Comparison of near infra-red spectroscopy, neutral detergent fibre assay and in-vitro organic matter digestibility assay for rapid determination of the biochemical methane potential of meadow grasses

2011 ◽  
Vol 102 (17) ◽  
pp. 7835-7839 ◽  
Author(s):  
Chitra Sangaraju Raju ◽  
Alastair James Ward ◽  
Lisbeth Nielsen ◽  
Henrik Bjarne Møller
Keyword(s):  
Organic Matter ◽  
Rapid Determination ◽  
Biochemical Methane Potential ◽  
Neutral Detergent Fibre ◽  
Infra Red ◽  
Methane Potential ◽  
Organic Matter Digestibility

A MODIFIED METHOD FOR THE IN VITRO DETERMINATION OF DRY MATTER AND ORGANIC MATTER DIGESTIBILITY

1973 ◽  
Vol 53 (2) ◽  
pp. 251-256 ◽  
Author(s):  
R. E. LARSEN ◽  
G. M. JONES
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
In Vitro Fermentation ◽  
Modified Method ◽  
Initial Stage ◽  
Fermentation Period ◽  
Organic Matter Digestibility ◽  
Stage 1

Several modifications were made to the two-stage in vitro fermentation techniques for dry matter and organic matter digestibility (IVDMD and IVOMD) determinations developed by Tilley and Terry (J. Brit. Grassland Soc. 18: 104–111, 1963) and Alexander and McGowan (J. Brit. Grassland Soc. 21: 140–147, 1966). These modifications included: (1) changing the buffer medium, which resulted in a pH of 6.8–7.0 in the fermentation tubes during the initial (stage 1) 48-h fermentation period, and (2) shortening the acid-pepsin incubation period from 48 to 24 h, and thus reducing time for estimation of both IVDMD and IVOMD by 24 h. Modification (1) eliminated pH adjustments during fermentation and acidification at the end of the fermentation period. Acid-pepsin digestion of substrates was completed within 24 h instead of 48 h. Both IVDMD and IVOMD values were obtained on the same substrates. These modifications were evaluated using 65 samples that arose from 13 forages, comprised of corn and grass silages and their mixtures, which had been dried by five different methods. IVDMD and IVOMD were determined on all samples, comparing the modified method with the parent methods. IVDMD and IVOMD coefficients within each method were not statistically different between the Tilley and Terry method and the modified method.

Comparing estimates of herbage digestibility from faecal nitrogen and in vitro determinations

1980 ◽  
Vol 31 (1) ◽  
pp. 57-62
Author(s):  
H. A. Birrell
Keyword(s):  
Organic Matter ◽  
Digestible Organic Matter ◽  
Faecal Nitrogen ◽  
Feeding Trials ◽  
Grazing Sheep ◽  
Organic Matter Digestibility ◽  
Image Position ◽  
Stocking Rates

ABSTRACTIn a grazing experiment, the organic matter digestibility of herbage selected by sheep grazing at two stocking rates was determined at monthly intervals over a 9-month period by (i) the in vitro determination of organic matter digestibility of the material collected from sheep fitted with an oesophageal fistula, and (ii) derivation from the prediction of digestible organic matter intake with a faecal nitrogen regression. A comparison of the two techniques confirms the view of other workers that faecal nitrogen indices based on pen feeding trials of green herbage are unreliable for predicting digestibility of green herbage in the field. The bias which is produced by faecal nitrogen regression appears to be associated with the time spent grazing by the animal each day. Relating organic matter digestibility directly to nitrogen content of faeces of grazing sheep (N, g nitrogen/100 g faecal organic matter) and their grazing time (T,h) yielded the following equation:The expression has standard errors (±2·6) similar to those involved in an in vitro determination. It provides a simple technique for estimating digestibility of herbage eaten by grazing sheep, but this may only be applicable to the particular sward conditions.Field estimates of digestibility over summer and autumn by this equation were found to be similar to estimates by a faecal nitrogen expression established from hand feeding sheep with dry summer herbage.

