190 Single nucleotide polymorphisms in the signal transducer and regulator of transcription (STAT) genes are associated with milk production, milk composition, and fertility traits in Holstein Friesian cattle

2017 ◽  
Vol 95 (suppl_4) ◽  
pp. 94-94 ◽  
Author(s):  
L. Ratcliffe ◽  
M. Mullen ◽  
M. C. McClure ◽  
J. McClure ◽  
F. Kearney
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Milk Production ◽  
Milk Composition ◽  
Signal Transducer ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Holstein Friesian ◽  
Fertility Traits ◽  
Friesian Cattle

scholarly journals Single nucleotide polymorphisms at the imprinted bovine insulin-like growth factor 2 (IGF2) locus are associated with dairy performance in Irish Holstein-Friesian cattle

2010 ◽  
Vol 78 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Erik W Berkowicz ◽  
David A Magee ◽  
Klaudia M Sikora ◽  
Donagh P Berry ◽  
Dawn J Howard ◽  
...  
Keyword(s):  
Body Size ◽  
Growth Factor ◽  
Single Nucleotide Polymorphisms ◽  
Dairy Cattle ◽  
Milk Production ◽  
Milk Fat ◽  
Milk Protein ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Holstein Friesian

The imprinted insulin-like growth factor 2 gene (IGF2) encodes a fetal mitogenic hormone protein (IGF-II) and has previously been shown to be associated with performance in dairy cattle. In this study we assessed genotype-phenotype associations between four single nucleotide polymorphisms (SNPs) located within the bovine IGF2 locus on chromosome 29 and a range of performance traits related to milk production, animal growth and body size, fertility and progeny survival in 848 progeny-tested Irish Holstein-Friesian sires. Two of the four SNPs (rs42196909 and IGF2.g-3815A>G), which were in strong linkage disequilibrium (r2=0·995), were associated with milk yield (P⩽0·01) and milk protein yield (P⩽0·05); the rs42196901 SNP was also associated (P⩽0·05) with milk fat yield. Associations (P⩽0·05) with milk fat percentage and milk protein percentage were observed at the rs42196901 and IGF2.g-3815A>G SNPs, respectively. The rs42196909 and IGF2.g-3815A>G SNPs were also associated with progeny carcass conformation (P⩽0·05), while an association (P⩽0·01) with progeny carcass weight was observed at the rs42194733 SNP locus. None of the four SNPs were associated with body size, fertility and progeny survival. These findings support previous work which suggests that the IGF2 locus is an important biological regulator of milk production in dairy cattle and add to an accumulating body of research showing that imprinted genes influence many complex performance traits in cattle.

scholarly journals Impact of SNPs in ACACA, SCD1, and DGAT1 Genes on Fatty Acid Profile in Bovine Milk with Regard to Lactation Phases

Animals ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 997
Author(s):  
Marzena M. Kęsek-Woźniak ◽  
Edyta Wojtas ◽  
Anna E. Zielak-Steciwko
Keyword(s):  
Milk Fat ◽  
Bovine Milk ◽  
Milk Composition ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Holstein Friesian ◽  
Dgat1 Gene ◽  
Health Promoting ◽  
Lactation Phase ◽  
Friesian Cattle

Milk fat is a dietary source of fatty acids (FA), which can be health promoting or can increase risks of some diseases. FA profile composition depends on many factors, among them gene polymorphism. This study analyzed the relation between polymorphism of acetyl-CoA carboxylase α (ACACA), stearoyl-CoA desaturase 1 (SCD1), diacylglycerol acyltransferase 1 (DGAT1) genes with FA profile in milk from Polish Holstein-Friesian cattle and determined changes of FA percentage during lactation with regard to polymorphism. Milk samples were collected twice: during the first phase of lactation (<90 Days in milk; DIM) and at the end of lactation (>210 DIM). During the first milk collection, blood samples were taken to analyze three chosen single nucleotide polymorphisms (SNPs): AJ312201.1g.1488C > G SNP in ACACA gene, A293V SNP in SCD1 gene, and K232A SNP in DGAT1 gene. Increased concentration of FA that are less beneficial for human health and have lower concentration of healthy FA in homozygotes: GG in ACACA, VV in SCD1, and KK in DGAT1 were observed, as well as a strong influence of the analyzed genes on FA with 18C atoms was also found. Moreover, it was demonstrated that lactation phase significantly affected FA percentage in milk depending on the phenotype. These results may contribute their part to knowledge toward obtaining more beneficial milk composition.

scholarly journals Genetic Association Analysis of Paratuberculosis Forms in Holstein-Friesian Cattle

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Patricia Vázquez ◽  
Otsanda Ruiz-Larrañaga ◽  
Joseba M. Garrido ◽  
Mikel Iriondo ◽  
Carmen Manzano ◽  
...  
Keyword(s):  
Multiple Testing ◽  
Additive Model ◽  
Nuclear Body ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Body Protein ◽  
Holstein Friesian ◽  
Solute Carrier ◽  
Friesian Cattle ◽  
Oligomerization Domain

