Heterogeneous Adsorption and Local Ordering of Formate on a Magnetite Surface

We report a novel heterogeneous adsorption mechanism of formic acid on the magnetite (111) surface. Our experimental results and DFT calculations give evidence for dissociative adsorption of formic acid in quasi-bidentate and chelating geometries. The latter is induced by the presence of iron vacancies at the surface, making oxygen atoms accessible for hydrogen atoms from dissociated formic acid. DFT calculations predict that both adsorption geometries are energetically favorable under our experimental conditions. The calculations prove that the locally observed (sqrt(3)xsqrt(3)) R30° superstructure consists of formate in a triangular arrangement, adsorbed predominantly in chelating geometry. The results show how defects can stabilize alternative adsorption geometries, which is a crucial ingredient for a detailed atomistic understanding of reaction barriers on magnetite and other oxide surfaces, as well as for the stability of carboxylic acid based nanocomposite materials.

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Applicability of Instability Index for In vitro Protein Stability Prediction

Protein and Peptide Letters ◽

10.2174/0929866526666190228144219 ◽

2019 ◽

Vol 26 (5) ◽

pp. 339-347 ◽

Cited By ~ 4

Author(s):

Dilani G. Gamage ◽

Ajith Gunaratne ◽

Gopal R. Periyannan ◽

Timothy G. Russell

Keyword(s):

Protein Stability ◽

Caulobacter Crescentus ◽

Effect Of Temperature ◽

Instability Index ◽

Dipeptide Composition ◽

Experimental Conditions ◽

The Stability ◽

Vitro Protein

Background: The dipeptide composition-based Instability Index (II) is one of the protein primary structure-dependent methods available for in vivo protein stability predictions. As per this method, proteins with II value below 40 are stable proteins. Intracellular protein stability principles guided the original development of the II method. However, the use of the II method for in vitro protein stability predictions raises questions about the validity of applying the II method under experimental conditions that are different from the in vivo setting. Objective: The aim of this study is to experimentally test the validity of the use of II as an in vitro protein stability predictor. Methods: A representative protein CCM (CCM - Caulobacter crescentus metalloprotein) that rapidly degrades under in vitro conditions was used to probe the dipeptide sequence-dependent degradation properties of CCM by generating CCM mutants to represent stable and unstable II values. A comparative degradation analysis was carried out under in vitro conditions using wildtype CCM, CCM mutants and two other candidate proteins: metallo-β-lactamase L1 and α -S1- casein representing stable, borderline stable/unstable, and unstable proteins as per the II predictions. The effect of temperature and a protein stabilizing agent on CCM degradation was also tested. Results: Data support the dipeptide composition-dependent protein stability/instability in wt-CCM and mutants as predicted by the II method under in vitro conditions. However, the II failed to accurately represent the stability of other tested proteins. Data indicate the influence of protein environmental factors on the autoproteolysis of proteins. Conclusion: Broader application of the II method for the prediction of protein stability under in vitro conditions is questionable as the stability of the protein may be dependent not only on the intrinsic nature of the protein but also on the conditions of the protein milieu.

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Macropolyhedral Boron-Containing Cluster Chemistry. A Metallathiaborane from S2B17H17: Isolation and Characterisation of [(PMe2Ph)2PtS2B16H16]; A neo-arachno Ten-Vertex Cluster Shape, and the Constitution of the [arachno-B10H15]- Anion

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc20050430 ◽

2005 ◽

Vol 70 (4) ◽

pp. 430-440 ◽

Cited By ~ 9

Author(s):

Michael J. Carr ◽

Michael G. S. Londesborough ◽

Jonathan Bould ◽

Ivana Císařová ◽

Bohumil Štíbr ◽

...

Keyword(s):

Dft Calculations ◽

Sodium Hydride ◽

Hydrogen Atoms ◽

Subsequent Reaction ◽

Cluster Chemistry ◽

Cluster Shape ◽

Open Face

The deprotonation of S2B17H17 with sodium hydride and subsequent reaction with [PtCl2(PMe2Ph)2] gives the new macropolyhedral metallathiaborane [(PMe2Ph)2PtS2B16H16], of which the cluster consists of a conventional eleven-vertex nido {SB10} unit, fused, with two boron atoms in common, with a {PtSB8} unit of unique ten-vertex neo-arachno constitution and geometry. The latter geometry suggests a configuration for the previously structurally uncharacterised [B10H15]- anion; starting from this configuration, DFT calculations of structure and thence of boron nuclear shieldings, which are found very closely to mimic those found experimentally, thence support a fluxional structure for [B10H15]- with three {BHB(bridging)} and two {BH(endo)} hydrogen atoms around a six-membered open face.

