scholarly journals Total Phenolic Contents and Antioxidant Activities of Different Herbal Tea Extracts from Turkey

2019 ◽  
Vol 3 (2) ◽  
pp. 66-72
Author(s):  
Keyword(s):  
Green Tea ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Hot Water ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Herbal Tea ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Dpph Radical Scavenging

: The aim of the present study is to determine the total phenolic contents (TPC), DPPH radical scavenging activities, and reducing power of commercially consumed herbal teas including sage tea (ST), blackberry tea (BT), apple tea (AT), Echinacea tea (ET), lime tea (LT), rosehip tea (RT), and green tea (GT) in Turkey. For this purpose, various solvents were used for extraction of the tea samples and the results were compared in terms of total phenolic contents and antioxidant activities, as well. Hot water is found as the best solvent for the extraction of total phenolics from ST, BT, AT, and RT, respectively. On the other hand, the highest TPC values are obtained with the extraction of ET, LT, and GT in the presence of aqueous acetone (50%) solvent system. Generally all samples gave high DPPH radical scavenging activities depending on their extraction solvents. The highest radical scavenging activity was found as 94.92 ± 0.01 % for ST in ethanol, while the lowest activity was obtained as 5.98 ± 0.14 % for BT with water extract. Ferric reducing antioxidant assay results are closed each other and the highest activity (0.27 ± 0.74 mM Trolox) was observed in the ethanol extract of ST. Antioxidant activities and TPC of the herbal tea samples varied depending on the extraction solvent. Water or aqueous organic solvent extracts of sage tea and green tea samples gave high TPC values and antioxidant activities.

Antimicrobial activities of Allium staticiforme and Allium subhirsutum

2020 ◽  
Vol 15 (1) ◽  
pp. 19-23
Author(s):  
Alican Bahadir Semerci ◽  
Dilek İnceçayır ◽  
Vusale Mammadova ◽  
Ayşegül Hoş ◽  
Kenan Tunç
Keyword(s):  
Radical Scavenging ◽  
Activity Level ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Antifungal Activities ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Methanolic Extracts ◽  
Dpph Radical Scavenging

The antibacterial and antifungal activities of the bulb and flower of Allium staticiforme and Allium subhirsutum were investigated. In addition, DPPH radical scavenging activity and total phenolic contents were determined. The results show that methanolic extracts of A. staticiforme and A. subhirsutum had antifungal activities against Candida albicans, together with a less activity level against Escherichia coli, Staphylococcus epidermidis, S. aureus, Enterecoccus faecalis, Salmonella typhimurium and Pseudomanas aeruginosa. The total phenolic contents of A. staticiforme leaf and bulb were determined as 17 and 2.4 mg of GAE/100 g, respectively. The IC50 of methanolic extracts of A. staticiforme and A. subhirsutum were also determined. In conclusion, both A. staticiforme and A. subhirsutum have antifungal activities with weak antibacterial activities. These plants have DPPH radical scavenging activities. Video Clip of Methodology: Disc Diffusion Method: 3 min 04 sec:  Full Screen   Alternate

scholarly journals Chemical Composition and Antioxidant Activity of the Essential Oil and Fatty Acids of the Flowers of Rhanterium Adpressum

2013 ◽  
Vol 8 (8) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Chahrazed Hamia ◽  
Nadhir Gourine ◽  
Hadjer Boussoussa ◽  
Mokhtar Saidi ◽  
Emile M. Gaydou ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Lipid Extract ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Oil Extract

The essential oil obtained by hydrodistillation of the flowers of Rhanterium adpressum Coss. & Durieu was analyzed using GC and GC-MS. The essential oil was very rich in monoterpene compounds. The major components identified were the monoterpene hydrocarbons: camphene (21.8%), myrcene (19.3%) and α-pinene (17.4%). Other compounds, including limonene, β-pinene and terpinol-4-ol, were present in low content (4–6%). The composition of the fatty acids in the lipid extract obtained from the flowers was also investigated by GC and GC-MS. The main fatty acids identified were palmitic (47.4%), oleic (12.9%) and stearic acids (10.6%). The total phenolic contents and the antioxidant activities were also evaluated for both extracts. The total phenolic contents were determined using the Folin-Ciocalteu reagent and the antioxidant activities were measured using three different assays: DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity, FRAP (ferric reducing antioxidant potential) and a molybdenum assay. As a result of these tests, the lipid extract exhibited the highest antioxidant activities in comparison with the essential oil extract.

