scholarly journals Analisis Filogenetik Cyprinus carpio Ruang Lingkup Asia Timur Dan Eropa Berdasarkan Genom Mitokondria

2021 ◽  
Vol 8 (1) ◽  
pp. 166
Author(s):  
Erik Nanda Putra ◽  
Abdul Razak ◽  
Ramadhan Sumarmin
Keyword(s):  
Phylogenetic Analysis ◽  
Mitochondrial Genome ◽  
East Asia ◽  
Cyprinus Carpio ◽  
Genomic Dna ◽  
Complete Mitochondrial Genome ◽  
Next Generation Sequencing Technology ◽  
Mirror Carp ◽  
Carp Cyprinus Carpio ◽  
Close Relationship

Carp (Cyprinus carpio L.) is one of the oldest and most commercially cultivated freshwater fish in the World. However, there are still many undetermined phylogenetic relationships and the origins of common goldfish lineages, which are an obstacle to the conservation and genetic reproduction of this species. In the process of phylogenetic analysis, researchers used mitochondrial genomes where the genomic DNA was obtained from homozygous double haploid clonal lines from domesticated Songpu strains, and the total genomic DNA was extracted and sequenced using next-generation sequencing technology. Complete mitochondrial genome sequencing of 11 individuals representing East Asia and Europe and phylogenetic analysis was performed. The purpose of this study is to provide information about the phenology of Cyprinus carpio fish, which is expected to be the basis for increasing understanding to determine the kinship relationship between Cyprinus carpio in East Asia and Europe. The results showed that the relationship between Cyprinus carpio color, Cyprinus carpio songpu mirror carp, Cyprinus carpio carpio, and Cyprinus carpio wuyuanensis was very close, this can be seen from the bootstrap value of 100%. Meanwhile, with Cyprinus carpio haematopterus has a bootstrap value of 70%. Meanwhile, with Cyprinus xingguonensis it has a bootstrap value of 66%. Furthermore, it can also be said that the Songpu mirror goldfish is closely related to C. carpio carpio which is also a member of the genus Cyprinu. The genus Cyprinu has a close relationship with the genus Catla. In the phylogenetic tree, this species of fish forms a very coherent group, supported by a high bootstrap value of 100% and an average genetic distance of 0.02.  Key words: Mitochondrial genome, Phylogenetics, general Cyprinus carpio

scholarly journals The Complete Mitochondrial Genome of the New Zealand Parasitic Blowfly Lucilia sericata (Insecta: Diptera: Calliphoridae)

2020 ◽  
Author(s):  
Nikola Palevich
Keyword(s):  
Phylogenetic Analysis ◽  
New Zealand ◽  
Mitochondrial Genome ◽  
Complete Mitochondrial Genome ◽  
Lucilia Sericata ◽  
Coding Region ◽  
Protein Coding ◽  
Next Generation Sequencing Technology ◽  
Monophyletic Cluster ◽  
Green Bottle

In the present study, the complete mitochondrial genome of the New Zealand parasitic blowfly Lucilia sericata (green bottle blowfly) field strain NZ_LucSer_NP was generated using next-generation sequencing technology. The length of complete the mitochondrial genome is 15,938 bp, with 39.4% A, 13.0% C, 9.3% G, and 38.2% T nucleotide distribution. The complete mitochondrial genome consists of 13 protein-coding genes, two ribosomal RNAs, 22 transfer RNAs, and a and a 1,124 bp non-coding region, similar to most metazoan mitochondrial genomes. Phylogenetic analysis showed that L. sericata NZ_LucSer_NP forms a monophyletic cluster with the remaining six Lucilia species and the Calliphoridae are polyphyletic. This study provides the first complete mitochondrial genome sequence for a L. sericata blowfly species derived from New Zealand to facilitate species identification and phylogenetic analysis.