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Deciphering the Mechanistic Details of Manganese-Catalyzed Formic Acid Dehydrogenation: Insights from DFT Calculations

Inorganic Chemistry ◽

10.1021/acs.inorgchem.1c00757 ◽

2021 ◽

Author(s):

Neethinathan Johnee Britto ◽

Madhavan Jaccob

Keyword(s):

Dft Calculations ◽

Formic Acid ◽

Formic Acid Dehydrogenation

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Effective Separation of Prime Olefins from Gas Stream Using Anion Pillared Metal Organic Frameworks: Ideal Adsorbed Solution Theory Studies, Cyclic Application and Stability

Catalysts ◽

10.3390/catal11040510 ◽

2021 ◽

Vol 11 (4) ◽

pp. 510

Author(s):

Majeda Khraisheh ◽

Fares. Almomani ◽

Gavin Walker

Keyword(s):

Breakthrough Curves ◽

Isotherm Models ◽

Regeneration Ability ◽

Solution Theory ◽

Experimental Conditions ◽

Ideal Adsorbed Solution Theory ◽

Adsorbed Solution ◽

The Stability ◽

Adsorption Desorption ◽

Adsorbed Solution Theory

The separation of C3H4/C3H6 is one of the most energy intensive and challenging operations, requiring up to 100 theoretical stages, in traditional cryogenic distillation. In this investigation, the potential application of two MOFs (SIFSIX-3-Ni and NbOFFIVE-1-Ni) was tested by studying the adsorption–desorption behaviors at a range of operational temperatures (300–360 K) and pressures (1–100 kPa). Dynamic adsorption breakthrough tests were conducted and the stability and regeneration ability of the MOFs were established after eight consecutive cycles. In order to establish the engineering key parameters, the experimental data were fitted to four isotherm models (Langmuir, Freundlich, Sips and Toth) in addition to the estimation of the thermodynamic properties such as the isosteric heats of adsorption. The selectivity of the separation was tested by applying ideal adsorbed solution theory (IAST). The results revealed that SIFSIX-3-Ni is an effective adsorbent for the separation of 10/90 v/v C3H4/C3H6 under the range of experimental conditions used in this study. The maximum adsorption reported for the same combination was 3.2 mmolg−1. Breakthrough curves confirmed the suitability of this material for the separation with a 10-min gab before the lighter C3H4 is eluted from the column. The separated C3H6 was obtained with a 99.98% purity.

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Relative versus absolute RNA quantification: a comparative analysis based on the example of endothelial expression of vasoactive receptors

Biological Procedures Online ◽

10.1186/s12575-021-00144-w ◽

2021 ◽

Vol 23 (1) ◽

Author(s):

Kevin Kuhlmann ◽

Melanie Cieselski ◽

Julia Schumann

Keyword(s):

Dynamic Range ◽

Genetic Material ◽

Housekeeping Genes ◽

Digital Pcr ◽

Absolute Quantification ◽

Test Procedures ◽

Experimental Conditions ◽

Rna Quantification ◽

The Stability ◽

Inflammatory Milieu

Abstract Background In the present study, two distinct PCR methods were used for the quantification of genetic material and their results were compared: real-time-PCR (qPCR; relative quantification) and droplet digital PCR (ddPCR; absolute quantification). The comparison of the qPCR and the ddPCR was based on a stimulation approach of microvascular endothelial cells in which the effect of a pro-inflammatory milieu on the expression of vasoactive receptors was investigated. Results There was consistency in directions of effects for the majority of genes tested. With regard to the indicated dimension of the effects, the overall picture was more differentiated. It was striking that deviations were more pronounced if the measured values were on the extreme edges of the dynamic range of the test procedures. Conclusions To obtain valid and reliable results, dilution series are recommended, which should be carried out initially. In case of ddPCR the number of copies per µl should be adjusted to the low three-digit range. With regard to qPCR it is essential that the stability and reliability of the reference genes used is guaranteed. Here, ddPCR offers the advantage that housekeeping genes are not required. Furthermore, an absolute quantification of the sample can be easily performed by means of ddPCR. Before using ddPCR, however, care should be taken to optimize the experimental conditions. Strict indications for this methodology should also be made with regard to economic and timing factors.

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Effective replacement of cetyltrimethylammonium bromide (CTAB) by mercaptoalkanoic acids on gold nanorod (AuNR) surfaces in aqueous solutions

Nanoscale ◽

10.1039/c9nr09137h ◽

2020 ◽

Vol 12 (2) ◽

pp. 658-668 ◽

Cited By ~ 6

Author(s):

Rafael del Caño ◽

Jose M. Gisbert-González ◽

Jose González-Rodríguez ◽

Guadalupe Sánchez-Obrero ◽

Rafael Madueño ◽

...

