Microfluidic Quantification of Yeast Surface Adhesion

10.26434/chemrxiv.6446432 ◽

2018 ◽

Author(s):

Kristina Reinmets ◽

Amin Dehkharghani ◽

Jeffrey S. Guasto ◽

Stephen Fuchs

Keyword(s):

Genetic Background ◽

Food Production ◽

Rapid Screening ◽

Surface Adhesion ◽

Adhesive Properties ◽

Fungal Adhesion ◽

Yeast Surface ◽

Population Scale

<div>Fungal adhesion is fundamental to processes ranging from</div><div>infection to food production yet, robust, population-scale</div><div>quantification methods for yeast surface adhesion are lacking. We developed a microfluidic assay to distinguish the effects of genetic background and solution conditions on adhesion. This approach will enable the rapid screening of yeast adhesive properties for anti-fouling surfaces and a host of other applications.</div>

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Microfluidic quantification and separation of yeast based on surface adhesion

Lab on a Chip ◽

10.1039/c9lc00275h ◽

2019 ◽

Vol 19 (20) ◽

pp. 3481-3489 ◽

Cited By ~ 1

Author(s):

Kristina Reinmets ◽

Amin Dehkharghani ◽

Jeffrey S. Guasto ◽

Stephen M. Fuchs

Keyword(s):

Food Production ◽

Rapid Screening ◽

Surface Adhesion ◽

Adhesive Properties ◽

Fungal Adhesion

Fungal adhesion is fundamental to processes ranging from infections to food production. We developed a microfluidic assay for rapid screening and fractionation of genetically-related yeast based on adhesive properties.

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Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in C. albicans

Fermentation ◽

10.3390/fermentation6010028 ◽

2020 ◽

Vol 6 (1) ◽

pp. 28 ◽

Cited By ~ 3

Author(s):

Anne-Céline Kohler ◽

Leonardo Venturelli ◽

Abhilash Kannan ◽

Dominique Sanglard ◽

Giovanni Dietler ◽

...

Keyword(s):

Antifungal Drugs ◽

Mucosal Surface ◽

Public Health Issue ◽

Adhesive Properties ◽

Simple Manner ◽

Major Public Health Issue ◽

Force Microscopy ◽

Fungal Adhesion ◽

Atomic Force ◽

Induced Changes

Yeast resistance to antifungal drugs is a major public health issue. Fungal adhesion onto the host mucosal surface is still a partially unknown phenomenon that is modulated by several actors among which fibronectin plays an important role. Targeting the yeast adhesion onto the mucosal surface could lead to potentially highly efficient treatments. In this work, we explored the effect of fibronectin on the nanomotion pattern of different Candida albicans strains by atomic force microscopy (AFM)-based nanomotion detection and correlated the cellular oscillations to the yeast adhesion onto epithelial cells. Preliminary results demonstrate that strongly adhering strains reduce their nanomotion activity upon fibronectin exposure whereas low adhering Candida remain unaffected. These results open novel avenues to explore cellular reactions upon exposure to stimulating agents and possibly to monitor in a rapid and simple manner adhesive properties of C. albicans.

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Stress Resistance and Adhesive Properties of Commercial Flor and Wine Strains, and Environmental Isolates of Saccharomyces cerevisiae

Fermentation ◽

10.3390/fermentation7030188 ◽

2021 ◽

Vol 7 (3) ◽

pp. 188

Author(s):

Michail A. Eldarov ◽

Daria A. Avdanina ◽

Elena Ivanova ◽

Maksim Y. Shalamitskiy ◽

Tatiana N. Tanashchuk ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Stress Resistance ◽

