scholarly journals CNC-Milled Superhydrophobic Macroporous Monoliths for 3D Cell Culture

2020 ◽  
Author(s):  
Gen Hayase ◽  
Daisuke Yoshino
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
High Strength ◽  
Cell Types ◽  
Computer Numerical Control ◽  
Numerical Control ◽  
Cnc Milling ◽  
Water Droplets ◽  
Machined Surface

High-strength macroporous monoliths can be obtained by simply mixing boehmite nanofiber aqueous acetate dispersions with methyltrimethoxysilane. On the boehmite nanofiber-polymethylsilsesquioxane monoliths, we can fabricate structures smaller than a millimeter in size by computer numerical control (CNC) milling, resulting in a machined surface that is superhydrophobic and biocompatible. Using this strategy, we fabricated a superhydrophobic multiwell plate which holds water droplets to produce 3D cell culture environments for various cell types. We expect these superhydrophobic monoliths to have future applications in 3D tissue construction.

scholarly journals CNC-Milled Superhydrophobic Macroporous Monoliths for 3D Cell Culture

2020 ◽  
Author(s):  
Gen Hayase ◽  
Daisuke Yoshino
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
High Strength ◽  
Cell Types ◽  
Computer Numerical Control ◽  
Numerical Control ◽  
Cnc Milling ◽  
Water Droplets ◽  
Machined Surface

High-strength macroporous monoliths can be obtained by simply mixing boehmite nanofiber aqueous acetate dispersions with methyltrimethoxysilane. On the boehmite nanofiber-polymethylsilsesquioxane monoliths, we can fabricate structures smaller than a millimeter in size by computer numerical control (CNC) milling, resulting in a machined surface that is superhydrophobic and biocompatible. Using this strategy, we fabricated a superhydrophobic multiwell plate which holds water droplets to produce 3D cell culture environments for various cell types. We expect these superhydrophobic monoliths to have future applications in 3D tissue construction.

CNC-Milled Superhydrophobic Macroporous Monoliths for 3D Cell Culture

2020 ◽  
Author(s):  
Gen Hayase ◽  
Daisuke Yoshino
Keyword(s):  
Cell Culture ◽  
3D Cell Culture ◽  
High Strength ◽  
Cell Types ◽  
Computer Numerical Control ◽  
Numerical Control ◽  
Cnc Milling ◽  
Water Droplets ◽  
Machined Surface

High-strength macroporous monoliths can be obtained by simply mixing boehmite nanofiber aqueous acetate dispersions with methyltrimethoxysilane. On the boehmite nanofiber-polymethylsilsesquioxane monoliths, we can fabricate structures smaller than a millimeter in size by computer numerical control (CNC) milling, resulting in a machined surface that is superhydrophobic and biocompatible. Using this strategy, we fabricated a superhydrophobic multiwell plate which holds water droplets to produce 3D cell culture environments for various cell types. We expect these superhydrophobic monoliths to have future applications in 3D tissue construction.

scholarly journals A Low-Cost Vision-Based Monitoring of Computer Numerical Control (CNC) Machine Tools for Small and Medium-Sized Enterprises (SMEs)

Sensors ◽  
2019 ◽  
Vol 19 (20) ◽  
pp. 4506 ◽  
Author(s):  
Hyungjung Kim ◽  
Woo-Kyun Jung ◽  
In-Gyu Choi ◽  
Sung-Hoon Ahn
Keyword(s):  
Open Source ◽  
Machine Tools ◽  
Low Cost ◽  
Computer Numerical Control ◽  
Numerical Control ◽  
Smart Factory ◽  
Cnc Machine Tools ◽  
Shop Floor ◽  
Cnc Milling ◽  
Cnc Machine

In the new era of manufacturing with the Fourth Industrial Revolution, the smart factory is getting much attention as a solution for the factory of the future. Despite challenges in small and medium-sized enterprises (SMEs), such as short-term strategies and labor-intensive with limited resources, they have to improve productivity and stay competitive by adopting smart factory technologies. This study presents a novel monitoring approach for SMEs, KEM (keep an eye on your machine), and using a low-cost vision, such as a webcam and open-source technologies. Mainly, this idea focuses on collecting and processing operational data using cheaper and easy-to-use components. A prototype was tested with the typical 3-axis computer numerical control (CNC) milling machine. From the evaluation, availability of using a low-cost webcam and open-source technologies for monitoring of machine tools was confirmed. The results revealed that the proposed system is easy to integrate and can be conveniently applied to legacy machine tools on the shop floor without a significant change of equipment and cost barrier, which is less than $500 USD. These benefits could lead to a change of monitoring operations to reduce time in operation, energy consumption, and environmental impact for the sustainable production of SMEs.

