A clinical protocol for isolating human preantral follicles using a good manufacturing practices grade collagenase NB6

Author(s):  
Elodie Mouloungui
Planta Medica ◽  
2008 ◽  
Vol 74 (03) ◽  
Author(s):  
D Fabricant ◽  
V Whitsitt ◽  
C Reynolds ◽  
C Beehner ◽  
A Secrist ◽  
...  

2003 ◽  
Vol 1 (2) ◽  
pp. 198-207
Author(s):  
H. Mabel Preloran ◽  
Silvia Balzano

This paper explores the emotional world of a recent Mexican immigrant who lives in Los Angeles and is awaiting the results of the amniocentesis she has ambivalently agreed to. She is 45 years old and has given birth to two children with severe disabilities and two who are apparently normal. We focus our analysis on the woman's reactions and feelings during the nine days she spends waiting for the test results. We show that the standard prenatal genetic clinical protocol aimed at providing medical education and requiring professional neutrality and emotional detachment left the woman feeling rejected and subsequently unwilling to seek information or support from her clinicians. We find that while the intent of a protocol of neutrality is to enable patients to make informed decisions without feeling pressure from clinicians, some women want greater emotional engagement. We argue that professional neutrality can inhibit patient-clinician communication, hamper medical education, and ultimately detract from patients' ability to make informed medical choices. / El presente artículo explora el mundo emocional de Rocío, una inmigrante mexicana, quien se encuentra esperando los resultados de una amniocentesis que aceptó hacerse, a pesar de las dudas sobre la credibilidad y utilidad de la misma. Rocío, de 45 años, tenía ya otros hijos, dos con anormalidades severas y dos aparentemente sanos. Centramos nuestro análisis en los sentimientos y reacciones durante los nueve días que transcurren mientras espera el diagnóstico. En este trabajo mostramos cómo la forma de presentar la información médica puede llegar a entorpecer la toma de decisión de un paciente. El protocolo genético tiene por meta proveer información médica manteniendo una cierta distancia profesional y emocional. Estas condiciones hacen que, en nuestro estudio de caso, la paciente se sienta rechazada y sin deseos de acercarse al personal médico, ya sea en busca de apoyo emocional o información que aclararía sus dudas. Creemos que, mientras el objetivo de la neutralidad profesional es asegurar que el paciente decida con los conocimientos adecuados y, a la vez, sin sentirse presionado, algunas mujeres preferirían un mayor acercamiento emocional por parte del personal médico cuando deben decidir sobre pruebas o tratamientos. Creemos que la neutralidad profesional puede llegar a inhibir la comunicación médico-paciente, dificultar la comprensión de la información y, por último, obstaculizar la habilidad de tomar decisiones informadas por parte de los pacientes.


Reproduction ◽  
2000 ◽  
pp. 375-385 ◽  
Author(s):  
K Sundfeldt ◽  
Y Piontkewitz ◽  
H Billig ◽  
L Hedin

The cadherins and their cytoplasmic counterparts, the catenins, form the adherens junctions, which are of importance for tissue integrity and barrier functions. The development and maturation of the ovarian follicle is characterized by structural changes, which require altered expression or function of the components involved in cell-cell contacts. The present study examined the cell-specific localization and temporal expression of epithelial cadherin (E-cadherin) and alpha- and beta-catenin during follicular development, ovulation and corpus luteum formation in the immature gonadotrophin- and oestrogen-stimulated rat ovary. Immunohistochemistry and immunoblotting demonstrated the expression of E-cadherin in theca and interstitial cells of immature ovaries before and after injection of equine chorionic gonadotrophin (eCG). E-cadherin was not detected in granulosa cells, except in the preantral follicles located to the inner region of the ovary. The content of E-cadherin in theca and interstitial cells decreased after an ovulatory dose of hCG. Granulosa cells of apoptotic follicles did not express E-cadherin. Oestrogen treatment (diethylstilboestrol) of immature rats for up to 3 days did not result in a measurable expression of E-cadherin in granulosa cells. alpha- and beta-catenin were expressed in all ovarian compartments. The concentration of beta-catenin was constant during the follicular phase, whereas the content of alpha-catenin decreased in granulosa cells after treatment with diethylstilboestrol or hCG. The expression of alpha-catenin was also reduced in theca and interstitial cells after hCG. alpha- and beta-catenin were present in most ovarian cells at all stages of folliculogenesis. Therefore, the catenins have the potential to associate with different members of the cadherin family and to participate in the regulation of cytoskeletal structures and intracellular signalling. The restricted expression of E-cadherin in granulosa cells of preantral follicles indicates a role in the recruitment of these follicles to subsequent cycles. The specific decrease of alpha-catenin in granulosa cells and the reduction of both alpha-catenin and E-cadherin in theca cells of ovulatory follicles might reflect some of the molecular changes in cell-cell adhesion associated with ovulation and luteinization.


