Identifying the repellent genes in Cannabis (C. sativa) through CRISPR screening. The hidden use of Marijuana

2020 ◽  
Author(s):  
Ambre Lambert ◽  
Shanwa Lee ◽  
Rachel Miller
Keyword(s):  
Cannabis Sativa ◽  
In Vitro Studies ◽  
R Genes ◽  
Root Knot Nematodes ◽  
New Class ◽  
Psychoactive Effects ◽  
Companion Plant ◽  
Chemical Pesticides ◽  
Controversial Nature

Chemical pesticides have caused numerous deaths of people, animals, and plants. As a result, alternative pesticides which are health beneficial and ecological are needed. Cannabis sativa,​ known for its psychoactive effects, can be the solution to this problem. It has excellent repellent characteristics as seen through its use as a companion plant, as well as in-vitro studies. However it has its drawbacks due its controversial nature and lack of research. To solve this problem, our paper aims to locate the non-vital genes in ​C.sativa that cause its repellent effects (R-genes) through CRISPR screening. To optimally identify the R-genes, the random knocked out genes of ​C.sativa were compared to the percentage of alive root-knot nematodes (​M.incognita​) in the plant’s soil. In our experiment, four plants were established per sample: Plant A which is a normal ​Cannabis sativa​, Plant B which is a normal ​Cannabis sativa being infected by ​M.incognita,​ Plant C which is a genetically modified ​Cannabis sativa​, and Plant D which is the same as Plant C except it is being infected by ​M.incognita.​ Then the percentage of alive nematodes will be compared in Plant B and D to identify the R genes. The discovery of R-genes is important as it can be used to discover a new class of repellent molecules. They can also be inserted into crops or household plants, giving them ​Cannabis sativa​’s repellent effects, and benefiting agricultural and health fields. 

scholarly journals Cannabidiol—from Plant to Human Body: A Promising Bioactive Molecule with Multi-Target Effects in Cancer

2019 ◽  
Vol 20 (23) ◽  
pp. 5905 ◽  
Author(s):  
Brigitta Kis ◽  
Feng Chen Ifrim ◽  
Valentina Buda ◽  
Stefana Avram ◽  
Ioana Zinuca Pavel ◽  
...  
Keyword(s):  
Seed Oil ◽  
Clinical Evidence ◽  
Cannabis Sativa ◽  
In Vivo Studies ◽  
Anticancer Effects ◽  
Different Types ◽  
Psychoactive Effects ◽  
Target Effects

Cannabis sativa L. is a plant long used for its textile fibers, seed oil, and oleoresin with medicinal and psychoactive properties. It is the main source of phytocannabinoids, with over 100 compounds detected so far. In recent years, a lot of attention has been given to the main phytochemicals present in Cannabis sativa L., namely, cannabidiol (CBD) and Δ9-tetrahydrocannabinol (THC). Compared to THC, CBD has non-psychoactive effects, an advantage for clinical applications of anti-tumor benefits. The review is designed to provide an update regarding the multi-target effects of CBD in different types of cancer. The main focus is on the latest in vitro and in vivo studies that present data regarding the anti-proliferative, pro-apoptotic, cytotoxic, anti-invasive, anti-antiangiogenic, anti-inflammatory, and immunomodulatory properties of CBD together with their mechanisms of action. The latest clinical evidence of the anticancer effects of CBD is also outlined. Moreover, the main aspects of the pharmacological and toxicological profiles are given.

In vitro - studies with antler bone cells: Structure forming capacity, osteocalcin production and influence of sex steroids

2006 ◽  
Vol 15 (04) ◽  
pp. 245-257 ◽  
Author(s):  
H. J. Rolf ◽  
K. G. Wiese ◽  
H. Siggelkow ◽  
H. Schliephake ◽  
G. A. Bubernik
Keyword(s):  
Sex Steroids ◽  
Bone Cells ◽  
In Vitro Studies ◽  
Osteocalcin Production

In Vitro Activity of Phytocannabinoids from High Potency Cannabis sativa

Planta Medica ◽  
2012 ◽  
Vol 78 (05) ◽  
Author(s):  
A Husni ◽  
S Ross ◽  
O Dale ◽  
C Gemelli ◽  
G Ma ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
High Potency

In Vitro Studies on the Mechanism of the Antifibrino-lytic Action of E-Aminocaproic Acid (EACA)

1968 ◽  
Vol 19 (03/04) ◽  
pp. 584-592 ◽  
Author(s):  
Hanna Lukasiewicz ◽  
S Niewiarowski
Keyword(s):  
Aminocaproic Acid ◽  
Test Tube ◽  
In Vitro Studies ◽  
Lytic Action ◽  
Human Plasminogen ◽  
Experimental Findings ◽  
Fibrin Clots

Summary and Conclusion1. It has been found that EACA does not inhibit activation of human plasminogen into plasmin by SK and UK in a concentration of 5 × 10–2 M. The activation of bovine plasminogen by SK and UK is inhibited by this concentration of EACA but not by a lower one.2. EACA in concentrations of 1,5 × 10–1 – 10–4 M does not inhibit casein proteolysis by plasmin. The proteolysis of fibrinogen and fibrin measured by the release of TCA soluble tyrosine is inhibited by EACA in concentrations of 1,5 × 10–1 – 10–2 M.3. The lysis of non-stabilized clots by plasmin measured in a test tube was inhibited by an EACA concentration of 5 × 10–3 – 5 × 10–4 M. The lysis of stabilized clots by plasmin was inhibited by an EACA concentration of 10–5 M.4. On the basis of experimental findings and data given in literature the authors postulate that the mechanism of the antifibrinolytic effects of EACA consists mainly in a modification of plasmin action on fibrin. These effects are dependent on the structure of the fibrin clots.

The Venom of the Boomslang (Dispholidus Typus): In Vivo and in Vitro Studies

1969 ◽  
Vol 21 (02) ◽  
pp. 234-244 ◽  
Author(s):  
N Mackay ◽  
J.C Ferguson ◽  
Antonia Bagshawe ◽  
A.T.T Forrester ◽  
G.P Mcnicol
Keyword(s):  
In Vitro Studies

SummaryAn account is given of the effects of boomslang venom in man. Evidence was found of a fibrinolytic state apparently secondary to the coagulant action of the venom. These features rapidly responded to the administration of specific antivenom. In vitro studies, using a homogenate of boomslang parotids, confirmed the coagulant properties of the venom and showed them to be of much greater potency than the proteolytic actions.

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