Effect of plant derived epinephrine on Saccharomyces cerevisiae

2021 ◽  
Vol 16 (10) ◽  
pp. 9-12
Author(s):  
Sandhya Menon ◽  
Jessy Pius ◽  
Sachin Palekar ◽  
Nandini G.
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Adenylate Cyclase ◽  
G Protein ◽  
Plant Species ◽  
Yeast Cells ◽  
G Protein Coupled Receptor ◽  
Hepatic Cells ◽  
Animal System ◽  
Fight Or Flight Response ◽  
G Protein Coupled

Epinephrine, a hormone known to produce ‘fight or flight’ response in higher animals, stimulates the hepatic cells to release stored glucose. The receptor for epinephrine is known to be a G-protein coupled receptor (GPCR). Baker’s yeast, also known as Saccharomyces cerevisiae, is reported to have a Gprotein coupled receptor (GPCR). The G-protein coupled receptor (GPCR) has a role in sensing glucose activation of adenylate cyclase during the switch from respirative/gluconeogenic metabolism to fermentation. Epinephrine, having varying roles in the animal system, has been reported in certain plant species. In the present study, Epinephrine quantified from extracts of G. globosa and P. oleracea was evaluated for its effect on the yeast cells.

scholarly journals Affinity purification and characterization of a G-protein coupled receptor, Saccharomyces cerevisiae Ste2p

2007 ◽  
Vol 56 (1) ◽  
pp. 62-71 ◽  
Author(s):  
Byung-Kwon Lee ◽  
Kyung-Sik Jung ◽  
Cagdas Son ◽  
Heejung Kim ◽  
Nathan C. VerBerkmoes ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
G Protein ◽  
Affinity Purification ◽  
G Protein Coupled Receptor ◽  
Purification And Characterization ◽  
G Protein Coupled

Carbon source induced yeast-to-hypha transition in Candida albicans is dependent on the presence of amino acids and on the G-protein-coupled receptor Gpr1

2005 ◽  
Vol 33 (1) ◽  
pp. 291-293 ◽  
Author(s):  
M.M. Maidan ◽  
J.M. Thevelein ◽  
P. Van Dijck
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acids ◽  
Candida Albicans ◽  
Carbon Source ◽  
G Protein ◽  
High Glucose ◽  
Glucose Concentration ◽  
G Protein Coupled Receptor ◽  
Hypha Formation ◽  
G Protein Coupled

Yeast-to-hypha transition in Candida albicans can be induced by a wide variety of factors, including specific nutrients. We have started to investigate the mechanism by which some of these nutrients may be sensed. The G-protein-coupled receptor Gpr1 is required for yeast-to-hypha transition on various solid hypha-inducing media. Recently we have shown induction of Gpr1 internalization by specific amino acids, e.g. methionine. This suggests a possible role for methionine as a ligand of CaGpr1. Here we show that there is a big variation in methionine-induced hypha formation depending on the type of carbon source present in the medium. In addition high glucose concentrations repress hypha formation whereas a concentration of 0.1%, which mimics the glucose concentration present in the bloodstream, results in maximal hypha formation. Hence, it remains unclear whether Gpr1 senses sugars, as in Saccharomyces cerevisiae, or specific amino acids like methionine.

scholarly journals Molecular cloning of an orphan G-protein-coupled receptor that constitutively activates adenylate cyclase

1995 ◽  
Vol 309 (3) ◽  
pp. 837-843 ◽  
Author(s):  
D Eggerickx ◽  
J F Denef ◽  
O Labbe ◽  
Y Hayashi ◽  
S Refetoff ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
G Protein ◽  
Rnase Protection Assay ◽  
Biological Significance ◽  
Orphan Receptor ◽  
Constitutive Activity ◽  
G Protein Coupled Receptor ◽  
Rnase Protection ◽  
G Protein Coupled ◽  
Stimulation Of

A human gene encoding an orphan G-protein-coupled receptor named ACCA (adenylate cyclase constitutive activator) was isolated from a genomic library using as a probe a DNA fragment obtained by low-stringency PCR. Human ACCA (hACCA) is a protein of 330 amino acids that exhibits all the structural hallmarks of the main family of G-protein-coupled receptors. Expression of hACCA resulted in a dramatic stimulation of adenylate cyclase, similar in amplitude to that obtained with other Gs-coupled receptors fully activated by their respective ligands. This stimulation was obtained in a large variety of stable cell lines derived from various organs, and originating from different mammalian species. hACCA was found to be the human homologue of a recently reported mouse orphan receptor (GPCR21). The mouse ACCA (mACCA) was therefore recloned by PCR, and expression of mACCA in Cos-7 cells demonstrated that the mouse receptor behaved similarly as a constitutive activator of adenylate cyclase. It is not known presently whether the stimulation of adenylate cyclase is the result of a true constitutive activity of the receptor or, alternatively, is the consequence of a permanent stimulation by a ubiquitous ligand. The tissue distribution of mACCA was determined by RNase protection assay. Abundant transcripts were found in the brain, whereas lower amounts were detected in testis, ovary and eye. Various hypotheses concerning the constitutive activity of ACCA and their potential biological significance are discussed.

scholarly journals Specific  -Arrestins Negatively Regulate Saccharomyces cerevisiae Pheromone Response by Down-Modulating the G-Protein-Coupled Receptor Ste2

2014 ◽  
Vol 34 (14) ◽  
pp. 2660-2681 ◽  
Author(s):  
C. G. Alvaro ◽  
A. F. O'Donnell ◽  
D. C. Prosser ◽  
A. A. Augustine ◽  
A. Goldman ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
G Protein ◽  
Pheromone Response ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled
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