scholarly journals Fuhrmanolepis beskydensis n.sp. (Cestoda: Dilepididae) from woodcock Scolopax rusticola L.(Aves, Charadriformes) in Slovakia with comments on sytematics and nomenclature of the genus Fuhrmanolepis Spassky et Spasskaya, 1965

2008 ◽  
Vol 45 (4) ◽  
pp. 173-180 ◽  
Author(s):  
J. Macko ◽  
A. Macková ◽  
S. Sabolová-Barl’áková
Keyword(s):  
Light Microscope ◽  
Microscope Observation ◽  
Scolopax Rusticola ◽  
Single Crown ◽  
Lateral Margins

AbstractFuhrmanolepis beskydensis n.sp. from woodcock, Scolopax rusticola L. in Slovakia is described based upon light microscope observation. The medium sized species possess a single crown of 21‘diorchid’ (wrench-shaped) hooks, 28–30 μm long. Irregularly alternating genital pores were combined with abnormal multiple shifting of pores within the lateral margins of strobila. Number of testes 18–25. Cirrus-sac and evaginated cirrus are 115–135 × 7–12 and 26 × 6–11 μm, respectively. The species is differentiated from closely related congeneric taxons and some other morphologically similar dilepidids. An attention is being paid to taxonomy and nomenclature of Fuhrma n olepis Spassky et Spaskaja, 1965 regarding an emendation of mentioned genus to Fuhrma nn olepis by Bona (1994a) and modification of its diagnosis.

scholarly journals Fuhrmanolepis dubinskyi n. sp. (Cestoda: Dilepididae) from woodcock Scolopax rusticola (L.) (Aves, Charadriiformes) in Slovakia

2008 ◽  
Vol 45 (3) ◽  
pp. 130-133 ◽  
Author(s):  
J. Macko ◽  
V. Hanzelová ◽  
V. Dudiňák
Keyword(s):  
Light Microscope ◽  
New Taxon ◽  
Congeneric Species ◽  
Scolopax Rusticola ◽  
Single Crown

AbstractDilepidid cestode Fuhrmanolepis dubinskyi n. sp. from woodcock, Scolopax rusticola L. in Slovakia is described on the basis of light microscope observations. The new taxon is characterised by medium sized strobila, single crown of ten, 47–53 μm long “diorchid” (wrench-shaped) rostellar hooks. Genital pores alternate irregularly. Number of testes is 20–30 and measurements of cirrus-sac and evaginated cirrus reach 176 − 217 × 18 − 31 and 38 − 56 × 16 − 21 μm, respectively. The uterus is reticular and eggs possess spiny embryophore. F. dubinskyi is differentiated from closely related congeneric species and some other morphologically similar dilepidids.

Preparation and flattening of thick epoxy sections for light microscopy

1976 ◽  
Vol 54 (20) ◽  
pp. 2405-2407 ◽  
Author(s):  
Thompson Demetrio Pizzolato
Keyword(s):  
Light Microscopy ◽  
Light Microscope ◽  
Microscope Observation ◽  
Detergent Solution

A technique is described which allows 4- to 10-μm-thick plastic sections to be routinely flattened for light microscope observation. Samples are fixed in Karnovsky's fluid, embedded in Spurr's resins, and cut with a steel knife on a rotary microtome. Sections are flattened by passage through xylene, ethanol, and a detergent solution, then dried on slides, and mounted in immersion oil and hexane. Sections may either be stained before mounting or examined unstained.

A simple protocol for paraffin‐embedded myelin sheath staining with osmium tetroxide for light microscope observation

2008 ◽  
Vol 71 (7) ◽  
pp. 497-502 ◽  
Author(s):  
Federica Di Scipio ◽  
Stefania Raimondo ◽  
Pierluigi Tos ◽  
Stefano Geuna
Keyword(s):  
Myelin Sheath ◽  
Light Microscope ◽  
Osmium Tetroxide ◽  
Microscope Observation ◽  
Simple Protocol

Comparative assessment of pollen micromorphology of Salvia assurgens (Lamiaceae), an endemic sage from Mexico

Phytotaxa ◽  
2020 ◽  
Vol 458 (3) ◽  
pp. 183-194
Author(s):  
BRENDA Y. BEDOLLA-GARCÍA ◽  
MAYRA CASTRO-MORALES ◽  
CARLOS A. CULTID-MEDINA
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Data Analysis ◽  
Light Microscope ◽  
Pollen Grains ◽  
Comparative Assessment ◽  
Microscope Observation ◽  
Pollen Characters ◽  
Small Pollen ◽  
Scanning Electron

The present study provides novel information about the pollen of Salvia assurgens. Pollen grains were collected and described based on their observed characters by light microscope and scanning electron microscopy. The species is distinguished from other Mexican salvias by having small pollen grains (14.2 × 18.2 µm), thin primary muri (0.2 µm thick), elongated primary lumina (1.03 µm long) and secondary lumina with relatively few perforations (9, range 5–14). Pollen characters are similar to those of the majority of American salvias. Regarding Mexican sages, there has been little palynological research, and only 23 species of 32 examined have been quantitatively studied. Standardization is needed in different aspects of palynological studies, especially in relation to measurement protocols and data analysis, as well as the increased use of scanning electron microscopy (SEM), since the majority of differences among species are provided by SEM microscope observation.