Keyword(s):

Aqueous Solutions ◽

Gold Nanorods ◽

Ligand Exchange ◽

Cetyltrimethylammonium Bromide ◽

Gold Nanorod ◽

Experimental Conditions ◽

Seed Mediated ◽

The Stability

The highly packed cetyltrimethylammonium bromide bilayer on the surface of gold nanorods synthesized by the seed-mediated procedure hampers the complete ligand exchange under experimental conditions that preserves the stability of the dispersions.

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Investigations of ternary complexes of Co(II) and Ni(II) with thiodiacetate anion and 1,10-phenanthroline or 2,2’-bipyridine in aqueous solutions

Open Chemistry ◽

10.1515/chem-2015-0048 ◽

2014 ◽

Vol 13 (1) ◽

Author(s):

Dariusz Wyrzykowski ◽

Joanna Pranczk ◽

Dagmara Jacewicz ◽

Aleksandra Tesmar ◽

Bogusław Pilarski ◽

...

Keyword(s):

Aqueous Solutions ◽

Substitution Reactions ◽

Experimental Conditions ◽

Kinetic Measurements ◽

Hydroxo Complexes ◽

Binary Complexes ◽

Vis Spectroscopy ◽

Potentiometric Titration Method ◽

The Stability ◽

Ph Range

AbstractA potentiometric titration method (PT) and a stopped-flow kinetic technique monitored by a UV−Vis spectroscopy have been used to characterize the stability of series of Co(II)- and Ni(II)-thiodiacetato complexes, M(TDA), in the presence of 1,10-phenanthroline (phen) or 2,2’-bipyridine (bipy) in aqueous solutions. The stability constants of the binary (1:1), ternary (1:1:1) as well as the resulting hydroxo complexes were evaluated and compared to the corresponding oxydiacetate complexes. Based on the species distribution as a function of pH the relative predominance of the species in the system over a pH range was discussed. Furthermore, the kinetic measurements of the substitution reactions of the aqua ligands to phen or bipy in the coordination sphere of the binary complexes M(TDA) were performed in the 288–303 K temperature range, at a constant concentration of phen or bipy and at seven different concentrations of the binary complexes (0.2–0.5 mM). The kinetic stability of the M(TDA) complexes was discussed in relation to the experimental conditions and the kind of the auxiliary ligands (phen/bipy). Moreover, the influence of the type of primary ligand (thiodiacetate/oxydiacetate) on the substitution rate of the auxiliary ligands was also compared.

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Adsorption mechanism of two pesticides on polyethylene and polypropylene microplastics: DFT calculations and particle size effects

Environmental Pollution ◽

10.1016/j.envpol.2021.118120 ◽

2021 ◽

Vol 291 ◽

pp. 118120

Author(s):

Qiming Mo ◽

Xingjian Yang ◽

Jinjin Wang ◽

Huijuan Xu ◽

Wenyan Li ◽

...

Keyword(s):

Particle Size ◽

Dft Calculations ◽

Size Effects ◽

Adsorption Mechanism ◽

Particle Size Effects

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Current Practices for Reference Gene Selection in RT-qPCR of Aspergillus: Outlook and Recommendations for the Future

Genes ◽

10.3390/genes12070960 ◽

2021 ◽

Vol 12 (7) ◽

pp. 960

Author(s):

Meagan Archer ◽

Jianping Xu

Keyword(s):

Gene Expression ◽

Reference Gene ◽

Reference Genes ◽

Gene Selection ◽

Quantitative Polymerase Chain Reaction ◽

Specific Method ◽

Experimental Conditions ◽

Reference Gene Selection ◽

Physiological Processes ◽

The Stability

Aspergillus is a genus of filamentous fungi with vast geographic and ecological distributions. Species within this genus are clinically, agriculturally and biotechnologically relevant, leading to increasing interest in elucidating gene expression dynamics of key metabolic and physiological processes. Reverse-transcription quantitative Polymerase Chain Reaction (RT-qPCR) is a sensitive and specific method of quantifying gene expression. A crucial step for comparing RT-qPCR results between strains and experimental conditions is normalisation to experimentally validated reference gene(s). In this review, we provide a critical analysis of current reference gene selection and validation practices for RT-qPCR gene expression analyses of Aspergillus. Of 90 primary research articles obtained through our PubMed query, 17 experimentally validated the reference gene(s) used. Twenty reference genes were used across the 90 studies, with beta-tubulin being the most used reference gene, followed by actin, 18S rRNA and glyceraldehyde 3-phosphate dehydrogenase. Sixteen of the 90 studies used multiple reference genes for normalisation. Failing to experimentally validate the stability of reference genes can lead to conflicting results, as was the case for four studies. Overall, our review highlights the need to experimentally validate reference genes in RT-qPCR studies of Aspergillus.

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