Eight new species of Siphoderina Manter, 1934 (Digenea, Cryptogonimidae) infecting Lutjanidae and Haemulidae (Perciformes) off Australia

AbstractWe report nine species, eight of which are new, of cryptogonimids belonging to Siphoderina Manter, 1934 from the intestine and pyloric caeca of five species of Lutjanidae (Lutjanus adetii, L. argentimaculatus, L. carponotatus, L. fulviflamma and L. russelli) and one species of Haemulidae (Plectorhinchus gibbosus) recovered from Heron and Lizard Islands off the Great Barrier Reef, Moreton Bay and Ningaloo Reef in Western Australia. We also report the metacercariae of two species from an atherinid fish, Atherinomorus capricornensis, from near Heron Island. Morphological analysis of the species reported here was augmented by DNA sequence analyses utilizing data from the internal transcribed spacers (ITS) 1 and 2, large subunit (LSU) and 5.8S nuclear ribosomal DNA to explore the integrity of the species and their biogeographic distributions. The analysis found strong support for the integrity of Siphoderina and found that it is the sister-taxon to Beluesca Miller et Cribb, 2007. Sequencing included multiple replicates and no intraspecific variation was observed between any of the taxa over the rDNA regions examined. Sequence data from the ITS and LSU regions were analysed with that of species of Beluesca, Caulanus Miller et Cribb, 2007, Chelediadema Miller et Cribb, 2007, Latuterus Miller et Cribb, 2007, Neometadena Hafeezullah et Siddiqi, 1970 and Retrovarium Miller et Cribb, 2007 which all also infect lutjanids or haemulids. Some closely related species of Siphoderina infect only distantly related fishes among the haemulids and lutjanids whereas others form clusters in association with clusters of closely related lutjanids. This pattern suggests a history of some co-evolutionary divergence together with significant host switching. Pseudallacanthochasmus Velasquez, 1961 is considered a synonym of Siphoderina and the new combinations S. grandispinus (Velasquez, 1961) n. comb. and S. magnivesiculum (Gaevskaya et Aljoshkina, 1985) n. comb. are proposed. As a result of the new species described here and these new combinations, Siphoderina now contains 43 species, making it by far the largest genus of the Cryptogonimidae.

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The Evolution of Life Modes in Stictidaceae, with Three Novel Taxa

Journal of Fungi ◽

10.3390/jof7020105 ◽

2021 ◽

Vol 7 (2) ◽

pp. 105

Author(s):

Vinodhini Thiyagaraja ◽

Robert Lücking ◽

Damien Ertz ◽

Samantha C. Karunarathna ◽

Dhanushka N. Wanasinghe ◽

...

Keyword(s):

New Species ◽

Large Scale ◽

Sequence Data ◽

Large Subunit ◽

Monte Carlo Sampling ◽

Small Subunit ◽

Sensu Stricto ◽

Internal Transcribed Spacers ◽

Character State ◽

Phenotypic Data

Ostropales sensu lato is a large group comprising both lichenized and non-lichenized fungi, with several lineages expressing optional lichenization where individuals of the same fungal species exhibit either saprotrophic or lichenized lifestyles depending on the substrate (bark or wood). Greatly variable phenotypic characteristics and large-scale phylogenies have led to frequent changes in the taxonomic circumscription of this order. Ostropales sensu lato is currently split into Graphidales, Gyalectales, Odontotrematales, Ostropales sensu stricto, and Thelenellales. Ostropales sensu stricto is now confined to the family Stictidaceae, which includes a large number of species that are poorly known, since they usually have small fruiting bodies that are rarely collected, and thus, their taxonomy remains partly unresolved. Here, we introduce a new genus Ostropomyces to accommodate a novel lineage related to Ostropa, which is composed of two new species, as well as a new species of Sphaeropezia, S. shangrilaensis. Maximum likelihood and Bayesian inference analyses of mitochondrial small subunit spacers (mtSSU), large subunit nuclear rDNA (LSU), and internal transcribed spacers (ITS) sequence data, together with phenotypic data documented by detailed morphological and anatomical analyses, support the taxonomic affinity of the new taxa in Stictidaceae. Ancestral character state analysis did not resolve the ancestral nutritional status of Stictidaceae with confidence using Bayes traits, but a saprotrophic ancestor was indicated as most likely in a Bayesian binary Markov Chain Monte Carlo sampling (MCMC) approach. Frequent switching in nutritional modes between lineages suggests that lifestyle transition played an important role in the evolution of this family.