scholarly journals Aspiration-mediated hydrogel micropatterning using rail-based open microfluidic devices for high-throughput 3D cell culture

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Dohyun Park ◽  
Jungseub Lee ◽  
Younggyun Lee ◽  
Kyungmin Son ◽  
Jin Woo Choi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Capillary Pressure ◽  
Microfluidic Device ◽  
High Throughput ◽  
3D Cell Culture ◽  
Microfluidic Devices ◽  
Cell Types ◽  
Human Organ ◽  
Patterning Technique ◽  
Experimental Yield

AbstractMicrofluidics offers promising methods for aligning cells in physiologically relevant configurations to recapitulate human organ functionality. Specifically, microstructures within microfluidic devices facilitate 3D cell culture by guiding hydrogel precursors containing cells. Conventional approaches utilize capillary forces of hydrogel precursors to guide fluid flow into desired areas of high wettability. These methods, however, require complicated fabrication processes and subtle loading protocols, thus limiting device throughput and experimental yield. Here, we present a swift and robust hydrogel patterning technique for 3D cell culture, where preloaded hydrogel solution in a microfluidic device is aspirated while only leaving a portion of the solution in desired channels. The device is designed such that differing critical capillary pressure conditions are established over the interfaces of the loaded hydrogel solution, which leads to controlled removal of the solution during aspiration. A proposed theoretical model of capillary pressure conditions provides physical insights to inform generalized design rules for device structures. We demonstrate formation of multiple, discontinuous hollow channels with a single aspiration. Then we test vasculogenic capacity of various cell types using a microfluidic device obtained by our technique to illustrate its capabilities as a viable micro-manufacturing scheme for high-throughput cellular co-culture.

scholarly journals Accuracy of Implant Surgical Guides Fabricated Using Computer Numerical Control Milling for Edentulous Jaws: A Pilot Clinical Trial

2020 ◽  
Author(s):  
Jinyou Chai ◽  
Xiaoqian Liu ◽  
Ramona Schweyen ◽  
Jürgen Setz ◽  
Shaoxia Pan ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Computer Numerical Control ◽  
Numerical Control ◽  
Cnc Milling ◽  
Implant Position ◽  
Surgical Guides ◽  
Computer Aided ◽  
Cad Cam ◽  
The Mean ◽  
Edentulous Jaws

Abstract Background To evaluate the accuracy of a computer-aided design and computer-aided manufacturing (CAD-CAM) surgical guide for implant placement in edentulous jaws. Methods Nine patients with twelve edentulous jaws seeking implants were recruited. Radiographic guides with diagnostic templates were fabricated from try-in waxup dentures. Planning software (Organical® Dental Implant, Berlin, Germany) was used to virtually design the implant positions, and the radiographic templates were converted into surgical guides using computer numerical control (CNC) milling. Following the guided implant surgery protocol, forty-four implants were placed into twelve edentulous jaws. Cone-beam computed tomography (CBCT) scans were performed post-operatively for each jaw, and the deviations between the planned and actual implant positions were measured. Results All 44 implants survived, and no severe haematomas, nerve injuries or unexpected sinus perforations occurred. The mean three dimensional linear deviation of implant position between virtual planning and actual placement was 1.53 ± 0.48 mm at the implant neck and 1.58 ± 0.4 mm at the apex. The angular deviation was 3.96 ± 3.05 degrees. The mean deviation between virtual and actual implant position was significantly smaller in the maxilla than in the mandible. No significant differences were found in the deviation of implant position between cases with and without anchor pins. Conclusions The guides fabricated using the CAD-CAM CNC milling technique provided comparable accuracy as those fabricated by Stereolithography. The displacement of the guides on edentulous arch might be the main contributing factor of deviation. Trial registration: Chinese Clinical Trial Registry, ChiCTR-ONC-17014159

scholarly journals Advanced 3D Cell Culture Techniques in Micro-Bioreactors, Part I: A Systematic Analysis of the Literature Published between 2000 and 2020

Processes ◽  
2020 ◽  
Vol 8 (12) ◽  
pp. 1656
Author(s):  
Christoph Grün ◽  
Brigitte Altmann ◽  
Eric Gottwald
Keyword(s):  
Cell Culture ◽  
Large Scale ◽  
3D Cell Culture ◽  
Cell Types ◽  
Cell Contact ◽  
Systematic Analysis ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
3D Microenvironment

