducer may affect SWV. Shear-wave elastography may not be operator dependent.

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Application of the novel estimation method by shear wave elastography using vibrator to human skeletal muscle

Scientific Reports ◽

10.1038/s41598-020-79215-z ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Wakako Tsuchida ◽

Yoshiki Yamakoshi ◽

Shingo Matsuo ◽

Mayu Asakawa ◽

Keita Sugahara ◽

...

Keyword(s):

Skeletal Muscle ◽

Shear Wave ◽

Human Skeletal Muscle ◽

Estimation Method ◽

Shear Wave Elastography ◽

Muscle Stiffness ◽

Ultrasound Elastography ◽

The Novel ◽

Wave Velocities ◽

Shear Wave Velocities

AbstractIn recent years, non-invasive measurement of tissue stiffness (hardness) using ultrasound elastography has attracted considerable attention. It has been used to evaluate muscle stiffness in the fields of rehabilitation, sports, and orthopedics. However, ultrasonic diagnostic devices with elastography systems are expensive and clinical use of such devices has been limited. In this study, we proposed a novel estimation method for vibration-based shear wave elastography measurement of human skeletal muscle, then determined its reproducibility and reliability. The coefficient of variation and correlation coefficient were used to determine reproducibility and reliability of the method by measuring the shear wave velocities in konjac phantom gels and agar phantom gels, as well as skeletal muscle. The intra-day, day-to-day, and inter-operator reliabilities were good when measuring the shear wave velocities in phantom gels. The intra-day and day-to-day reliabilities were good when measuring the shear wave velocities in skeletal muscle. The findings confirmed adequate reproducibility and reliability of the novel estimation method for vibration-based shear wave elastography. Therefore, the proposed measurement method may be a useful tool for evaluation of muscle stiffness.

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Evaluation of Treatment Effectiveness of Lymphoma with Impairment of Peripherall Lymph Nodes by Ultrasonic Elastography

Journal of oncology: diagnostic radiology and radiotherapy ◽

10.37174/2587-7593-2020-3-4-51-59 ◽

2020 ◽

Vol 3 (4) ◽

pp. 51-59

Author(s):

E. V. Kovaleva ◽

E. A. Gudilina ◽

T. Yu. Danzanova ◽

G. T. Sinyukova ◽

P. I. Lepedatu

Keyword(s):

Lymph Nodes ◽

Shear Wave ◽

Wave Velocity ◽

Shear Wave Velocity ◽

Treatment Effectiveness ◽

Shear Wave Elastography ◽

Ultrasound Elastography ◽

Ultrasound Study ◽

Peripheral Lymph ◽

Compression Ultrasound

Purpose: To evaluate the capabilities of ultrasound elastography in assessing the effectiveness of treatment of patients with special lesions of peripheral lymph nodes in lymphoproliferative lesions.Material and methods: To evaluate the capabilities of ultrasound elastography in assessing the effectiveness of treatment, an ultrasound study was carried out for 93 patients with lesions of peripheral lymph nodes with lymphoma in dynamics before treatment and after 2/3 courses of chemotherapy using Acoustic Force Radiation Impulse (ARFI — shear wave elastography) and eSie Touch (compression elastography).Results: Assessment of the dynamics of the average, minimum and maximum values of the shear wave velocity showed statistically significant differences. The most reliable changes were recorded when assessing the average and maximum indicators of the shear wave velocity (p = 0.0000001). Also, after two / three cycles of chemotherapy, the indicator X, XX m/s (p = 0.00001) was significantly less frequent, which was previously detected in the LN with the most rigid structure. Compression ultrasound elastography revealed softening of the LN structure in the form of an increase in the frequency of occurrence of I and II elastotypes.Conclusions: Our study confirms that ultrasound elastography allows, in a short time and without negative ionizing effects on the patient, to assess the effectiveness of the selected chemotherapy routes.

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Evaluation of the possibilities of shear wave elastography for differentiation of lymphomatous and reactive changes of superficial lymph nodes

Oncohematology ◽

10.17650/1818-8346-2020-15-1-59-64 ◽

2020 ◽

Vol 15 (1) ◽

pp. 59-64

Author(s):

E. V. Kovaleva ◽

T. Yu. Danzanova ◽

G. T. Sinyukova ◽

E. A. Gudilina ◽

P. I. Lepedatu ◽

...

Keyword(s):

Differential Diagnosis ◽

Lymph Nodes ◽

Shear Wave ◽

Wave Velocity ◽

Shear Wave Velocity ◽

Ultrasound Elastography ◽

Wave Velocities ◽

Shear Wave Velocities ◽

Reactive Changes ◽

Hodgkin's Lymphomas

Objective: to evaluate the possibilities of ultrasound elastography for differentiation of reactive and lymphomatous superficial lymph nodes (LN).Materials and methods. The prospective study included 138 patients with enlarged superficial LN. Based on a previous histological examination, patients were divided into two groups: 1st group (n = 108) – patients with non-Hodgkin’s lymphomas and Hodgkin’s lymphoma; 2nd (n = 30) – patients with reactive (inflammatory) changes in superficial LN. All patients underwent ultrasound elastography of the enlarged LN using ARFI technology.Results. According to the results of ultrasound elastography, the average, minimum, and maximum shear wave velocities for enlarged LN in lymphoma (1st group) were 2.616 ± 0.684; 1.980 ± 0.557 and 3.351 ± 0.987 m / s, respectively; for LN with reactive changes (2nd group) – 1.704 ± 0.223; 1.414 ± 0.209 and 2.027 ± 0.261 m / s, respectively. Thus, the average, minimum, and maximum values of shear wave velocities significantly different between the groups (p ˂0.001). The cut off values of the average shear wave velocity in the differential diagnosis of lymphoma and hyperplasia are determined at the level of 2.05 m / s, with a sensitivity of 88.5 %, specificity of 100 %, and AUC of 0.942 (p ˂0.001).Conclusion. Ultrasound elastography demonstrated statistically significant differences in shear wave velocity in the enlarged superficial LN in lymphoma and in inflammatory processes that can be used as a preliminary non-invasive differential diagnosis of enlarged superficial LN in these conditions.

