A virtual instrumentation based protocol for the automated implementation of the inner field compensation method

Open Physics ◽  
2006 ◽  
Vol 4 (3) ◽  
Author(s):  
Loredana Mereuta ◽  
Tudor Luchian
Keyword(s):  
Transmembrane Potential ◽  
Lipid Membrane ◽  
Cell Physiology ◽  
Dipole Potential ◽  
Virtual Instrumentation ◽  
Electrical Parameters ◽  
Membrane Dipole Potential ◽  
Membrane Bound ◽  
The Difference ◽  
Extracellular Ions

AbstractOne influential parameter which mediates interactions between many types of molecules and biological membranes stems from the lumped contributions of the transmembrane potential, dipole potential and the difference in the surface potentials on both sides of a membrane. With relevance to cell physiology, such electrical features of a biomembrane are prone to undergoing changes as a result of interactions with the aqueous surrounding. Among the most useful tools devoted to exploring changes of electrical parameters of a lipid membrane induced by certain extracellular ions, lipid composition, and embedded membrane peptides and proteins, are spectroscopic imaging and the inner field compensation (IFC) method. In this work we layout the principles of a fully computerized version of the IFC method, which makes it more readily available to users. As a direct application, we deployed this improved version of the IFC method to time-resolve changes induced by alamethicin monomers upon membrane dipole potential, following their aggregation within an artificial lipid membrane. Intriguingly, even prior crossing the membrane core, the membrane-bound alamethicin monomers are shown to significantly increase the dipole potential of the monolayer they reside in. Such data further emphasize the yet less-explored interplay between membrane-based protein and peptides, and the membrane dipole potential.

scholarly journals Probing the Lipid Membrane Dipole Potential by Atomic Force Microscopy

2008 ◽  
Vol 95 (11) ◽  
pp. 5193-5199 ◽  
Author(s):  
Yi Yang ◽  
Kathryn M. Mayer ◽  
Nissanka S. Wickremasinghe ◽  
Jason H. Hafner
Keyword(s):  
Atomic Force Microscopy ◽  
Lipid Membrane ◽  
Dipole Potential ◽  
Membrane Dipole Potential ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Membrane Protein Stabilization Strategies for Structural and Functional Studies

Membranes ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 155
Author(s):  
Ekaitz Errasti-Murugarren ◽  
Paola Bartoccioni ◽  
Manuel Palacín
Keyword(s):  
Membrane Proteins ◽  
Membrane Protein ◽  
Lipid Membrane ◽  
Protein Stabilization ◽  
New Drugs ◽  
Cell Physiology ◽  
Protein Preparation ◽  
Functional Studies ◽  
Membrane Protein Stability ◽  
Druggable Targets

Accounting for nearly two-thirds of known druggable targets, membrane proteins are highly relevant for cell physiology and pharmacology. In this regard, the structural determination of pharmacologically relevant targets would facilitate the intelligent design of new drugs. The structural biology of membrane proteins is a field experiencing significant growth as a result of the development of new strategies for structure determination. However, membrane protein preparation for structural studies continues to be a limiting step in many cases due to the inherent instability of these molecules in non-native membrane environments. This review describes the approaches that have been developed to improve membrane protein stability. Membrane protein mutagenesis, detergent selection, lipid membrane mimics, antibodies, and ligands are described in this review as approaches to facilitate the production of purified and stable membrane proteins of interest for structural and functional studies.

