Seed macro and micro morphology of the selected Nigella (Ranunculaceae) taxa from Turkey and their systematic significance

Biologia ◽  
2009 ◽  
Vol 64 (2) ◽  
Author(s):  
Mehmet Dadandi ◽  
Gamze Kökdil ◽  
Ahmet İlçim ◽  
Başak Özbilgin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Type I ◽  
Type Ii ◽  
Systematic Significance ◽  
Species Type ◽  
Micromorphological Characters ◽  
Micro Morphology ◽  
Mature Seeds ◽  
Scanning Electron

AbstractMacro and micromorphological properties of intact and mature seeds of 12 taxa (species and varieties) belonging to Nigella L. (Ranunculaceae) was investigated using light and scanning electron microscopy. Material studied covers 11 species of 15 Turkish Nigella. Studied taxa were divided into two types. Type I has ovate to orbicular seeds that includes four species. Type II has triquedrous seeds and includes seven species. Type II was subdivided into two. Type IIa has triquedrous to subpyramidal seeds (five species) and Type IIb has triquedrous to subglobose seeds (two species). Further segregation was performed micromorphologically and an identification key of studied Nigella taxa was given. Studied Nigella taxa have a diverse macro and micromorphological characters that utilize to separate them from each other to assess the systematics of Nigella.

scholarly journals Petal and Sepal Epidermal Micromorphology of Six Lathyrus Taxa (Fabaceae) and their Systematic Value

2012 ◽  
Vol 40 (1) ◽  
pp. 35 ◽  
Author(s):  
Hüseyin CILDIR ◽  
Ahmet KAHRAMAN ◽  
Musa DOGAN
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Glandular Trichomes ◽  
Systematic Significance ◽  
Trichome Types ◽  
First Time ◽  
Scanning Electron

In this study, the epidermal types and their distribution on dorsal and lateral petals, the trichome types and their density on sepals of Lathyrus chloranthus, L. digitatus, L. laxiflorus subsp. laxiflorus, L. roseus subsp. roseus, L. sativus, and L. tuberosus, belonging to sections Lathyrus, Lathyrostylis, Pratensis, and Orobon of the genus Lathyrus in Turkey were investigated using light microscopy (LM) and scanning electron microscopy (SEM), and the systematic significance of these characters was evaluated. These taxa, except L. sativus, are studied for the first time under aspects of the petal and sepal micromorphology. Three major epidermal types were recognized on the petal: tabular rugose striate cells (TRS), areolate cells with more or less striations (AS), and papillose conical striate cells (PCS). TRS and AS were further subdivided into three subtypes. TRS was found on the dorsal and lateral petals of L. chloranthus, L. sativus, and L. tuberosus: the dorsal petals of L. roseus subsp. roseus and the lateral petals of L. laxiflorus subsp. laxiflorus, while AS was present on the dorsal and lateral petals of L. digitatus and the lateral petals of L. roseus subsp. roseus. PCS was found only on the dorsal petals of L. digitatus. Three main types of trichomes on the sepal were observed: peltate glandular, capitate glandular, and nonglandular trichomes. The capitate glandular and nonglandular trichomes were further subdivided into three subtypes. The peltate glandular trichomes were present only in L. chloranthus, but absent in the others. The capitate glandular trichomes were found in L. chloranthus, L. laxiflorus subsp. laxiflorus, L. roseus subsp. roseus, and L. tuberosus. The nonglandular trichomes were always present in L. chloranthus and L. laxiflorus subsp. laxiflorus. The present results show that the petal and sepal micromorphology can be used in delimitation of the taxa based on petal and sepal micromorphology.

scholarly journals First definitive record of a whip scorpion Labochirus tauricornis (Pocock, 1900) from Goa, India: with notes on its morphometry and pedipalp micro-morphology

2018 ◽  
Vol 10 (7) ◽  
pp. 11955
Author(s):  
Manoj Ramakant Borkar
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sexual Dimorphism ◽  
Diagnostic Value ◽  
The State ◽  
Diagnostic Data ◽  
Micro Morphology ◽  
Scanning Electron

Bastawade & Borkar in 2008 made a passing reference to the presence of a single uropygid species in Goa, though without much primary diagnostic data on the collected specimen of four females.  The present study puts in place a definitive record of the uropygid, Labochirus tauricornis Pocock, (1900) in the state of Goa, and addresses an important gap in our understanding of its occurrence, morphology, and ecology. Besides documenting the species of this cryptozoic, nocturnal arachnid predator commonly known as ‘Vinegaroon’ on account of their vinegary allomone spray; the present study also describes the gross morphology , morphometry and micro-morphology of non-ambulatory sub-raptorial pedipalps which are of taxonomic-diagnostic value, elucidated using scanning electron microscopy, in addition to routine stereomicroscopy. The paper also examines in detail, sexual dimorphism and morphometry of this uropygid species. 

scholarly journals Seed morphology and anatomy of Hypericum elegans Steph. ex Willd.

