scholarly journals Association of genetic variants in STAT5B gene with milk performance and mastitis related traits in dairy cattle

2021 ◽  
Vol 91 (5) ◽  
pp. 459-471
Author(s):  
Nawab Ali ◽  
◽  
Sadaf Niaz ◽  
Irfan Khattak ◽  
Naimat U. Khan ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Association Analysis ◽  
Milk Composition ◽  
Clinical Mastitis ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Exon 2 ◽  
Holstein Friesian ◽  
Hardy Weinberg Equilibrium ◽  
Milk Performance

Udder performance and health are important traits in dairy cattle worldwide. The present study aimed to explore the association of single nucleotide polymorphisms (SNPs) in the STAT5B gene in dairy cattle with milk performance and mastitis related traits. The study included 201 cows of three pure breeds (i.e. Holstein Friesian, Jersey and Achai) and two crossbred cattle at four established dairy farms in Khyber Pakhtunkhwa, Pakistan. The milk samples were analysed for somatic cell count (SCC) and milk composition (i.e. fat , protein and lactose percentages). The generalized linear model was deployed for association analysis using SAS. The pool DNA sequencing showed four (three synonymous and a 3/ UTR) SNPs in STAT5B. These SNPs were further validated in all DNA samples using SNaPshot assay. The breed-wise analysis showed that most of the SNPs were consistent with Hardy-Weinberg equilibrium (P>0.05). The association analysis revealed a significantly higher protein percentage in TT genotype and lower SCC in CC genotypes of SNP 1 (exon 2, C>T), whereas in SNP 2 (exon 16, T>C) the TT genotypes revealed significantly lower SCC and SCS compared with other genotypes (P<0.05). SNP 4 (3/ UTR, C>T) showed significantly lower SCC and frequency of clinical mastitis in the heterozygous (CT) genotype compared to the homozygous genotypes. The in silico predictions revealed changes in the RNA secondary structure for SNP 2 and SNP 3. The study suggests that STAT5B should be considered as a candidate gene, and the variants identified as useful genetic markers for improved milk composition and udder health.

scholarly journals Single nucleotide polymorphisms at the imprinted bovine insulin-like growth factor 2 (IGF2) locus are associated with dairy performance in Irish Holstein-Friesian cattle

2010 ◽  
Vol 78 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Erik W Berkowicz ◽  
David A Magee ◽  
Klaudia M Sikora ◽  
Donagh P Berry ◽  
Dawn J Howard ◽  
...  
Keyword(s):  
Body Size ◽  
Growth Factor ◽  
Single Nucleotide Polymorphisms ◽  
Dairy Cattle ◽  
Milk Production ◽  
Milk Fat ◽  
Milk Protein ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Holstein Friesian

The imprinted insulin-like growth factor 2 gene (IGF2) encodes a fetal mitogenic hormone protein (IGF-II) and has previously been shown to be associated with performance in dairy cattle. In this study we assessed genotype-phenotype associations between four single nucleotide polymorphisms (SNPs) located within the bovine IGF2 locus on chromosome 29 and a range of performance traits related to milk production, animal growth and body size, fertility and progeny survival in 848 progeny-tested Irish Holstein-Friesian sires. Two of the four SNPs (rs42196909 and IGF2.g-3815A>G), which were in strong linkage disequilibrium (r2=0·995), were associated with milk yield (P⩽0·01) and milk protein yield (P⩽0·05); the rs42196901 SNP was also associated (P⩽0·05) with milk fat yield. Associations (P⩽0·05) with milk fat percentage and milk protein percentage were observed at the rs42196901 and IGF2.g-3815A>G SNPs, respectively. The rs42196909 and IGF2.g-3815A>G SNPs were also associated with progeny carcass conformation (P⩽0·05), while an association (P⩽0·01) with progeny carcass weight was observed at the rs42194733 SNP locus. None of the four SNPs were associated with body size, fertility and progeny survival. These findings support previous work which suggests that the IGF2 locus is an important biological regulator of milk production in dairy cattle and add to an accumulating body of research showing that imprinted genes influence many complex performance traits in cattle.

scholarly journals Impact of SNPs in ACACA, SCD1, and DGAT1 Genes on Fatty Acid Profile in Bovine Milk with Regard to Lactation Phases

Animals ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 997
Author(s):  
Marzena M. Kęsek-Woźniak ◽  
Edyta Wojtas ◽  
Anna E. Zielak-Steciwko
Keyword(s):  
Milk Fat ◽  
Bovine Milk ◽  
Milk Composition ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Holstein Friesian ◽  
Dgat1 Gene ◽  
Health Promoting ◽  
Lactation Phase ◽  
Friesian Cattle

Milk fat is a dietary source of fatty acids (FA), which can be health promoting or can increase risks of some diseases. FA profile composition depends on many factors, among them gene polymorphism. This study analyzed the relation between polymorphism of acetyl-CoA carboxylase α (ACACA), stearoyl-CoA desaturase 1 (SCD1), diacylglycerol acyltransferase 1 (DGAT1) genes with FA profile in milk from Polish Holstein-Friesian cattle and determined changes of FA percentage during lactation with regard to polymorphism. Milk samples were collected twice: during the first phase of lactation (<90 Days in milk; DIM) and at the end of lactation (>210 DIM). During the first milk collection, blood samples were taken to analyze three chosen single nucleotide polymorphisms (SNPs): AJ312201.1g.1488C > G SNP in ACACA gene, A293V SNP in SCD1 gene, and K232A SNP in DGAT1 gene. Increased concentration of FA that are less beneficial for human health and have lower concentration of healthy FA in homozygotes: GG in ACACA, VV in SCD1, and KK in DGAT1 were observed, as well as a strong influence of the analyzed genes on FA with 18C atoms was also found. Moreover, it was demonstrated that lactation phase significantly affected FA percentage in milk depending on the phenotype. These results may contribute their part to knowledge toward obtaining more beneficial milk composition.

