scholarly journals The role of adhesion molecules in bone destruction in rheumatoid arthritis mediated by osteoblastsosteoclasts interaction.

Ensho ◽  
1994 ◽  
Vol 14 (6) ◽  
pp. 467-472
Author(s):  
Masao Shingu ◽  
Michi Hashimoto ◽  
Tetsurou Isayama ◽  
Yasuko Koshihara ◽  
Tatehumi Kuroki ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Adhesion Molecules ◽  
Bone Destruction

The Role of Adhesion Molecules in Synovial Pannus Formation in Rheumatoid Arthritis

1994 ◽  
Vol &NA; (300) ◽  
pp. 297???303
Author(s):  
HITOSHI ISHIKAWA ◽  
SOICHIROU HIRATA ◽  
YASURO NISHIBAYASHI ◽  
SHIGEAKI IMURA ◽  
HITOSHI KUBO ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Adhesion Molecules ◽  
Pannus Formation ◽  
Synovial Pannus

scholarly journals Pharmacokinetics-Based Chronoefficacy of Semen Strychni and Tripterygium Glycoside Tablet Against Rheumatoid Arthritis

2021 ◽  
Vol 12 ◽  
Author(s):  
Jingpan Lin ◽  
Lu Gao ◽  
Yanke Lin ◽  
Shuai Wang ◽  
Zemin Yang ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Bone Destruction ◽  
Systemic Exposure ◽  
Inflammatory Factors ◽  
Systemic Autoimmune Disease ◽  
Active Ingredients ◽  
Tnf Α ◽  
Exposure Levels ◽  
Semen Strychni

Rheumatoid arthritis is a systemic autoimmune disease characterized by synovial inflammation and bone destruction. Identifying drugs with time-varying efficacy and toxicity, and elucidating the mechanisms would help to improve treatment efficacy and reduce adverse effects. Here, we aimed to determine the chronoefficacy of semen strychni (SS) and tripterygium glycoside tablet (TGT) against rheumatoid arthritis in mice, and to investigate a potential role of circadian pharmacokinetics in generating chronoefficacy. SS extract and TGT suspension were prepared with ultrasonication. Effects of SS and TGT on collagen-induced arthritis (CIA) were evaluated by measuring TNF-α and IL-6 levels. SS dosed at ZT18 was more effective in protecting against CIA than drug dosed at ZT6 (i.e., lower levels of key inflammatory factors at ZT18 than at ZT6). This was accompanied by higher systemic exposure levels of strychnine and brucine (two main putative active ingredients of SS) in ZT18-treated than in ZT6-treated CIA mice. TGT dosing at ZT2 showed a better efficacy against CIA as compared to herb doing at ZT14. Consistently, ZT2 dosing generated a higher exposure of triptolide (a main putative active ingredient of TGT) as compared to ZT14 dosing in CIA mice. Moreover, strychnine, brucine, and triptolide significantly inhibited the proliferation of fibroblast-like synoviocytes, and reduced the production of TNF-α and IL-6 and the mRNAs of TNF-α, IL-6, COX-2, and iNOS, suggesting that they possessed an anti-arthritis activity. In conclusion, SS and TGT display chronoefficacy against rheumatoid arthritis in mice, that is attributed to circadian pharmacokinetics of main active ingredients. Our findings have implications for improving treatment outcomes of SS and TGT via timed delivery.

TLRs Play Crucial Roles in Regulating RA Synoviocyte

2020 ◽  
Vol 20 (8) ◽  
pp. 1156-1165 ◽  
Author(s):  
Xuling Luo ◽  
Juncheng Cui ◽  
Xin Long ◽  
Zhiwei Chen
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cell ◽  
Inflammatory Disease ◽  
Chinese Herb ◽  
Bone Destruction ◽  
Inflammatory Factors ◽  
Exact Role ◽  
Autoimmune Inflammatory Disease ◽  
Fibroblast Like Synoviocytes

Rheumatoid arthritis (RA) is an autoimmune inflammatory disease comparing the inflammation of synovium. Macrophage-like synoviocytes and fibroblast-like synoviocytes (synoviocytes) are crucial ingredients of synovium. Therein, a lot of research has focused on synoviocytes. Researches demonstrated that TLR1, TLR2, TLR3, TLR4, TLR5, TLR6 TLR7 and TLR9 are expressed in synoviocyte. Additionally, the expression of TLR2, TLR3, TLR4 and TLR5 is increased in RA synoviocyte. In this paper, we review the exact role of TLR2, TLR3, TLR4 and TLR5 participate in regulating the production of inflammatory factors in RA synoviocyte. Furthermore, we discuss the role of vasoactive intestinal peptide (VIP), MicroRNA, Monome of Chinese herb and other cells (Monocyte and T cell) influence the function of synoviocyte by regulating TLRs. The activation of toll-like receptors (TLRs) in synoviocyte leads to the aggravation of arthritis, comparing with angiogenesis and bone destruction. Above all, TLRs are promising targets for managing RA.

