Improvement of cellulase and xylanase production in Penicillium oxalicum under solid-state fermentation by flippase recombination enzyme/ recognition target-mediated genetic engineering of transcription repressors

2021 ◽  
pp. 125366
Author(s):  
Ying-Ying Lin ◽  
Shuai Zhao ◽  
Xiong Lin ◽  
Ting Zhang ◽  
Cheng-Xi Li ◽  
...  
Keyword(s):  
Solid State ◽  
Genetic Engineering ◽  
Solid State Fermentation ◽  
Penicillium Oxalicum ◽  
Xylanase Production ◽  
Enzyme Recognition ◽  
Transcription Repressors

scholarly journals Transcription Factor Atf1 Regulates Expression of Cellulase and Xylanase Genes during Solid-State Fermentation of Ascomycetes

2019 ◽  
Vol 85 (24) ◽  
Author(s):  
Shuai Zhao ◽  
Xu-Zhong Liao ◽  
Jiu-Xiang Wang ◽  
Yuan-Ni Ning ◽  
Cheng-Xi Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Solid State ◽  
Rna Sequencing ◽  
Solid State Fermentation ◽  
Carbon Sources ◽  
Penicillium Oxalicum ◽  
Xylanase Production ◽  
Content Type ◽  
Ascomycete Fungi

ABSTRACT Transcriptional regulation of cellulolytic and xylolytic genes in ascomycete fungi is controlled by specific carbon sources in different external environments. Here, comparative transcriptomic analyses of Penicillium oxalicum grown on wheat bran (WB), WB plus rice straw (WR), or WB plus Avicel (WA) as the sole carbon source under solid-state fermentation (SSF) revealed that most of the differentially expressed genes (DEGs) were involved in metabolism, specifically, carbohydrate metabolism. Of the DEGs, the basic core carbohydrate-active enzyme-encoding genes which responded to the plant biomass resources were identified in P. oxalicum, and their transcriptional levels changed to various extents depending on the different carbon sources. Moreover, this study found that three deletion mutants of genes encoding putative transcription factors showed significant alterations in filter paper cellulase production compared with that of a parental P. oxalicum strain with a deletion of Ku70 (ΔPoxKu70 strain) when grown on WR under SSF. Importantly, the ΔPoxAtf1 mutant (with a deletion of P. oxalicum Atf1, also called POX03016) displayed 46.1 to 183.2% more cellulase and xylanase production than a ΔPoxKu70 mutant after 2 days of growth on WR. RNA sequencing and quantitative reverse transcription-PCR revealed that PoxAtf1 dynamically regulated the expression of major cellulase and xylanase genes under SSF. PoxAtf1 bound to the promoter regions of the key cellulase and xylanase genes in vitro. This study provides novel insights into the regulatory mechanism of fungal cellulase and xylanase gene expression under SSF. IMPORTANCE The transition to a more environmentally friendly economy encourages studies involving the high-value-added utilization of lignocellulosic biomass. Solid-state fermentation (SSF), that simulates the natural habitat of soil microorganisms, is used for a variety of applications such as biomass biorefinery. Prior to the current study, our understanding of genome-wide gene expression and of the regulation of gene expression of lignocellulose-degrading enzymes in ascomycete fungi during SSF was limited. Here, we employed RNA sequencing and genetic analyses to investigate transcriptomes of Penicillium oxalicum strain EU2101 cultured on medium containing different carbon sources and to identify and characterize transcription factors for regulating the expression of cellulase and xylanase genes during SSF. The results generated will provide novel insights into genetic engineering of filamentous fungi to further increase enzyme production.

scholarly journals Erratum to: Optimization of Xylanase Production by Filamentous Fungi in Solid-State Fermentation and Scale-up to Horizontal Tube Bioreactor

2015 ◽  
Vol 176 (1) ◽  
pp. 317-319 ◽  
Author(s):  
N. Pérez-Rodríguez ◽  
F. Oliveira ◽  
B. Pérez-Bibbins ◽  
I. Belo ◽  
A. Torrado Agrasar ◽  
...  
Keyword(s):  
Solid State ◽  
Filamentous Fungi ◽  
Solid State Fermentation ◽  
Scale Up ◽  
Horizontal Tube ◽  
Xylanase Production

Xylanase Production by Penicillium canescens on Soya Oil Cake in Solid-State Fermentation

2009 ◽  
Vol 160 (1) ◽  
pp. 50-62 ◽  
Author(s):  
Assamoi Allah Antoine ◽  
Destain Jacqueline ◽  
Philippe Thonart
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Penicillium Canescens ◽  
Xylanase Production ◽  
Soya Oil

High-level of xylanase production by the thermophilic Paecilomyces themophila J18 on wheat straw in solid-state fermentation

2006 ◽  
Vol 97 (15) ◽  
pp. 1794-1800 ◽  
Author(s):  
S.Q. Yang ◽  
Q.J. Yan ◽  
Z.Q. Jiang ◽  
L.T. Li ◽  
H.M. Tian ◽  
...  
Keyword(s):  
Solid State ◽  
Wheat Straw ◽  
Solid State Fermentation ◽  
Xylanase Production ◽  
High Level

scholarly journals Optimization of Xylanase Production by Filamentous Fungi in Solid-State Fermentation and Scale-up to Horizontal Tube Bioreactor

2014 ◽  
Vol 173 (3) ◽  
pp. 803-825 ◽  
Author(s):  
N. Pérez-Rodríguez ◽  
F. Oliveira ◽  
B. Pérez-Bibbins ◽  
I. Belo ◽  
A. Torrado Agrasar ◽  
...  
Keyword(s):  
Solid State ◽  
Filamentous Fungi ◽  
Solid State Fermentation ◽  
Scale Up ◽  
Horizontal Tube ◽  
Xylanase Production
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