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Xanthagaricus siamensis sp. nov. (Agaricaceae), a new species with dull green lamellae from northern Thailand

Phytotaxa ◽

10.11646/phytotaxa.437.1.2 ◽

2020 ◽

Vol 437 (1) ◽

pp. 14-22

Author(s):

YUAN S. LIU ◽

JIAN-KUI (JACK) LIU ◽

PHONGEUN SYSOUPHANTHONG ◽

KEVIN D. HYDE ◽

SAISAMORN LUMYONG

Keyword(s):

New Species ◽

Sequence Data ◽

Large Subunit ◽

Morphological Characteristics ◽

Molecular Data ◽

Nuclear Ribosomal Dna ◽

Molecular Evidence ◽

Internal Transcribed Spacer Region ◽

Northern Thailand ◽

A New Species

Xanthagaricus siamensis, a new species in the family Agaricaceae, was discovered in northern Thailand and is here introduced based on its morphological features and molecular data. It is characterized by small to medium-sized basidiomata, a convex to plano-convex with depressed center pileus when mature, the presence of greyish orange to violet-brown fibrillose squamules on the pileus, lamellae that start out white, change to pinkish white when damaged, then change to dull green with age. Additionally, the annulus is fugacious, and the pileipellis is recognized as a cutis which morphologically distinguishes it from all other known Xanthagaricus. Phylogenetic analysis of the internal transcribed spacer region (ITS) and the large subunit of the nuclear ribosomal DNA (nrLSU) sequence data indicated that the three specimens of X. siamensis form a distinct lineage within Xanthagaricus, and they formed a well-supported clade representing the genus Xanthagaricus. Detailed illustrations of macro- and micro-morphological characteristics and descriptions are provided, as well as other relevant molecular evidence.

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Clitopilus lampangensis (Agaricales, Entolomataceae), a new species from northern Thailand

MycoKeys ◽

10.3897/mycokeys.58.36307 ◽

2019 ◽

Vol 58 ◽

pp. 69-82

Author(s):

Jaturong Kumla ◽

Nakarin Suwannarach ◽

Witchaphart Sungpalee ◽

Kriangsak Sri-Ngernyuang ◽

Saisamorn Lumyong

Keyword(s):

New Species ◽

Rna Polymerase Ii ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Internal Transcribed Spacers ◽

Nuclear Ribosomal Dna ◽

Full Description ◽

Northern Thailand ◽

Molecular Phylogenetic ◽

A New Species

A new species of agaricomycetes, Clitopilus lampangensis, is described based on collections from northern Thailand. This species was distinguished from previously described Clitopilus species by its pale yellow to grayish yellow pileus with the presence of wider caulocystidia. Molecular phylogenetic analyses, based on the data of the internal transcribed spacers (ITS) and the large subunit (LSU) of the nuclear ribosomal DNA, and the second largest subunit of RNA polymerase II (rbp2) genes, also support the finding that C. lampangensis is distinct from other species within the genus Clitopilus. A full description, color photographs, illustrations and a phylogenetic tree showing the position of C. lampangensis are provided.

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Morphology and phylogeny of a new species, Pseudocercospora haldinae (Mycosphaerellaceae) on Haldina cordifolia from India

Phytotaxa ◽

10.11646/phytotaxa.501.2.3 ◽

2021 ◽

Vol 501 (2) ◽

pp. 281-292

Author(s):

SANJAY YADAV ◽

SANJEET KUMAR VERMA ◽

VINAY KUMAR SINGH ◽

RAGHVENDRA SINGH ◽

ARCHANA SINGH ◽

...