Prediction of the organic matter digestibility of forages in horses by the gas test method

1998 ◽  
Vol 22 ◽  
pp. 252-254 ◽  
Author(s):  
D. Macheboeuf ◽  
M. Jestin ◽  
J. Andrieu ◽  
W. Martin-Rosset
Keyword(s):  
Organic Matter ◽  
Chemical Composition ◽  
Rumen Fluid ◽  
Gas Production ◽  
Test Method ◽  
Energy Value ◽  
Infra Red ◽  
Organic Matter Digestibility ◽  
Better Than

In horses, forages account for the greatest proportion of the diet (Micol and Martin-Rosset, 1995). As a result, the energy value of forages has to be determined very accurately to meet the horse requirements. In the UFC system (Horse Feed Unit) proposed by INRA (Martin-Rosset et al., 1994), the energy value of forages is closely related to organic matter digestibility (OMD).In ruminants, OMD has been related to the in vitro gas production after 24 h of fermentation of foodstuffs with rumen fluid (Menke et al., 1979). The prediction of OMD with the gas test method is less accurate than with the nylon bag method but better than prediction performed with in vitro method or from chemical composition of foods (Khazaal et al., 1993 and 1995).In the UFC system, three routine methods have been performed by our laboratory and proposed by INRA to predict routinely OMD of foodstuffs. OMD can be predicted either from chemical composition (Martin-Rosset et al., 1996a), by a pepsin cellulase method (Aufrère, 1982; Martin-Rosset et al., 1996b) or by near infra-red spectrometry (NIRS) (Andrieu et al., 1996).The gas test method was subjected to be adapted in horses for predicting OMD. The accuracy of the prediction with the gas test method was compared with the other routine methods.

Performance of lambs and the incidence of staggers and heat stress on two perennial ryegrass (Lolium perenne) cultivars using a leader-follower rotational grazing management system

2001 ◽  
Vol 136 (1) ◽  
pp. 99-110 ◽  
Author(s):  
S. J. BLUETT ◽  
J. HODGSON ◽  
P. D. KEMP ◽  
T. N. BARRY
Keyword(s):  
Organic Matter ◽  
Heat Stress ◽  
Lolium Perenne ◽  
Perennial Ryegrass ◽  
Nutritive Value ◽  
Fibre Content ◽  
Serum Prolactin ◽  
Neutral Detergent Fibre ◽  
Organic Matter Digestibility

A summer lamb finishing experiment at Palmerston North, New Zealand compared the performance and health of weaned lambs grazing Aries HD or Yatsyn 1 perennial ryegrass (Lolium perenne). Aries HD ryegrass was originally selected for increased digestibility over summer and early autumn. Two groups of Romney×Suffolk lambs (15 per group) were rotationally grazed on each cultivar in a leader-follower sequence to assess both the nutritive value of the pastures and the alkaloid challenge imposed by the respective cultivar/endophyte (Neotyphodium lolii) associations. Herbage from leader pastures contained a higher content of ryegrass (600 v. 474±27·3 g/kg, P < 0·001) and a lower content of dead material (355 v. 479±34·2 g/kg, P < 0·001) than follower pastures. The in vitro organic matter digestibility coefficient of leader pastures was greater (0·62 v. 0·55±0·024, P < 0·05) and the neutral detergent fibre content lower (592 v. 631±11·4 g/kg, P < 0·005) than follower pastures. Consequently, the leader lambs had faster liveweight gains (92 v. 53±10·6 g/day, P < 0·0005) than the follower lambs. Aries HD and Yatsyn 1 cultivars were similar in sward composition, in vitro organic matter digestibility, neutral detergent fibre content and nitrogen content. Lambs grazing Aries HD had a higher herbage intake in late January than lambs grazing Yatsyn 1 pasture (0·95 v. 0·85±0·023 kg OM/day, P < 0·005). Overall, the liveweight gains of lambs grazing Aries HD and Yatsyn 1 were similar (71 v. 73±10·6 g/day, P > 0·7).Grazing sequence and cultivar did not affect the concentrations of lolitrem B and peramine from herbage cut to ground level. However, serum prolactin concentration, which can indicate ergovaline intoxication, was lower in lambs grazing follower swards (181 v. 120±18·0 ng/ml, P < 0·005). Ergovaline concentration in Aries HD herbage was about half the concentration detected in Yatsyn 1 herbage (0·25 v. 0·49±0·050 mg/kg, P < 0·001). In addition, respiration rate, which can be an indicator of heat stress, was higher in lambs grazing Yatsyn 1 than Aries HD (77 v. 74±1·1 breaths per minute, P < 0·005). All lambs on all treatments had clinical ryegrass staggers from 10 February to 9 March. Lambs were removed from the experiment on 9 March. Both grazing sequence and cultivar affected the severity of ryegrass staggers. Staggers score was highest in Yatsyn 1 followers (4·4) and lowest in Aries HD leaders (3·1). Both grazing sequence and cultivar affected the alkaloid challenge imposed on lambs, resulting in differences in staggers severity, heat stress and performance of the lambs over the summer and autumn period.