Selective arrangement of vesicles on artificial lipid membrane by biotin-avidin interaction

2021 ◽  
Author(s):  
Kai Hashino ◽  
Daiya Mombayashi ◽  
Yuto Nakatani ◽  
Azusa Oshima ◽  
Masumi Yamaguchi ◽  
...  
Keyword(s):  
Lipid Bilayer ◽  
Lipid Bilayers ◽  
Liquid Crystalline ◽  
Lipid Membrane ◽  
Unsaturated Lipid ◽  
Si Substrates ◽  
Phase Domain ◽  
Unsaturated Lipids ◽  
Lipid Mixtures ◽  
The Difference

Abstract Lipid bilayers suspended over microwells on Si substrates are promising platforms for nanobiodevices that mimic cell membranes. Using the biotin-avidin interaction, we have succeeded in selectively arranging vesicles on the freestanding region of a lipid bilayer. When ternary lipid mixtures of saturated lipid, unsaturated lipid, and cholesterol are used, they separate into liquid-order (Lo) and liquid-crystalline (Lα) domains. A freestanding lipid bilayer prefers the Lα-phase over the Lo-phase because of the difference in their flexibility. In addition, the type of biotinylated lipid determines whether it is localized in the Lα-phase domain or the Lo-phase domain. As a result, the biotinylated unsaturated lipids localized in the Lα-phase domain aggregate in the freestanding lipid bilayer, and vesicles labeled with biotin selectively bind to the freestanding lipid bilayer by the biotin-avidin interaction. This technique helps to introduce biomolecules into the freestanding lipid bilayer of nanobiodevices via vesicles.

scholarly journals Membrane dipole potential is sensitive to cholesterol stereospecificity: Implications for receptor function

2014 ◽  
Vol 184 ◽  
pp. 25-29 ◽  
Author(s):  
Suman Bandari ◽  
Hirak Chakraborty ◽  
Douglas F. Covey ◽  
Amitabha Chattopadhyay
Keyword(s):  
Receptor Function ◽  
Dipole Potential ◽  
Membrane Dipole Potential

scholarly journals Differences in Shedding of the Interleukin-11 Receptor by the Proteases ADAM9, ADAM10, ADAM17, Meprin α, Meprin β and MT1-MMP

2019 ◽  
Vol 20 (15) ◽  
pp. 3677 ◽  
Author(s):  
Sammel ◽  
Peters ◽  
Lokau ◽  
Scharfenberg ◽  
Werny ◽  
...  
Keyword(s):  
Biologically Active ◽  
Target Cells ◽  
Matrix Metalloprotease ◽  
Bone Homeostasis ◽  
Inflammatory Conditions ◽  
Stat3 Phosphorylation ◽  
Membrane Bound ◽  
The Difference ◽  
Membrane Type ◽  
Interleukin 11

Interleukin-11 (IL-11) has been associated with inflammatory conditions, bone homeostasis, hematopoiesis, and fertility. So far, these functions have been linked to classical IL-11 signaling via the membrane bound receptor (IL-11R). However, a signaling cascade via the soluble IL-11R (sIL-11R), generated by proteolytic cleavage, can also be induced. This process is called IL-11 trans-signaling. A disintegrin and metalloprotease 10 (ADAM10) and neutrophil elastase were described as ectodomain sheddases of the IL-11R, thereby inducing trans-signaling. Furthermore, previous studies employing approaches for the stimulation and inhibition of endogenous ADAM-proteases indicated that ADAM10, but not ADAM17, can cleave the IL-11R. Herein, we show that several metalloproteases, namely ADAM9, ADAM10, ADAM17, meprin β, and membrane-type 1 matrix metalloprotease/matrix metalloprotease-14 (MT1-MMP/MMP-14) when overexpressed are able to shed the IL-11R. All sIL-11R ectodomains were biologically active and capable of inducing signal transducer and activator of transcription 3 (STAT3) phosphorylation in target cells. The difference observed for ADAM10/17 specificity compared to previous studies can be explained by the different approaches used, such as stimulation of protease activity or making use of cells with genetically deleted enzymes.

scholarly journals Differential Effect of Cholesterol and Its Biosynthetic Precursors on Membrane Dipole Potential

2012 ◽  
Vol 102 (7) ◽  
pp. 1561-1569 ◽  
Author(s):  
Sourav Haldar ◽  
Ravi Kumar Kanaparthi ◽  
Anunay Samanta ◽  
Amitabha Chattopadhyay
Keyword(s):  
Differential Effect ◽  
Dipole Potential ◽  
Membrane Dipole Potential
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