scholarly journals Antioxidants properties of Murraya koenigii: a comparative study of three different extraction methods

Food Research ◽  
2020 ◽  
Vol 5 (1) ◽  
pp. 43-49
Author(s):  
M.T. Parithy ◽  
Mohd Zin Z. ◽  
Hasmadi M. ◽  
N.D. Rusli ◽  
K.L. Smedley ◽  
...  
Keyword(s):  
Total Phenolic Content ◽  
High Performance ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Thiobarbituric Acid ◽  
Extraction Methods ◽  
Total Phenolic ◽  
Assisted Extraction ◽  
Pharmaceutical Industries

Nutraceutical and pharmaceutical industries have been increasingly engaged in finding natural alternatives compounds as potential antioxidants. The use of phytochemicals is introduced as a good source of natural antioxidants. Murayya koenigii leaves, commonly used in cooking and traditional medicines have been examined for their remarkable antioxidant potential, yet still, it remains an understudied herb. Therefore, this study aimed to determine the antioxidant properties and flavonoids profile in M. koenigii leaves extracted using; solvent assisted extraction (SAE), microwave assisted extraction (MAE) and ultrasonic assisted extraction (UAE). The antioxidant properties of M. koenigii were analysed qualitatively and quantitatively using high performance liquid chromatography (HPLC). M. koenigii leaves extracted using the UAE method have responded strongly towards a 2, 2-diphenyl -2-picryl-hydrazyl DPPH assay with the highest inhibition (%) of 78.00±1.00. Using the ferric thiocyanate (FTC) and thiobarbituric acid (TBA) method assays, the M. koenigii leaves with the lowest absorbance were assigned as a sample with the highest antioxidant activity. The M. koenigii leaves extracted using UAE had the lowest absorbance with 0.01±0.00. In the TPC assay, the MAE method showed the highest total phenolic content (120.60±14.81 mg GAE/g sample). The TFC assay demonstrated that MAE methods have the highest total phenolic content (93.38±4.33 mg QE/g sample. The M. koenigii leaves extracted by MAE showed the highest gallic acid, catechin, epigallocatechin gallate, rutin and kaempferol concentration (mg/L). M. koenigii leaves subjected to SAE extraction has the highest concentration of p-coumaric acid, myricetin and quercetin (mg/L). This study found that M. koenigii leaves extracted using UAE exhibited better antioxidant activities than that of MAE and SAE. These useful findings have managed to narrow the knowledge gap regarding the effects of different extraction methods on the antioxidant property of M. koenigii.

scholarly journals Comparative Evaluation of Antioxidant Properties and Isoflavones of Tempeh Fermented in Two Different Wrapping Materials

2018 ◽  
Vol 6 (2) ◽  
pp. 307-317 ◽  
Author(s):  
Normah Hashim ◽  
Chia Woon Tai Woon Tai ◽  
Hui Xin Wen Xin Wen ◽  
Amin Ismail ◽  
Kin Weng Kong
Keyword(s):  
Total Phenolic Content ◽  
High Performance ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Health Benefit ◽  
Total Phenolic ◽  
Soybean Food ◽  
Banana Leaf

Tempeh is an Indonesian traditional fermented soybean food that is well known for its nutritional value and health benefit. This study aimed to evaluate the effects of two commonly used packaging materials in tempeh production, namely banana leaf and polyethylene bag, on the antioxidant properties. Isoflavones, include daidzein and genistein, of tempeh fermented in banana leaf (TBL) and polyethylene bag (TP) were analysed with high-performance liquid chromatography. Total phenolic content and antioxidant activities were evaluated using Folin-Ciocalteu reagent, DPPH radical scavenging and β-carotene-linoleate bleaching assays, respectively. The changes of antioxidant properties of tempeh after different fermentation periods in both wrapping materials were observed, on day 0 (unfermented), day 1, day 2 and day 4. Total phenolic content, daidzein, genistein and the antioxidant activities were significantly (p < 0.05) increased in both TBL and TP as the fermentation progressed. A higher total phenolic content was found in TBL on day 1 and day 2 but TP exhibited the highest total phenolic content on day 4. Daidzein was the highest in TBL on day 2 but it was reduced on day 4 while genistein was the highest in TP on day 4. Antioxidant activities were positively (r = 0.272–0.969, p < 0.05) correlated to total phenolic content, daidzein and genistein. Although tempeh wrapped in banana leaf is a traditional way for tempeh packaging, it provided better antioxidant properties on day 1 and day 2, which is within the best period of tempeh consumption. Hence, the current findings provided useful information to manufacturer and consumer in selecting the suitable wrapping material for tempeh with optimal phytonutrients.

