In vivo and in vitro Antidiabetic Characterization of Nymphaea alba leaves

International Journal of Pharmaceutical Sciences and Drug Research ◽

10.25004/ijpsdr.2020.120520 ◽

2020 ◽

pp. 565-571

Author(s):

GURVIRENDER SINGH ◽

SUPRIYA AGNIHOTRI ◽

SANTOSH KUMAR VERMA

Keyword(s):

Methanol Extract ◽

In Vitro Study ◽

Diabetic Rats ◽

Inhibition Assay ◽

Maximum Inhibition ◽

Nymphaea Alba

The study was aimed to trace out antihyperglycemic potentials of Nymphaea alba leaves using in vitro and in vivo approaches. In, in vitro study, determination of IC50 of Nymphaea alba extracts was done using α-amylase and α-glucosidase inhibition assay. In α-amylase, Nymphaea alba methanol extract (NAME) exhibited maximum inhibition 56.77±1.23% at 125µg/ml in comparison to 66.7±0.94 % of standard acarbose. In α-glucosidase, NAME exhibits 59.89±0.92, while standard acarbose showed 70.31±1.25 % inhibition. The in vivo study was performed on diabetic rats, made diabetic by Streptozotocin. 200mg/kg and 400mg/kg NAME was administered orally, which significantly (

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Protective effect of atorvastatin meditated by HMGCR gene on diabetic rats with atherosclerosis: An in vivo and in vitro study

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2018.04.179 ◽

2018 ◽

Vol 104 ◽

pp. 240-251 ◽

Cited By ~ 3

Author(s):

Yong-Zhi Wang ◽

Lei Yang ◽

Chuan-Fang Li

Keyword(s):

Protective Effect ◽

In Vitro Study ◽

Diabetic Rats ◽

Vitro Study

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In vivo and in vitro trimethadione oxidation activity of the liver from various animal species including mouse, hamster, rat, rabbit, dog, monkey and human

Human & Experimental Toxicology ◽

10.1177/096032719901800102 ◽

1999 ◽

Vol 18 (1) ◽

pp. 12-16 ◽

Cited By ~ 2

Author(s):

E Tanaka ◽

A Ishikawa ◽

T Horie

Keyword(s):

Animal Species ◽

In Vitro Study ◽

Metabolic Clearance ◽

Oxidation Activity ◽

In Vitro Characterization ◽

Demethylase Activity ◽

Total Body

Trimethadione (TMO) has the properties required of a probe drug for the evaluation of hepatic drug-oxidizing capacity and, in this study, we have summarized the in vivo and in vitro metabolism of TMO in various animal species including mouse, hamster, rat, rabbit, dog, monkey and human. In the in vivo study, the plasma TMO level was measured after intravenous or oral (human) administration of TMO at a dose of 4 mg/kg to various animal species. The rate of TMO metabolic clearance in these animal species in vivo was in the order mouse > hamster >rat>rabbit>dog>monkey>human. In the in vitro study, species differences were observed in the cytochrome P450 (P450) content and drug-oxidizing enzyme activity. The content of P450 was monkey> mouse>dog>rabbit>hamster>rat>human. On the other hand, TMO N-demethylation was in the order mouse >hamster >rat >rabbit>dog>monkey>human. There was a good correlation between the mean total body clearance of TMO ( in vivo)andthemeanTMON-demethylase activity ( in vitro) (y=1.7×+0.11, r=0.965, P<0.001). These results show that TMO is a probe agent with metabolic and pharmacokinetic characteristics making it attractive for the in vivo and in vitro characterization of metabolic activity in various animal species.

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Characterization of a New Chitosanase from a Marine Bacillus sp. and the Anti-Oxidant Activity of Its Hydrolysate

Marine Drugs ◽

10.3390/md18020126 ◽

2020 ◽

Vol 18 (2) ◽

pp. 126

Author(s):

Chunrui Ma ◽

Xiao Li ◽

Kun Yang ◽

Shangyong Li

Keyword(s):

Radical Scavenging ◽

Maximal Activity ◽

Hydroxyl Groups ◽

Bacillus Sp ◽

Low Molecular Weight Chitosan ◽

Anti Oxidant

Chitooligosaccharide (COS) has been recognized to exhibit efficient anti-oxidant activity. Enzymatic hydrolysis using chitosanases can retain all the amino and hydroxyl groups of chitosan, which are necessary for its activity. In this study, a new chitosanase encoding gene, csnQ, was cloned from the marine Bacillus sp. Q1098 and expressed in Escherichia coli. The recombinant chitosanase, CsnQ, showed maximal activity at pH 5.31 and 60 °C. Determination of CsnQ pH-stability showed that CsnQ could retain more than 50% of its activity over a wide pH, from 3.60 to 9.80. CsnQ is an endo-type chitosanase, yielding chitodisaccharide as the main product. Additionally, in vitro and in vivo analyses indicated that chitodisaccharide possesses much more effective anti-oxidant activity than glucosamine and low molecular weight chitosan (LMW-CS) (~5 kDa). Notably, to our knowledge, this is the first evidence that chitodisaccharide is the minimal COS fragment required for free radical scavenging.