scholarly journals Comparative Studies on Anti-Inflammatory, Antioxidant and Antimutagenic Activities of Crassocephalum crepidioides (Bent) Leaf Cold and Hot Water Extracts

2019 ◽  
pp. 1-12 ◽  
Author(s):  
B. A. Akinpelu ◽  
A. Godwin ◽  
T. Gbadegesin ◽  
N. Ajakaye ◽  
S. E. Omotosho ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Radical Scavenging ◽  
Hot Water ◽  
Dpph Radical ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

Aim: To investigate the anti-inflammatory, anti-oxidant and genotoxicity activities of Crassocephalum crepidioides leaf. Study Design: Comparative investigations of the medicinal value and toxicity profile of cold water (CW) and hot water (HW) extracts of C. crepidioides leaf. Place and Duration of Study: Biochemistry and Molecular Biology Department, Obafemi Awolowo University, Ile-Ife. January 2015-October 2016. Materials and Methods: CW and HW of C. crepidioides were analyzed for anti-inflammatory activity via red blood cell membrane stabilization technique and in vitro methods using DPPH radical scavenging activity, thiobarbituric acid-reactive substances (TBARS), ferric reducing antioxidant power (FRAP) and inhibition of oxidative haemolysis were employed to evaluate the antioxidant property. Allium cepa chromosomal assay was adopted to investigate the genotoxic effect of the extracts. Total flavonoid and phenolic contents of the extracts were estimated spectrophotometrically. Results: Both extracts stabilized stressed red blood cell membranes with maximum percentage stability of 50.97±0.06 and 90.90±0.02 at 0.5 and 2.0 mg/ml for CW and HW extracts respectively. The CW extract elicited no DPPH radical scavenging (IC50 -0.63±0.02 mg/ml) and lipid peroxidation (IC50 -0.32±0.00) activities. HW extract had IC50 of 0.29±0.02 and 0.17±0.00 mg/ml for DPPH and lipid peroxidation. CW and HW extracts exhibited FRAP activity of 1186.96±0.01 and 1015.54±0.01 µmol AAE/g respectively. CW extract displayed a weaker protection (29.01±0.01%) against oxidative haemolysis compared to HW extract (68.70 ± 0.00%). CW extract contained higher phenolic contents (2.16±0.03 µmolGAE/g extract) while the HW extract contained higher flavonoids (0.61±0.05 µmolQE/g extract). CW and HW extracts inhibited A. cepa root growth to 71.40±0.02 and 59.10±0.02% respectively. A. cepa mitotic index was reduced to 8.85±0.01 and 8.67±0.02 for CW and HW extracts as compared with control (26.62%). Conclusion: The study concluded that consumption of C. crepidioides leaf in cooked form has more medicinal values however, both CW and HW extracts are capable of causing cellular damage at high doses.

scholarly journals Effects of Solvents on Total Phenolic Content and Antioxidant Activity of Ginger Extracts

2020 ◽  
Author(s):  
Dessie Ezez ◽  
Molla Tefera
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Acetone Extract ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Abstract Ginger (Zingiber officinale) is a popular spice which used for the treatment of different gastrointestinal and inflammatory discomfort. In the present study, the total phenolic content (TPC) and antioxidant activity of ginger extract using four solvents (ethanol, methanol, acetone and ethyl acetate) were determined. Among the four solvents, methanol extract showed that the maximum phenolic (1183.813 mg GAE/100 g at Ayikel and 1022.409 mg GAE/100 g at Mandura) and the least were found in acetone extract (748.865 mg GAE/100 g at Ayikel and 690.152 mg GAE/100 g at Mandura). In addition, the highest DPPH radical scavenging activity (84.868% at Ayikel and 82.883% at Mandura) was observed in methanol. However, acetone showed the least DPPH radical scavenging activity (73.864% at Ayikel and 70.597% at Mandura). Antioxidant activities of ginger extracts were also expressed as IC50 values and acetone extract has maximum IC50 value (0.654 and 0.812 mg/mL) followed by ethyl acetate and ethanol, while the lowest for methanol extracts (0.481 and 0.525 mg/mL). The result of this study showed that extraction solvents significantly affected the total phenolic content and antioxidant activities of ginger. Thus, ginger can be regarded as promising candidates for natural sources of antioxidants with high value of phenolic contents.

