COMPARISON OF FROZEN SECTION WITH PARAFFIN BLOCK PATHOLOGICAL EXAMINATION RESULTS IN PATIENTS WITH GYNECOLOGICAL MALIGNANCIES AT PROF. DR. R.D. KANDOU GENERAL HOSPITAL

2021 ◽  
Author(s):  
Hanna Febry Imelda Poluan ◽  
Laihad Bismarck ◽  
Frank Mitchell Marvel Wagey
Keyword(s):  
General Hospital ◽  
Frozen Section ◽  
Pathological Examination ◽  
Gynecological Malignancies ◽  
Paraffin Block

scholarly journals Real-time intraoperative glioma diagnosis using fluorescence imaging and deep convolutional neural networks

2021 ◽  
Author(s):  
Biluo Shen ◽  
Zhe Zhang ◽  
Xiaojing Shi ◽  
Caiguang Cao ◽  
Zeyu Zhang ◽  
...  
Keyword(s):  
Neural Networks ◽  
Real Time ◽  
Fluorescence Imaging ◽  
Convolutional Neural Networks ◽  
Frozen Section ◽  
Fluorescent Imaging ◽  
Pathological Diagnosis ◽  
Pathological Examination ◽  
Deep Convolutional Neural Networks ◽  
Fluorescence Images

Abstract Purpose Surgery is the predominant treatment modality of human glioma but suffers difficulty on clearly identifying tumor boundaries in clinic. Conventional practice involves neurosurgeon’s visual evaluation and intraoperative histological examination of dissected tissues using frozen section, which is time-consuming and complex. The aim of this study was to develop fluorescent imaging coupled with artificial intelligence technique to quickly and accurately determine glioma in real-time during surgery. Methods Glioma patients (N = 23) were enrolled and injected with indocyanine green for fluorescence image–guided surgery. Tissue samples (N = 1874) were harvested from surgery of these patients, and the second near-infrared window (NIR-II, 1000–1700 nm) fluorescence images were obtained. Deep convolutional neural networks (CNNs) combined with NIR-II fluorescence imaging (named as FL-CNN) were explored to automatically provide pathological diagnosis of glioma in situ in real-time during patient surgery. The pathological examination results were used as the gold standard. Results The developed FL-CNN achieved the area under the curve (AUC) of 0.945. Comparing to neurosurgeons’ judgment, with the same level of specificity >80%, FL-CNN achieved a much higher sensitivity (93.8% versus 82.0%, P < 0.001) with zero time overhead. Further experiments demonstrated that FL-CNN corrected >70% of the errors made by neurosurgeons. FL-CNN was also able to rapidly predict grade and Ki-67 level (AUC 0.810 and 0.625) of tumor specimens intraoperatively. Conclusion Our study demonstrates that deep CNNs are better at capturing important information from fluorescence images than surgeons’ evaluation during patient surgery. FL-CNN is highly promising to provide pathological diagnosis intraoperatively and assist neurosurgeons to obtain maximum resection safely. Trial registration ChiCTR ChiCTR2000029402. Registered 29 January 2020, retrospectively registered

Radioisotopic Lymphatic Mapping of the Sentinel Node in Melanoma: Importance of Immunohistochemistry

2000 ◽  
Vol 86 (4) ◽  
pp. 346-348 ◽  
Author(s):  
Luca Vaggelli ◽  
Antonio Castagnoli ◽  
Lorenzo Borgognoni ◽  
Carmelo Urso ◽  
Maria Matteini ◽  
...  
Keyword(s):  
Lymph Node ◽  
Sentinel Node ◽  
Primary Tumor ◽  
Frozen Section ◽  
Gamma Probe ◽  
Lymph Node Biopsy ◽  
Pathological Examination ◽  
Blue Dye ◽  
Node Dissection ◽  
Hematoxylin Eosin

