scholarly journals A Tunable 3D Printed Microfluidic Resistive Pulse Sensor for the Characterisation of Algae and Microplastics

Author(s):  
Mark Platt ◽  
Eugenie Hunsicker ◽  
Marcus Pollard

Technologies that can detect and characterise particulates in liquids have applications in health, food and environmental monitoring. Here we present a low-cost and high-throughput multiuse counter that classifies a particle’s size, concentration, porosity and shape. Using an additive manufacturing process, we have assembled a reusable flow resistive pulse sensor. The device remains stable for several days with repeat measurements. We demonstrate its use for characterising algae with spherical and rod structures as well as microplastics shed from teabags. We present a methodology that results in a specific signal for microplastics, namely a conductive pulse, in contrast to particles with smooth surfaces such as calibration particles or algae, allowing the presence of microplastics to be easily confirmed and quantified. In addition, the shape of the signal and particle are correlated, giving an extra physical property to characterise suspended particulates. The technology can rapidly screen volumes of liquid, 1 mL/ min, for the presence of microplastics and algae.<br>

Author(s):  
Mark Platt ◽  
Eugenie Hunsicker ◽  
Marcus Pollard

Technologies that can detect and characterise particulates in liquids have applications in health, food and environmental monitoring. Here we present a low-cost and high-throughput multiuse counter that classifies a particle’s size, concentration, porosity and shape. Using an additive manufacturing process, we have assembled a reusable flow resistive pulse sensor. The device remains stable for several days with repeat measurements. We demonstrate its use for characterising algae with spherical and rod structures as well as microplastics shed from teabags. We present a methodology that results in a specific signal for microplastics, namely a conductive pulse, in contrast to particles with smooth surfaces such as calibration particles or algae, allowing the presence of microplastics to be easily confirmed and quantified. In addition, the shape of the signal and particle are correlated, giving an extra physical property to characterise suspended particulates. The technology can rapidly screen volumes of liquid, 1 mL/ min, for the presence of microplastics and algae.<br>


Coatings ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 422
Author(s):  
Dana Ashkenazi ◽  
Alexandra Inberg ◽  
Yosi Shacham-Diamand ◽  
Adin Stern

Additive manufacturing (AM) revolutionary technologies open new opportunities and challenges. They allow low-cost manufacturing of parts with complex geometries and short time-to-market of products that can be exclusively customized. Additive manufactured parts often need post-printing surface modification. This study aims to review novel environmental-friendly surface finishing process of 3D-printed AlSi10Mg parts by electroless deposition of gold, silver, and gold–silver alloy (e.g., electrum) and to propose a full process methodology suitable for effective metallization. This deposition technique is simple and low cost method, allowing the metallization of both conductive and insulating materials. The AlSi10Mg parts were produced by the additive manufacturing laser powder bed fusion (AM-LPBF) process. Gold, silver, and their alloys were chosen as coatings due to their esthetic appearance, good corrosion resistance, and excellent electrical and thermal conductivity. The metals were deposited on 3D-printed disk-shaped specimens at 80 and 90 °C using a dedicated surface activation method where special functionalization of the printed AlSi10Mg was performed to assure a uniform catalytic surface yielding a good adhesion of the deposited metal to the substrate. Various methods were used to examine the coating quality, including light microscopy, optical profilometry, XRD, X-ray fluorescence, SEM–energy-dispersive spectroscopy (EDS), focused ion beam (FIB)-SEM, and XPS analyses. The results indicate that the developed coatings yield satisfactory quality, and the suggested surface finishing process can be used for many AM products and applications.


Author(s):  
Frank Celentano ◽  
Nicholas May ◽  
Edward Simoneau ◽  
Richard DiPasquale ◽  
Zahra Shahbazi ◽  
...  

Professional musicians today often invest in obtaining antique or vintage instruments. These pieces can be used as collector items or more practically, as performance instruments to give a unique sound of a past music era. Unfortunately, these relics are rare, fragile, and particularly expensive to obtain for a modern day musician. The opportunity to reproduce the sound of an antique instrument through the use of additive manufacturing (3D printing) can make this desired product significantly more affordable. 3D printing allows for duplication of unique parts in a low cost and environmentally friendly method, due to its minimal material waste. Additionally, it allows complex geometries to be created without the limitations of other manufacturing techniques. This study focuses on the primary differences, particularly sound quality and comfort, between saxophone mouthpieces that have been 3D printed and those produced by more traditional methods. Saxophone mouthpieces are commonly derived from a milled blank of either hard rubber, ebonite or brass. Although 3D printers can produce a design with the same or similar materials, they are typically created in a layered pattern. This can potentially affect the porosity and surface of a mouthpiece, ultimately affecting player comfort and sound quality. To evaluate this, acoustic tests will be performed. This will involve both traditionally manufactured mouthpieces and 3D prints of the same geometry created from x-ray scans obtained using a ZEISS Xradia Versa 510. The scans are two dimensional images which go through processes of reconstruction and segmentation, which is the process of assigning material to voxels. The result is a point cloud model, which can be used for 3D printing. High quality audio recordings of each mouthpiece will be obtained and a sound analysis will be performed. The focus of this analysis is to determine what qualities of the sound are changed by the manufacturing method and how true the sound of a 3D printed mouthpiece is to its milled counterpart. Additive manufacturing can lead to more inconsistent products of the original design due to the accuracy, repeatability and resolution of the printer, as well as the layer thickness. In order for additive manufacturing to be a common practice of mouthpiece manufacturing, the printer quality must be tested for its precision to an original model. The quality of a 3D print can also have effects on the comfort of the player. Lower quality 3D prints have an inherent roughness which can cause discomfort and difficulty for the musician. This research will determine the effects of manufacturing method on the sound quality and overall comfort of a mouthpiece. In addition, we will evaluate the validity of additive manufacturing as a method of producing mouthpieces.