Reproduction ◽  
2000 ◽  
pp. 221-228 ◽  
Author(s):  
HF Irving-Rodgers ◽  
RJ Rodgers

Different morphological phenotypes of follicular basal lamina and of membrana granulosa have been observed. Ten preantral follicles (< 0. 1 mm), and 17 healthy and six atretic antral follicles (0.5-12 mm in diameter) were processed for light and electron microscopy to investigate the relationship the between follicular basal lamina and membrana granulosa. Within each antral follicle, the shape of the basal cells of the membrana granulosa was uniform, and either rounded or columnar. There were equal proportions of follicles </= 4 mm in diameter with columnar basal cells and with rounded basal cells. Larger follicles had only rounded basal cells. Conventional basal laminae of a single layer adjacent to the basal granulosa cells were observed in healthy follicles at the preantral and antral stages. However, at the preantral stage, the conventional types of basal lamina were enlarged or even partially laminated. A second type of basal lamina, described as 'loopy', occurred in about half the preantral follicles and in half the antral follicles </= 4 mm diameter. 'Loopy' basal laminae were not observed in larger follicles. 'Loopy' basal laminae were composed of basal laminae aligning the basal surface of basal granulosa cells, but with additional layers or loops often branching from the innermost layer. Each loop was usually < 1 microm long and had vesicles (20-30 nm) attached to the inner aspect. Basal cellular processes were also common, and vesicles could be seen budding off from these processes. In antral follicles, conventional basal laminae occurred in follicles with rounded basal granulosa cells. Other follicles with columnar cells, and atretic follicles, had the 'loopy' basal lamina phenotype. Thus, follicles have different basal laminae that relate to the morphology of the membrana granulosa.


2019 ◽  
Vol 15 (68) ◽  
pp. 091
Author(s):  
V. D. Pariy ◽  
S. M. Hryshchuk ◽  
N. M. Korneychuk ◽  
A. M. Harlinska ◽  
L. V. Horokhova

Author(s):  
Dr. Akash S Changole ◽  
Mandip Goyal ◽  
Harish CR

Background: Quality control and the standardization of herbal medicines involve steps like standard source and quality of raw materials, good manufacturing practices and adequate analytical screening. These practices play a vital role in guaranting the quality and stability of herbal preparations. Chandrashakaladi Vataka is an Ayurvedic herbal formulation mentioned to be beneficial in Kushtha. Till date no published data is available on its analytical profile. Aim: To develop the Pharmacognostical and Phyto-chemical profile of Chandrashakaladi Vataka. Material and Methods: Chandrashakaladi Vataka was prepared as per classical method and analytical findings were recorded. Samples were subjected to organoleptic analysis, physico-chemical analysis and HPTLC examination by optimizing the solvent systems. Results and Conclusions: Pharmacognostical profile of Chandrashakaladi Vataka was established. Loss on drying, Ash value, Acid insoluble extract, Methanol soluble extract, Chandrashakaladi Vataka was found within prescribed limits. HPTLC profile of Chandrashakaladi Vataka revealed 12 spots at 254 nm and 13 spots at 366 nm.


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