scholarly journals Genetic characterisation and local genotypes of canine parvovirus strains collected from pet dogs in central and eastern China during 2018–2019

2020 ◽  
Vol 64 (4) ◽  
pp. 477-486
Author(s):  
Wen Hu ◽  
Liangyan Zheng ◽  
Xin Xu ◽  
Qiang Liu ◽  
Jun Ji ◽  
...  
Keyword(s):  
Eastern China ◽  
Canine Parvovirus ◽  
Zhejiang Province ◽  
Central China ◽  
High Morbidity ◽  
Faecal Samples ◽  
Amino Acid Mutations ◽  
Canine Parvovirus Type 2 ◽  
Major Amino Acid

AbstractIntroductionCanine parvovirus type-2 (CPV-2) causes acute infectious diseases in puppies, which show high morbidity and mortality. Better effect of vaccination against these diseases could be achieved with deeper knowledge of CPV-2 genotype dissemination and mutation history. This study investigated CPV-2–positive samples collected recently over a wide region of China.Material and MethodsA total of 118 faecal samples from dogs identified as CPV-positive were collected from veterinary clinics in central and eastern China. Overall, 16 strains collected from Anhui, 29 from Henan, and 16 from Zhejiang Province were sequenced to determine the genotypic composition of CPV-2 and mutational complexity of CPV-VP2.ResultsThe CPV-2a, CPV-2b, and CPV-2c genotypes were detected in Anhui and Henan Provinces, while CPV-2c alone was detected in Zhejiang Province. Sequence analysis of all strains showed 98.5%–99.8%, 98.3%–99.9%, and 98.7%–99.8% identity among the 16 Anhui, 29 Henan, and 16 Zhejiang strains, respectively. Strains collected from Anhui and Henan Provinces showed lower identity (97.0%), suggesting greater genetic divergence in central China. The mutation rates of Henan and Anhui strains were lower than that of Zhejiang strains. Major amino acid mutations occurred at sites 5, 370, 426, and 440. Epitope and entropy analyses implied these sites’ likely conformance to the principles of mutation tendency, complexity, and diversity.ConclusionThe findings for the evolutionary structure of CPV-2 strains collected from three provinces in central and eastern China advance trend monitoring of the genetic variation in canine parvovirus and point to its implications in the development of novel vaccines.

scholarly journals Molecular characterisation and genetic diversity of canine parvovirus type 2 prevalent in Central China

2020 ◽  
Vol 64 (3) ◽  
pp. 347-354 ◽  
Author(s):  
Wen Hu ◽  
Xin Xu ◽  
Qiang Liu ◽  
Jun Ji ◽  
Yunchao Kan ◽  
...  
Keyword(s):  
Structural Model ◽  
Canine Parvovirus ◽  
Central China ◽  
Clinical Samples ◽  
The Common ◽  
Wild Dogs ◽  
Canine Parvovirus Type 2 ◽  
Reference Strains ◽  
Selection Of

AbstractIntroductionCanine parvovirus (CPV) disease is one of the most threatening to domestic and wild dogs.Material and MethodsA total of 132 clinical samples were isolated from domestic dogs with diarrhoea from Henan, Hubei, Jiangsu, and Anhui provinces from 2016 to 2017, and 56 were positive for CPV-2 by PCR. A phylogenetic tree was constructed for the isolate sequences incorporating 53 non-Chinese reference strains.ResultsVP2 sequences showed the strains mainly to be new CPV-2a/2b and CPV-2c genotypes. The Ala5Gly, Phe267Tyr, Ser297Ala, Tyr324Ile, Gln370Arg, Asn426Asp or Asn426Glu, and Thr440Ala sites in the VP2 protein antigenic region were found to have high mutation rates. The VP2 tertiary structural model shows that the change at these mutation points is a factor for the changes in the protein structure. Significant differences between the Central Chinese strains and others were found, indicating that evolution is geographically related and extended in major regions. The homology between the identified strains confirmed their relationship. Phylogenetic analysis indicated that the common genotypes in the same clusters differ slightly in homology and evolutionary history.ConclusionThis epidemiological study enriches the available data and serves as an important reference for studies on the evolution of CPV and selection of vaccines in China.