Rapid Screening ◽

Clear Correlation ◽

Yeast Culture ◽

Environmental Isolates ◽

Adhesive Properties ◽

Experimental Conditions ◽

Culture Collections ◽

Flor Yeast

Flor strains of Saccharomyces cerevisiae represent a special group of yeasts used for producing biologically aged wines. We analyzed the collection of commercial wine and flor yeast strains, as well as environmental strains isolated from the surface of grapes growing in vineyards, for resistance to abiotic stresses, adhesive properties, and the ability to form a floating flor. The degree of resistance of commercial strains to ethanol, acetaldehyde, and hydrogen peroxide was generally not higher than that of environmental isolates, some of which had high resistance to the tested stress agents. The relatively low degree of stress resistance of flor strains can be explained both by the peculiarities of their adaptive mechanisms and by differences in the nature of their exposure to various types of stress in the course of biological wine aging and under the experimental conditions we used. The hydrophobicity and adhesive properties of cells were determined by the efficiency of adsorption to polystyrene and the distribution of cells between the aqueous and organic phases. Flor strains were distinguished by a higher degree of hydrophobicity of the cell surface and an increased ability to adhere to polystyrene. A clear correlation between biofilm formation and adhesive properties was also observed for environmental yeast isolates. The overall results of this study indicate that relatively simple tests for cell hydrophobicity can be used for the rapid screening of new candidate flor strains in yeast culture collections and among environmental isolates.

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On the Modifiection of Polyethyleneterephtplgte Surfaces to Improve Pohesion

MRS Proceedings ◽

10.1557/proc-119-235 ◽

1988 ◽

Vol 119 ◽

Cited By ~ 1

Author(s):

E. Occhiello ◽

F. Gprbassi ◽

G. Morini

Keyword(s):

Surface Chemistry ◽

Alkaline Solutions ◽

Mechanical Interlocking ◽

Surface Adhesion ◽

Low Oxygen ◽

Aromatic Rings ◽

Adhesive Properties ◽

End Groups ◽

Hydrolysis Of

AbstractThe adhesive properties of PET have been enhanced by treatments with alkaline solutions and CF4/02 plasmas. Alkaline solution induce hydrolysis of the polyester and etching, with consequent strong roughening of the surface. Adhesion siemed more related to etching than to surface chemistry modifications, suggesting a prominent role of mechanical interlocking.CF4/02 plasmas did not induce significant etching, but the surface chemistry was strongly altered. Ester bridges were effectively removed by the plasma, high oxygen mixtures destroyed aromatic rings and introduced oxygen by the hydroperoxide mechanism. Adhesion was worsened by the introduction of fluorocarbon radicals by Low oxygen discharges. The most effective enhancement was obtained with the 30 % discharge, where the aromatic rings are still quite intact, while ester bridges are removed with formation of phenolic end groups.

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Computer Reconstruction of the Cellular Architecture of the Daphnia Magna Optic Ganglion

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100115043 ◽

1975 ◽

Vol 33 ◽

pp. 284-285

Author(s):

E. R. Macagno ◽

C. Levinthal

Keyword(s):

Daphnia Magna ◽

Genetic Background ◽

Compound Eye ◽

Synaptic Connectivity ◽

Serial Sections ◽

Cross Sectional ◽

Small Crustacean ◽

Optic Ganglion ◽

Structural Invariance ◽

High Degree

The optic ganglion of Daphnia Magna, a small crustacean that reproduces parthenogenetically contains about three hundred neurons: 110 neurons in the Lamina or anterior region and about 190 neurons in the Medulla or posterior region. The ganglion lies in the midplane of the organism and shows a high degree of left-right symmetry in its structures. The Lamina neurons form the first projection of the visual output from 176 retinula cells in the compound eye. In order to answer questions about structural invariance under constant genetic background, we have begun to reconstruct in detail the morphology and synaptic connectivity of various neurons in this ganglion from electron micrographs of serial sections (1). The ganglion is sectioned in a dorso-ventra1 direction so as to minimize the cross-sectional area photographed in each section. This area is about 60 μm x 120 μm, and hence most of the ganglion fit in a single 70 mm micrograph at the lowest magnification (685x) available on our Zeiss EM9-S.

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