scholarly journals Morphology Performance On Six Black Rice Accessions (Oryza Sativa L.) In M1 Generation Irradiated By Gamma Rays

2021 ◽  
Vol 3 (2) ◽  
pp. 62-67
Author(s):  
Roza Yunita ◽  
Siti Nurhidayah
Keyword(s):  
Genetic Diversity ◽  
Oryza Sativa ◽  
Gamma Rays ◽  
Gamma Ray ◽  
Oryza Sativa L ◽  
Seedling Height ◽  
Black Rice ◽  
Germination Characteristics ◽  
Gamma Ray Irradiation ◽  
Materials Used

Increasing the genetic diversity of plants can be done through physical mutations with gamma-ray irradiation. This study aims to determine gamma-ray irradiation on the growth of six M1 generation black rice accessions. This research was conducted in May-October 2019 at the Experimental Rice Fields of the Tasikmalaya Perjuangan University, West Java. The materials used in this study were six accessions from Tasikmalaya (PH, PH2, PH3, PH5, PH7, and PH8) without 0 Gy irradiation and with 200 Gy gamma-ray irradiation of M1 generation. The results showed that gamma-ray irradiation reduced germination characteristics, seedling height, and flowering age. Some of the accessions showed different effects; there were a decrease and an increase in the characters of root length, plant height, number of productive tillers, number of unproductive tillers, and the total number of tillers.

scholarly journals The growth and leaf chlorophyll in black rice (Oryza sativa L. Indica) plants induced by gamma rays as responses to the drought stress condition

2021 ◽  
Vol 905 (1) ◽  
pp. 012016
Author(s):  
E Purwanto ◽  
A M Ula ◽  
Parjanto
Keyword(s):  
Oryza Sativa ◽  
Drought Stress ◽  
Chlorophyll Content ◽  
Gamma Rays ◽  
Gamma Ray ◽  
Oryza Sativa L ◽  
Black Rice ◽  
Leaf Chlorophyll ◽  
Drought Stress Condition ◽  
Gamma Ray Irradiation

Abstract The aims of this study to determine the growth and leaf chlorophyll content in black rice (Oryza sativa L. Indica) which were induced by gamma rays as responses to the drought stress conditions. This study was carried out on July–November 2020, at the experimental field (screenhouse), Medicinae Street, UNS Perum, Jati Village, Jaten District, Karanganyar Regency. Meanwhile, the chlorophyll content test was carried out at Plant Tissue Culture and Biotechnology Laboratory, Faculty of Agriculture, Universitas Sebelas Maret. The study was a field trial research design without replication. The research treatments were 24 genotypes as follows: 10 genotypes of 100 gray gamma-ray irradiation with 2 control genotypes; and 10 genotypes of 200 gray gamma-irradiation with 2 control genotypes. The results showed that the plant height was lower in plants with irradiation compared to the Control-01 plant. The crown-root ratio showed irradiated plants had higher average results than control plants. The leaf chlorophyll of 100 gray irradiated plants showed higher results than control; while 200 gray irradiated plants showed similar results.

scholarly journals Kualitas Fisik Beras (Oryza sativa L.) Mutan M4 Hasil Iradiasi Sinar Gamma

2020 ◽  
Vol 4 (1) ◽  
pp. 32-42
Author(s):  
Nanda Funna Ledita ◽  
Efendi Efendi ◽  
Rita Hayati
Keyword(s):  
Oryza Sativa ◽  
Gamma Ray ◽  
Amylose Content ◽  
Oryza Sativa L ◽  
Mutant Line ◽  
Milled Rice ◽  
Physical Quality ◽  
Significant Difference ◽  
Gamma Ray Irradiation

