scholarly journals Simultaneous Quantification of Hederacoside C and α-hederin in Hedera Nepalensis K.Koch Using HPLC-UV

2020 ◽  
Vol 36 (3) ◽  
Author(s):  
Do Thi Thuy Linh ◽  
Hoang Thanh Duong ◽  
Nguyen Tuan Hiep ◽  
Pham Thanh Huyen ◽  
Nguyen Minh Khoi ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Rapid Determination ◽  
Publishing House ◽  
Leaf Extracts ◽  
Hedera Helix ◽  
Dipeptidyl Peptidase 4 ◽  
Simultaneous Quantification ◽  
Study Results

 This study develops a high performance liquid chromatography with ultraviolet detection (HPLC-UV) for simultaneous quantification of hederacoside C and α-hederin in Hedera nepalensis K. Koch. The method proposed in this study was validated in terms of the analytical parameters such as high repeatability, high accuracy and good sensitivity. The method was used to determine the content of hederacoside C and α-hederin in Hedera nepalensis K. Koch, which had been collected in Ha Giang, Lao Cai and Lai Chau. The study results show that the content of hederacoside C and the content of α-hederin ranged from 0.40 to 4.01% and 0.21 – 0.54% based on absolute dry mass, respectively. Keywords Hedera nepalensis K. Koch, hederacoside C, α-hederin, HPLC-UV. References [1] L. Jafri, et al, In vitro assessment of antioxidant potential and determination of polyphenolic compounds of Hedera nepalensis K. Koch, Arabian Journal of Chemistry. 10 (2017) 3699-3706. https://doi.org/10.1016/j.arabjc.2014.05.002.[2] S. Saleem, et al, Plants Fagonia cretica L, and Hedera nepalensis K. Koch contain natural compounds with potent dipeptidyl peptidase-4 (DPP-4) inhibitory activity, Journal of ethnopharmacology. 156 (2014) 26-32. https://doi.org/10.1016/j.jep.2014.08.017[3] D.H. Bich, Medicinal plants and animals for medicine in Vietnam, Vol 1, Science and Technics Publishing House, Hanoi, 2006 (in Vietnamese).[4] National Institute Of Medicinal Materials, List of medicinal plants in Vietnam, Science and Technics Publishing House, Hanoi, 2016 (in Vietnamese).[5] L. Jafri, et al, Hedera nepalensis K. Koch: A Novel Source of Natural Cancer Chemopreventive and Anticancerous Compounds, Phytotherapy research. 30(3) (2016) 447-453. https://doi.org/10.1002/ptr.5546. [6] S. Kanwal, et al, Antioxidant, antitumor activities and phytochemical investigation of Hedera nepalensis K. Koch, an important medicinal plant from Pakistan, Pakistan Journal of Botany. 43 (2011) 85-89. [7] G. Uddin, et al, Biological screening of ethyl acetate extract of Hedera nepalensis stem, African Journal of Pharmacy and Pharmacology. 6(42) (2012) 2934-2937. https://doi.org/10.5897/AJPP12.828 [8] H. Kizu, et al, Studies on Nepalese Crude Drugs, III, On the Saponins of Hedera nepalensis K. Koch, Chemical and Pharmaceutical Bulletin. 33(8) (1985) 3324-3329. https://doi.org/0.1248/cpb.33.3324[9] X. Tong, et al, Extraction and GC-MS Analysis of Volatile Oil from Hedera nepalensis var sinensis, Fine Chemicals. 24(6) (2007) 559-561. [10] EDQM, European Pharmacopoeia, fifth ed., Council of Europe, France, 2015. [11] N.T.H. Mai, et al, Simultaneous Quantification of Hederacoside C and α-Hederin from the Leaves of Hedera helix L. by HPLC, Journal of Medicinal Material. 21(6) (2016). (in Vietnamese).[12] L. Havlíková, et al, Rapid Determination of α-Hederin and Hederacoside C in Extracts of Hedera helix Leaves Available in the Czech Republic and Poland, Natural product communications. 10(9) (2015). https://doi.org/10.1177/1934578X1501000910[13] M. Yu, et al, Determination of Saponins and Flavonoids in Ivy Leaf Extracts Using HPLC-DAD, Journal of Chromatographic Science. 53(4) (2014) 478-483. https://doi.org/10.1093/chromsci/bmu068.[14] EMEA, Validation of analytical procedures: text and methodology Q2 (R1), in International conference on harmonization, Geneva, Switzerland, 2005. [15] W. Horwitz, Official methods of analysis, 12 ed., Vol 1, Association of Official Analytical Chemists, Washington DC, 1975.

