04 / Morphokinetic analysis of euploid blastocysts: searching for non-invasive criteria of embryo implantation additional to chromosomal assessment.

2018 ◽  
Author(s):  
Roberta Maggiulli ◽  
Danilo Cimadomo ◽  
Laura Francesca Rienzi
Keyword(s):  
Embryo Implantation ◽  
Non Invasive

scholarly journals Is day 5 blastocyst better than day 6 blastocyst? Evidence from NGS-based PGT-A results

2021 ◽  
Author(s):  
Ting Zhang
Keyword(s):  
Maternal Age ◽  
Embryo Implantation ◽  
Trophectoderm Biopsy ◽  
Preimplantation Genetic Testing ◽  
Non Invasive ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing ◽  
Ploidy Status ◽  
Better Than ◽  
Euploid Embryo

Abstract Background Aneuploidy is the principal genetic factor leading to the failure of embryo implantation. For most patients who accept the non-preimplantation genetic testing (PGT) cycle, non-invasive methods to select euploid embryos with the best pregnancy potential are desirable Methods This retrospective study recruited women undergoing PGT for aneuploidy (PGT-A) with trophectoderm biopsy from January 2019 to December 2020. The ploidy status of embryos was determined by next generation sequencing (NGS). Results Altogether 2531 blastocysts from 839 PGT-A cycles were evaluated. The euploid rate of day 5 blastocysts seemed to be significantly higher than that of day 6 blastocysts, either from the same ovarian stimulation (OS) cycles (49.9% vs 35.7%, P < 0.001) or from different OS cycles (48.2% vs 27.8%, P < 0.001). Both the younger maternal age (adjusted OR = 0.917, 95% CI: 0.892–0.944, P < 0.001) and day 5 stage (adjusted OR = 1.735, 95% CI: 1.415–2.127, P < 0.001) were independently associated with the greater euploid rate of blastocysts. However, after single euploid embryo transfer, the clinical outcomes of day 5 blastocysts were comparable to those of day 6 blastocysts, no matter whether they were from the same OS cycles or not. Conclusions Our results revealed that day 5 blastocysts possess a higher euploid rate than day 6 blastocysts independent of the OS cycles. Giving priority to a day 5 blastocyst over day 6 blastocyst will increase the likelihood to select single euploid embryo for transfer in non-PGT cycles.

scholarly journals Performance evaluation of microbead and ELISA assays for follicular G-CSF: a non-invasive biomarker of oocyte developmental competence for embryo implantation

2010 ◽  
Vol 86 (2) ◽  
pp. 126-132 ◽  
Author(s):  
Nathalie Lédée ◽  
Carine Munaut ◽  
Valérie Sérazin ◽  
Sophie Perrier d’Hauterive ◽  
Letizia Lombardelli ◽  
...  
Keyword(s):  
Performance Evaluation ◽  
Embryo Implantation ◽  
Developmental Competence ◽  
Non Invasive ◽  
Oocyte Developmental Competence

scholarly journals Non-invasive intra-uterine administration of Botulinum Toxin A enhances endometrial angiogenesis and improves the rates of embryo implantation

2020 ◽  
Author(s):  
Hwa Seon Koo ◽  
Min-Ji Yoon ◽  
Seon-Hwa Hong ◽  
Jungho Ahn ◽  
Hwijae Cha ◽  
...  
Keyword(s):  
Botulinum Toxin ◽  
Botulinum Toxin A ◽  
Embryo Implantation ◽  
Endometrial Receptivity ◽  
Marker Genes ◽  
Implantation Failure ◽  
Toxin A ◽  
Major Barrier ◽  
Repeated Implantation Failure ◽  
Non Invasive

Abstract Background. Endometrial angiogenesis plays crucial roles in determining the endometrial receptivity. Defects in endometrial receptivity often cause repeated implantation failure, which is one of the major unmet needs for infertility and contributes a major barrier to the assisted reproductive technology. Despite the numerous extensive research work, there are currently no effective evidence-based treatments to prevent or cure this condition.Method. As a non-invasive treatment strategy, Botulinum toxin A (BoTA) was administered into one side of mouse uterine horns and saline was infused into the other side of horns for the control. Impact of BoTA was assessed in the endometrium at 3 or 8 days after infusion.Results. We demonstrated that BoTA administration enhances the capacity of endothelial cell tube formation and sprouting. The intrauterine BoTA administration significantly induced endometrial angiogenesis displaying increased numbers of vessel formation and expression levels of related-marker genes. Moreover, BoTA intrauterine application promoted the endometrial receptivity and the rates of embryo implantation were improved with BoTA treatment with no morphologically retarded embryos. Conclusion. Intrauterine BoTA treatment has a beneficial effect on vascular reconstruction of functional endometrium prior to embryo implantation by increasing endometrial blood flow near the uterine cavity suggesting BoTA treatment as a potential therapeutic strategy for patients who are suffering from repeated implantation failure with the problems with endometrial receptivity.

scholarly journals Bioengineering in women's health, volume 2: pregnancy—from implantation to parturition

2019 ◽  
Vol 9 (5) ◽  
pp. 20190081
Author(s):  
Kristin S. Miller ◽  
Kristin Myers ◽  
Michelle Oyen
Keyword(s):  
Women’S Health ◽  
Women's Health ◽  
Embryo Implantation ◽  
Computational Modelling ◽  
Microfluidic Devices ◽  
Diagnostic Techniques ◽  
Biological Processes ◽  
Special Issue ◽  
Non Invasive ◽  
Dynamic Time