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Comparison of Liver Shear Wave Elastography Measurements using Siemens Acuson S3000, GE LOGIQ E9, Philips EPIQ7 and Toshiba Aplio 500 (Software Versions 5.0 and 6.0) in Healthy Volunteers

Ultraschall in der Medizin - European Journal of Ultrasound ◽

10.1055/a-0651-0542 ◽

2018 ◽

Vol 40 (04) ◽

pp. 504-512 ◽

Cited By ~ 2

Author(s):

Victoria Sofie Gress ◽

Elisa Nadine Glawion ◽

Julian Schmidberger ◽

Wolfgang Kratzer

Keyword(s):

Shear Wave ◽

Shear Wave Elastography ◽

Ultrasound Elastography ◽

Impact Factors ◽

Wave Velocities ◽

Shear Wave Velocities ◽

Right Lobe ◽

The Right ◽

Study Participants ◽

Healthy Liver

Abstract Aim To compare ARFI-based elastography procedures of the GE LOGIQ E9, Philips EPIQ7, and Toshiba Aplio 500 (versions 5.0 and 6.0) ultrasound scanners with the Siemens Acuson S3000 reference scanner in subjects with healthy livers, taking various impact factors into account. Materials and Methods The study consisted of two subsequent study parts. Study 1 (n = 205) evaluated the 2D-SWE techniques of GE and Toshiba 5.0 and the pSWE technique of the Philips scanner against the reference scanner Siemens. Study 2 (n = 113) evaluated Toshiba 6.0 against Siemens in a different cohort. Out of 433 study participants in both studies, 318 (M:F = 122:196) met the inclusion criteria. Elastography measurements were performed in the right lobe of the liver at depths of 3, 5 and 7 cm. At each depth, five measurements were acquired with the Siemens and Philips scanners and one measurement each with the GE and Toshiba machines. Results The Philips (r = 0.58, p < 0.0001) and the GE (r = 0.63, p < 0.0001) scanners showed the strongest correlation of shear wave velocities between Siemens and comparator scanners, at a depth of 5 cm. We found the strongest relationship with Toshiba (software version 6.0) at a depth of 3 cm (r = 0.57, p < 0.0001). Toshiba software version 5.0 did not give satisfactory results. The factors of sex and BMI showed scanner-specific differences in the values measured (p < 0.05). The age of the subjects did not seem to have any effect. Conclusion With the exception of Toshiba software version 5.0, all of the scanners we tested can be recommended without reservation for comparative ultrasound elastography of the healthy liver at measurement depths of 3 cm up to 5 cm.

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Salmonella Persistence within the Peripheral Lymph Nodes of Cattle following Experimental Inoculation

Journal of Food Protection ◽

10.4315/0362-028x.jfp-15-325 ◽

2016 ◽

Vol 79 (6) ◽

pp. 1032-1035 ◽

Cited By ~ 6

Author(s):

T. S. EDRINGTON ◽

G. H. LONERAGAN ◽

K. J. GENOVESE ◽

D. L. HANSON ◽

D. J. NISBET

Keyword(s):

Body Weight ◽

Lymph Node ◽

Lymph Nodes ◽

Enrichment Culture ◽

Lower Leg ◽

Challenge Strain ◽

Peripheral Lymph ◽

Experimental Inoculation ◽

Duration Of Infection

ABSTRACT Utilizing a transdermal method of inoculation developed in our laboratory, the duration of infection of Salmonella in the peripheral lymph nodes of steers was examined. Thirty-six Holstein steers (mean body weight of 137 kg) were inoculated with Salmonella Montevideo (day 0) on each lower leg and both sides of the back and abdomen. Calves were euthanized beginning at 6 h and subsequently on each of days 1, 2, 4, 7, 9, 11, 14, and 21 postinoculation (four animals each time). The subiliac, popliteal, and superficial cervical (prescapular) lymph nodes were collected and cultured (quantitatively and qualitatively) for the challenge strain of Salmonella. The challenge strain was detected via direct culture within the lymph nodes at 6 h postinoculation and on each subsequent necropsy date. Salmonella levels in lymph node were 0.8 to 1.8 log CFU/g. Lymph nodes were generally positive after enrichment culture throughout the experiment. Salmonella elimination appeared to begin approximately 14 days postinoculation. However, elimination was not completed by day 21; therefore, a second experiment was conducted identical to the first except that the time from inoculation to necropsy was extended. Salmonella was recovered via direct culture on each of the necropsy days, and results in general were similar to those of experiment I, except that on days 20, 24, and 28 isolates from serogroups C2 and E1 were identified in addition to the inoculation strain C1 in multiple animals. The data from both experiments indicate that after a single inoculation event, Salmonella would be completely cleared by approximately 28 days. Further research with expanded times between inoculation and necropsy is required for verification.

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