Keyword(s):

New Species ◽

Internal Transcribed Spacer ◽

Sequence Data ◽

Large Subunit ◽

Closely Related Species ◽

Host Genus ◽

Rdna Sequence Data ◽

Agar Media ◽

Characteristic Features ◽

A New Species

Pseudocercospora haldinae, a new anamorphic foliicolous hyphomycetous fungus discovered on living leaves of Haldina cordifolia (Rubiaceae) is taxonomically described and illustrated. This species is compared with closely related species of Pseudocercospora and other dematiaceous cercosporoid forms reported on the same host genus. The phylogeny of this species has been inferred from partial nuclear ribosomal 28S large subunit (LSU) and complete internal transcribed spacer (ITS) rDNA sequence data. On the basis of LSU, P. haldinae represents characteristic features of Pseudocercospora s. str. and did not form red crystals when cultivated on agar media.

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Phylogeny and PCR Identification of Clinically Important Zygomycetes Based on Nuclear Ribosomal-DNA Sequence Data

Journal of Clinical Microbiology ◽

10.1128/jcm.37.12.3957-3964.1999 ◽

1999 ◽

Vol 37 (12) ◽

pp. 3957-3964 ◽

Cited By ~ 124

Author(s):

Kerstin Voigt ◽

Elizabeth Cigelnik ◽

Kerry O'donnell

Keyword(s):

Dna Sequence ◽

Ribosomal Dna ◽

Dna Sequences ◽

Sequence Data ◽

Large Subunit ◽

Gene Tree ◽

Strong Support ◽

Nuclear Ribosomal Dna ◽

Syncephalastrum Racemosum ◽

Dna Sequence Data

A molecular database for all clinically important Zygomycetes was constructed from nucleotide sequences from the nuclear small-subunit (18S) ribosomal DNA and domains D1 and D2 of the nuclear large-subunit (28S) ribosomal DNA. Parsimony analysis of the aligned 18S and 28S DNA sequences was used to investigate phylogenetic relationships among 42 isolates representing species of Zygomycetes reported to cause infections in humans and other animals, together with commonly cultured contaminants, with emphasis on members of the Mucorales. The molecular phylogeny provided strong support for the monophyly of the Mucorales, exclusive of Echinosporangium transversale andMortierella spp., which are currently misclassified within the Mucorales. Micromucor ramannianus, traditionally classified within Mortierella, and Syncephalastrum racemosum represent the basal divergences within the Mucorales. Based on the 18S gene tree topology, Absidia corymbiferaand Rhizomucor variabilis appear to be misplaced taxonomically. A. corymbifera is strongly supported as a sister group of the Rhizomucor miehei-Rhizomucor pusillusclade, while R. variabilis is nested withinMucor. The aligned 28S sequences were used to design 13 taxon-specific PCR primer pairs for those taxa most commonly implicated in infections. All of the primers specifically amplified DNA of the size predicted based on the DNA sequence data from the target taxa; however, they did not cross-react with phylogenetically related species. These primers have the potential to be used in a PCR assay for the rapid and accurate identification of the etiological agents of mucormycoses and entomophthoromycoses.

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Genetic characterization of members of the genus Contracaecum (Nematoda: Anisakidae) from fish-eating birds from west-central Florida, USA, with evidence of new species

Parasitology ◽

10.1017/s003118200700251x ◽

2007 ◽

Vol 134 (7) ◽

pp. 1041-1051 ◽

Cited By ~ 26

Author(s):

S. D'AMELIO ◽

N. B. BARROS ◽

S. INGROSSO ◽

D. A. FAUQUIER ◽

R. RUSSO ◽

...

Keyword(s):

