Preliminary Cytotoxic Activity of Sutherlandia frutescens and Carpobrotus edulis on Malignant glioblastoma Cells

Lemon balm (Melissa officinalisL.) has many biological effects but especially important is its neuroprotective activity. The aim of the study is to produce different extracts ofMelissa officinalisand analyse their chemical composition and biological properties on rat glioblastoma C6 cells. Results revealed that rosmarinic acid (RA) is the predominant compound of lemon balm extracts. RA has cytotoxic effect on glioblastoma cells (LC50290.5 μM after the incubation of 24 h and LC50171.3 μM after 48 h). RA at concentration 80–130 μM suppresses the cell proliferation and has an antioxidant effect. 200 μM and higher concentrations of RA have a prooxidant effect and initiate cell death through necrosis. The aqueous extract of lemon balm is also enriched in phenolic compounds: protocatechuic, caftaric, caffeic, ferulic, and cichoric acids and flavonoid luteolin-7-glucoside. This extract at concentrations 50 μM–200 μM RA has cytotoxic activity and initiates cell death through apoptosis. Extracts prepared with 70% ethanol contain the biggest amount of active compounds. These extracts have the highest cytotoxic activity on glioblastoma cells. They initiate generation of intracellular ROS and cell death through apoptosis and necrosis. Our data suggest that differently prepared lemon balm extracts differently affect glioblastoma cells and can be used as neuroprotective agents in several therapeutic strategies.

Download Full-text

INVOLVEMENT OF PERFORIN/GRANZYME B-DEPENDENT SIGNALING PATHWAY IN CYTOTOXIC ACTIVITY OF DENDRITIC CELLS TOWARDS HUMAN GLIOBLASTOMA CELLS

Medical Immunology (Russia) ◽

10.15789/1563-0625-2017-4-421-430 ◽

2017 ◽

Vol 19 (4) ◽

pp. 421-430

Author(s):

T. V. Tyrinova ◽

O. Yu. Leplina ◽

S. V. Mishinov ◽

M. A. Tikhonova ◽

A. V. Kalinovskiy ◽

...

Keyword(s):

Dendritic Cells ◽

Cytotoxic Activity ◽

Signaling Pathway ◽

Granzyme B ◽

Glioblastoma Cells ◽

Human Glioblastoma ◽

Human Glioblastoma Cells ◽

Dependent Signaling Pathway

Download Full-text

Cytotoxic activity of the MK2 inhibitor CMPD1 in glioblastoma cells is independent of MK2

Cell Death Discovery ◽

10.1038/cddiscovery.2015.28 ◽

2015 ◽

Vol 1 (1) ◽

Cited By ~ 10

Author(s):

FMS Gurgis ◽

MC Åkerfeldt ◽

B Heng ◽

C Wong ◽

S Adams ◽

...

Keyword(s):

Cytotoxic Activity ◽

Glioblastoma Cells

Download Full-text

A Study of The Assessment for Boron Neutron Capture Therapy Agent

Indonesian Journal of Physics and Nuclear Applications ◽

10.24246/ijpna.v2i1.9-19 ◽

2017 ◽

Vol 2 (1) ◽

pp. 9

Author(s):

Isman Mulyadi Triatmoko ◽

Sutjipto Sutjipto

Keyword(s):

Cytotoxic Activity ◽

Neutron Irradiation ◽

Boron Neutron Capture Therapy ◽

Neutron Capture ◽

Neutron Capture Therapy ◽

Glioblastoma Cells ◽

Morphological Alterations ◽

Boron Neutron Capture ◽

Icp Ms ◽

Biological Effectiveness

A study of the assessment criteria covers the synthesis and characterization of agent and test their biological effectiveness as boron neutron capture therapy (BNCT) agents in cancer treatment. The cellular uptake of this agent into the glioblastoma cells was assessed by boron analysis (ICP-MS) and by fluorescence imaging (confocal microscopy). The agent enters the glioblastoma cells exhibiting a similar profile, i.e., preferential accumulation in the cytoskeleton and membranes and a low cytotoxic activity (IC50 values higher than 200 μM). The cytotoxic activity and cellular morphological alterations after neutron irradiation in the Research Reactor (>107 neutrons cm−2 s−1) were assessed by the MTT assay and by electron microscopy (TEM). Post neutron irradiation revealed that BNCT has a higher cytotoxic effect on the glioblastoma cells. Results provide a strong rationale for considering one of these compounds as a lead candidate for a new BNCT agent.

Download Full-text

Defective Regulation of Membrane TNFα Expression in Dendritic Cells of Glioblastoma Patients Leads to the Impairment of Cytotoxic Activity against Autologous Tumor Cells

International Journal of Molecular Sciences ◽

10.3390/ijms21082898 ◽

2020 ◽

Vol 21 (8) ◽

pp. 2898

Author(s):

Tamara Tyrinova ◽

Olga Leplina ◽

Sergey Mishinov ◽

Marina Tikhonova ◽

Evgeniya Dolgova ◽

...

Keyword(s):

Dendritic Cells ◽

Cytotoxic Activity ◽

Tumor Cells ◽

Tumor Cell Line ◽

Autologous Tumor ◽

Glioblastoma Cells ◽

Tumoricidal Activity ◽

Killer Activity ◽

Double Stranded Dna ◽

Antigen Presenting

Besides an antigen-presenting function and ability to induce antitumor immune responses, dendritic cells (DCs) possess a direct tumoricidal activity. We previously reported that monocyte-derived IFNα-induced DCs (IFN-DCs) of glioblastoma multiforme patients express low levels of membrane TNFα molecule (mTNFα) and have impaired TNFα/TNF-R1-mediated cytotoxicity against immortalized tumor cell line HEp-2. However, whether the observed defect could affect killer activity of glioma patient DCs against autologous tumor cells remained unclear. Here, we show that donor IFN-DCs possess cytotoxic activity against glioblastoma cell lines derived from a primary tumor culture. Granule-mediated and TNFα/TNF-R1-dependent pathways were established as the main mechanisms underlying cytotoxic activity of IFN-DCs. Glioblastoma patient IFN-DCs showed lower cytotoxicity against autologous glioblastoma cells sensitive to TNFα/TNFR1-mediated lysis, which was associated with low TNFα mRNA expression and high TACE/ADAM-17 enzyme activity. Recombinant IL-2 (rIL-2) and human double-stranded DNA (dsDNA) increased 1.5-fold cytotoxic activity of patient IFN-DCs against autologous glioblastoma cells. dsDNA, but not rIL-2, enhanced the expression of TNFα mRNA and decreased expression and activity of TACE/ADAM-17 enzyme. In addition, dsDNA and rIL-2 stimulated the expression of perforin and granzyme B (in the presence of dsDNA), suggesting the possibility of enhancing DC cytotoxicity against autologous glioblastoma cells via various mechanisms.

Download Full-text