Background Elective lymph node dissection (ELND) for patients with malignant melanoma is still controversial. A possible alternative could be biopsy of the first tumor draining lymph node, the sentinel node (SN), which can be identified by means of radionuclide techniques. Aim Our study was undertaken to assess the accuracy of lymph node biopsy and to stress the importance of immunohistochemistry (IHC) in the pathological assessment of the SN for improved staging of the primary tumor. Methods We performed lymphoscintigraphy (LS) in 183 melanoma patients (89 with melanoma of the legs, 11 of the arms and 83 of the trunk). Our protocol consisted of preoperative peritumoral i.d. injection of 99mTc-labeled microcolloid to define the regional lymphatic basin and identify the sentinel node by means of planar scintigraphy. In 147 of the 183 cases a gamma probe (GP) was used during surgery to trace the SN. Vital blue dye was used during surgery in all cases. The SNs were excised for pathological examination. The pathological status of the SN was defined by means of examination of frozen sections, hematoxylin-eosin staining and immunohistochemistry for S-100 and HMB-45 MAb. Results At least one separate focus of activity was identified by LS in 182 out of 183 patients; in all 147 cases where a GP was used, it was successful in tracing the SN. LS with cutaneous mapping of the SN successfully guided the surgical excision in 177 of the 183 cases; in the 7 remaining cases, i.e. 7 out of 83 cases with SNs in the axillary basin, GP was not used and no elective node dissection was performed. Metastases were found in 39 of these 177 cases. In all 39 cases the SNs were the only positive nodes in the basin. Of the 39 metastases 18 were identified by means of frozen section, 12 by means of hematoxylin-eosin, and 9 by means of immunohistochemistry. We therefore emphasize the importance of immunohistochemistry in the pathology of LS for improved staging of the primary tumor.

Brain biopsy sampling by using prospective stereotaxis and a trajectory guide

2001 ◽  
Vol 94 (1) ◽  
pp. 67-71 ◽  
Author(s):  
Walter A. Hall ◽  
Haiying Liu ◽  
Alastair J. Martin ◽  
Robert E. MAxwell ◽  
Charles L. Truwit
Keyword(s):  
Frozen Section ◽  
Spin Echo ◽  
Brain Biopsy ◽  
Pathological Examination ◽  
Single Shot ◽  
Biopsy Needle ◽  
Content Type ◽  
Postoperative Myocardial Infarction ◽  
Clinically Significant ◽  
Fine Print

Object. The authors describe their initial results obtained using a skull-mounted trajectory guide for intraoperative magnetic resonance (MR) imaging—guided brain biopsy sampling. The device was used in conjunction with a new methodology known as prospective stereotaxis for surgical trajectory alignment. Methods. Between January 1999 and March 2000, 38 patients underwent 40 brain biopsy procedures in which prospective stereotaxis was performed with the trajectory guide in a short-bore 1.5-tesla MR imager. In most cases, orthogonal T2-weighted half-Fourier acquisition single-shot turbo spin—echo (HASTE) images were used to determine the desired trajectory and align the device. The surgical trajectory was defined as a line connecting three points: the target, pivot, and alignment stem points. In all cases, surgical specimens were submitted for frozen section and pathological examination. Postoperative turbofluid-attenuated inversion-recovery and gradient-echo images were obtained to exclude the presence of hemorrhage. Trajectory determination and alignment was simple and efficient, requiring less than 5 minutes. Confirmatory HASTE images were obtained along the biopsy needle as it was being advanced or after reaching the target. All biopsy procedures yielded diagnostic tissue. One patient with a lesion near the motor strip experienced a transient hemiparesis of the hand related to passage of the biopsy needle, and another sustained a fatal postoperative myocardial infarction. No patient suffered a clinically significant or radiologically visible hemorrhage. Conclusions. In combination with prospective stereotaxis, the trajectory guide provided a safe and accurate way to perform brain biopsy procedures.

scholarly journals Granulomatous Mastitis: Unusual Presentation and Management, Experience at Birdem General Hospital

2018 ◽  
Vol 30 (2) ◽  
pp. 78-80
Author(s):  
Tamanna Narmeen ◽  
MM Masud Pervez
Keyword(s):  
General Hospital ◽  
Extrapulmonary Tuberculosis ◽  
Granulomatous Inflammation ◽  
Giant Cells ◽  
Abscess Formation ◽  
Reproductive Age ◽  
Unusual Presentation ◽  
Pathological Examination ◽  
Study Group ◽  
Age Group

