scholarly journals Physiological and perceptual responses to three consecutive official matches in female boxer. A case study

2014 ◽  
Vol 6 (1) ◽  
Author(s):  
Zbigniew Obmiński ◽  
Dariusz Turowski
Keyword(s):  
Creatine Kinase ◽  
Time Profile ◽  
Serum Creatine Kinase ◽  
Capillary Blood ◽  
Short Term ◽  
Blood Indices ◽  
Physiological Cost ◽  
Capillary Blood Sampling

Summary Study aim: The purpose of this study was to assess the physiological cost of three consecutive official boxing fights played during a 3-day tournament and two non-contact specific drills against handheld pads of the same time-profile as the contest, 4 × 2 minutes with 1-minute intervals between them. This assessment was based on the determination of selected hormones and metabolites in the blood sampled directly prior to the contests and throughout short-term post-contest recovery. Material and methods: A female amateur boxer was enrolled on the study during a 3-day Polish Boxing Championship, where one match was played on each day. The timing of capillary blood sampling during each match and the drill was as follows: 10 minutes prior to the effort, and 3 and 30 minutes after its completion. Cortisol (C), testosterone (T), and glucose (G) were determined in the serum, while lactate (LA) was determined in the blood. In addition, prior to each effort, serum creatine kinase (CK) and urea (U) was determined. Directly after each effort, the perception of fatigue (PF) was rated. Results: G, C, and T during official matches were significantly higher than those during non-contact drills. Post-event G, C, T, and LA were higher compared to pre-event values. Conclusions: An official boxing match produced higher stress than a drill of the same time-profile and similar modality. Changes in blood indices corresponded well with the perception of fatigue.

Usefulness of serial determinations of myoglobin and creatine kinase in serum compared for assessment of acute myocardial infarction.

1983 ◽  
Vol 29 (3) ◽  
pp. 469-473 ◽  
Author(s):  
J A Cairns ◽  
E Missirlis ◽  
W H Walker
Keyword(s):  
Myocardial Infarction ◽  
Creatine Kinase ◽  
Total Duration ◽  
Serum Creatine Kinase ◽  
Normal Mean ◽  
Serial Blood Sampling ◽  
The Mean ◽  
Mean Time ◽  
First Myocardial Infarction

Abstract Twenty-one patients with their first myocardial infarction underwent serial blood sampling every 2 h for determination of serum creatine kinase (CK) and myoglobin during the first 48-72 h after onset of pain. The first blood sample, obtained at a mean time of 4.4 h after infarct onset, invariably showed increased myoglobin (mean, 8.3-fold normal), whereas CK was often normal (mean, 1.6-fold normal). Peak myoglobin values occurred earlier than peak CK values (9.9 h vs 21.6 h, p less than 0.0005), but there was a significant correlation of peak values (myoglobin = 0.384CK - 0.264, r = 0.794, p less than 0.0005). The mean exponential disappearance rate (Kd) of CK was 0.00106 min-1 and of myoglobin was 0.00265 min-1 (p less than 0.0005). The disappearance of myoglobin was well described by a mono-exponential expression except in two patients. The total duration of the increase in myoglobin was significantly less than that of CK (34.7 h vs 74.4 h, p less than 0.0005).

Evaluation of a New Procedure for Measuring Serum Creatine Kinase Activity

1970 ◽  
Vol 16 (5) ◽  
pp. 370-374 ◽  
Author(s):  
J Henry Wilkinson ◽  
B Steciw
Keyword(s):  
Creatine Kinase ◽  
Spectrophotometric Method ◽  
Kinase Activity ◽  
Creatine Phosphate ◽  
Sample Volume ◽  
Creatine Kinase Activity ◽  
Serum Creatine Kinase ◽  
Normal Range ◽  
Glucose 6 Phosphate Dehydrogenase