scholarly journals Antioxidant Activities and Total Phenolic Content of Malaysian Herbs as Components of Active Packaging Film in Beef Patties

Antioxidants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 204 ◽  
Author(s):  
Wan Amnin Wan Yahaya ◽  
Noraziah Abu Yazid ◽  
Nurul Aini Mohd Azman ◽  
María Pilar Almajano
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Thiobarbituric Acid ◽  
Active Packaging ◽  
Total Phenolic ◽  
Thiobarbituric Acid Reactive Substance ◽  
Packaging Film

Active packaging containing natural extracts is a promising innovation to prolong the shelf life of perishable food. The objective of this work was to develop a bioactive edible film from semi-refined carrageenan (SRC) and glycerol (G) as plasticizer incorporated with natural extract. Five Malaysian herbs were evaluated to determine their total phenolic content (TPC) and antioxidant activities. The Persicaria minor (PM) extract demonstrated the highest TPC (1.629 mg GAE/L sample) and radical scavenging activity evaluated by the radicals 2,2’-azinobis [3-ethylbenzothiazoline-6-sulfonic acid] (27.166 mg TE/L sample), 2,2-diphenyl-1-picrylhydrazyl (719.89 mg eq. Trolox/L sample) and α,α′-Azodiisobutyramidine dihydrochloride (5.81 mg TE/L sample). Thus, PM extract was selected for active packaging film at concentrations of 0.4, 1.0 and 2.0% and compared with 0.4% Butylatedhydroxianisole in 2% SRC and 0.9% G film formulation. The meat patties were wrapped in the films and stored under refrigeration (4 ± 2 °C) for 14 days. The film with 2% PM exhibited significantly lower lipid deterioration analysed by the thiobarbituric acid reactive substance assay (p < 0.05) and small changes in % metmyoglobin value which indicated the minimum development of brown colour (p < 0.05). Hence, this film can be used as a packaging material to improve meat quality characteristics.

Optimization of Total Phenol and Tannin Content and Biological Activity of Dittrichia graveolens (L.) GREUTER.

2020 ◽  
Vol 16 (2) ◽  
pp. 124-132 ◽  
Author(s):  
Mahsa Souri ◽  
Alireza Shakeri
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Antibacterial Activities ◽  
Methanol Concentration ◽  
Total Phenolic ◽  
Tannin Content ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Background: Dittrichia graveolens (L.) is a strongly aromatic plant distributed in Mediterranean regions. This research concerns the optimization of the Total Phenolic Content (TPC) and the Total Tannin Content (TTC) of Dittrichia graveolens (L.) extracts using Response Surface Methodology for Ultrasound Assisted Extraction (UAE) and Microwave Assisted Extraction (MAE). Central Composite Design (CCD) was used to evaluate the effect of the solvent concentration and the extraction time, in different methods on TTC and TPC. The antioxidant activities and antibacterial activities were evaluated. Methods: The plant extracts were prepared using maceration, microwave and ultrasound assisted extraction. TPC and TTC were measured using Folin-Ciocalteu method. The antioxidant activities were studied using DPPH reagent and disc diffusion method was used to study the antibacterial activities. Results: This study showed the optimum condition for UAE was 49.96% methanol concentration and 11.2 min sonication, while for MAE was 55.44% methanol concentration and 2.26 min microwave extraction. It also indicated that MAE was the most effective method in comparison to UAE and maceration. The antioxidant activities of MAE extract (IC50=7.7mg/mL) were more than UAE extract (IC50=21.5mg/mL) and maceration (IC50=32.3mg/mL). Conclusion: As a conclusion, it was indicated that MAE was the most effective method. The higher total phenolic content caused higher antioxidant activities as MAE extract had the highest antioxidant activities. The antibacterial test showed the great potential of this plant as an antibacterial compound resource against different bacteria.