BioTechniques ◽  
2021 ◽  
Author(s):  
Vedika J Shenoy ◽  
Chelsea ER Edwards ◽  
Matthew E Helgeson ◽  
Megan T Valentine

3D printing holds potential as a faster, cheaper alternative compared with traditional photolithography for the fabrication of microfluidic devices by replica molding. However, the influence of printing resolution and quality on device design and performance has yet to receive detailed study. Here, we investigate the use of 3D-printed molds to create staggered herringbone mixers (SHMs) with feature sizes ranging from ∼100 to 500 μm. We provide guidelines for printer calibration to ensure accurate printing at these length scales and quantify the impacts of print variability on SHM performance. We show that SHMs produced by 3D printing generate well-mixed output streams across devices with variable heights and defects, demonstrating that 3D printing is suitable and advantageous for low-cost, high-throughput SHM manufacturing.


Author(s):  
Ashish Jagtiani ◽  
Jiang Zhe ◽  
Bi-min Zhang Newby

We describe an all-electronic, label-free, resistive pulse sensor that utilizes multiple micropores for simultaneous detection and counting of multiple biological particles. Four particle samples were utilized for the sensor testing: 20μm and 40μm polymethacrylate (PM) particles, Juniper pollen and Eastern Cottonwood pollen. Experiments demonstrated that this sensor was able to differentiate and count multiple particle solutions simultaneously through its four micropores fabricated on polymer membranes. Thus the sensing throughput has been improved significantly. Furthermore, the experimental results also proved the feasibility of differentiating various pollens from PM microparticles with the multi-pore resistive pulse sensor, with no need for labeling of samples.


2019 ◽  
Author(s):  
Noha Hamada Mohamed ◽  
Hossam Kandil ◽  
Iman Ismail Dakhli

Abstract In dentistry, 3D printing already has diverse applicability, and holds a great deal of promise to make possible many new and exciting treatments and approaches to manufacturing dental restorations. Better availability, shorter processing time, and descending costs have resulted in the increased use of RP. Concomitantly the development of medical applications is expanding. (Zaharia et al., 2017)Many different printing technologies exist, each with their own advantages and disadvantages. Unfortunately, a common feature of the more functional and productive equipment is the high cost of the equipment, the materials, maintenance, and repair, often accompanied by a need for messy cleaning, difficult post-processing, and sometimes onerous health and safety concerns (Dawood et al., 2015)Low-cost 3D printers represent a great opportunity in the dental and medical field, as they could allow surgeons to use 3D models at a very low cost and, therefore, democratize the use of these 3D models in various indications. However, efforts should be made to establish a unified validation protocol for low-cost RP 3D printed models, including accuracy, reproducibility, and repeatability tests. Asaumi et al., suggested that dimensional changes may not affect the success of surgical applications if such changes are within a 2% variation .However, the proposed cut-off of 2% should be furthermore discussed, as the same accuracy may be not required for all types of indications. (Silva et al., 2008; Maschio et al., 2016)This aim of the present study is to evaluate the dimensional accuracy of the 3D printed mandibular models fabricated by two different additive manufacturing techniques, using highly precise one as selective laser sintering (SLS) and a low-cost one as fused filament fabrication and whether they are both comparable in terms of precision. In addition to evaluation of dimensional accuracy of linear measurements of the mandible in CBCT scans.7 mandibular models will be recruited. Radio-opaque markers of gutta-percha balls will be applied on the model to act as guide pointsTen linear measurements (5 long distances: Inter-condylar, inter-coronoidal, inter-mandibular notch, length of left ramus, length of right ramus; as well as 5 short distances: Length of the body of the mandible at midline, length of the body of the mandible in the area of last left molar, as well as that of the last right molar, the distance between the tip of right condyle to the tip of the right coronoid, as well as that of their left counterparts) will be obtained using digital calliper, to act as the reference standard later. Scanning of the model by CBCT will be next , 3D printing of the scanned image using SLS and FFF printers will be done. Recording of same linear measurment will be done on printed models. Comparison of the recorded values vs reference standard is the last step


2020 ◽  
Vol 4 (3) ◽  
pp. 94 ◽  
Author(s):  
Arash Afshar ◽  
Roy Wood

Additive manufacturing, or 3D printing, has had a big impact on the manufacturing world through its low cost, material recyclability, and fabrication of intricate geometries with a high resolution. Three-dimensionally printed polymer structures in aerospace, marine, construction, and automotive industries are usually intended for service in outdoor environments. During long-term exposures to harsh environmental conditions, the mechanical properties of these structures can be degraded significantly. Developing coating systems for 3D printed parts that protect the structural surface against environmental effects and provide desired surface properties is crucial for the long-term integrity of these structures. In this study, a novel method was presented to create 3D printed structures coated with a weather-resistant material in a single manufacturing operation using multi-material additive manufacturing. One group of specimens was 3D printed from acrylonitrile-butadiene-styrene (ABS) material and the other group was printed from ABS and acrylic-styrene-acrylonitrile (ASA) as a substrate and coating material, respectively. The uncoated ABS specimens suffered significant degradation in the mechanical properties, particularly in the failure strain and toughness, during exposure to UV radiation, moisture, and high temperature. However, the ASA coating preserved the mechanical properties and structural integrity of ABS 3D printed structures in aggressive environments.


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