Bioreactors have proven useful for a vast amount of applications. Besides classical large-scale bioreactors and fermenters for prokaryotic and eukaryotic organisms, micro-bioreactors, as specialized bioreactor systems, have become an invaluable tool for mammalian 3D cell cultures. In this systematic review we analyze the literature in the field of eukaryotic 3D cell culture in micro-bioreactors within the last 20 years. For this, we define complexity levels with regard to the cellular 3D microenvironment concerning cell–matrix-contact, cell–cell-contact and the number of different cell types present at the same time. Moreover, we examine the data with regard to the micro-bioreactor design including mode of cell stimulation/nutrient supply and materials used for the micro-bioreactors, the corresponding 3D cell culture techniques and the related cellular microenvironment, the cell types and in vitro models used. As a data source we used the National Library of Medicine and analyzed the studies published from 2000 to 2020.

A Novel Suspended Hydrogel Membrane Platform for Cell Culture

2015 ◽  
Vol 6 (2) ◽  
Author(s):  
Yong X. Chen ◽  
Shihao Yang ◽  
Jiahan Yan ◽  
Ming-Han Hsieh ◽  
Lingyan Weng ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Biology ◽  
3D Cell Culture ◽  
Cell Encapsulation ◽  
Cell Types ◽  
Ease Of Use ◽  
In Vitro Assays ◽  
Hydrogel Membrane

Current cell-culture is largely performed on synthetic two-dimensional (2D) petri dishes or permeable supports such as Boyden chambers, mostly because of their ease of use and established protocols. It is generally accepted that modern cell biology research requires new physiologically relevant three-dimensional (3D) cell culture platform to mimic in vivo cell responses. To that end, we report the design and development of a suspended hydrogel membrane (ShyM) platform using gelatin methacrylate (GelMA) hydrogel. ShyM thickness (0.25–1 mm) and mechanical properties (10–70 kPa) can be varied by controlling the size of the supporting grid and concentration of GelMA prepolymer, respectively. GelMA ShyMs, with dual media exposure, were found to be compatible with both the cell-seeding and the cell-encapsulation approach as tested using murine 10T1/2 cells and demonstrated higher cellular spreading and proliferation as compared to flat GelMA unsuspended control. The utility of ShyM was also demonstrated using a case-study of invasion of cancer cells. ShyMs, similar to Boyden chambers, are compatible with standard well-plates designs and can be printed using commonly available 3D printers. In the future, ShyM can be potentially extended to variety of photosensitive hydrogels and cell types, to develop new in vitro assays to investigate complex cell–cell and cell–extracellular matrix (ECM) interactions.

scholarly journals RANCANG BANGUN MESIN CNC MILLING MENGGUNAKAN SYSTEM KONTROL GRBL UNTUK PEMBUATAN LAYOUT PCB

2020 ◽  
Vol 4 (1) ◽  
pp. 37
Author(s):  
Muhammad Jufrizaldy ◽  
Ilyas Ilyas ◽  
Marzuki Marzuki
Keyword(s):  
Power Supply ◽  
Computer Numerical Control ◽  
Numerical Control ◽  
Stepper Motor ◽  
Cnc Milling ◽  
G Code

Mesin CNC milling adalah mesin potong berbasis komputer yang dapat menjalankan proses secara otomatis pada berbagai macam perintah berbasis bahan yang telah diprogram ke dalam perangkat lunak. Pada tugas akhir kali ini akan dirancang dan diimplementasikan CNC (Computer Numerical Control) Router dengan menggunakan Program G-Code dengan menggunakan GBRL Kontroller sebagai pengontrol mesin CNC. Mikrokontroler digunakan untuk menerima G-Code dari PC yang dikirim ke mikrokontroler yang selanjutnya dikontrol menggunakan GBRL kontroller untuk menggerakan motor Stepper. Mesin CNC ini dikendalikan dengan menggunakan software GRBL dimana ketika program dimasukkan kedalam software tersebut, stepper motor, spindle serta mata bor akan bergerak. Perancangan ini menggunakan 3 buah stepper motor dimana setiap stepper motor berfungsi untuk menggerakkan sumbu X, Y dan Z. Spindle digunakan sebagai pengendali mata bor yang berfungsi untuk mengukir layout pada PCB.Kata kunci : Motor Stepper NEMA , Driver Motor Board T, Breakout Board , Power supply , Spindle Kit

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