New Species ◽

Sequence Data ◽

Rflp Analysis ◽

Distinct Group ◽

Small Subunit ◽

Internal Transcribed Spacers ◽

Nuclear Ribosomal Dna ◽

Central Florida ◽

Pcr Rflp ◽

Distinct Lineage

SUMMARYSpecimens of Contracaecum spp. from Phalacrocorax auritus and Pelecanus occidentalis from Florida were characterized by sequencing of the small subunit of the mitochondrial ribosomal RNA gene (rrnS) and by PCR-based RFLP analysis of the same gene and of the internal transcribed spacers (ITS) of nuclear ribosomal DNA. Analyses of the rrnS sequence data using the MP and UPGMA approaches yielded trees with similar topologies, delineating 3 main clusters. Specimens from Ph. auritus, morphologically assigned to C. rudolphii (s.l.), were part of the cluster comprising also the other 2 species of the C. rudolphii complex (A and B), but representing a genetically distinct group, potentially corresponding to a distinct lineage within the complex, provisionally named as C. rudolphii C. The second cluster comprised 5 individuals from P. occidentalis, which formed a genetically relatively homogeneous group. The rrnS data indicate that these specimens (indicated as Contracaecum sp. 1) are clearly genetically different from the morphologically most closely related species, i.e. C. rudolphii (s.l.) and C. microcephalum, and could represent a new species. The third cluster comprised 7 specimens from P. occidentalis morphologically assigned to C. multipapillatum (s.l.). These were shown to be genetically homogeneous and related to but quite distinct from C. multipapillatum from Greece, although additional studies are needed to assess their status. PCR-RFLP based markers for the quick identification of these taxa are provided.

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Two new species of Verruconis from Hainan, China

MycoKeys ◽

10.3897/mycokeys.48.32147 ◽

2019 ◽

Vol 48 ◽

pp. 41-53 ◽

Cited By ~ 3

Author(s):

Min Qiao ◽

Weiguang Tian ◽

Rafael F. Castañeda-Ruiz ◽

JianPing Xu ◽

Zefen Yu

Keyword(s):

New Species ◽

Dna Sequences ◽

Sequence Data ◽

Nuclear Ribosomal Dna ◽

Closely Related Species ◽

Protein Coding ◽

Dna Sequence Data ◽

Protein Coding Genes ◽

Two New Species ◽

New Perspective

Two new species of the genusVerruconis,V.hainanensisandV.pseudotricladiata, were described using combined morphological and DNA sequence data. The DNA sequences of respective strains including nuclear ribosomal DNA genes (nuSSU, ITS, nuLSU) and fragments of three protein-coding genes (ACT1, BT2, TEF1) were sequenced and compared with those from closely-related species to generaOchroconisandVerruconis(Family Sympoventuriaceae, Order Venturiales). Morphologically, both species showed typical ampulliform conidiophores and conidiogenous cells, features not seen in other species ofVerruconis. The conidia ofV.hainanensisare fusiform and those ofV.pseudotricladiataare Y or T shaped, similar to old members of a closely-related genusScolecobasidium. The addition of these two new species provides a new perspective on the heterogeneity ofScolecobasidium.

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Pleurotus sirindhorniae (Pleurotaceae, Agaricales), a new species from northern Thailand

Phytotaxa ◽

10.11646/phytotaxa.460.4.6 ◽

2020 ◽

Vol 460 (4) ◽

pp. 285-295

Author(s):

NAKARIN SUWANNARACH ◽

JATURONG KUMLA ◽

KANITTA SATIENPERAKUL ◽

WITCHAPHART SUNGPALEE ◽

KRIANGSAK SRI-NGERNYUANG ◽

...

Keyword(s):

New Species ◽

Rna Polymerase Ii ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Elongation Factor ◽

Morphological Characteristics ◽

Internal Transcribed Spacers ◽

Nuclear Ribosomal Dna ◽

Full Description ◽

Northern Thailand

A new saprophytic fungus, Pleurotus sirindhorniae, is described based on collections from northern Thailand. This species is characterized by greyish yellow to olive brown pileus, ellipsoid to elongate basidiospores and clavate to mucronate cheilocystidia. It is distinguished from the previously described Pleuotus species by its distinct morphological characteristics. Molecular phylogenetic analyses of the large subunit (LSU) and internal transcribed spacers (ITS) of the nuclear ribosomal DNA (rDNA), the translation elongation factor 1-alpha (tef1) and the RNA polymerase II largest subunit (rpb1) genes also support P. sirindhorniae as a distinct new species within the genus Pleurotus. A full description, color photographs, illustrations and a phylogenetic tree to show the position of P. sirindhorniae are provided.