Micropropagation and Evaluation of Phenolic Content, Antioxidant and Antimicrobial Activities of Vernonia cinerea (Linn.) Less

2015 ◽  
Vol 19 ◽  
pp. 36-41
Author(s):  
MA Sonibare ◽  
TO Aremu ◽  
PA Okorie
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Wild Plant ◽  
Total Phenolic ◽  
Ms Medium ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Antioxidant Agents ◽  
Vernonia Cinerea

Vernonia cinerea, belonging to the family Asteraceae, is of wide medicinal application. This study investigated the antimicrobial, antioxidant activities and the total phenolic contents of wild plant of Vernonia cinerea with its respective shoot cultures.Nodal explants of V. cinerea were cultured on Murashige and Skoog (MS) medium supplemented with various combinations of BAP (0.5-2.5 mg/L) and NAA (0.1-0.5 mg/L) including various concentrations of Gibberllic acid for subculturing. The antimicrobial activity of plant extracts was tested using agar well diffusion and macrodilution methods for zone of inhibition (ZI), minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).The free radical scavenging test was conducted using 2,2, diphenyl-1-picryl hydrazyl (DPPH) reagent while total phenolic contents (TPC) were measured by Folin–Ciocalteau reagent. Maximum shoot proliferation of 11.42 ± 2.26 cm was obtained from MS medium supplemented with 0.5 mg/L BAP + 0.1 mg/L NAA. All extracts displayed moderate antimicrobial potential against the tested pathogens in the range of 9.0-13.1 mm ZI, with highest MIC of 0.78 mg/mL and 1.56 mg/mL fromMPE and WPE, respectively.The IC50 values of 4.49 and 5.10 μg/mL was obtained in WPE and MPE. The MPE had TPC of 6.66 ± 0.83 mg GAE/g compared to the WPE with TPC of 5.43 ± 1.31 mg GAE/g. The crude methanol extracts of wild and micropropagated plants of V. Cinerea showed high amounts of phenolic compounds, which could present them as candidates for future search for antimicrobial and antioxidant agents for different ailments.Keywords: Micropropagation, Antioxidant, DPPH, Phenolic Compound, Vernonia cinerea

scholarly journals Antioxidative and Quality Properties of Full-Fat Date Seeds Brew as Influenced by the Roasting Conditions

Antioxidants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 226 ◽  
Author(s):  
Mohammad Fikry ◽  
Yus Aniza Yusof ◽  
Alhussein M. Al-Awaadh ◽  
Russly Abdul Rahman ◽  
Nyuk Ling Chin ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Browning Index ◽  
Phenolic Contents ◽  
Dpph Radical Scavenging

Full-fat roasted date seeds are considered an excellent source of antioxidants which can treat many diseases. The specific objectives were to investigate the effect of roasting temperature and time on the hardness of whole seeds, moisture content of the roasted date seeds powder, DPPH radical scavenging activity, total phenolic contents, extraction yield, pH, browning index and sensory properties of the brew prepared from the full-fat roasted date seeds and to construct descriptive models that could describe this effect. Date seeds were roasted at three temperatures (160, 180 and 200 °C) for different period of times (10, 20 and 30 min) using a natural conventional oven; then grinded and next brewed. Hardness of whole seeds, moisture content of the seeds powder, DPPH radical scavenging activity and total phenolic contents, extraction yield, pH and browning index and sensory properties of the brew were significantly affected by the roasting conditions. The statistical results indicated that the proposed model could adequately describe the measured properties. Strong correlations have been found among the properties of the brew as well. The producers of the date seeds brew can utilize these results for controlling the roasting process.

scholarly journals The Antioxidation Effect of Brown Algae Extract

2021 ◽  
Vol 27 (4) ◽  
pp. 851-858
Author(s):  
Sook Hee Shin ◽  
Sang Mo Kang
Keyword(s):  
Ethanol Concentration ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Dpph Radical Scavenging Activity ◽  
Phenolic Contents ◽  
Cosmetic Ingredients ◽  
Dpph Radical Scavenging

