Xylanase production by halophilic bacterium Gracilibacillus sp. TSCPVG under solid state fermentation

2021 ◽  
Vol 16 (7) ◽  
pp. 92-100
Author(s):  
Giridhar Poosarla Venkata ◽  
T.S. Chandra
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Substantial Reduction ◽  
Nitrogen Sources ◽  
Halophilic Bacterium ◽  
Fermentation Medium ◽  
Solid Substrate ◽  
Xylanase Production ◽  
Hemicellulose Degradation ◽  
Huge Impact

We previously demonstrated the production and purification of novel halo-acid-alkali-thermo-stable xylanase from halophilic bacterium Gracilibacillus sp. TSCPVG using submerged fermentation (SmF). In this work, we further showed the production of halophilic xylanase by solid state fermentation (SSF) from halophilic bacterium Gracilibacillus sp. TSCPVG using cheap solid-substrate wheat bran. High levels of enzyme titers (657 U/g dw) were obtained after varying certain SSF parameters such as incubation period, particle size, moisture ratio, salinity, pH, temperature and by supplementing different carbon and nitrogen sources externally to the fermentation medium. This bacterium could be a novel strain due to its ability to produce high titers of xylanase with extremophilic characters even under the conditions of SSF. Thus, this beneficiary role may lead to a substantial reduction in the overall cost of enzyme production and may have a huge impact on the economics of hemicellulose degradation under high saline conditions.

Efficient Production of Laccase from Armillariella tabescens by Solid State Fermentation

2014 ◽  
Vol 1010-1012 ◽  
pp. 42-47
Author(s):  
Jun Yao He ◽  
Xuan Yi Ye ◽  
Qing Zhi Ling ◽  
Li Hui Dong
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Nitrogen Source ◽  
Solid State Fermentation ◽  
Initial Moisture Content ◽  
Nitrogen Sources ◽  
Solid Substrate ◽  
Laccase Production ◽  
Efficient Production ◽  
Operational Variables

The production of laccase by solid-state fermentation (SSF) usingArmillariella tabescenswas studied. Wheat bran was selected to be the most suitable solid substrate. Several operational variables including nitrogen source, moisture content, copper and aromatic inducers were investigated. The results showed that the complex nitrogen sources, NH4NO3coupled with peptone was shown to be the best nitrogen source. 75% of initial moisture content was proved to be appropriate. Copper significantly influenced the laccase production and the yield of laccase was improved by addition of 1.5 mM copper sulphate in the medium. Guaiacol efficiently induced the laccase production and the enzyme yield (24500U/g) was enhanced by 32% compared with he control without any aromatic inducers. Efficient production of laccase fromA. tabescenscan be achieved by solid-state fermentation.

scholarly journals Solid-state fermentation for production of Pectic enzymes by Aspergillus niger

2017 ◽  
Vol 7 (5) ◽  
pp. 17
Author(s):  
Mirza M.V. Baig ◽  
Aniruddha Ratnakar Apastambh
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Nitrogen Source ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Solid Substrate ◽  
Inorganic Nitrogen Source ◽  
Pectic Enzymes

The production of Pectic enzymes by Aspergillus niger was studied under solid state fermentation (SSF). The effect of fermentation condition such as substrate concentration, inoculum volume, incubation time, moistening agent, inducers and organic and inorganic nitrogen sources was studied for enzyme production. Culture conditions were optimized for maximal yield of enzyme. The solid substrate wheat bran was most suitable for pectic enzyme production under SSF. Enzyme production was found maximum after 10 days of incubation. Lactose was found to be most effective as inducer. Gelatin as organic nitrogen source and ammonium nitrate as inorganic nitrogen source yielded high enzyme titres.

scholarly journals Isolation, screening of Aspergillus flavus and its production parameters for á- amylase under solid state fermentation

2011 ◽  
Vol 3 (2) ◽  
pp. 268-273
Author(s):  
K. N. Geetha ◽  
K. Jeyaprakash ◽  
Y. P. Nagaraja
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Incubation Temperature ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Soluble Starch ◽  
Solid Substrate ◽  
Fungal Species

The amylase producing fungi were isolated from spoiled fruits, vegetables and soil, in and around Bangalore, Karnataka, India. The isolates were identified and five fungal species were screened. The best amylase producer among them, Aspergillus sp was selected for enzyme production by both sub merged fermentation using mineral salt medium (MSM) and solid state fermentations using wheat bran as a solid substrate. The various parameters influencing solid state fermentation were optimized. The most important factors are such as pH, incubation temperature, incubation period, carbon sources, nitrogen sources and moisture content. The maximum amount of enzyme production was obtained when solid state fermentation was carried out with soluble starch as carbon source and beef extract (1% each) as nitrogen source, optimum conditions of pH 7.0, an incubation temperature of 25 (±2) °C, incubation time 96 h and 62% moisture content.

scholarly journals Erratum to: Optimization of Xylanase Production by Filamentous Fungi in Solid-State Fermentation and Scale-up to Horizontal Tube Bioreactor

2015 ◽  
Vol 176 (1) ◽  
pp. 317-319 ◽  
Author(s):  
N. Pérez-Rodríguez ◽  
F. Oliveira ◽  
B. Pérez-Bibbins ◽  
I. Belo ◽  
A. Torrado Agrasar ◽  
...  
Keyword(s):  
Solid State ◽  
Filamentous Fungi ◽  
Solid State Fermentation ◽  
Scale Up ◽  
Horizontal Tube ◽  
Xylanase Production
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