scholarly journals Preservation of the Polyphenolic Content and Antioxidant Properties of Rubus Glaucus Benth

2019 ◽  
Vol 7 (3) ◽  
pp. 886-893
Author(s):  
Johana Velásquez Castro ◽  
Juan Pablo Arrubla Vélez ◽  
Gloria Edith Guerrero Álvarez ◽  
Nathalia Cardona Hurtado
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Low Cost ◽  
Antioxidant Properties ◽  
Aloe Vera ◽  
Total Phenolic ◽  
Balanced Diet ◽  
The Andes ◽  
Nutraceutical Properties

The Andes berry (Rubus glaucus Benth) has an ongoing production in Colombia and is considered a competitive market product with the potential for exportation and broad applications in agroindustry because of its high content of antioxidants, nutrients, and proteins, which are keys to a balanced diet. In this study, the effect of an Aloe vera gel coating on the preservation of the nutraceutical properties of the Andes berry was evaluated by measuring the antioxidant capacity and the total phenolic content of the fruit using the DPPH (2, 2-diphenyl-1-picrylhydrazyl) and Folin-Ciocalteu methods, respectively. The antioxidant activities in fruits without and with coating were 95.14 ± 3.28 mmol AAE 100 g-1 sample and 135.94 ± 1.09 mmol AAE 100 g-1 sample, respectively, after ten day of storage. The total phenolic content of the fruits with and without coating did not present significant differences, i.e., the phenol concentrations remained constant over time. Implementation of the gel coating is recommended as a low-cost and highly available option for the preservation of the Andes berry and its nutraceutical properties.

scholarly journals Antioxidant Properties of Western Georgia Native Khechechuri Pear

Sci ◽  
2020 ◽  
Vol 2 (2) ◽  
pp. 31
Author(s):  
Tamara Gabour Sad ◽  
Indira Djafaridze ◽  
Aleko Kalandia ◽  
Maia Vanidze ◽  
Katarina Smilkov ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Total Phenolic Content ◽  
Fruit Juice ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Bioactive Components

An endemic pear species spreads in one region of western Georgia, Adjara, called Khechechuri. Pears are dietary source of bioactive components such as polyphenols and triterpenic acid. In addition to its gastronomic value, the aim of the article was to examine and compare phenolic compounds, flavonoids, catechins, phenolic acids, and antioxidant activities in five species of Khechechuri collected from various regions in Adjara region: Adjaristskali, Merisi, Dandalo, Shuakhevi, and Khulo. Five fruit parts, the skin, edible pulp, the whole pear (skin + pulp), juice and the pomace were analyzed and the results were compared. Our study revealed that the amount of total phenolic content found in the skin of West Georgian pear types was as much as 4650 mg/kg. Also, the pear pomace showed significant amount of total phenolic content in all species of Khechechuri. In addition, in all species of Khechechuri pears, flavonoids were found, except in the fruit juice.

scholarly journals Assessment of Total Phenolic Content and Antioxidant Activity Potential of Clementine Extract Obtained by Microwave Assisted Extraction Method

2021 ◽  
Vol 18 (4) ◽  
pp. 779-785
Author(s):  
Hanane Azzouzi ◽  
Loubna Elhajji ◽  
Mouad Achchoub ◽  
Mohammed Benbati ◽  
Kaoutar El-Fazazi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Extraction Method ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Major Interest ◽  
Pharmaceutical Industries