Determination of methane yield of cellulose using different experimental setups

2014 ◽  
Vol 70 (4) ◽  
pp. 599-604 ◽  
Author(s):  
Bing Wang ◽  
Ivo Achu Nges ◽  
Mihaela Nistor ◽  
Jing Liu
Keyword(s):  
Water Column ◽  
Test System ◽  
Methane Yield ◽  
Biochemical Methane Potential ◽  
Methane Potential ◽  
Bmp Test ◽  
Potential Test

In this work, biochemical methane potential (BMP) tests with cellulose as a model substrate were performed with the aid of three manually operated or conventional experimental setups (based on manometer, water column and gas bag) and one automated apparatus specially designed for analysis of BMP. The methane yields were 340 ± 18, 354 ± 13, 345 ± 15 and 366 ± 5 ml CH4/g VS obtained from experimental setups with manometer, water column, gas bag, and automatic methane potential test system, which corresponded to a biodegradability of 82, 85, 83 and 88% respectively. The results demonstrated that the methane yields of cellulose obtained from conventional and automatic experimental setups were comparable; however, the methane yield obtained from the automated apparatus showed greater precision. Moreover, conventional setups for the BMP test were more time- and labour-intensive compared with the automated apparatus.

The prediction of digestibility by rumen fluid and enzymatic methods

1996 ◽  
Vol 1996 ◽  
pp. 211-211
Author(s):  
Peter Young ◽  
F. P. O'Mara ◽  
M. Rath ◽  
P. J. Caffrey
Keyword(s):  
Organic Matter ◽  
Rumen Fluid ◽  
Organic Matter Digestibility ◽  
Enzymatic Methods

Rumen fluid and cellulase based techniques are widely used to predict the digestibility of compound feeds and their ingredients. Recently gammanase enzymes have been added to some cellulase based techniques (Dowman, 1993; De Boever et al., 1994). Few comparisons of these techniques have involved by-product concentrate ingredients. The objective of this experiment was to compare the ability of three techniques, in vitro rumen fluid (RF), pepsin cellulase gammanase (PCG), and neutral detergent cellulase gammanase (NCDG), to predict the in vivo organic matter digestibility (OMD) of concentrate ingredients.

scholarly journals Preparation of wastelage with poultry droppings and rice straw (Oryza sativa L.) as a cattle feed

2018 ◽  
Vol 4 (3) ◽  
pp. 251-258
Author(s):  
Obydul Islam ◽  
Somaya Akter ◽  
Md Ahidul Islam ◽  
Dewan Kamruzzaman Jamee ◽  
Rokibul Islam Khan
Keyword(s):  
Organic Matter ◽  
Oryza Sativa ◽  
Rice Straw ◽  
Oryza Sativa L ◽  
Fungal Growth ◽  
Metabolizable Energy ◽  
Organic Matter Digestibility ◽  
Ph Level ◽  
Poultry Droppings

The use of poultry droppings as a feed ingredient may not only reduce waste and environmental pollution but also provide inexpensive feed components for ruminants. An experiment was conducted to prepare wastelage in the field laboratory of Animal Science Department, Bangladesh Agricultural University, Mymensingh. Rice straw (Oryza sativa L.) was mixed with 0%, 10%, 20% and 30% caged layer excreta (CLE) and 5% molasses in each treatment on dry matter (DM) basis and ensiled in air tight container under room temperature. After 60 days, ensiled mixture was opened. All the wastelage had desirable smell, light brownish color, soft texture and no fungal growth was found. Results revealed that PH, DM, crude protein (CP), crude fiber (CF), ash, in vitro organic matter digestibility and metabolizable energy were significantly (p<0.05) influenced by different levels of CLE. The highest CP (5.97g/100g DM) was observed in wastelage with 30% CLE (p<0.05) followed by 20% and 10% CLE. The PH level, DM, Ash and CF contents were decreased linearly (p<0.05) from 4.8 to 4.11, 78.00 to 55.63, 25.08 to 17.70 and 22.57 to 14.95%, respectively as the level of CLE increased from 0 to 30%. In all treatments, EE content was not significantly influenced by the different level of CLE. The in vitro organic matter digestibility (IVOMD) and metabolizable energy (ME) also increased significantly (p<0.05) with the increased level of CLE and maximum value (57.51%, and 8.12 MJ/Kg DM, respectively) was obtained in wastelage with 10% CLE, which is statistically identical with 20% CLE. Therefore, it could be speculated that ensiling rice straw with up to 20% CLE improved the feeding value of wastelage.Asian J. Med. Biol. Res. September 2018, 4(3): 251-258

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