scholarly journals Evaluation of the performance of sysmex XN-3100 automated hematology analyzer regarding the sysmex XE-2100 and microscopic examination

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Said Incir ◽  
Kerim Erhan Palaoglu
Keyword(s):  
Comparative Method ◽  
Reference Method ◽  
Turnaround Time ◽  
Analytical Sensitivity ◽  
Biological Variability ◽  
Comparison Analysis ◽  
Blood Samples ◽  
Hematology Analyzer ◽  
Quality Control Materials ◽  
Automated Hematology Analyzer

AbstractObjectivesWe performed a verification study of the Sysmex XN-3100 hematology analyzer in comparison with the XE-2100 according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI) and the International Council for Standardization in Hematology (ICSH).Materials and methodsBlood samples and quality control materials were used for precision. For comparison, we used the current XE-2100 as the comparative method and analyzed 540 blood samples. The Passing-Bablok and Bland-Altman tests were performed according to the CLSI EP09-A3 and a carryover study was performed according to the CLSI H26-A2 guidelines. The flagging performance of the two analyzers was compared, using two experienced laboratory technicians as the reference method.ResultsThe Sysmex XN-3100 demonstrated high levels of precision for most parameters. For the comparison analysis, all parameters, except for MCHC, monocytes and basophils were within the systematic error limits of desirable biological variability criterion (SeDBV). The carryover was less than 0.4% for all parameters. The flagging performance of the XN-3100 was satisfactory and the overall efficiency was high.ConclusionsThe XN-3100 not only showed a strong correlation and agreement with the XE-2100 but also displayed a comparable analytical sensitivity, and increased specificity, which may result in an improved turnaround time and throughpu.

scholarly journals Automated detection of malaria: a comparison with microscopy

2016 ◽  
Vol 11 (3) ◽  
Author(s):  
Soad Fadlalla Ali ◽  
Sajid Jamil ◽  
Muhammad Naveed Akhtar ◽  
Muhammad Farooq
Keyword(s):  
Laser Light ◽  
Automated Detection ◽  
Malaria Parasites ◽  
Blood Samples ◽  
Three Samples ◽  
Hematology Analyzer ◽  
Analyzer Cell ◽  
Scatter Plots ◽  
Automated Hematology Analyzer ◽  
Light Depolarization

Objective: To compare the automated detection of malaria with microscopy. Methods: In this study 250 blood samples submitted for malaria investigation were studied microscopically for malaria parasites. All samples were additionally analyzed for same parameters with automated hematology analyzer, Cell Dyn 3700 (CD3700). The results from the instrument generated as scatter plots (derived by laser light depolarization), were compared with microscopy results. Results: The atypical depolarizing events or positive patterns were observed in 43 out of 250 samples on cell Dyn 3700, while microscopically 3 7 samples were found to b e positive. Three samples positive on microscopy, were found t o b e negative on CD-3700. Compared with microscopy the sensitivity of CD3700 was 92.5% and specificity was 97.3%. Maximum parasitaemia was 6.5%. Conclusions: Automated detection of malaria by CD3700 automated hematology analyzer is feasible for screening purpose in malaria endemic and non-endemic areas.

Validation of the Celltac alpha automated hematology analyzer for canine and feline blood samples

2012 ◽  
Vol 42 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Barbara Jane McDaniel ◽  
Johannes Hirschberger ◽  
Karin Weber
Keyword(s):  
Blood Samples ◽  
Hematology Analyzer ◽  
Automated Hematology Analyzer

scholarly journals Do Hemoglobin Concentration and Anemia Prevalence Differ Between Capillary and Venous Blood and Between Analysis Methods?