scholarly journals Risk and Environmental Factors Associated with the Presence of Canine Parvovirus Type 2 in Diarrheic Dogs from Thessaly, Central Greece

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 590
Author(s):  
Maria Kantere ◽  
Labrini V. Athanasiou ◽  
Alexios Giannakopoulos ◽  
Vassilis Skampardonis ◽  
Marina Sofia ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Clinical Symptoms ◽  
Gastrointestinal Disease ◽  
Environmental Parameters ◽  
Canine Parvovirus ◽  
Geographical Information ◽  
Factors Associated ◽  
Working Dogs ◽  
Canine Parvovirus Type 2

Canine parvovirus type 2 (CPV-2) primarily infects dogs, which are the main host reservoir, causing severe gastrointestinal disease associated with immunosuppression. The present study was conducted in Thessaly, Greece and aimed to identify risk and environmental factors associated with CPV-2 infection in diarrheic dogs. Fecal samples were collected from 116 dogs presenting diarrhea and were tested by polymerase chain reaction (PCR) for the presence of CPV-2 DNA. Supplementary data regarding clinical symptoms, individual features, management factors and medical history were also gathered for each animal during clinical evaluation. Sixty-eight diarrheic dogs were found to be positive for the virus DNA in their feces. Statistical analysis revealed that CPV-2 DNA was less likely to be detected in senior dogs, while working dogs, namely hounds and shepherds, had higher odds to be positive for the virus. Livestock density and land uses, specifically the categories of discontinuous urban fabric and of human population density, were identified as significant environmental parameters associated with CPV-2 infection by using Geographical Information System (GIS) together with the Ecological Niche Model (ENM). This is the first description of the environmental variables associated with the presence of CPV-2 DNA in dogs’ feces in Greece.

Isolation of a virus related to canine parvovirus type 2 from a raccoon (Procyon lotor)

2010 ◽  
Vol 166 (1) ◽  
pp. 24-25 ◽  
Author(s):  
S. Kapil ◽  
G. Rezabek ◽  
B. Germany ◽  
L. Johnston
Keyword(s):  
Procyon Lotor ◽  
Canine Parvovirus ◽  
Canine Parvovirus Type 2

scholarly journals Localization of the VP2 Protein of Canine Parvovirus Type 2 on the Baculovirus Envelop and Its Immunogenicity in a Mouse Model

2012 ◽  
Vol 02 (04) ◽  
pp. 178-185 ◽  
Author(s):  
Chih H. Tsai ◽  
Jing Y. Wang ◽  
Xin G. Xu ◽  
De W. Tong ◽  
Hsin Y. Lu ◽  
...  
Keyword(s):  
Mouse Model ◽  
Canine Parvovirus ◽  
Vp2 Protein ◽  
Canine Parvovirus Type 2

scholarly journals A phylogenetic study of canine parvovirus type 2c in midwestern Brazil

2013 ◽  
Vol 33 (2) ◽  
pp. 214-218 ◽  
Author(s):  
Danúbia S. Fontana ◽  
Paulo Ricardo D. Rocha ◽  
Raquel A.S. Cruz ◽  
Letícya L. Lopes ◽  
Andréia L.T. Melo ◽  
...  
Keyword(s):  
Amino Acid ◽  
Genetic Variability ◽  
Dna Sequencing ◽  
Canine Parvovirus ◽  
Phylogenetic Study ◽  
Vp2 Gene ◽  
Hemorrhagic Enteritis ◽  
Canine Parvovirus Type 2 ◽  
Representative Samples

Since the late 1970s, canine parvovirus type 2 (CPV-2) has emerged as a causative agent of fatal severe acute hemorrhagic enteritis in dogs. To date, three antigenic types of CPV-2 were described worldwide (CPV-2a/b/c). This study was conducted to determine the variants of CPV-2 circulating in dogs from the Cuiabá Municipality in Midwestern Brazil. Out of 50 fecal samples, collected between 2009 and 2011, 27 tested positive for CPV-2. A 583 bp fragment of the VP2 gene was amplified by PCR, 13 representative samples were analyzed further by DNA sequencing. All strains were characterized as CPV-2c, displayed a low genetic variability although observed several amino acid substitution. These findings indicated that CPV-2c has been circulating in dogs from the Cuiabá Municipality in Midwestern Brazil.

