Terazosin treatment suppresses basic fibroblast growth factor expression in the rat ventral prostate

Purpose: Alpha1-adrenergic receptor antagonists may not act solely on smooth muscle contractility. We evaluated the in vivo effect of the alpha1 blocker, terazosin, on the expression of basic fibroblast growth factor (bFGF) in the rat ventral prostate. Methods: Wistar rats were treated with terazosin (1.2 mg/kg body weight, po, every second day) for 120 days. The expression of bFGF was assessed immuno-histochemically in tissue sections and by Western blotting in whole tissue preparations. Results: Terazosin treatment did not affect prostate weight or histomorphology. In the control group, epithelial and stromal cells demonstrated positive staining for the anti-bFGF antibody. In contrast, the same staining in terazosin-treated specimens was either absent or extremely weak. An analogous difference was observed among the corresponding immunoblots. Conclusions: These findings implicate the reduction of bFGF expression by terazosin as a potential additional molecular mechanism of its action that may include alterations in peptide growth factor mediated prostate homeostasis.

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TERAZOSIN TREATMENT SUPPRESSES BASIC FIBROBLAST GROWTH FACTOR EXPRESSION IN THE RAT VENTRAL PROSTATE

The Journal of Urology ◽

10.1097/00005392-199904020-00441 ◽

1999 ◽

pp. 360

Author(s):

Dionisios Mitropoulos ◽

Aspasia Kiroudi ◽

Anastasios Zervas ◽

Nikolaos Antoniou ◽

Christos Kittas ◽

...

Keyword(s):

Growth Factor ◽

Fibroblast Growth Factor ◽

Basic Fibroblast Growth Factor ◽

Ventral Prostate ◽

Fibroblast Growth ◽

Rat Ventral Prostate ◽

Factor Expression ◽

Growth Factor Expression

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Evaluation of the bioactivity about anti-sca-1/basic fibroblast growth factor-urinary bladder matrix scaffold for pelvic reconstruction

Journal of Biomaterials Applications ◽

10.1177/0885328218811390 ◽

2018 ◽

Vol 33 (6) ◽

pp. 808-818 ◽

Cited By ~ 2

Author(s):

Jiankui Li ◽

Xi Chen ◽

Kaijian Ling ◽

Zhiqing Liang ◽

Huicheng Xu

Keyword(s):

Growth Factor ◽

Urinary Bladder ◽

Fibroblast Growth Factor ◽

Basic Fibroblast Growth Factor ◽

Pelvic Organ ◽

Fibroblast Growth ◽

Pelvic Reconstruction ◽

Urinary Bladder Matrix

Introduction and hypothesis: Pelvic support structure injury is the major cause of pelvic organ prolapse. At present, polypropylene-based filler material has been suggested as a common method to treat pelvic organ prolapse. However, it cannot functionally rehabilitate the pelvic support structure. In addition to its poor long-term efficiency, the urinary bladder matrix was the most suitable biological scaffold material for pelvic floor repair. Here, we hypothesize that anti-sca-1 monoclonal antibody and basic fibroblast growth factor were cross-linked to urinary bladder matrix to construct a two-factor bioscaffold for pelvic reconstruction. Methods Through a bispecific cross-linking reagent, sulfosuccinimidyl 4-[N-maleimidomethyl] cyclohexane-1-carboxylate (sulfo-smcc) immobilized anti-sca-1 and basic fibroblast growth factor to urinary bladder matrix. Then scanning electron microscope and plate reader were used to detect whether the anti-sca-1/basic fibroblast growth factor-urinary bladder matrix scaffold was built successfully. After that, the capacity of enriching sca-1 positive cells was measured both in vitro and in vivo. In addition, we evaluated the differentiation capacity and biocompatibility of the scaffold. Finally, western blotting was used to detect the level of fibulin-5 protein. Results The scanning electron microscope and plate reader revealed that the double-factor biological scaffold was built successfully. The scaffold could significantly enrich a large number of sca-1 positive cells both in vitro and in vivo, and obviously accelerate cells and differentiate functional tissue with good biocompatibility. Moreover, the western blotting showed that the scaffold could improve the expression of fibulin-5 protein. Conclusion The anti-sca-1/basic fibroblast growth factor-urinary bladder matrix scaffold revealed good biological properties and might serve as an ideal scaffold for pelvic reconstruction.

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