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Characterization of non-lymphoid cells in the white pulp of the mouse spleen: An in vivo and in vitro study

Cell and Tissue Research ◽

10.1007/bf00233888 ◽

1978 ◽

Vol 195 (3) ◽

Cited By ~ 36

Author(s):

P. Eikelenboom

Keyword(s):

In Vitro Study ◽

Lymphoid Cells ◽

Vitro Study ◽

Mouse Spleen ◽

White Pulp

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Antibacterial Activity of Buah Merah (Pandanus conoideus Lam.) Against Bacterial Oral Pathogen of Streptococcus sanguinis ATCC10556, Streptococcus mutans ATCC 25175, and Enterococcus faecalis ATCC 29212: An in Vitro Study

The Open Dentistry Journal ◽

10.2174/18742106020140113 ◽

2020 ◽

Vol 14 (1) ◽

pp. 113-119 ◽

Cited By ~ 1

Author(s):

Lisda Damayanti ◽

Ida Ayu Evaangelina ◽

Avi Laviana ◽

Yetty Herdiyati ◽

Dikdik Kurnia

Keyword(s):

Antibacterial Activity ◽

Ethyl Acetate ◽

Methanol Extract ◽

Synergistic Effects ◽

In Vitro Study ◽

Inhibition Zone ◽

Oral Bacteria ◽

Inhibition Zones

Background: Caries and periodontitis are dental diseases caused by bacteria of S. sanguinis, S. mutans, and E. faecalis with three main etiological factors of the host, substrate, and time. Objective: This study proposed to investigate the antibacterial effects of Buah Merah (Pandanus conoideus Lam.) against oral bacteria of E.faecalis, S. mutans, and S. sanguinis. Materials and Methods: The Buah Merah was extracted with different solvents to yield n-hexane, ethyl acetate, methanol, and H2O extracts. The concentrations of single and mixture extracts were adjusted for antibacterial assay against bacteria of E. faecalis, S. mutans, and S. sanguinis strains through agar well diffusion assay with chlorhexidine, fosfomycin, and quercetin used as positive controls. Results: The ethyl acetate extract showed highest antibacterial activity against three oral bacterial of E. faecalis, S. mutans, and S. sanguinis with inhibition zones values of 9.3, 12.3, and 17.9 mm at 40%, respectively, together with their MIC and MBC values of 1250 & 2500, 0.312 & 0.625, and 0.312 & 0.625 ppm, respectively. For the formulation of extracts, combinations samples test gave various effects to different bacteria, with the best activity showed by methanol-ethyl acetate (M-Ea) extracts against S. mutans with an inhibition zone of 16.25 mm at 40 ppm. The strong and synergistic effect of methanol extract against S. mutans was supported by inhibition zones of the formulation of methanol extract-fosfomycin which showed an inhibition zone of 25.9 mm at 10 ppm. Conclusion: The extracts of Buah Merah demonstrated antibacterial activity against oral bacteria of E. faecalis, S. mutans, and S. sanguinis and gave important information for further in vivo clinical studies to determine the exact dosages and its effectiveness in practical application. These results prove the antimicrobial effects of Buah Merah extracts as alternative natural drugs with synergistic effects of active constituents.

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Antioxidant, anti-inflammatory and hypoglycemic effects of Fagonia olivieri DC on STZ-nicotinamide induced diabetic rats - In vivo and in vitro study

Journal of Ethnopharmacology ◽

10.1016/j.jep.2019.112038 ◽

2019 ◽

Vol 242 ◽

pp. 112038 ◽

Cited By ~ 7

Author(s):

Umbreen Rashid ◽

Muhammad Rashid Khan ◽

Moniba Sajid

Keyword(s):

In Vitro Study ◽

Diabetic Rats ◽

Vitro Study ◽

Anti Inflammatory

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Mitochondrial Neurogastrointestinal Encephalomyopathy (MNGIE): Biochemical Features and Therapeutic Approaches

Bioscience Reports ◽

10.1007/s10540-007-9043-2 ◽

2007 ◽

Vol 27 (1-3) ◽

pp. 151-163 ◽

Cited By ~ 43

Author(s):

M. C. Lara ◽

M. L. Valentino ◽

J. Torres-Torronteras ◽

M. Hirano ◽

R. Martí

Keyword(s):