2014 ◽  
Vol 35 (1) ◽  
pp. 15-18
Author(s):  
Piotr Szkudlarz
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Eastern Europe ◽  
Seed Coat ◽  
Central And Eastern Europe ◽  
Seed Morphology ◽  
Seed Sample ◽  
Cell Layers ◽  
Mature Seeds ◽  
Scanning Electron

Abstract Hypericum elegans is a rare perennial distributed primarily in Central and Eastern Europe. Seed morphology and anatomy in H. elegans was studied on the basis of a seed sample from its only locality in Poland. Scanning electron microscopy revealed that the seed coat of mature seeds is composed basically of 3 cell layers: epidermal, subepidermal and sclerenchymatic. They are documented graphically here.

Digestion techniques for exposure of smooth muscle cells in cerebral arterioles

1989 ◽  
Vol 47 ◽  
pp. 942-943
Author(s):  
S. Ghoneim ◽  
G. Baumbach
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Smooth Muscle ◽  
Basement Membrane ◽  
Three Dimensional ◽  
Type Ii ◽  
Sprague Dawley ◽  
Packed Red Blood Cells ◽  
Cerebral Arterioles ◽  
Scanning Electron

Scanning electron microscopy has proven to be a useful tool for the quantitation of three- dimensional characteristics of vascular smooth muscle. A major impediment to obtaining useful preparations of cerebral blood vessels for scanning electron microscopy is that smooth muscle in the tunica media is covered by a thin layer of basement membrane and by arachnoid tissue which contains collagen. The goal of this study was to develop a reliable, reproducible method for removing basement membrane and arachnoid tissue from cerebral arterioles, while at the same time leaving the smooth muscle intact.We compared several methods of tissue digestion in pial arterioles in adult Sprague-Dawley rats. The cerebral circulation was perfused with glutaraldehyde (2.5%) in cacodylate buffer (0.1 M) via the ascending aorta and then reinfused with packed red blood cells. In one group of rats, whole brains were immersed in osmium tetroxide (2%) for 2 hours (1 hr at 5°C and 1 hr at 40°C), followed by immersion in HC1 (8 N at 60°C) for 20-25 minutes. In another group, whole brains were dipped briefly in HC1 (8 N at 60°C) and then immersed in collagenase type II (2 mg/ml at 37° C; Sigma) for 12 hours. In a third group of rats, individual arterioles were dissected from the brain with a microsurgical knife, dipped briefly in HC1 (5-10 seconds) or KOH (5M at 60°C for 2-3minutes), and immersed in collagenase type II alone (12 hours), or in combination with collagenase type IV (2 mg/ml; 6-12 hours) and/or pepsin (2 mg/ml; 6-12 hours).

Microwave-assisted synthesis and evaluation of type 1 collagen–apatite composites for dental tissue regeneration

2018 ◽  
Vol 33 (1) ◽  
pp. 103-115 ◽  
Author(s):  
Kanwal Ilyas ◽  
Saba W Qureshi ◽  
Sadia Afzal ◽  
Roquyya Gul ◽  
Muhammad Yar ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Sodium Dodecyl ◽  
Fourier Transform Infrared ◽  
Type I ◽  
Formation Behavior ◽  
Scanning Electron

The aim was to develop an economical and biocompatible collagen-based bioactive composite for tooth regeneration. Acid-soluble collagen was extracted and purified from fish scales. The design was innovated to molecularly tailor the surface charge sites of the nano-apatite providing chemical bonds with the collagen matrix via microwave irradiation technique. The obtained collagen was identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis. The composites were characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis/differential scanning calorimetry, and scanning electron microscopy. MC3T3-E1 cell lines were used to assess the biological effects of these materials by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetra zolium bromide (MTT) assay. Indirect contact test was performed by extracting representative elutes in cell culture media and sulforhodamine B analysis was performed. Chorioallantoic membrane assay was conducted to define the new vessels formation behavior. The purity of collagen extracts was determined and showed two α-chains, i.e. the characteristic of type I collagen. Fourier transform infrared spectroscopy showed the characteristic peaks for amide I, I, III, and phosphate for collagen and composites. Scanning electron microscopy images showed three-dimensional mesh of collagen/apatite nano-fibers. Nontoxic behavior of composites was observed and there were graded and dose-related effects on experimental compounds. The angiogenesis and vessels formation behavior were observed in bioactive collagen composite. The obtained composites have potential to be used for tooth structure regeneration.

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