scholarly journals Rapid Determination of α-Hederin and Hederacoside C in Extracts of Hedera helix Leaves Available in the Czech Republic and Poland

2015 ◽  
Vol 10 (9) ◽  
pp. 1934578X1501000
Author(s):  
Lucie Havlíková ◽  
Kateřina Macáková ◽  
Lubomír Opletal ◽  
Petr Solich
Keyword(s):  
Mobile Phase ◽  
Ammonium Acetate ◽  
Rapid Determination ◽  
Hplc Method ◽  
Leaf Extracts ◽  
Hedera Helix ◽  
The Czech Republic ◽  
Upper Respiratory ◽  
Isocratic Mode

Leaf extracts of Hedera helix L. are widely used in the treatment of upper respiratory diseases. The saponins α-hederin and hederacoside C are considered to be the main compounds responsible for the biological activity. α-Hederin and hederacoside C were determined in H. helix leaf extracts using a fast, simple and validated HPLC method. An XTerra MS C18 column and mobile phase composed of 10 mM ammonium acetate at pH 8.5 (adjusted with triethylamine) and acetonitrile were used for the chromatography at 1.2 mL min−1. The column was kept at 30°C. Detection was performed at 220 nm. An approach utilizing a basic pH of the aqueous part of the mobile phase enabled analysis in 5 minutes in isocratic mode. The method was validated and used for the quality control of H. helix leaf ethanolic extracts.

scholarly journals In vitro determination of the anti-aging potential of four southern African medicinal plants

2013 ◽  
Vol 13 (1) ◽  
Author(s):  
Gugulethu Ndlovu ◽  
Gerda Fouche ◽  
Malefa Tselanyane ◽  
Werner Cordier ◽  
Vanessa Steenkamp
Keyword(s):  
Medicinal Plants

The interferon-induced enzyme oligo-isoadenylate synthetase: Rapid determination of its in vitro products

1981 ◽  
Vol 110 (1) ◽  
pp. 190-196 ◽  
Author(s):  
Gilles Merlin ◽  
Michel Revel ◽  
David Wallach
Keyword(s):  
Rapid Determination

scholarly journals Antimycotic Activity of Leaf Extracts of Medicinal Plants Against Dermatophytes

2020 ◽  
Vol 1 (1) ◽  
pp. 33-40
Author(s):  
Renu Jangid ◽  
Tahira vbegum
Keyword(s):  
Medicinal Plants ◽  
Diethyl Ether ◽  
Trichophyton Mentagrophytes ◽  
Ziziphus Mauritiana ◽  
Moderate Activity ◽  
Microsporum Gypseum ◽  
Leaf Extracts ◽  
Plant Materials ◽  
Antimycotic Activity

Plant products have been used as medicines against fungal infectious diseases. In this research antimycotic activity of the leaf extracts of five medicinal plants (Nerium indicum, Catheranthus roseus, Lantana camera, Ziziphus mauritiana) were tested against three dermatophytes (Trichophyton mentagrophytes, Trichophyton rubrum and Microsporum. gypseum). Development of more effective and less toxic antimycotic agents is required for the treatment of dermatophytosis. The plant materials were extracted with methanol, ethanol and diethyl ether solvent to investigate their antimycotic activities in Vitro. Ethanol and methanol extracts of all selected medicinal plants were showed the positive activity against all tested dermatophytes. Diethyl ether extract was showed lowest activity against T. mentagrophytes and T. rubrum and showed moderate activity against M. gypseum. The three dermatophytes differed with regard to their susceptibility to plant extracts.