Molecular Genetic ◽

In Vivo Studies ◽

Gene Encoding ◽

Mitochondrial Neurogastrointestinal Encephalomyopathy ◽

Mtdna Replication ◽

Biochemical Features

Over the last 15 years, important research has expanded our knowledge of the clinical, molecular genetic, and biochemical features of mitochondrial neurogastrointestinal encephalomyopathy (MNGIE). The characterization of mitochondrial involvement in this disorder and the seminal determination of its genetic cause, have opened new possibilities for more detailed and deeper studies on the pathomechanisms in this progressive and fatal disease. It has been established that MNGIE is caused by mutations in the gene encoding thymidine phosphorylase (TP), which lead to absolute or nearly complete loss of its catalytic activity, producing systemic accumulations of its substrates, thymidine (dThd) and deoxyuridine (dUrd). Findings obtained from in vitro and in vivo studies indicate that the biochemical imbalances specifically impair mitochondrial DNA (mtDNA) replication, repair, or both leading to mitochondrial dysfunction. We have proposed that therapy for MNGIE should be aimed at reducing the concentrations of these toxic nucleosides to normal or nearly normal levels. The first treatment, allogeneic stem-cell transplantation (alloSCT) reported in 2006, produced a nearly full biochemical correction of the dThd and dUrd imbalances in blood. Clinical follow-up of this and other patients receiving alloSCT is necessary to determine whether this and other therapies based on a permanent restoration of TP will be effective treatment for MNGIE.

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Development, in vitro and in vivo evaluations of novel lipid drug delivery system of Newbouldia laevis (P. Beauv.)

Nanobiomedicine ◽

10.1177/1849543516673445 ◽

2016 ◽

Vol 3 ◽

pp. 184954351667344

Author(s):

Chukwuebuka Umeyor ◽

Emmanuel Anaka ◽

Franklin Kenechukwu ◽

Chinazom Agbo ◽

Anthony Attama

Keyword(s):

Encapsulation Efficiency ◽

Folk Medicine ◽

Research Work ◽

Lipid Matrix ◽

Solid Lipid ◽

Development In Vitro

Newbouldia laevis (P. Beauv.) is a tropical rainforest plant used in traditional folk medicine for the treatment of malaria, cough, joint pains, stomach ache, oedema and inflammation. The main thrust of this research work was to study the analgesic/anti-nociceptive properties of N. laevis-loaded solid lipid microdispersions. N. laevis leaves were extracted using ethanol, and the extract was formulated into solid lipid microdispersions using lipid matrix comprising a rational blend of Precirol® ATO 5 and Softisan® 154. Characterization of the solid lipid microdispersions include determination of morphology, particle size, pH, thermal property, encapsulation efficiency percentage and analgesic/anti-nociceptive property. The results obtained showed that the particles were spherical with sizes ranging from 40 µm to 125 µm. The solid lipid microdispersions maintained a stable pH within the acidic region of 5–6 with insignificant variations ( p > 0.05) over a period of 90 days. Thermal analysis showed that N. laevis was entrapped in the lipid matrix used for the formulations. Solid lipid microdispersions recorded a maximum encapsulation efficiency up to 88.1%. N. laevis-loaded solid lipid microdispersions also produced good analgesic/anti-nociceptive property comparable with the standard diclofenac potassium. N. laevis-loaded solid lipid microdispersions showed good analgesic/anti-nociceptive effect and could be used in the treatment and management of pain.

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Antidiabetic‎ Activity of Terfezia Claveryi; An In Vitro and In Vivo Study

Biomedical & Pharmacology Journal ◽

10.13005/bpj/1680 ◽

2019 ◽

Vol 12 (2) ◽

pp. 603-608

Author(s):

Anas AlAhmed ◽

Hany Ezzat Khalil

Keyword(s):

Methanol Extract ◽

Positive Control ◽

Alpha Tocopherol ◽

Antidiabetic Activity ◽

Inhibition Assay ◽

Ic50 Value ◽

Enzymatic Glycosylation ◽

Terfezia Claveryi

The main objective of current study was to investigate the in vitro and in vivo antidiabetic activity of Terfezia claveryi methanol extract. In vitro antidiabetic assays such as inhibition of α-amylase enzyme and non-enzymatic glycosylation of hemoglobin were carried out. The results of α- amylase inhibition assay revealed that the inhibitory activity (IC50) of Terfezia claveryi methanol extract (‎38.7µg/ml) is stronger when compared with positive control (Acarbose IC50 value of ‎45.3‎ µg/ml). The inhibition of glycosylation of hemoglobin of Terfezia claveryi methanol extract showed almost the same IC50 (33.1µg/ml) when compared the positive control, alpha-tocopherol (‎35.4µg/ml‎). In vivo antidiabetic study revealed that Terfezia claveryi methanol extract ‎ possessed good activity at a dose of 200 mg/kg through reducing the fasting plasma glucose level (122.1‎±‎3.0 mg/dl) when compared with positive control (Glibenclamide of ‎79.4±1.4‎ mg/dl) (p < 0.001). The results from this study indicated that Terfezia claveryi methanol extract exhibited considerable in vitro and in vivo antidiabetic activities. These possible activities could be useful to consider Terfezia claveryi ‎ as therapeutic antidiabetic candidate.

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