Abstract A new spectrophotometric microtechnique for the determination of serum creatine kinase activity, in which all reagents are provided in a single com-pressed tablet, has been evaluated. The procedure depends upon coupling the creatine phosphate-ADP reaction with the hexokinase and glucose-6-phosphate dehydrogenase reactions. The new technique is quick, relatively simple, and gives results which compare favorably with the conventional spectrophotometric method in precision and sensitivity. It requires a sample volume of 10 Al, and values ranging from 10 to1600 U/liter can be determined without dilution. Gross hemolysis leads to erroneously high values, but the error is negligible with slightly hemolyzed specimens. A provisional normal range has been established

scholarly journals Agreement between fingertip-capillary and antecubital-venous appetite-related peptides

2014 ◽  
Vol 3 (4) ◽  
pp. 233-242 ◽  
Author(s):  
Benjamin Paul Green ◽  
Javier Thomas Gonzalez ◽  
Kevin Thomas ◽  
Emma Stevenson ◽  
Penny Louise Sheena Rumbold
Keyword(s):  
Venous Blood ◽  
Capillary Blood ◽  
Blood Sampling ◽  
Systematic Bias ◽  
Deming Regression ◽  
Methodological Approaches ◽  
Capillary Blood Sampling ◽  
Venous Blood Sampling ◽  
Good Agreement

This study examined the agreement between fingertip-capillary and antecubital-venous measures of appetite-related peptides. Simultaneous fingertip-capillary and antecubital-venous blood samples were collected from 19 participants. The samples were obtained at baseline, 30, 60, 90, and 120 min following breakfast for the determination of plasma GLP17–36, glucagon, insulin and leptin. Between-day reproducibility of fingertip-capillary-derived estimates was assessed in 18 participants. Deming regression, limits of agreement (LOA) and typical error as a coefficient of variation (CV) were used to quantify agreement (CVa) and reproducibility (CVr). Deming regression revealed no systematic bias for any of the analytes studied, but for insulin there was evidence of a proportional difference at higher concentrations. Measures of GLP17–36 (CVa=24.0%, LOA ±2.5 pg m/l per h), leptin (CVa=9.0%, LOA ×/÷1.19) and glucagon (CVa=21.0%, LOA, ±31.5 pg m/l per h) revealed good agreement between methodological approaches. Fingertip-capillary glucagon was highly reproducible between days (CVr=8.2%). GLP17–36 and leptin demonstrated modest reproducibility (CVr=22.7 and 25.0% respectively). For insulin, agreement (CVa=36.0%, LOA ×/÷1.79) and reproducibility were poor (CVr=36.0%). Collectively, the data demonstrate that fingertip-capillary blood sampling provides a comparable and reproducible alternative to antecubital-venous blood sampling for the quantification of glucagon, and to a lesser extent for GLP17–36 and leptin. Caution should be exercised when utilising fingertip-capillary blood sampling for insulin quantification, and consequently should not be employed interchangeably with antecubital-venous blood sampling.

Automated Method for the Determination of Serum Creatine Kinase Activity

1967 ◽  
Vol 13 (3) ◽  
pp. 233-241 ◽  
Author(s):  
Gerard A Fleisher
Keyword(s):  
Creatine Kinase ◽  
Alkaline Solution ◽  
Kinase Activity ◽  
Adenosine Diphosphate ◽  
Creatine Phosphate ◽  
Creatine Kinase Activity ◽  
Serum Creatine Kinase ◽  
Colorimetric Determination ◽  
Automated Method

Abstract An automated (AutoAnalyzer) method for the colorimetric determination of creatine kinase activity in serum is described. This method includes reactivation of creatine kinase with cysteine, incubation of the active enzyme with creatine phosphate and adenosine diphosphate at 37.5°, and subsequent inactivation of enzyme and binding of cysteine by phenylmercuric borate. The enzymatically produced creatine is dialyzed against a solution of diacetyl and reacted with α-naphthol in an alkaline solution. The absorbance of the colored end product is measured at 550 mµ. Individual blanks are determined in the absence of adenosine diphosphate. Comparison of results obtained by this method and a manual procedure shows satisfactory agreement.

Interference by creatine with determination of serum creatine kinase.

1976 ◽  
Vol 22 (10) ◽  
pp. 1741-1743
Author(s):  
R W Lent ◽  
H M Goldschmidt ◽  
D J Adler
Keyword(s):  
Creatine Kinase ◽  
Serum Creatine Kinase
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