Evaluation of five commercially available assays and measurement of serum total protein concentration via refractometry for the diagnosis of failure of passive transfer of immunity in foals

2006 ◽  
Vol 228 (2) ◽  
pp. 215-215
Keyword(s):  
Total Protein ◽  
Protein Concentration ◽  
Passive Transfer ◽  
Total Protein Concentration ◽  
Serum Total Protein

scholarly journals Prevalence of failure of passive transfer of immunoglobulins in Holstein calves in Northern Greece and association with management practices

2017 ◽  
Vol 64 (3) ◽  
pp. 193 ◽  
Author(s):  
N. PANOUSIS (Ν. ΠΑΝΟΥΣΗΣ) ◽  
M. KRITSEPI- KONSTANTINOU (Μ. ΚΡΙΤΣΕΠΗ-ΚΩΝΣΤΑΝΤΙΝΟΥ) ◽  
E. KALAITZAKIS (Ε. ΚΑΛΑΪΤΖΑΚΗΣ) ◽  
N. GIADINIS (Ν. ΓΙΑΔΙΝΗΣ) ◽  
G. E. VALERGAKIS (Γ.Ε. ΒΑΛΕΡΓΑΚΗΣ)
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
Protein Concentration ◽  
Management Practices ◽  
Passive Transfer ◽  
Total Protein Concentration ◽  
Northern Greece ◽  
Serum Total Protein ◽  
Herd Level ◽  
Holstein Calves

Objectives of the present study were to estimate prevalence of failure of passive transfer of immunoglobulins in dairy calves in Northern Greece and to investigate factors potentially associated with it. Four hundred and thirty seven clinically healthy calves in 30 farms were included in the study. Age of calves was 18 h to 7 d. Animals were blood sampled and serum total protein concentrations were measured by a refractometer. Two thresholds of total protein concentration were used: 5.2 or 5.5 g dL 1. At calf level, an animal was considered to have failure of passive transfer of immunoblobulins when total protein concentrations were lower than the above thresholds. At herd level, a herd was considered to have failure of passive transfer of immunoglobulins when >20% of sampled calves had total protein concentration was <5.2 or <5.5 g dL-1.Moreover, data on health management on the farm were collected in a purpose-built questionnaire. At 5.2 g dL-1, 20% of the calves and 40% of the herds were considered to have failure of passive transfer of immunoglobulins; when the 5.5 g dL-1 threshold was used, respective prevalences were 26% and 53%. At herd level, mean blood serum total protein concentration tended to be positively affected by a short interval between birth and first colostrum meal, by maintenance of a stock of frozen colostrum and by establishment of a close-up group of dry cows. At calf level, the same factors had a statistically  significant positive effect on blood serum total protein concentration. Moreover, quantity of colostrum received by calves and colostrum condition were also positively related with blood serum total protein concentration. In conclusion, failure of passive transfer of immunoglobulins is a common problem in Holstein calves in Northern Greece. Increased prevalence of the problem implies that increased efforts and management practices need to be applied to ensure the adequate transferof maternal immunoglobulins to newborn calves. Also, it becomes obvious from all the above findings that many farmersare not well informed for management practices that have to implement to ensure adequate amounts of immunoglobulins tonewborn calves. Hence, dissemination of knowledge concerning best management practices for achieving adequate passiveimmunity is considered to be of significant importance.

Evaluation of serum total protein concentration and protein fractions in sheep naturally infected with Babesia ovis

2012 ◽  
Vol 23 (1) ◽  
pp. 151-155 ◽  
Author(s):  
B. Esmaeilnejad ◽  
M. Tavassoli ◽  
S. Asri-Rezaei ◽  
B. Dalir-Naghadeh ◽  
S. H. Pourseyed
Keyword(s):  
Total Protein ◽  
Protein Concentration ◽  
Protein Fractions ◽  
Total Protein Concentration ◽  
Serum Total Protein ◽  
Babesia Ovis

Covariance structures of fat and protein influence the estimation of IgG in bovine colostrum

2016 ◽  
Vol 83 (1) ◽  
pp. 58-66 ◽  
Author(s):  
Mette Marie Løkke ◽  
Rikke Engelbrecht ◽  
Lars Wiking
Keyword(s):  
Specific Gravity ◽  
Total Protein ◽  
Protein Concentration ◽  
Passive Transfer ◽  
Covariance Structures ◽  
Total Protein Concentration ◽  
Variation Pattern ◽  
Broad Variation ◽  
On Farm ◽  
Better Than

On-farm instruments for assessing colostrum quality are needed in order to ensure that the calf is supplied with enough IgG to avoid failure of passive transfer. The aim of this study was to evaluate methods for estimating the IgG concentration in cows' colostrum. This research included 126 colostrum samples from 21 Danish farms with different breeds, ensuring a broad variation pattern in IgG, total protein and fat concentration. Approximately one third of the samples did not fulfil the recommendation of >50 g IgG/l colostrum, and the IgG concentration decreased with time from calving to milking. The ratio of IgG to total protein varied from 6 to 61%, however IgG and total protein were correlated with r2 = 0·70. The variation in fat was independent of variations in protein and IgG. The IgG concentration was measured by ELISA and compared to fast measurements by specific gravity by colostrometer, Brix by refractometer and prediction from infrared spectroscopy. The three fast methods were all correlated to the total protein concentration of colostrum; however specific gravity was also influenced by the fat concentration. Furthermore, specific gravity generally overestimated the IgG concentration, and the cut-off level should be raised to 1050 in order to ensure adequate IgG in colostrum. None of the methods estimated IgG concentration better than the correlation of total protein and IgG, meaning that they all depended on the indirect correlation between total protein and IgG. The results suggest that using a refractometer for quality control of colostrum is an easy and feasible method, and a cut-off level of Brix 22 seems sufficient to assure adequate IgG concentration in colostrum fed to the calf.

Effect of serum total protein concentration on early-life health and growth of dairy calves

2020 ◽  
Vol 257 (1) ◽  
pp. 80-86
Author(s):  
Victor S. Cortese ◽  
Mark A. Kirkpatrick ◽  
Thomas H. Short ◽  
Brian Voortman
Keyword(s):  
Total Protein ◽  
Early Life ◽  
Protein Concentration ◽  
Dairy Calves ◽  
Total Protein Concentration ◽  
Serum Total Protein

Determination of Total Protein Concentration in Solution Using Gold Electrode Modified with Silver Nanoparticles

2014 ◽  
Vol 27 (1) ◽  
pp. 253-257 ◽  
Author(s):  
Patrick Marcel Seumo Tchekwagep ◽  
Charles Péguy Nanseu-Njiki ◽  
Emmanuel Ngameni ◽  
Ravi Danielsson ◽  
Thomas Arnebrant ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Total Protein ◽  
Gold Electrode ◽  
Protein Concentration ◽  
Total Protein Concentration
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