Abstrak.  Beras (Oryza sativa L.) merupakan salah satu tanaman pangan yang berfungsi sebagai tanaman pokok selain gandum dan jagung di Indonesia, dikarenakan mengandung nutrisi yang dibutuhkan tubuh seperti karbohidrat, protein, air  dan lemak. Meningkatnya jumlah penduduk maka permintaan terhadap beras semakin meningkat. Permintaan tersebut sangat ditentukan oleh selera konsumen yang dipengaruhi oleh kualitas beras yaitu mutu fisik (ukuran dan bentuk butiran, rendemen dan pengapuran), mutu tanak (kadar amilosa dan suhu gelatinisasi) dan mutu rasa (tekstur dan aroma). Penelitian ini bertujuan untuk mengetahui kualitas beberapa galur padi mutan M4 hasil iradiasi sinar gamma. Penelitian ini dilaksanakan di Laboratorium Ilmu dan Teknologi Benih, Laboratorium Analisis Pangan dan Hasil Pertanian, Laboratorium Ilmu Nutrisi dan Teknologi Pakan dan Laboratorium Fisiologi Tumbuhan Fakultas Pertanian Universitas Syiah Kuala, serta Laboratorium Departemen Ilmu dan Teknologi Pangan Fakultas Teknologi Pertanian Institut Pertanian Bogor, yang berlangsung pada bulan April hingga Juli 2018. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) pola non faktorial dengan 3 ulangan. Perlakuan penelitian ini berupa galur (G) dengan 5 taraf, yaitu i-Snb (G0) kontrol, O-39e (G1), O-57d (G2), O-57e (G3) dan O-88j (G4). Parameter yang diamati yaitu sifat fisik beras (densitas kamba, derajat sosoh, dimensi beras rendemen beras giling, dan warna). Hasil penelitian menunjukkan bahwa galur  mutan M4 berpengaruh sangat nyata terhadap kualitas sifat fisik rendemen beras giling dan warna nilai L (kecerahan). Rendemen beras giling terbaik galur O-39e (70,33%) dan warna nilai L (kecerahan) galur O-88j (117,90).  Perlakuan terbaik secara kumulatif  untuk sifat fisik beras yaitu pada i-Snb.. Physical Quality of Rice (Oryza sativa L. ) M4  Mutant Results of Gamma Ray IrradiationAbstract. Rice (Oryza sativa L.) is one of main food which the function is to be staple food beside wheat and corn in Indonesia because it contains nutrients such as carbohydrates, protein, water, and fat. Towards the increasing populations, the demand of rice is increasing as well. The demand is largerly depends on the consumer taste affected by the quality of rice which are physical quality (size and grain shape, rendemen, and calcification), cooked quality (amylose content and gelatinization temperature) and flavor quality (texture and smell). The purpose of this research was to investigate the quality of rice from several M4 mutant line resulting from gamma ray irradiation. This research was conducted at the Laboratory of Seed Science and Technology and Laboratory of Food and Agricultural Analysis, Faculty of Agriculture, Syiah Kuala University, Banda Aceh, from the month April to July 2018. The design used in this study was based on a non-factorial completely randomize design with 3 replications and continued with the Least Significant Difference Test (LSD) level of 5% on a significant F test results. The results showed that the M4 mutant line had a very significant effect on the quality of physical properties, the yield of milled rice and the best color L (brightness), namely on the O-88j and O-39e line.

scholarly journals Genetic Variation Analysis of Early-heading Plant (Oryza sativa L.) Lines Derived from Gamma-ray Irradiation

2012 ◽  
Vol 25 (1) ◽  
pp. 142-151 ◽  
Author(s):  
Jai-Hyunk Ryu ◽  
Hyun-Su So ◽  
Jae-Il Lyu ◽  
Oh-Do Kwon ◽  
Young-Il Lee ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Oryza Sativa ◽  
Gamma Ray ◽  
Oryza Sativa L ◽  
Variation Analysis ◽  
Gamma Ray Irradiation

scholarly journals Subcellular Localization and Vesicular Structures of Anthocyanin Pigmentation by Fluorescence Imaging of Black Rice (Oryza sativa L.) Stigma Protoplast

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 685
Author(s):  
Enerand Mackon ◽  
Yafei Ma ◽  
Guibeline Charlie Jeazet Dongho Epse Mackon ◽  
Qiufeng Li ◽  
Qiong Zhou ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Subcellular Localization ◽  
Oryza Sativa L ◽  
Scientific Evidence ◽  
Brown Rice ◽  
The Body ◽  
Central Vacuole ◽  
In Planta ◽  
Black Rice

Anthocyanins belong to the group of flavonoid compounds broadly distributed in plant species responsible for attractive colors. In black rice (Oryza sativa L.), they are present in the stems, leaves, stigmas, and caryopsis. However, there is still no scientific evidence supporting the existence of compartmentalization and trafficking of anthocyanin inside the cells. In the current study, we took advantage of autofluorescence with anthocyanin’s unique excitation/emission properties to elucidate the subcellular localization of anthocyanin and report on the in planta characterization of anthocyanin prevacuolar vesicles (APV) and anthocyanic vacuolar inclusion (AVI) structure. Protoplasts were isolated from the stigma of black and brown rice and imaging using a confocal microscope. Our result showed the fluorescence displaying magenta color in purple stigma and no fluorescence in white stigma when excitation was provided by a helium–neon 552 nm and emission long pass 610–670 nm laser. The fluorescence was distributed throughout the cell, mainly in the central vacuole. Fluorescent images revealed two pools of anthocyanin inside the cells. The diffuse pools were largely found inside the vacuole lumen, while the body structures could be observed mostly inside the cytoplasm (APV) and slightly inside the vacuole (AVI) with different shapes, sizes, and color intensity. Based on their sizes, AVI could be grouped into small (Ф < 0.5 um), middle (Ф between 0.5 and 1 um), and large size (Ф > 1 um). Together, these results provided evidence about the sequestration and trafficking of anthocyanin from the cytoplasm to the central vacuole and the existence of different transport mechanisms of anthocyanin. Our results suggest that stigma cells are an excellent system for in vivo studying of anthocyanin in rice and provide a good foundation for understanding anthocyanin metabolism in plants, sequestration, and trafficking in black rice.

Irradiation-Induced Genetic Diversity of Leaf Color Mutation in Rice (Oryza sativa L)

2000 ◽  
Vol 28 (3) ◽  
pp. 255-262
Author(s):  
Dianxing Wu ◽  
Qingyao Shu ◽  
Yingwu Xia
Keyword(s):  
Genetic Diversity ◽  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Leaf Color ◽  
Color Mutation
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