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 922-922
Author(s):  
Amanda Wendt ◽  
Jillian Waid ◽  
Anna Müller-Hauser ◽  
Nicholas Kyei ◽  
Shafinaz Sobhan ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Venous Blood ◽  
Hemoglobin Concentration ◽  
Mean Difference ◽  
Blood Collection ◽  
Blood Samples ◽  
Analysis Methods ◽  
Prevalence Estimates ◽  
Hematology Analyzer ◽  
Automated Hematology Analyzer

Abstract Objectives Our objective was to compare hemoglobin concentration and anemia prevalence between (1) analysis methods, i.e., the portable HemoCue 201 + and the Sysmex XP-100 automated hematology analyzer, and (2) blood matrix, i.e., venous and capillary, in women and young children. Methods We collected capillary and venous blood samples from 349 non-pregnant women (NPW), 45 pregnant women (PW), and 167 children aged 6–36 months in rural Sylhet Division, Bangladesh in late 2019. We measured hemoglobin concentration in capillary and venous blood using HemoCue 201 + at the point of blood collection in the villages. In addition, hemoglobin concentration was measured in venous EDTA blood samples in the lab using the XP-100 Sysmex automated hematology analyzer within 6 hours of collection. Hemoglobin values were compared using paired t-tests, while anemia prevalence estimates were compared using McNemar tests. Results Venous hemoglobin concentrations were similar (mean difference: 0.3 g/L) when measured by HemoCue and the hematology analyzer. However, among NPW, there was strong evidence that anemia prevalence was higher when measured by HemoCue compared to the hematology analyzer, with similar trends in PW and children. Mean hemoglobin concentrations in capillary blood were lower overall (mean difference: 5 g/L; P ≤ 0.001) and in all subgroups (NPW, PW, and children) compared to venous blood. Anemia prevalence was higher in each population group using capillary (NPW: 37%; PW: 51%; children: 21%) compared to venous measures (NPW: 23%; PW: 36%; children: 10%). Conclusions Across all groups, capillary measures resulted in significantly lower hemoglobin concentrations and higher anemia prevalence estimates, thus likely overestimating anemia in the population. Venous blood samples measured by the two analytic methods were similar. This may point to a biological difference between capillary and venous blood. Funding Sources Data collection was primarily supported by the German Ministry of Education and Research (BMBF). The first author received support from the Alexander von Humboldt Foundation and a Thrasher Research Fund Early Career Award.

scholarly journals Validation of Platelet Counting Accuracy With the Celltac F Automated Hematology Analyzer

2005 ◽  
Vol 2005 (4) ◽  
pp. 235-239 ◽  
Author(s):  
Yutaka Nagai ◽  
Hiroshi Kondo ◽  
Noriyuki Tatsumi
Keyword(s):  
Platelet Count ◽  
Clinical Laboratory ◽  
Ethylenediaminetetraacetic Acid ◽  
Reference Method ◽  
Becton Dickinson ◽  
Accurate Analysis ◽  
Visual Method ◽  
Hematology Analyzer ◽  
Reagent Consumption ◽  
Automated Hematology Analyzer

Rapid and accurate analysis of platelet count plays an important role in evaluating hemorrhagic status. Therefore, we evaluated platelet counting performance of a hematology analyzer, Celltac F (MEK-8222, Nihon Kohden Corporation, Tokyo, Japan), that features easy use with low reagent consumption and high throughput while occupying minimal space in the clinical laboratory. All blood samples were anticoagulated with dipotassium ethylenediaminetetraacetic acid (EDTA-2K). The samples were stored at room temperature (18∘C–22∘C) and tested within 4 hours of phlebotomy. We evaluated the counting ability of the Celltac F hematology analyzer by comparing it with the platelet counts obtained by the flow cytometry method that ISLH and ICSH recommended, and also the manual visual method by Unopette (Becton Dickinson Vacutainer Systems). The ICSH/ISLH reference method is based on the fact that platelets can be stained with monoclonal antibodies to CD41 and/or CD61. The dilution ratio was optimized after the precision, coincidence events, and debris counts were confirmed by the reference method. Good correlation of platelet count between the Celltac F and the ICSH/ISLH reference method (r=0.99), and the manual visual method (r=0.93) were obtained. The regressions werey=0.90x+9.0andy=1.11x+8.4, respectively. We conclude that the Celltac F hematology analyzer for platelet counting was well suited to the ICSH/ISLH reference method for rapidness and reliability.