scholarly journals The Polymorphism in Various Milk Protein Genes in Polish Holstein-Friesian Dairy Cattle

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 389
Author(s):  
Magdalena Kolenda ◽  
Beata Sitkowska
Keyword(s):  
Milk Protein ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Black And White ◽  
The Past ◽  
Holstein Friesian ◽  
Milk Protein Genes ◽  
Beta Casein ◽  
Hardy Weinberg Equilibrium ◽  
Genotype And Allele Frequencies

The aim of the present study was to evaluate the genotype and allele frequencies of 24 polymorphisms in casein alpha S1 (CSN1S1), casein alpha S2 (CSN1S2), beta-casein (CSN2), kappa-casein (CSN3), and progestagen-associated endometrial protein (PAEP) genes. The study included 1900 Polish Black and White Holstein-Friesian dairy cows that were subjected to genotyping via microarrays. A total of 24 SNPs (Single Nucleotide Polymorphisms) within tested genes were investigated. Two CSN1S1 SNPs were monomorphic, while allele CSN1S1_3*G in CSN1S1_3 SNP dominated with a frequency of 99.39%. Out of seven CSN2 SNPs, four were polymorphic; however, only for CSN2_3 all three genotypes were detected. Only three out of nine SNPs within CSN3 were monomorphic. Three PAEP SNPs were also found to be polymorphic with heterozygotes being most frequent. Hardy–Weinberg equilibrium (HWE) was observed for eight variants. It was shown that only CSN3_6 was not in HWE. The fact that many of investigated SNPs were monomorphic may suggest that in the past the reproduction program favored one of these genotypes. SNPs that are included in commercially available microarrays should be monitored in relation to changes in their frequencies. If a SNP has turned monomorphic, maybe it should be considered for removal from the microarray.

scholarly journals Polymorphisms in JAK2 Gene are Associated with Production Traits and Mastitis Resistance in Dairy Cattle

2020 ◽  
Vol 20 (2) ◽  
pp. 409-423 ◽  
Author(s):  
Nawab Ali ◽  
Sadaf Niaz ◽  
Naimat Ullah Khan ◽  
Ali Gohar ◽  
Irfan Khattak ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Upstream Region ◽  
Strong Linkage Disequilibrium ◽  
Nucleotide Polymorphisms ◽  
Production Traits ◽  
Holstein Friesian ◽  
Hardy Weinberg Equilibrium ◽  
Exon 20 ◽  
Dna Pool ◽  
Lactating Dairy Cattle

AbstractThe present study was designed to investigate the effects of single nucleotide polymorphisms (SNPs) in the JAK2 gene on the production and mastitis related traits in dairy cattle. Blood and milk samples were collected from 201 lactating dairy cattle of three breeds, i.e. Holstein Friesian (HF), Jersey (J) and Achai (A) and their crosses maintained at well-established dairy farms in Khyber Pakhtunkhwa, Pakistan. Generalized linear model was used to evaluate the association between genotypes and the studied traits. A DNA pool was made from randomly selected 30 samples which revealed three SNPs, i.e. SNP 1 in 5’ upstream region (G>A, rs379754157), SNP 2 in intron 15 (A>G, rs134192265), and SNP 3 in exon 20 (A>G, rs110298451) that were further validated in the population under study using SNaPshot technique. Of the three SNPs, SNP 1 did not obey Hardy-Weinberg equilibrium (P<0.05). SNP 2 and SNP 3 were found to be in strong linkage disequilibrium and allele G was highly prevalent compared to allele A in these SNPs. in SNP 1, the GG genotype was associated with significantly (P<0.01) higher SCC, whereas SNP 2 and SNP 3 were significantly (P<0.01) associated with higher lactose percentage compared to the other geno-types. The haplogroups association analysis revealed that H1H2 (GG GG AG) has significantly lower SCC than H2H2 (GG GG GG). The results infer that JAK2 could be an important candidate gene and the studied SNPs might be useful genetic markers for production and mastitis related traits.

Lactoferrin gene variants, their expression in the udder and mastitis susceptibility in dairy cattle

2015 ◽  
Vol 55 (8) ◽  
pp. 999 ◽  
Author(s):  
Adrianna Pawlik ◽  
Grażyna Sender ◽  
Magdalena Sobczyńska ◽  
Agnieszka Korwin-Kossakowska ◽  
Henryka Lassa ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Holstein Friesian ◽  
Lactoferrin Gene ◽  
Estimated Breeding Values ◽  
Mastitis Susceptibility

Lactoferrin gene (LF) is regarded as one of the potential markers of mastitis susceptibility/resistance in dairy cattle. The study’s aim was therefore, to investigate the feasibility of two single nucleotide polymorphisms (SNP), placed in the 5′-flanking region and 3′-untranslated region of the LF gene, to serve as mastitis markers. The associations between these SNP and the expression of LF, both on mRNA and protein level, were estimated in the milk of Polish Holstein-Friesian cows. The relationships between polymorphisms and cows’ estimated breeding values (EBV) for somatic cell count were also calculated. It was shown that both polymorphisms have a significant impact on lactoferrin content in milk, and that LF+32 SNP is associated with the cow’s EBV for somatic cell count. No association between SNP chosen for the study and lactoferrin mRNA abundance in milk somatic cells was observed. We propose LF+32 SNP for a molecular marker of mastitis resistance in dairy cows.

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