A genetic susceptibility toMycobacterium aviumsubsp.paratuberculosis(MAP) infections in ruminants has been longtime suspected to exist. Recently, natural infections in cattle have been reclassified intolatentandpatentforms based on histopathological findings and their associations with immunological and microbiological variables. This study aims to explore whether these newly defined phenotypes are associated with twenty-four single-nucleotide polymorphisms (SNPs) in six bovine candidate genes:nucleotide-binding oligomerization domain 2 (NOD2), solute carrier family 11 member A1 (SLC11A1), nuclear body protein SP110 (SP110), toll-like receptors (TLRs) 2and4, andCD209(also known asDC-SIGN, dendritic cell-specific ICAM3-grabbing nonintegrin). SNPs were genotyped for 772 Holstein-Friesian animals (52.6%apparently free; 38.1%latent; 9.3%patent) by TaqMan OpenArray technology. Genotypic-phenotypic associations were assessed by logistic regression analysis adjusted for age at slaughter, under five models (codominant, dominant, recessive, overdominant, and log-additive), and corrected for multiple testing. The rs208222804 C allele (CD209gene) was found to be associated withlatentparatuberculosis (log-additive model:P<0.0034after permutation procedure; OR = 0.64, 95% CI = 0.48–0.86). No significant association was detected between any SNP and thepatentphenotype. Consequently,CD209gene may play a key role in the pathogenesis of bovine paratuberculosis.

Identification of 14 new single nucleotide polymorphisms in the bovineSLC27A1gene and evaluation of their association with milk fat content

2008 ◽  
Vol 75 (2) ◽  
pp. 129-134 ◽  
Author(s):  
Laura Ordovas ◽  
Pilar Zaragoza ◽  
Juan Altarriba ◽  
Clementina Rodellar
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Milk Fat ◽  
Energy Homeostasis ◽  
Integral Membrane Protein ◽  
Fat Content ◽  
Sequencing Analysis ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Isolation And Characterization ◽  
Holstein Friesian

The solute carrier family 27 member 1 (SLC27A1) is an integral membrane protein involved in the transport of long-chain fatty acids across the plasma membrane. This protein has been implicated in diet-induced obesity and is thought to be important in the control of energy homeostasis. In previous reports, our group described the isolation and characterization of the bovineSLC27A1gene. The bovine gene is organized in 13 exons spanning over more than 40 kb of genomic DNA and maps in BTA 7 where several quantitative trait loci for fat related traits have been described. Because of its key role in lipid metabolism and its genomic localization, in the present work the search for variability in the bovineSLC27A1gene was carried out with the aim of evaluating its potential association with milk fat content in dairy cattle. By sequencing analysis of all exons and flanking regions 14 new single nucleotide polymorphisms (SNPs) were identified: 1 in the promoter, 7 in introns and 6 in exons. Allele frequencies of all the SNPs were calculated by minisequencing analysis in two groups of Holstein-Friesian animals with highest and lowest milk-fat content estimated breeding values as well as in animals of two Spanish cattle breeds, Asturiana de los Valles and Menorquina. In the conditions assayed, no significant differences between Holstein-Friesian groups were found for any of the SNPs, suggesting that theSLC27A1gene may have a poor or null effect on milk fat content. In Asturiana and Menorquina breeds all the positions were polymorphic with the exception of SNPs 1 and 8 in which C allele was fixed in both of them.

scholarly journals Association of genetic variants in STAT5B gene with milk performance and mastitis related traits in dairy cattle

2021 ◽  
Vol 91 (5) ◽  
pp. 459-471
Author(s):  
Nawab Ali ◽  
◽  
Sadaf Niaz ◽  
Irfan Khattak ◽  
Naimat U. Khan ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Association Analysis ◽  
Milk Composition ◽  
Clinical Mastitis ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Exon 2 ◽  
Holstein Friesian ◽  
Hardy Weinberg Equilibrium ◽  
Milk Performance

Udder performance and health are important traits in dairy cattle worldwide. The present study aimed to explore the association of single nucleotide polymorphisms (SNPs) in the STAT5B gene in dairy cattle with milk performance and mastitis related traits. The study included 201 cows of three pure breeds (i.e. Holstein Friesian, Jersey and Achai) and two crossbred cattle at four established dairy farms in Khyber Pakhtunkhwa, Pakistan. The milk samples were analysed for somatic cell count (SCC) and milk composition (i.e. fat , protein and lactose percentages). The generalized linear model was deployed for association analysis using SAS. The pool DNA sequencing showed four (three synonymous and a 3/ UTR) SNPs in STAT5B. These SNPs were further validated in all DNA samples using SNaPshot assay. The breed-wise analysis showed that most of the SNPs were consistent with Hardy-Weinberg equilibrium (P>0.05). The association analysis revealed a significantly higher protein percentage in TT genotype and lower SCC in CC genotypes of SNP 1 (exon 2, C>T), whereas in SNP 2 (exon 16, T>C) the TT genotypes revealed significantly lower SCC and SCS compared with other genotypes (P<0.05). SNP 4 (3/ UTR, C>T) showed significantly lower SCC and frequency of clinical mastitis in the heterozygous (CT) genotype compared to the homozygous genotypes. The in silico predictions revealed changes in the RNA secondary structure for SNP 2 and SNP 3. The study suggests that STAT5B should be considered as a candidate gene, and the variants identified as useful genetic markers for improved milk composition and udder health.

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