Genetic characterization of canine parvovirus type 2 subtypes in Maputo, Mozambique

2016 ◽  
Vol 199 (4) ◽  
pp. 543-549 ◽  
Author(s):  
J. Figueiredo ◽  
C. Miranda ◽  
R. Souto ◽  
E. Silva ◽  
J. Fafetine ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Canine Parvovirus ◽  
Canine Parvovirus Type 2

Canine parvovirus type 2 vaccine protects against virulent challenge with type 2c virus

2008 ◽  
Vol 128 (1-2) ◽  
pp. 48-55 ◽  
Author(s):  
N. Spibey ◽  
N.M. Greenwood ◽  
D. Sutton ◽  
W.S.K. Chalmers ◽  
I. Tarpey
Keyword(s):  
Canine Parvovirus ◽  
Virulent Challenge ◽  
Canine Parvovirus Type 2

scholarly journals Detection of infectious canine parvovirus type 2 by mRNA real-time RT-PCR

2007 ◽  
Vol 146 (1-2) ◽  
pp. 202-208 ◽  
Author(s):  
Gabriella Elia ◽  
Alessandra Cavalli ◽  
Costantina Desario ◽  
Eleonora Lorusso ◽  
Maria Stella Lucente ◽  
...  
Keyword(s):  
Real Time ◽  
Canine Parvovirus ◽  
Rt Pcr ◽  
Canine Parvovirus Type 2

scholarly journals A comprehensive study of canine parvoviruses (Carnivore protoparvovirus 1, Carnivore bocaparvovirus 1 and 2) from shelter dogs in Turkey

2021 ◽  
Vol 66 (No. 10) ◽  
pp. 423-430
Author(s):  
H Isidan ◽  
T Turan
Keyword(s):  
Molecular Analysis ◽  
Direct Detection ◽  
Three Samples ◽  
Faecal Samples ◽  
Shelter Dogs ◽  
Minute Virus ◽  
Molecular Sequencing ◽  
Canine Parvovirus Type 2 ◽  
Comprehensive Study

A total of 150 rectal swab samples were collected from diarrheic dogs from the Sivas Municipal Animal Shelter, Turkey in April 2018. While 127 faecal samples were gathered from adults, 23 samples were collected from puppies. Canine parvovirus type 2 (Carnivore protoparvovirus 1), Canine minute virus (Carnivore bocaparvovirus 1) and Canine bocavirus (Carnivore bocaparvovirus 2) were examined by PCR with three sets of novel primers. Some of the amplicons were subjected to molecular sequencing and molecular analysis. Three faecal (2.36%) samples were found to be positive for Carnivore protoparvovirus 1, five faecal samples (3.94%) were found to be positive for Carnivore bocaparvovirus 1 and three samples (2.36%) were found to be positive for Carnivore bocaparvovirus 2 among 127 adult dogs. On the other hand, 14/23 faecal samples (60.87%) were found to be positive for Carnivore protoparvovirus 1, 8/23 faecal samples (34.78%) were found to be positive for Carnivore bocaparvovirus 1, and 6/23 faecal samples (26.09%) were found to be positive for Carnivore bocaparvovirus 2 from the puppies. Moreover, we detected two distinct clades of Carnivore bocaparvovirus 1 and 2 according to the molecular analysis. To the best of our knowledge, this is the first report for the direct detection of Carnivore bocaparvovirus 1 and 2 in Turkey.

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