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Gyrodon suthepensis (Boletales, Basidiomycota), a new ectomycorrhizal fungus from northern Thailand and its ecomycorhizal association

Phytotaxa ◽

10.11646/phytotaxa.321.2.3 ◽

2017 ◽

Vol 321 (2) ◽

pp. 181

Author(s):

JATURONG KUMLA ◽

NAKARIN SUWANNARACH ◽

SANTHITI VADTHANARAT ◽

SAISAMORN LUMYONG

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Morphological Characteristics ◽

Ectomycorrhizal Fungus ◽

Internal Transcribed Spacers ◽

Nuclear Ribosomal Dna ◽

Full Description ◽

Northern Thailand ◽

Mycorrhizal Association

A new ectomycorrhizal fungus, Gyrodon suthepensis, is described based on collections from northern Thailand. It can be distinguished from the previously described Gyrodon species by the morphological characteristics. The molecular phylogenetic analyses of the internal transcribed spacers (ITS) and large subunit (LSU) of the nuclear ribosomal DNA confirms the position of the new species within the family Paxillaceae. The full description, color photographs, illustrations and the phylogenetic tree to show the position of G. suthepensis are provided. The new species grows in association with the ectomycorrhizal tree Betula alnoides, and the morphology and anatomy of mycorrhizas are described. Moreover, the identification of mycorrhizal association of G. suthepensis was confirmed by molecular methods.

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Two new cryptogonimid genera Beluesca n. gen. and Chelediadema n. gen. (Digenea: Cryptogonimidae) from tropical Indo-West Pacific Haemulidae (Perciformes)

Zootaxa ◽

10.11646/zootaxa.1543.1.2 ◽

2007 ◽

Vol 1543 (1) ◽

pp. 45-60 ◽

Cited By ~ 13

Author(s):

TERRENCE L. MILLER ◽

THOMAS H. CRIBB

Keyword(s):

Dna Sequence ◽

Host Preference ◽

Sequence Data ◽

Large Subunit ◽

Internal Transcribed Spacers ◽

Nuclear Ribosomal Dna ◽

Minimum Evolution ◽

West Pacific ◽

Pyloric Caeca ◽

Dna Sequence Data

A survey of the parasites of Indo-West Pacific Haemulidae revealed the presence of three new cryptogonimid (Digenea: Cryptogonimidae) species warranting two new genera, Beluesca littlewoodi n. gen., n. sp. and B. longicolla n. sp. from the intestine and pyloric caeca of Plectorhinchus gibbosus and Chelediadema marjoriae n. gen., n. sp. from the intestine and pyloric caeca of Diagramma labiosum, P. albovittatus and P. gibbosus from Heron and Lizard Islands off the Great Barrier Reef, Australia. Beluesca n. gen. is distinguished from all other cryptogonimid genera by the combination of an elongate body, funnel-shaped oral sucker, relatively small number of large oral spines, highly lobed ovary, opposite to slightly oblique testes, uterine loops that are restricted to the hindbody and extend well posterior to the testes and vitelline follicles that may extend from the ovary into the forebody, but do not extend anterior to the intestinal bifurcation. Pseudallacanthochasmus plectorhynchi Mamaev, 1970 is transferred to Beluesca as B. plectorhyncha (Mamaev, 1970) n. comb. based on morphological and ecological (host preference) characteristics. Chelediadema n. gen. is distinguished from all other cryptogonimid genera by the combination of a lanceolate body, relatively small number of large oral spines, prepharynx that is much longer than the oesophagus, tandem testes, uterine loops that are extensive in the hindbody and extend well posterior to the testes and vitelline follicles that extend from the ovary to the pharynx. Morphological analysis of the three species described here was augmented with DNA sequence analyses utilizing data from the large subunit (LSU) and the internal transcribed spacers (ITS) 1 and 2, and 5.8S nuclear ribosomal DNA. Sequence data from the LSU and ITS (encompassing the ITS1, 5.8S and ITS2) of the taxa examined here were aligned with those reported for other cryptogonimids, Caulanus thomasi, Latuterus tkachi, Neometadena ovata, four representative species of Retrovarium and an undescribed species of Siphoderina, for comparative purposes and to explore levels of interspecific and intergeneric variation among these taxa. Minimum evolution analysis was conducted on a combined (LSU and ITS) dataset to explore relationships among these genera. Despite their superficial morphological and host preference similarities, species of Beluesca and Chelediadema were genetically distant from each other. Interspecific and intergeneric variation among the species described here is similar to that reported for other cryptogonimids.

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