Citrus peels are known as rich source of bioactive compounds. Therefore, the establishment of an appropriate and innovative extraction method is a major interest to the food processing industry. The aim of this work is to assess the total phenolic content (TPC) and antioxidant activity (AA) of peels extracts prepared from five Moroccan clementine varieties. Extracts were prepared with microwave-assisted extraction (MAE) technique using acetone and methanol solvents. The evaluation of the antioxidant activity was performed by DPPH and ABTS- free radical scavenging. The extraction conditions had significant effects on total phenolic content (TPC) and antioxidant activity (AA). In fact, the highest TPC amount of clementine varieties (730.850±0.004mg GAE/100g DM) was obtained with acetone solvent for the Sidi Aissa variety. Regarding antioxidant activity, results with DPPH method showed an important antiradical and antioxidant activities for Sidi Aissa variety with methanol solvent (46.30± 1.01%) . However, ABTS method showed a good antioxidant activity for Ain Taoujdat with methanol solvent (3.02 mg/100g DM). This work provided to study the biochemical quality of Moroccan clementine extracts obtained by two microwave-assisted extraction processes in order to investigate further industrial potential uses in agro-food and pharmaceutical industries

scholarly journals Investigation of biochemical characters and antioxidant properties of different winged bean (Psophocarpus tetragonolobus) genotypes grown in Indonesia

2020 ◽  
Vol 21 (6) ◽  
Author(s):  
JANSON CALVINDI ◽  
MUHAMAD SYUKUR ◽  
Waras Nurcholis
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Winged Bean ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Psophocarpus Tetragonolobus ◽  
Fresh Weight ◽  
Antioxidant Power

Abstract. Calvindi J, Syukur M, Nurcholis W. 2020. Investigation of biochemical characters and antioxidant properties of different winged bean (Psophocarpus tetragonolobus) genotypes grown in Indonesia. Biodiversitas 21: 2420-2424. Winged bean, Psophocarpus tetragonolobus (L) DC, is described as having antioxidant properties. This work evaluated the biochemical and antioxidant characteristics of the P. tetragonolobus genotypes. Twelve-winged bean genotypes were calculated for total phenolic content (TPC) and total flavonoid content (TFC) of biochemical characters and antioxidant properties by using four methods: 2,2-diphenyl picrylhydrazyl (DPPH), cupric reducing antioxidant power (CUPRAC), ferric reducing antioxidant power (FRAP), and Trolox equivalent antioxidant capacity (TEAC). The plant material was grown in the same location conditions in Indonesia. The total phenolic content varied from 154.6 to 161.5 mg GAE/ 100 g, and flavonoid ranged from 105.2 to 112.4 mg QE/ 100 g fresh weight. The antioxidant capacities were 30.6 - 47.0, 140.4 - 167.6, 66.9 - 170.8, and 28.0 - 52.4 µmol TE/ 100 g fresh weight as calculated by the DPPH, TEAC, FRAP, and CUPRAC assays, respectively. The antioxidant activities were significantly correlated with the polyphenol content of winged bean genotypes fruits. The genotypes TU, L3, H3U, H1P, and TH were recognized higher based on their TPC, TFC, and antioxidant activities, indicating that these genotypes to be promising for further breeding program and commercial purposes.

Antioxidant Properties of Western Georgia Native Khechechuri Pear

Sci ◽  
2019 ◽  
Vol 1 (2) ◽  
pp. 44
Author(s):  
Tamara Gabour Sad ◽  
Indira Djafaridze ◽  
Aleko Kalandia ◽  
Maia Vanidze ◽  
Katarina Smilkov ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Total Phenolic Content ◽  
Fruit Juice ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Bioactive Components

An endemic pear species spreads in one region of western Georgia, Adjara, called Khechechuri. Pears are dietary source of bioactive components such as polyphenols and triterpenic acid. In addition to its gastronomic value, the aim of the article was to examine and compare phenolic compounds, flavonoids, catechins, phenolic acids, and antioxidant activities in five species of Khechechuri collected from various regions in Adjara region: Adjaristskali, Merisi, Dandalo, Shuakhevi, and Khulo. Five fruit parts, the skin, edible pulp, the whole pear (skin + pulp), juice and the pomace were analyzed and the results were compared. Our study revealed that the amount of total phenolic content found in the skin of West Georgian pear types was as much as 4650 mg/kg. Also, the pear pomace showed significant amount of total phenolic content in all species of Khechechuri. In addition, in all species of Khechechuri pears, flavonoids were found, except in the fruit juice.

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