Evaluation of the expression of hexokinase 1, glucokinase, and insulin by canine insulinoma cells maintained in short-term culture

2021 ◽  
Vol 82 (2) ◽  
pp. 110-117
Author(s):  
Orn-usa Suwitheechon ◽  
Thomas Schermerhorn



Head & Neck ◽  
2015 ◽  
Vol 38 (S1) ◽  
pp. E911-E915 ◽  
Author(s):  
Marine Peria ◽  
Jérôme Donnadieu ◽  
Caroline Racz ◽  
Jean-Fortuné Ikoli ◽  
Antoine Galmiche ◽  
...  


1994 ◽  
Vol 5 (3) ◽  
pp. 265-268 ◽  
Author(s):  
A. Younes ◽  
J. Drach ◽  
R. Katz ◽  
D. Jendiroba ◽  
M.H. Sabourian ◽  
...  


1987 ◽  
Vol 93 (4) ◽  
pp. 791-800 ◽  
Author(s):  
A.M.J. Buchan ◽  
D.L. Barber ◽  
M. Gregor ◽  
A.H. Soll


1979 ◽  
Vol 161 (2) ◽  
pp. 153-157 ◽  
Author(s):  
F. Herz ◽  
A. Schermer ◽  
L. G. Koss


Genetika ◽  
2016 ◽  
Vol 48 (3) ◽  
pp. 955-961
Author(s):  
Bojana Petrovic ◽  
Jelena Dukanac-Stamenkovic

Finding of fetal chromosomal mosaicism complicates genetic counseling, as well as pregnancy management. The aim of this study was to determine the risk of confined placental mosaicism in short term culture of chorionic villous samples. We conducted a retrospective review of karyotype analysis results obtained after chorionic villous sampling (CVS) in two years period. A 420 samples of chorionic villi were taken transabdominally and obtained by a semidirect method (overnight incubating culture). All fetuses with CVS mosaicism were under the intensive perinatal care. In all cases of chromosome mosaicism the additional karyotyping was performed from fetal blood samples after 22nd gestational week in order to exclude true fetal mosaicism. After delivery newborns were examined by experienced pediatrician. From 420 analyzed samples in 11 (2,6%) cases we found placental mosaicism. No anomalies were seen in genetic sonogram of this fetuses and mosaicism was confirmed only in one case. Confined placental mosaicism (CPM) was found in 2,1% (9/420) of all analyzed cases, and it made 90% of all placental mosaicism. In 60% (6/10) of placental mosaicism cases we found mosaicism with single aberrant cell. Trisomy 21 mosaicism was the most frequent aberration found in 30% of cases. Finding of mosaicism in chorionic villi sample is at special importance for genetic counseling, because every case has to be reveled individually regarding the type and level of mosaicism. Anyway, in every case of placental mosaicism intensive antenatal monitoring is necessary, with additional chromosome analysis from different tissue in consideration of previous findings.



2019 ◽  
Vol 21 (2) ◽  
pp. 279-292 ◽  
Author(s):  
A. V. Shabaldin ◽  
S. A. Shmulevich ◽  
G. N. Chistyakova ◽  
I. I. Remizova ◽  
E. B. Lukoyanycheva ◽  
...  

We have studied HLA allogeneic interactions in short-term cultures of lymphocytes from the parents having children with congenital heart defects (CHD), or subject to early reproductive losses. Twentyone married couples (CHD as the main group) who had children with sporadic CHD (interventricular septal defect) without chromosomal diseases were observed. Fifty married couples (a comparison group) had two or more reproductive losses in early gestation (up to 9 weeks), denoted as PNPs (miscarriages, missed abortions, habitual miscarriages. Forty-one families with three or more healthy children represented a control group. Immune response in cell cultures was evaluated by increasing expression of HLA-DR in a mixed culture, as compared to spontaneous lymphocyte cultures. Initial labeling of female and male lymphocytes with monoclonal antibodies to CD45 conjugated to different fluorescent dyes (PC-5 and PC-7) allowed us to evaluate the immune response of female lymphocytes to males and vice versa. The suppressor effect of autologous female serum upon the mixed culture of the lymphocytes of the spouses was also evaluated. Results of the present study showed a difference in HLA allogeneic interactions in the short-term culture of lymphocytes registered for spouses with reproductive losses and children with congenital heart defects. Reproductive losses were associated with a low blocking effect of female auto-serum upon allogeneic HLA interactions in the short-term culture of the lymphocytes of the spouses. Congenital heart defects were associated with high activity of female B-lymphocytes (CD3-/HL-DR+) in short-term mixed culture of lymphocytes from the spouses.



Development ◽  
1966 ◽  
Vol 15 (2) ◽  
pp. 213-221
Author(s):  
J. D. Feldman ◽  
D. L. Gardner

The investigations which are described here were originally undertaken in order to investigate the pathogenesis of early hypertension at a time when no morphological changes were evident in adrenergic arteries on light microscopy. Evidence of functional change was therefore sought by comparing the growth patterns of cells from hypertensive and normal mesenteric arteries in culture. The study of the cells which migrated from the explants form the basis for this paper. Month-old Wistar laboratory-strain albino rats were used for the experiment. Young rats were deliberately selected, as fat could be more cleanly dissected away from vessel walls. Also: ‘Tissues from old animals are often more difficult to grow than tissues from young animals and exhibit a longer lag period before growth commences’ (Paul, 1965a). Each week, over a period of 14 weeks, hypertension was induced in a group of rats according to the method of Loomis (1946).



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