Complete mitochondrial genome of Xingguo red carp (Cyprinus carpio var. singuonensis) and purse red carp (Cyprinus carpio var. wuyuanensis)

2014 ◽  
Vol 27 (1) ◽  
pp. 244-245 ◽  
Author(s):  
Guang-Fu Hu ◽  
Xiang-Jiang Liu ◽  
Zhong Li ◽  
Hong-Wei Liang ◽  
Shao-Na Hu ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Cyprinus Carpio ◽  
Complete Mitochondrial Genome ◽  
Carp Cyprinus Carpio

scholarly journals The complete mitochondrial genome of Jinbian carp Cyprinus carpio (Cypriniformes: Cyprinidae)

2018 ◽  
Vol 3 (2) ◽  
pp. 1096-1097 ◽  
Author(s):  
Xiangchen Ye ◽  
Yejian Lv ◽  
Lingjing Wei ◽  
Jie Huang ◽  
Yanhong Wen ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Cyprinus Carpio ◽  
Complete Mitochondrial Genome ◽  
Carp Cyprinus Carpio

scholarly journals The complete mitochondrial genome of the common strain black carp (Cyprinus carpio var. baisenensis)

2021 ◽  
Vol 6 (2) ◽  
pp. 542-545
Author(s):  
Peipei Wang ◽  
Opeoluwa Christiana ◽  
Liao Yu ◽  
Chengfeng Zhang ◽  
Shengyan Su ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Cyprinus Carpio ◽  
Complete Mitochondrial Genome ◽  
Black Carp ◽  
Carp Cyprinus Carpio ◽  
The Common

Mitochondrial Genome of Brugia malayi Microfilariae Isolated From a Clinical Sample

2021 ◽  
Vol 9 ◽  
Author(s):  
Xiong Qing ◽  
Kasem Kulkeaw ◽  
Sirichit Wongkamchai ◽  
Stephen Kwok-Wing Tsui
Keyword(s):  
Phylogenetic Analysis ◽  
Mitochondrial Genome ◽  
Dna Sequences ◽  
Gene Annotation ◽  
Clinical Sample ◽  
Complete Mitochondrial Genome ◽  
Trna Genes ◽  
Human Patient ◽  
Close Relationship ◽  
Mt Genome

Lymphatic filariasis is a neglected parasitic disease that is a leading cause of long-term disability. Information obtained from genome sequencing of filarial worm can help us identify systems in the worm that are likely to be useful for novel drug design. Brugia (B.) malayi is still the only lymphatic-dwelling filarial parasite with a nearly complete, fully annotated, and published genome. However, most previous studies were based on the FR3 strain of B. malayi, which originally was isolated from a human patient, and was adapted to the rodent model, then maintained in laboratories for more than 60 years. It is uncertain whether genetic variation exists, thus, sequencing of clinical isolates of lymphatic dwelling filarial parasites is a high priority. Here, we report for the first time the complete mitochondrial genome of B. malayi microfilariae from clinical isolate. Complete mitochondrial (mt) genome of the microfilariae isolated from a blood sample taken from a Thai subject living in Narathiwat Province, which is an endemic area of brugian filariasis, was assembled with sequencing reads obtained by Illumina sequencing. Gene annotation, phylogenetic analysis and single nucleotide polymorphism (SNP) were deployed. A complete 13,658-bp mt genome of B. malayi microfilaria was obtained, and it shows 68x coverage. Based on gene annotation, the mt genome consists of 12 protein-coding, two rRNA, and 23 tRNA genes. Phylogenetic analysis using all protein sequences of DNA sequences of mt genome or cytochrome c oxidase subunit I (COX1) revealed a close relationship among three lymphatic filariae (i.e., B. timori, zoonotic B. pahangi, and Wuchereria spp.). The SNPs in the COX1 gene can differentiate microfilariae of B. malayi in human from those found in canine. Furthermore, the number, order and transcription, and direction of B. malayi microfilariae mitochondrial genes were the same as those found in the FR3 strain of B. malayi. The comparison on mitochondrial genome of B. malayi could have important implications on the development of a new intervention or vaccine to treat or prevent this disease in endemic areas/regions around the world.

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