scholarly journals Bone destruction in rheumatoid arthritis: role of autoantibodies

2013 ◽  
Vol 0 (3) ◽  
pp. 267
Author(s):  
Anastasia Sergeyevna Avdeyeva ◽  
E N Aleksandrova ◽  
A A Novikov ◽  
A V Smirnov ◽  
M V Cherkasova ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Bone Destruction

scholarly journals Multi-omics Analysis of the Biomarkers and Molecular Mechanism of Rheumatoid Arthritis With Bone Destruction

2021 ◽  
Author(s):  
Qian Huang ◽  
Jiang Su ◽  
Weihua Zhang ◽  
Shengjia Chang ◽  
Silin Li ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
16S Rrna ◽  
Bone Destruction ◽  
Gut Bacteria ◽  
Plasma Metabolites ◽  
Healthy Control ◽  
Novel Biomarkers ◽  
Bacteria And Fungi ◽  
And Function

Abstract ObjectivesOur study aimed to elucidate the role of metabolites, bacteria, and fungi in rheumatoid arthritis (RA) patients with bone destruction (BD(+)) and find some biomarkers to predicate bone progression of RA.MethodsWe conducted plasma metabolites of the 127 RA patients and 69 healthy control by using nontargeted liquid chromatography-mass spectrometry (LC-MS), and the gut bacteria and fungi were assessed by 16S rRNA and internal transcribed spacer (ITS).ResultsCompared with RA patients without bone destruction (BD(-)), some metabolites, bacteria, and fungi altered in BD(+). Sever metabolites were selected as key metabolites for classifying the BD(+) and BD(-) groups with moderate accuracy (AUC=0.71). Metabolites-groups, metabolites-metabolites, and metabolites-clinical factors had a certain correlation, and 7 metabolites were enriched in glycerophospholipid metabolism and L-arginine and proline metabolism pathways. The bacteria and fungi of the BD(+) group showed significant differences in composition and function compared with BD(-) group. The changed 4 bacteria and 12 fungi yielded accuracy (AUC=0.74 and AUC=0.87, respectively) for the two groups. Taken 7 metabolites, 4 bacteria and 12 fungi as a panel for AUC analysis, an improved AUC of 0.99 significantly discriminated the two groups. The changed metabolites, gut bacteria, and fungi may affected the pathway related to L-arginine.ConclusionsOur nontargeted LC-MS, 16S rRNA, and ITS highlight a novel link among the metabolites, bacteria, fungi, and pathology of BD(+), which contributed to our understanding of the role of metabolites, bacteria, and fungi in BD(+) aetiology and offers some novel biomarkers to predict the bone progression of RA.

scholarly journals Implication of the Association of Fibrinogen Citrullination and Osteoclastogenesis in Bone Destruction in Rheumatoid Arthritis

Cells ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 2720
Author(s):  
Ji Soo Kim ◽  
Mikyung Choi ◽  
Ji Yong Choi ◽  
Joo Yeon Kim ◽  
Jeong Yeon Kim ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Bone Erosion ◽  
Bone Destruction ◽  
Peptidylarginine Deiminase ◽  
Inhibitory Function ◽  
Spectral Peaks ◽  
Polymerase Chain ◽  
Gene Expression Levels

Immune complexes containing citrullinated fibrinogen are present in the sera and synovium of rheumatoid arthritis patients and potentially contribute to synovitis. However, fibrinogen can inhibit the osteoclastogenesis of precursor cells. We investigated the direct effect of citrullinated fibrinogen on osteoclastogenesis to understand the role of citrullination on bone erosion of rheumatoid arthritis patients. We evaluated the fibrinogen citrullination sites using mass spectrometry and quantified osteoclast-related protein and gene expression levels by Western blotting, microarray, and real-time polymerase chain reaction. Differences in spectral peaks were noted between fibrinogen and citrullinated fibrinogen at five sites in α-chains, two sites in β-chains, and one site in a γ-chain. Transcriptome changes induced by fibrinogen and citrullinated fibrinogen were identified and differentially expressed genes grouped into three distinctive modules. Fibrinogen was then citrullinated in vitro using peptidylarginine deiminase. When increasing doses of soluble fibrinogen and citrullinated fibrinogen were applied to human CD14+ monocytes, citrullination restored osteoclastogenesis-associated changes, including NF-ATc1 and ß3-integrin. Finally, citrullination rescued the number of osteoclasts by restoring fibrinogen-induced suppression of osteoclastogenesis. Taken together, the results indicate that the inhibitory function of fibrinogen on osteoclastogenesis is reversed by citrullination and suggest that citrullinated fibrinogen may contribute to erosive bone destruction in rheumatoid arthritis.

Sign in / Sign up

Export Citation Format

Share Document