Single nucleotide polymorphisms in the CXCR1 gene and its association with clinical mastitis incidence in Polish Holstein-Friesian cows

The solute carrier family 27 member 1 (SLC27A1) is an integral membrane protein involved in the transport of long-chain fatty acids across the plasma membrane. This protein has been implicated in diet-induced obesity and is thought to be important in the control of energy homeostasis. In previous reports, our group described the isolation and characterization of the bovineSLC27A1gene. The bovine gene is organized in 13 exons spanning over more than 40 kb of genomic DNA and maps in BTA 7 where several quantitative trait loci for fat related traits have been described. Because of its key role in lipid metabolism and its genomic localization, in the present work the search for variability in the bovineSLC27A1gene was carried out with the aim of evaluating its potential association with milk fat content in dairy cattle. By sequencing analysis of all exons and flanking regions 14 new single nucleotide polymorphisms (SNPs) were identified: 1 in the promoter, 7 in introns and 6 in exons. Allele frequencies of all the SNPs were calculated by minisequencing analysis in two groups of Holstein-Friesian animals with highest and lowest milk-fat content estimated breeding values as well as in animals of two Spanish cattle breeds, Asturiana de los Valles and Menorquina. In the conditions assayed, no significant differences between Holstein-Friesian groups were found for any of the SNPs, suggesting that theSLC27A1gene may have a poor or null effect on milk fat content. In Asturiana and Menorquina breeds all the positions were polymorphic with the exception of SNPs 1 and 8 in which C allele was fixed in both of them.

Download Full-text

Gene Expression Profile of Oocytes Using Microarray and Single Nucleotide Polymorphisms of Stat3 and Odc1 Genes in Sperms Linked With Their Quality in Holstein-Friesian

Annals of Agricultural Science, Moshtohor ◽

10.21608/assjm.2021.183714 ◽

2021 ◽

Vol 59 (2) ◽

pp. 101-102

Author(s):

Nasser Ghanem

Keyword(s):

Gene Expression ◽

Single Nucleotide Polymorphisms ◽

Gene Expression Profile ◽

Expression Profile ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Holstein Friesian

Download Full-text

Association of genetic variants in STAT5B gene with milk performance and mastitis related traits in dairy cattle

Veterinarski arhiv ◽

10.24099/vet.arhiv.1038 ◽

2021 ◽

Vol 91 (5) ◽

pp. 459-471

Author(s):

Nawab Ali ◽

◽

Sadaf Niaz ◽

Irfan Khattak ◽

Naimat U. Khan ◽

...

Keyword(s):

Dairy Cattle ◽

Association Analysis ◽

Milk Composition ◽

Clinical Mastitis ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Exon 2 ◽

Holstein Friesian ◽

Hardy Weinberg Equilibrium ◽

Milk Performance

Udder performance and health are important traits in dairy cattle worldwide. The present study aimed to explore the association of single nucleotide polymorphisms (SNPs) in the STAT5B gene in dairy cattle with milk performance and mastitis related traits. The study included 201 cows of three pure breeds (i.e. Holstein Friesian, Jersey and Achai) and two crossbred cattle at four established dairy farms in Khyber Pakhtunkhwa, Pakistan. The milk samples were analysed for somatic cell count (SCC) and milk composition (i.e. fat , protein and lactose percentages). The generalized linear model was deployed for association analysis using SAS. The pool DNA sequencing showed four (three synonymous and a 3/ UTR) SNPs in STAT5B. These SNPs were further validated in all DNA samples using SNaPshot assay. The breed-wise analysis showed that most of the SNPs were consistent with Hardy-Weinberg equilibrium (P>0.05). The association analysis revealed a significantly higher protein percentage in TT genotype and lower SCC in CC genotypes of SNP 1 (exon 2, C>T), whereas in SNP 2 (exon 16, T>C) the TT genotypes revealed significantly lower SCC and SCS compared with other genotypes (P<0.05). SNP 4 (3/ UTR, C>T) showed significantly lower SCC and frequency of clinical mastitis in the heterozygous (CT) genotype compared to the homozygous genotypes. The in silico predictions revealed changes in the RNA secondary structure for SNP 2 and SNP 3. The study suggests that STAT5B should be considered as a candidate gene, and the variants identified as useful genetic markers for improved milk composition and udder health.

Download Full-text

Single nucleotide polymorphisms at the imprinted bovine insulin-like growth factor 2 (IGF2) locus are associated with dairy performance in Irish Holstein-Friesian cattle

Journal of Dairy Research ◽

10.1017/s0022029910000567 ◽

2010 ◽

Vol 78 (1) ◽

pp. 1-8 ◽

Cited By ~ 16

Author(s):

Erik W Berkowicz ◽

David A Magee ◽

Klaudia M Sikora ◽

Donagh P Berry ◽

Dawn J Howard ◽

...

Keyword(s):

Body Size ◽

Growth Factor ◽

Single Nucleotide Polymorphisms ◽

Dairy Cattle ◽

Milk Production ◽

Milk Fat ◽

Milk Protein ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Holstein Friesian

The imprinted insulin-like growth factor 2 gene (IGF2) encodes a fetal mitogenic hormone protein (IGF-II) and has previously been shown to be associated with performance in dairy cattle. In this study we assessed genotype-phenotype associations between four single nucleotide polymorphisms (SNPs) located within the bovine IGF2 locus on chromosome 29 and a range of performance traits related to milk production, animal growth and body size, fertility and progeny survival in 848 progeny-tested Irish Holstein-Friesian sires. Two of the four SNPs (rs42196909 and IGF2.g-3815A>G), which were in strong linkage disequilibrium (r2=0·995), were associated with milk yield (P⩽0·01) and milk protein yield (P⩽0·05); the rs42196901 SNP was also associated (P⩽0·05) with milk fat yield. Associations (P⩽0·05) with milk fat percentage and milk protein percentage were observed at the rs42196901 and IGF2.g-3815A>G SNPs, respectively. The rs42196909 and IGF2.g-3815A>G SNPs were also associated with progeny carcass conformation (P⩽0·05), while an association (P⩽0·01) with progeny carcass weight was observed at the rs42194733 SNP locus. None of the four SNPs were associated with body size, fertility and progeny survival. These findings support previous work which suggests that the IGF2 locus is an important biological regulator of milk production in dairy cattle and add to an accumulating body of research showing that imprinted genes influence many complex performance traits in cattle.

Download Full-text