scholarly journals Electron-Beam Excitation Assisted (EXA) Optical Microscopy for High Resolution Imaging

2020 ◽  
Vol 86 (7) ◽  
pp. 515-519
Author(s):  
Yoshimasa KAWATA
Keyword(s):  
Electron Beam ◽  
High Resolution ◽  
Optical Microscopy ◽  
High Resolution Imaging ◽  
Electron Beam Excitation ◽  
Resolution Imaging ◽  
Beam Excitation

scholarly journals High-resolution, label-free imaging of living cells with direct electron-beam-excitation-assisted optical microscopy

2015 ◽  
Vol 23 (11) ◽  
pp. 14561 ◽  
Author(s):  
Yasunori Nawa ◽  
Wataru Inami ◽  
Sheng Lin ◽  
Yoshimasa Kawata ◽  
Susumu Terakawa
Keyword(s):  
Electron Beam ◽  
High Resolution ◽  
Optical Microscopy ◽  
Living Cells ◽  
Label Free ◽  
Electron Beam Excitation ◽  
Beam Excitation

The method of replication affects metal film granularity and resolution

1989 ◽  
Vol 47 ◽  
pp. 708-709
Author(s):  
George C. Ruben
Keyword(s):  
Electron Beam ◽  
High Resolution ◽  
Film Thickness ◽  
Single Molecule ◽  
Metal Film ◽  
Vertical Surface ◽  
High Resolution Imaging ◽  
Controllable Factors ◽  
Resolution Imaging

Single molecule resolution in electron beam sensitive, uncoated, noncrystalline materials has been impossible except in thin Pt-C replicas ≤ 150Å) which are resistant to the electron beam destruction. Previously the granularity of metal film replicas limited their resolution to ≥ 20Å. This paper demonstrates that Pt-C film granularity and resolution are a function of the method of replication and other controllable factors. Low angle 20° rotary , 45° unidirectional and vertical 9.7±1 Å Pt-C films deposited on mica under the same conditions were compared in Fig. 1. Vertical replication had a 5A granularity (Fig. 1c), the highest resolution (table), and coated the whole surface. 45° replication had a 9Å granulartiy (Fig. 1b), a slightly poorer resolution (table) and did not coat the whole surface. 20° rotary replication was unsuitable for high resolution imaging with 20-25Å granularity (Fig. 1a) and resolution 2-3 times poorer (table). Resolution is defined here as the greatest distance for which the metal coat on two opposing faces just grow together, that is, two times the apparent film thickness on a single vertical surface.

scholarly journals Diffraction Microscopy using 20-kV Electron Beam for Multi-Wall Carbon Nanotubes

2007 ◽  
Vol 1026 ◽  
Author(s):  
Osamu Kamimura ◽  
Kota Kawahara ◽  
Takahisa Doi ◽  
Takashi Dobashi ◽  
Takashi Abe ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Electron Beam ◽  
High Resolution ◽  
Low Energy ◽  
High Resolution Imaging ◽  
Diffractive Imaging ◽  
Characteristic Structure ◽  
Multi Wall Carbon Nanotubes ◽  
Resolution Imaging ◽  
Diffraction Microscopy

AbstractDiffraction microscopy (or diffractive imaging) with iterative phase retrieval was performed using a low-energy (20-keV) electron beam to verify the possibility of high-resolution imaging with low specimen damage. Diffraction patterns of fine and uniform multi-wall carbon nanotubes (MWCNT) were recorded without a post-specimen lens. One- and two-dimensional phase retrievals were processed from the diffraction pattern alone. The reconstructed object images reflected the characteristic structure of the MWCNT. These results show the possibility of high-resolution imaging with a low-energy electron beam.

High resolution fluorescent bio-imaging with electron beam excitation

Microscopy ◽  
2014 ◽  
Vol 63 (suppl 1) ◽  
pp. i16-i16 ◽  
Author(s):  
Yoshimasa Kawata ◽  
Yasunori Nawa ◽  
Wataru Inami
Keyword(s):  
Electron Beam ◽  
High Resolution ◽  
Electron Beam Excitation ◽  
Bio Imaging ◽  
Beam Excitation

scholarly journals Dynamic and high-resolution live cell imaging by direct electron beam excitation

2012 ◽  
Vol 20 (5) ◽  
pp. 5629 ◽  
Author(s):  
Yasunori Nawa ◽  
Wataru Inami ◽  
Akito Chiba ◽  
Atsushi Ono ◽  
Atsuo Miyakawa ◽  
...  
Keyword(s):  
Electron Beam ◽  
High Resolution ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Electron Beam Excitation ◽  
Beam Excitation
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