Targeting Mediators Of Chronic Inflammation: A Promising Alternative Therapeutic Use Of Plant-Derived Remedies With Potent Bioactivity And Lower Cytotoxicity

2020 ◽  
Vol 154 (Supplement_1) ◽  
pp. S114-S115
Author(s):  
S Adebayo
Keyword(s):  
Medicinal Plants ◽  
Chronic Inflammation ◽  
Bioactive Molecules ◽  
Care Needs ◽  
Leaf Extracts ◽  
Promising Alternative ◽  
Cox 2 ◽  
Pharmacologically Active ◽  
Cox 1

Abstract Introduction/Objective Medicinal plants have a long history of use and has been exploited for basic health care needs for many centuries. The various parts of plants are rich sources of a plethora of pharmacologically active remedies that has remained largely unexplored for their therapeutic capabilities. Grewia mollis is one of the medicinal plants that are used to treat painful inflammatory conditions including rheumatism and associated fever. The inhibition of enzymes involved in prostaglandin (PG) synthesis are of great interest in combating chronic inflammation. Cyclooxygenases (COX) and Lipoxygenases (LOX), in particular COX-2 and 5-LOX utilize different pathways for PG synthesis and are important drivers of chronic inflammation. However, the use of conventional anti-inflammatory drugs and specific enzyme inhibitors against them are associated with severe adverse side-effects and organ toxicities. Therefore, attention has shifted to the search for potent and safer alternative strategies to treat chronic inflammation involving these mediators Methods The leaf part of G. mollis was extracted with solvents of varying polarities. The extracts were tested in vitro against COX-1, 2 and 5-LOX. In addition, the cytotoxicity and selectivity index of the plant extracts were evaluated using vero monkey kidney cells Results The hexane fraction of G. mollis leaf extracts had better inhibition against COX-1 (0,97±1,9 µg/mL) and COX-2 (1,13±0,2 µg/mL) when compared with the indomethacin control (1,30±0,6 µg/mL) and (1,52±0,2 µg/mL) respectively. Also, the butanol fraction inhibited 5-LOX (12,48±2,9 µg/mL) better than quercetin positive control (61,82±5,5 µg/mL). Overall, the extracts and fractions indicated reduced cytotoxicity (30,56-689,39 µg/mL) relative to the doxorubicin control (2,59 µg/mL). Conclusion The results indicated that extracts of G. mollis contained bioactive molecules capable of inhibiting the activities of C0X and 5-LOX with lower cytotoxicity. Pharmacologically active molecules with safety profile could be used, at least as a part of the template for remedies against mediators of chronic inflammation

Rapid determination of nonsteroidal anti-inflammatory properties of alpine medicinal plants (NAIP)

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
N Marcon ◽  
S Berthouzoz ◽  
F Zonnevijlle ◽  
AF Grogg
Keyword(s):  
Medicinal Plants ◽  
Rapid Determination ◽  
Anti Inflammatory

scholarly journals Leaf Extracts of Aerva lanata Inhibit the Activities of Type 2 Diabetes-Related Enzymes and Possess Antioxidant Properties

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Musbau Adewunmi Akanji ◽  
Samson Olasunkanmi Olukolu ◽  
Mutiu Idowu Kazeem
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Leaf Extracts ◽  
Chemically Induced ◽  
Radical Scavenging Ability ◽  
Aerva Lanata ◽  
Radical Scavenging Properties

The leaves of Aerva lanata are one of the indigenous medicinal plants used in the management of diabetes mellitus and its associated complications in Africa. However, its effect on the activities of diabetes-related enzymes has not been investigated. This study evaluated the in vitro inhibitory effects of different extracts of the A. lanata leaf on the activities of diabetes-related enzymes (α-amylase and α-glucosidase) and chemically induced free radicals. Aqueous, ethanol, and hydroethanol extracts of A. lanata leaves were subjected to a standard enzyme inhibition assay followed by determination of modes of inhibition of the enzymes. The antioxidant activities of the extracts were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). The results obtained showed that the hydroethanol extract of the A. lanata leaf optimally inhibited both α-amylase (IC50: 2.42 mg/mL) and α-glucosidase (IC50: 0.23 mg/mL). The Lineweaver-Burk plot revealed that the mode of inhibition of both enzymes by the hydroethanol extract was uncompetitive. However, the hydroethanol and aqueous extracts displayed the best DPPH and ABTS radical-scavenging ability, respectively. It can be concluded that the A. lanata extract inhibited the activities of both α-amylase and α-glucosidase uncompetitively, which may be attributed to its free radical-scavenging properties and rich phenolic composition.

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