Tuberculosis of breast is an unusual presentation of extrapulmonary tuberculosis. It occurs mostly in women who are of reproductive age group, multiparous and lactating. Common presentation is tumor like but patients of our study group mostly presented with abscess and mastitis. This study was carried out at BIRDEM GENERAL HOSPITAL, ShegunBagicha, Dhaka. Retrospective data of 9 patients with breast tuberculosis was analyzed. Information about demographic details, clinical presentation, cytology, histopathology, comorbidity and management was taken into account. Out of 1202 patients who presented with breast problem during the study period only 9 (0.75%) suffered from tuberculosis of breast. Their age ranged from 20 to 55 years. All patients except 2 presented with abscess formation, 1 with lump and 1 with multiple sinuses. All patients were subjected to histopathology. Their pathological examination showed chronic granulomatous inflammation with caseous necrosis and langerhans giant cells suggestive of tuberculosis. All 9 patients were treated with Anti TB drug therapy after adequate surgical intervention when required. The rare entity tubercular mastitis is a disease of relatively younger age group. Though the usual presentation is tumor like, the patients of the study group mainly presented with abscess formation. Diabetes might be a contributing factor for the unusual presentation among the patients of my study group.Medicine Today 2018 Vol.30(2): 78-80

scholarly journals Characteristic of Patients with Gastric Perforation due to Peptic Ulcer in Dr. Soetomo General Hospital Surabaya in the Period of January - December 2016

2019 ◽  
Vol 3 (2) ◽  
pp. 45
Author(s):  
Esthiningrum Dewi Agustin ◽  
Mamiek Dwi Putro ◽  
Herry Purbayu
Keyword(s):  
Peptic Ulcer ◽  
Retrospective Study ◽  
Traditional Medicine ◽  
General Hospital ◽  
Medical Record ◽  
Medical Records ◽  
Gastric Perforation ◽  
Pathological Examination ◽  
Lack Of Information

Aim: This study aims to study the profile of patients with gastric perforation caused by peptic ulcer. Method: This is a retrospective study by obtaining patients data from medical records in the Medical Record Centre of Dr. Soetomo General Hospital during the period of January - December 2016 and analyzed descriptively. Result: 66 patients were identified (45 male and 21 female), most of them were between age 60 - 69 years old, and live in Surabaya (45.45%). 30.3% of patients No malignancy was found during the anatomic-pathological examination. 30.3% of patients have a long term NSAID taking, while 53.03% of patients came with a habit of traditional medicine consumption. Perforations were mostly located 1 - 5 cm pre-pyloric and between the range 0.5 - 1 cm in diameter (56.6% and 69.7%, respectively). Conclusion: With the lack of information, it still needs further studies with more completed data for better accuracy.

scholarly journals Papillo-Choledochectomy in the Operative Management of Mucosal Neoplasms of the Periampullary Region

HPB Surgery ◽  
1993 ◽  
Vol 6 (3) ◽  
pp. 211-217
Author(s):  
Kogoro Kasahara ◽  
Ken Saito ◽  
Yasuo Kondo ◽  
Toshihiko Yasuda ◽  
Yoshikazu Yasuda ◽  
...  
Keyword(s):  
Bile Duct ◽  
Frozen Section ◽  
Sphincter Of Oddi ◽  
Operative Management ◽  
Pathological Examination ◽  
Papilla Of Vater ◽  
Radical Cure ◽  
Mucosal Layer ◽  
Periampullary Region ◽  
Longitudinal Muscles

Two patients with mucosal cancer of the periampullary region were treated with papillocholedochectomy, which entails removal of the papilla of Vater and the whole length of the common bile duct. The neoplasm is dissected out through the plane between the duodenal circular and longitudinal muscles, deep to the sphincter of Oddi and the fibromuscular layer of the bile duct. Pathological examination showed that cancer was confined to the mucosal layer without stromal invasion, and that the operation achieved radical cure. For mucosal cancer, papillo-choledochectomy is an alternative to pancreatoduodenectomy, provided that repeated frozen-section studies confirm the completeness of excision.

Sign in / Sign up

Export Citation Format

Share Document