This special issue of Interface Focus is the second of two sets of articles on the topic of bioengineering in women's health. This second issue in the series focuses on pregnancy, a dynamic time in a women's life that involves dramatic physiologic changes within a relatively small timeframe. Pregnancy demands endurance and resilience of one's body and represents a critical component of women's health research. The health of an individual leading up to, during and after pregnancy is paramount for reproductive health and the lifelong health of offspring. The articles in this issue explore physiological events that support reproduction spanning from embryo implantation, through gestation, to delivery and parturition. Specifically, the articles highlight essential developments in placenta, fetal membranes, cervix, pelvic floor and anthropometry research. The featured bioengineering disciplines deployed to study such complex biological processes are diverse, with articles detailing the latest advancements in computational modelling at various biological length-scales, biomaterial design, material modelling, non-invasive diagnostic techniques, microfluidic devices and experimental mechanics. This second issue continues the first in this series, on the physiology of the non-pregnant woman.

Effect of Systemic Cortisol on Pregnancy Rate in Repeat Breeding Cows during Early Pregnancy

2021 ◽  
Author(s):  
K. Rajamanickam ◽  
M. Sameer Ali ◽  
V. Leela
Keyword(s):  
Pregnancy Rate ◽  
Early Pregnancy ◽  
Embryo Implantation ◽  
Positive Association ◽  
Serum Cortisol ◽  
Urinary Cortisol ◽  
Non Invasive ◽  
Hormonal Balance ◽  
Cortisol Levels ◽  
Pituitary Adrenal Axis

Background: The effect of systemic cortisol on pregnancy rate during early pregnancy in repeat breeding cows was estimated.Methods: Oestrus synchronisation was done in 20 repeat breeders and samples were collected on different days of post-insemination to estimate cortisol.Result: Trans-rectal ultrasonography on 26th day of post insemination revealed a pregnancy rate of 45%. When two groups were compared, serum, salivary and urinary cortisol level of non-pregnant animals were significantly (P less than 0.05) increased than that of pregnant animals on different days of post-insemination. Within non-pregnant animals, serum, salivary and urinary cortisol levels showed a significant (P less than 0.05) variation between different days of post insemination, but this variation was not observed in pregnant animals. Spearman rho correlation revealed positive association (P less than 0.05) of systemic cortisol with pregnancy rate. In non-pregnant animals, salivary and urinary cortisol levels were observed to be positively correlated (P less than 0.05) with serum cortisol. The results indicate that systemic cortisol has influence on pregnancy rate in repeat breeding cows, this may be due to its effect on embryo implantation and hormonal balance, which requires further validation. Association of salivary and urinary cortisol with serum cortisol indicates their use as non-invasive samples to monitor the hypothalamic-pituitary-adrenal axis activity in repeat breeders.

scholarly journals Embryo-Derived Chemokine CXCL12 Enhances Pregnancy Outcome via Improvement of Endometrial Receptivity

2020 ◽  
Author(s):  
Hwa Seon Koo ◽  
Min-Ji Yoon ◽  
Seon-Hwa Hong ◽  
Jungho Ahn ◽  
Hwijae Cha ◽  
...  
Keyword(s):  
Embryo Implantation ◽  
Research Work ◽  
Endometrial Receptivity ◽  
Invasive Treatment ◽  
Vessel Formation ◽  
Non Invasive ◽  
Evidence Based Treatments ◽  
Chemokine Cxcl12

Abstract Background: Successful pregnancy inevitably depends on the implantation of a competent embryo into a receptive endometrium. Although a remarkable improvement of assisted reproductive technology (ART) has been achieved over the last few decades, there are still a number of infertile women experiencing frequent ART failure after repeated attempts due to many unsolved problems including repeated failure of implantation. Many substances have been suggested to improve the rates of embryo implantation by enhancing the endometrial receptivity for the patients who are suffering from repeated failure of implantation. However, despite these numerous extensive research work, there are currently no effective evidence-based treatments to prevent or cure this condition. Therefore, here we aim to suggest non-invasive intra-uterine administration of embryo-secreted chemokine CXCL12 as an effective therapeutic intervention to solve this problem.Results: We demonstrated that chemokine CXCL12 is derived from pre- and peri-implanting embryos and its interaction with endometrial CXCR4 and CXCR7 enhances endometrial receptivity and significantly promoted endothelial vessel formation and sprouting in vitro. Consistently, intra-uterine CXCL12 administration in vivo, which is a completely non-invasive treatment strategy, improved endometrial receptivity showing increased integrin b3 and its ligand osteopontin, and induced endometrial angiogenesis displaying increased numbers of vessel formation near the lining of endometrial epithelial layer with higher CD31 and CD34 expression. Furthermore, intra-uterine CXCL12 application dramatically promoted the rates of embryo implantation with no morphologically retarded embryos. Conclusions: Our present study provides a novel evidence that improved uterine endometrial receptivity and enhanced angiogenesis induced by embryo-derived chemokine CXCL12 may aid to develop a non-invasive therapeutic strategy for clinical treatment or supplement for the patients with repeated implantation failure with less risk.

VALIDATION OF A NON-INVASIVE METABOLOMICS METHOD TO PREDICT EMBRYO IMPLANTATION

2021 ◽  
Vol 116 (3) ◽  
pp. e172
Author(s):  
Sara Cabello-Pinedo ◽  
Hussain A.N. Abdulla ◽  
Ane Becerril-Iraola ◽  
Maria Escriba ◽  
Juana Crespo ◽  
...  
Keyword(s):  
Embryo Implantation ◽  
Non Invasive
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