This study attempted to investigate the functionality of <i>Dactylosiphon bullosus</i>, <i>Sporochnus radiciformis</i>, and <i>Zonaria diesingiana</i> extracts among algae as cosmetic ingredients. For this, antioxidant experiments were performed. <i>D. bullosus</i>, <i>S. radiciformis</i> and <i>Z. diesingiana</i> extracts were prepared with a 70% ethanol concentration to measure antioxidant activities. In terms of DPPH radical scavenging activity, <i>Z. diesingiana</i> was the highest with 94.20±0.28%. In ABTS radical scavenging activity, <i>Z. diesingiana</i> was the highest with 96.26±0.98%. In terms of the total phenolic contents and NO radical scavenging activity, <i>Z. diesingiana</i> was the highest with 47.19±2.07 mg gallic acid/g and 92.87±1.79%, respectively. The above results show that <i>Z. diesingiana</i> extract has the greatest antioxidant activity, confirming its potential as a cosmetic ingredient.

scholarly journals Comparison of Total Phenolic Contents and Antioxidant Activities of Centella asiatica Extracts Obtained by Three Extraction Techniques

2019 ◽  
Vol 6 (2) ◽  
pp. 42-49
Author(s):  
Wan Nurul Huda Wan Zainal ◽  
FARA RESTINA MUSAHIB ◽  
NABILAH SYAFIQAH ZULKEFLEE
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Centella Asiatica ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Extraction Techniques ◽  
Phenolic Contents ◽  
Total Phenolic Contents

Through different extraction techniques, the potential of Centella asiatica as a natural source of antioxidant was investigated. The C. asiatica aqueous extracts were obtained via infusion, decoction and ultrasonic-assisted extraction (UAE) techniques. The effects of different extraction techniques were studied on the extraction yield, total phenolic contents and antioxidant activity. The total phenolic contents of the extracts and antioxidant activity were examined using the Folin-Ciocalteu’s reagent and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay, respectively. Results indicated that the UAE exhibited the highest extraction yield, highest total phenolic contents, as well as highest antioxidant activity. The yield of the extracts increased in the order of infusion < decoction < UAE which were 18.2 %, 23.6% and 25.4 %, respectively. All extraction techniques had a significant effect (p <0.05) on the total phenolic contents and antioxidant activity of C. asiatica extracts. The total phenolic contents was ranged from 3.42 ± 0.030 to 8.32 ± 0.105 mg GAE/g dry extract while the antioxidant activity was in the range of 75 to 86 %. This study confirms that C. asiatica has the potential to be a good resource for the future development of natural antioxidant. In addition, extraction via UAE can be an ideal technique to obtain phytochemical-rich extracts from medicinal plants.

scholarly journals Phenolic compounds and antioxidant activities of grape canes extracts from vineyards

2016 ◽  
Vol 14 (3) ◽  
pp. e0805 ◽  
Author(s):  
Yanlun Ju ◽  
Ang Zhang ◽  
Yulin Fang ◽  
Min Liu ◽  
Xianfang Zhao ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Low Cost ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Methanolic Extracts ◽  
Phenolic Components ◽  
Dpph Radical Scavenging ◽  
Gallic Acid Equivalent

Grape canes are the main agro-wastes from vineyards. This work studied the antioxidant activities of the defatted methanolic extracts (ME) of canes from 11 genotypes: 5 Vitis vinifera widely known cultivars and 6 Chinese wild varieties from three species (V. amurensis, V. davidii, and V. pentagona) and the antioxidant activities of the ME’s chloroform fractions (CF), ethyl acetate fractions (EAF) and water fractions (WF). Among ME and its three fractions, EAF’s total phenolic contents (TPC) and total flavonoid contents (TFC) were the highest, at 586 mg/g of gallic acid equivalent and 320 mg/g of quercetin equivalent, respectively. The antioxidant power of the fractions/extracts was in the order EAF > ME > WF > CF, based on the DPPH radical-scavenging power and ferric-reducing antioxidant activity, while the order was EAF > CF > WF >ME based on the β-carotene-linoleic acid bleaching activity. Methanolic extracts demonstrated the strongest Fe2+-chelating activity. The antioxidant activities of the extracts/fractions generally correlated with the TPC and TFC in all assays, except with the Fe2+-chelating test. Grape canes from V. davidii had the highest TPC, TFC and antioxidant activities compared with those from other grape species. Catechin, epicatechin and trans-resveratrol were the predominant phenolic components of fractions/extracts. In light of these valuable bioactivities, grape canes from annual pruning practice considered as waste material have good commercial potential for utilization as a promising natural antioxidant in the food, pharmaceutical and cosmetic industries, given its low cost and availability in large amounts.

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