Effect of Concentration of Trisodium Citrate Anticoagulant on Calculation of the International Normalised Ratio and the International Sensitivity Index of Thromboplastin

1994 ◽  
Vol 72 (01) ◽  
pp. 084-088 ◽  
Author(s):  
E M Duncan ◽  
C R Casey ◽  
B M Duncan ◽  
J V Lloyd
Keyword(s):  
Reference Material ◽  
Oral Anticoagulants ◽  
Reference Method ◽  
Trisodium Citrate ◽  
Sensitivity Index ◽  
Blood Samples ◽  
Orthogonal Regression ◽  
Significant Difference ◽  
International Normalised Ratio ◽  
International Sensitivity Index

SummaryThe aim of this study was to determine whether the concentration of trisodium citrate used to anticoagulate blood has an effect on the INR of the sample and the ISI of the thromboplastin. Five thromboplastins including and Australian reference material were used to measure the prothrombin time of normal and patient samples collected into two concentrations of trisodium citrate - 109 mM and 129 mM. There was no effect of citrate concentration on the INRs determined with the reference material. However for the other four thromboplastins there was a significant difference between INRs for the two citrate groups. The prothrombin times of the samples collected into 129 mM were longer than those collected into 109 mM. This difference was only slight in normal plasma but more marked in patients receiving oral anticoagulants, causing the INRs for patient plasmas collected into 129 mM citrate to be higher then the corresponding samples collected into 109 mM citrate.From orthogonal regression of log prothrombin times by the reference method against each thromboplastin, we found that the ISI for each thromboplastin was approximately 10% lower when determined with samples collected into 129 mM citrate than with samples collected into 109 mM. These results suggest that the concentration of trisodium citrate used for collection of blood samples can affect the calculation of the INR and the calibration of the ISI of thromboplastin. This was found both for commercial thromboplastins prepared by tissue extraction and for a recombinant tissue factor.

Detecting Human CD34 + and CD34 - Hematopoietic Stem and Progenitor Cells Using a Sysmex Automated Hematology Analyzer

2004 ◽  
Vol 10 (4) ◽  
pp. 200-205 ◽  
Author(s):  
F. S. WANG ◽  
R. M. ROWAN ◽  
M. CREER ◽  
A. HAY ◽  
M. DORFNER ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Hematopoietic Stem ◽  
Human Cd34 ◽  
Hematology Analyzer ◽  
Stem And Progenitor Cells ◽  
Automated Hematology Analyzer

scholarly journals Analytical validation of a real-time hydrolysis probe PCR assay for quantifying Plasmodium falciparum parasites in experimentally infected human adults

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Claire Y. T. Wang ◽  
Emma L. Ballard ◽  
Zuleima Pava ◽  
Louise Marquart ◽  
Jane Gaydon ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Plasmodium Falciparum ◽  
18S Rrna ◽  
Drug Efficacy ◽  
18S Rrna Gene ◽  
Molecular Techniques ◽  
Qpcr Assay ◽  
Rrna Gene ◽  
Analytical Sensitivity ◽  
Blood Samples

Abstract Background Volunteer infection studies have become a standard model for evaluating drug efficacy against Plasmodium infections. Molecular techniques such as qPCR are used in these studies due to their ability to provide robust and accurate estimates of parasitaemia at increased sensitivity compared to microscopy. The validity and reliability of assays need to be ensured when used to evaluate the efficacy of candidate drugs in clinical trials. Methods A previously described 18S rRNA gene qPCR assay for quantifying Plasmodium falciparum in blood samples was evaluated. Assay performance characteristics including analytical sensitivity, reportable range, precision, accuracy and specificity were assessed using experimental data and data compiled from phase 1 volunteer infection studies conducted between 2013 and 2019. Guidelines for validation of laboratory-developed molecular assays were followed. Results The reportable range was 1.50 to 6.50 log10 parasites/mL with a limit of detection of 2.045 log10 parasites/mL of whole blood based on a parasite diluted standard series over this range. The assay was highly reproducible with minimal intra-assay (SD = 0.456 quantification cycle (Cq) units [0.137 log10 parasites/mL] over 21 replicates) and inter-assay (SD = 0.604 Cq units [0.182 log10 parasites/mL] over 786 qPCR runs) variability. Through an external quality assurance program, the QIMR assay was shown to generate accurate results (quantitative bias + 0.019 log10 parasites/mL against nominal values). Specificity was 100% after assessing 164 parasite-free human blood samples. Conclusions The 18S rRNA gene qPCR assay is specific and highly reproducible and can provide reliable and accurate parasite quantification. The assay is considered fit for use in evaluating drug efficacy in malaria clinical trials.

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