Retrospective investigation of automated hematology analyzer–determined indicators of neutrophil activation in blood samples from horses with asthma

AbstractObjectivesWe performed a verification study of the Sysmex XN-3100 hematology analyzer in comparison with the XE-2100 according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI) and the International Council for Standardization in Hematology (ICSH).Materials and methodsBlood samples and quality control materials were used for precision. For comparison, we used the current XE-2100 as the comparative method and analyzed 540 blood samples. The Passing-Bablok and Bland-Altman tests were performed according to the CLSI EP09-A3 and a carryover study was performed according to the CLSI H26-A2 guidelines. The flagging performance of the two analyzers was compared, using two experienced laboratory technicians as the reference method.ResultsThe Sysmex XN-3100 demonstrated high levels of precision for most parameters. For the comparison analysis, all parameters, except for MCHC, monocytes and basophils were within the systematic error limits of desirable biological variability criterion (SeDBV). The carryover was less than 0.4% for all parameters. The flagging performance of the XN-3100 was satisfactory and the overall efficiency was high.ConclusionsThe XN-3100 not only showed a strong correlation and agreement with the XE-2100 but also displayed a comparable analytical sensitivity, and increased specificity, which may result in an improved turnaround time and throughpu.

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Automated detection of malaria: a comparison with microscopy

Annals of King Edward Medical University ◽

10.21649/akemu.v11i3.998 ◽

2016 ◽

Vol 11 (3) ◽

Author(s):

Soad Fadlalla Ali ◽

Sajid Jamil ◽

Muhammad Naveed Akhtar ◽

Muhammad Farooq

Keyword(s):

Laser Light ◽

Automated Detection ◽

Malaria Parasites ◽

Blood Samples ◽

Three Samples ◽

Hematology Analyzer ◽

Analyzer Cell ◽

Scatter Plots ◽

Automated Hematology Analyzer ◽

Light Depolarization

Objective: To compare the automated detection of malaria with microscopy. Methods: In this study 250 blood samples submitted for malaria investigation were studied microscopically for malaria parasites. All samples were additionally analyzed for same parameters with automated hematology analyzer, Cell Dyn 3700 (CD3700). The results from the instrument generated as scatter plots (derived by laser light depolarization), were compared with microscopy results. Results: The atypical depolarizing events or positive patterns were observed in 43 out of 250 samples on cell Dyn 3700, while microscopically 3 7 samples were found to b e positive. Three samples positive on microscopy, were found t o b e negative on CD-3700. Compared with microscopy the sensitivity of CD3700 was 92.5% and specificity was 97.3%. Maximum parasitaemia was 6.5%. Conclusions: Automated detection of malaria by CD3700 automated hematology analyzer is feasible for screening purpose in malaria endemic and non-endemic areas.

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Validation of the Celltac alpha automated hematology analyzer for canine and feline blood samples

Veterinary Clinical Pathology ◽

10.1111/vcp.12019 ◽

2012 ◽

Vol 42 (1) ◽

pp. 11-18 ◽

Cited By ~ 2

Author(s):

Barbara Jane McDaniel ◽

Johannes Hirschberger ◽

Karin Weber

Keyword(s):

Blood Samples ◽

Hematology Analyzer ◽

Automated Hematology Analyzer

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Do Hemoglobin Concentration and Anemia Prevalence Differ Between Capillary and Venous Blood and Between Analysis Methods?

Current Developments in Nutrition ◽

10.1093/cdn/nzaa053_127 ◽

2020 ◽

Vol 4 (Supplement_2) ◽

pp. 922-922

Author(s):

Amanda Wendt ◽

Jillian Waid ◽

Anna Müller-Hauser ◽

Nicholas Kyei ◽

Shafinaz Sobhan ◽

...

Keyword(s):

Pregnant Women ◽

Venous Blood ◽

Hemoglobin Concentration ◽

Mean Difference ◽

Blood Collection ◽

Blood Samples ◽

Analysis Methods ◽

Prevalence Estimates ◽

Hematology Analyzer ◽

Automated Hematology Analyzer

Abstract Objectives Our objective was to compare hemoglobin concentration and anemia prevalence between (1) analysis methods, i.e., the portable HemoCue 201 + and the Sysmex XP-100 automated hematology analyzer, and (2) blood matrix, i.e., venous and capillary, in women and young children. Methods We collected capillary and venous blood samples from 349 non-pregnant women (NPW), 45 pregnant women (PW), and 167 children aged 6–36 months in rural Sylhet Division, Bangladesh in late 2019. We measured hemoglobin concentration in capillary and venous blood using HemoCue 201 + at the point of blood collection in the villages. In addition, hemoglobin concentration was measured in venous EDTA blood samples in the lab using the XP-100 Sysmex automated hematology analyzer within 6 hours of collection. Hemoglobin values were compared using paired t-tests, while anemia prevalence estimates were compared using McNemar tests. Results Venous hemoglobin concentrations were similar (mean difference: 0.3 g/L) when measured by HemoCue and the hematology analyzer. However, among NPW, there was strong evidence that anemia prevalence was higher when measured by HemoCue compared to the hematology analyzer, with similar trends in PW and children. Mean hemoglobin concentrations in capillary blood were lower overall (mean difference: 5 g/L; P ≤ 0.001) and in all subgroups (NPW, PW, and children) compared to venous blood. Anemia prevalence was higher in each population group using capillary (NPW: 37%; PW: 51%; children: 21%) compared to venous measures (NPW: 23%; PW: 36%; children: 10%). Conclusions Across all groups, capillary measures resulted in significantly lower hemoglobin concentrations and higher anemia prevalence estimates, thus likely overestimating anemia in the population. Venous blood samples measured by the two analytic methods were similar. This may point to a biological difference between capillary and venous blood. Funding Sources Data collection was primarily supported by the German Ministry of Education and Research (BMBF). The first author received support from the Alexander von Humboldt Foundation and a Thrasher Research Fund Early Career Award.

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