Effects of sorbitol on the production of phenolic compounds and terpenoids in the cell suspension cultures of Ocimum basilicum L.

Biologia ◽  
2020 ◽  
Author(s):  
Muhammed A. Açıkgöz
1989 ◽  
Vol 8 (2) ◽  
pp. 93-96 ◽  
Author(s):  
Samia Lofty ◽  
Annie Fleuriet ◽  
Teresa Ramos ◽  
Jean-Jacques Macheix

1980 ◽  
Vol 35 (7-8) ◽  
pp. 551-556 ◽  
Author(s):  
K.-H. Knobloch ◽  
J. Berlin

Cell suspension cultures of Catharanthus roseus have been subjected to various media condi­tions in order to stimulate the formation of indole alkaloids. High ajmalicine contents (up to 0.5 mg/g cell fresh weight) were achieved by transferring 2-week-old cell suspensions to a 10-fold volume of a 8% sucrose solution. The alkaloid accumulation started two days after the transfer and reached a plateau after ten days. Furthermore an enhanced level of phenolic compounds was found, whereas growth of the culture was low. The accumulation of both, alkaloids and poly­phenols was stimulated by high concentrations of sucrose and low concentrations of nitrogen con­taining salts and phosphate. When these minerals were added to the sucrose solution in con­centrations commonly used for cell culture media, the accumulation of alkaloids and phenolic compounds was largely suppressed.


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