GEOLOGY OF CU-MO SIERRA GORDA DEPOSIT, NORTH CHILE

2017 ◽  
pp. 0-0
Author(s):  
Jadwiga PIECZONKA ◽  
Adam PIESTRZYŃSKI ◽  
Władysław ZYGO
Keyword(s):  
Light Microscope ◽  
High Variability ◽  
Microscope Observation ◽  
Accessory Minerals ◽  
Reflected Light

Samples collected during the KGHM PM S.A. program Go Global Internship were used in these work to develop a mineralogical model of all zones in the Sierra Gorda deposit. Reflected light microscope observation and quantitative microchemical analyses show that thedeposit is characterized by high variability of all parameters. The major mineral within the oxides zone is atacamite. The following other minerals have been identified: malachite, brochantite, chryzocolla, sampleite, paratacamite, brushite, antlerite, aubertite, montmorillonite and kaolinite. The major minerals in the sulphide zone are chalcopyrite, covellite, molybdenite and pyrite. The minor and accessory minerals are bornite, arsenopyrite, pyrrhotite, sphalerite and galena. Substitution of Cu in rock-forming minerals as well as inclusions of Cu and Mo sulphides within silicates will play an important role in the recovery of both metals.

A SEM and Light Microscope Study of the Epidermal Leaf Structures of the Carnivorous Plant, Sarracenia purpurea, L.

1971 ◽  
Vol 29 ◽  
pp. 358-359
Author(s):  
B. J. Panessa ◽  
J. F. Gennaro
Keyword(s):  
Methylene Blue ◽  
Toluidine Blue ◽  
Outer Surface ◽  
Microscope Study ◽  
Light Microscope ◽  
Carnivorous Plant ◽  
Sarracenia Purpurea ◽  
Inner Surface

Tissue from the hood and sarcophagus regions were fixed in 6% glutaraldehyde in 1 M.cacodylate buffer and washed in buffer. Tissue for SEM was partially dried, attached to aluminium targets with silver conducting paint, carbon-gold coated(100-500Å), and examined in a Kent Cambridge Stereoscan S4. Tissue for the light microscope was post fixed in 1% aqueous OsO4, dehydrated in acetone (4°C), embedded in Epon 812 and sectioned at ½u on a Sorvall MT 2 ultramicrotome. Cross and longitudinal sections were cut and stained with PAS, 0.5% toluidine blue and 1% azure II-methylene blue. Measurements were made from both SEM and Light micrographs.The tissue had two structurally distinct surfaces, an outer surface with small (225-500 µ) pubescent hairs (12/mm2), numerous stoma (77/mm2), and nectar glands(8/mm2); and an inner surface with large (784-1000 µ)stiff hairs(4/mm2), fewer stoma (46/mm2) and larger, more complex glands(16/mm2), presumably of a digestive nature.

Epoxy Resin Embedment

1968 ◽  
Vol 26 ◽  
pp. 100-101
Author(s):  
J. G. Adams ◽  
M. M. Campbell ◽  
H. Thomas ◽  
J. J. Ghldonl
Keyword(s):  
Electron Microscopy ◽  
Electron Beam ◽  
Epoxy Resin ◽  
Light Microscope ◽  
Epoxy Resins ◽  
Image Contrast ◽  
Tissue Preservation ◽  
Resin System ◽  
Excellent Quality

Since the introduction of epoxy resins as embedding material for electron microscopy, the list of new formulations and variations of widely accepted mixtures has grown rapidly. Described here is a resin system utilizing Maraglas 655, Dow D.E.R. 732, DDSA, and BDMA, which is a variation of the mixtures of Lockwood and Erlandson. In the development of the mixture, the Maraglas and the Dow resins were tested in 3 different volumetric proportions, 6:4, 7:3, and 8:2. Cutting qualities and characteristics of stability in the electron beam and image contrast were evaluated for these epoxy mixtures with anhydride (DDSA) to epoxy ratios of 0.4, 0.55, and 0.7. Each mixture was polymerized overnight at 60°C with 2% and 3% BDMA.Although the differences among the test resins were slight in terms of cutting ease, general tissue preservation, and stability in the beam, the 7:3 Maraglas to D.E.R. 732 ratio at an anhydride to epoxy ratio of 0.55 polymerized with 3% BDMA proved to be most consistent. The resulting plastic is relatively hard and somewhat brittle which necessitates trimming and facing the block slowly and cautiously to avoid chipping. Sections up to about 2 microns in thickness can be cut and stained with any of several light microscope stains and excellent quality light photomicrographs can be taken of such sections (Fig. 1).

Selective Staining of Acid Mucopolysaccharides By Ruthenium Red

1968 ◽  
Vol 26 ◽  
pp. 38-39
Author(s):  
J. H. Luft
Keyword(s):  
Electron Microscope ◽  
Light Microscopy ◽  
Light Microscope ◽  
Osmium Tetroxide ◽  
Single Cells ◽  
Ruthenium Red ◽  
Collagen Fibers ◽  
Selective Staining ◽  
Pectic Substances ◽  
Solid Tissues

Ruthenium red is one of the few completely inorganic dyes used to stain tissues for light microscopy. This novelty is enhanced by ignorance regarding its staining mechanism. However, its continued usefulness in botany for demonstrating pectic substances attests to selectivity of some sort. Whether understood or not, histochemists continue to be grateful for small favors.Ruthenium red can also be used with the electron microscope. If single cells are exposed to ruthenium red solution, sufficient mass can be bound to produce observable density in the electron microscope. Generally, this effect is not useful with solid tissues because the contrast is wasted on the damaged cells at the block surface, with little dye diffusing more than 25-50 μ into the interior. Although these traces of ruthenium red which penetrate between and around cells are visible in the light microscope, they produce negligible contrast in the electron microscope. However, its presence can be amplified by a reaction with osmium tetroxide, probably catalytically, to be easily visible by EM. Now the density is clearly seen to be extracellular and closely associated with collagen fibers (Fig. 1).

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