scholarly journals Prognostic Value of Serum Concentration of Vascular Endothelial Growth Factor A in Patients in Acute Period of Spontaneous Supratentorial Intracerebral Hemorrhage

2021 ◽  
Vol 6 (2) ◽  
pp. 87-93
Author(s):  
A. A. Kuznietsov ◽  

The aim of the study – to evaluate the informativeness of serum vascular endothelial growth factor A levels in detection of short-term prognosis in acute period of spontaneous supratentorial intracerebral hemorrhage on the ground of conservative treatment. Materials and methods. Prospective cohort comparative study of the 89 patients in acute period of spontaneous supratentorial intracerebral hemorrhage was done on the ground of conservative treatment. The evaluation of neurological deficit level was performed by using Full Outline of Unresponsiveness coma scale, Glasgow scale and National Institute of Health Stroke Scale. Cerebral brain structures visualization was made by computed tomography. During 24 hours of hospitalization serum samples were collected and vascular endothelial growth factor A (VEGF-A) was measures by using ELISA method. Early neurological deterioration and unfavorable acute spontaneous supratentorial intracerebral hemorrhage period outcome (4-6 score by modified Rankin scale on the 21st day of the disease) were registered as combined clinical endpoints. Statistical operation was made with the help of correlation analysis, logistic regression analysis, ROC-analysis. Results. Patients with unfavorable course and spontaneous supratentorial intracerebral hemorrhage acute period outcome were characterized by higher serum VEGF-A values on the 1st day from admission, that correlates with the volume of intracerebral hemorrhage (R=0,21, p˂0,01), volume of secondary intraventricular hemorrhage (R=0,29, p˂0,01) and total volume of intracranial hemorrhage (R=0,31, p˂0,01). It was detected that evaluation of VEGF-A serum concentrations together with neurovisualization parameters (midline shift severity, volume of secondary intraventricular hemorrhage) as a part of multipredictive logistic regression model allow to detect individual risk of early neurological deterioration with the accuracy 89,9% (AUC±SE (95% CI) = 0,91±0,04 (0,83-0,96), р˂0,0001). Conclusions. Serum VEGF-A levels in patients with spontaneous supratentorial intracerebral hemorrhage on the 1st day from admission is an informative additional parameter for prognosis of early neurological deterioration on the ground of conservative treatment

Stroke ◽  
2012 ◽  
Vol 43 (suppl_1) ◽  
Author(s):  
Han-Jin Cui ◽  
Qi-Ting Liu ◽  
Hua-Jun Zhou ◽  
TAO TANG

Background and purpose: Our previous work has proved that Intracerebral hemorrhage (ICH) induces angiogenesis, accompanied by upregulation of vascular endothelial growth factor (VEGF) and its receptors. As the most potent mitogen of endothelial cells (ECs), VEGF must bind to its receptor_vascular endothelial growth factor receptor-2, to make it be phosphorylated, which plays the roles in promoting proliferation, migration, survival of ECs. In this study, our purpose is to investigate the effect of VEGF receptor inhibition on angiogenesis after ICH. Method: Fifteen Kunming mice were randomly divided into 3 groups, including sham control (n=5), ICH group (n=5), and SU5416-treated group (n=5). ICH model was induced by injection collagenase type VII into right globus pallidus stereotaxically. SU5416-treated group was injected by SU5416 (10 mg/kg per dose) intraperitoneally after ICH induction. Intraperitoneal injection of 5-bromo-2'-deoxyuridine (BrdU) was used to label new-born ECs. Neurological severity score (NSS), corner turn test and foot-fault test were used to investigate the neurological function at 1d, 3d and 7d following the surgery. Angiogenesis and the level of p-VEGFR-2 were evaluated at 7d by immunohistochemistry. Results: At 1d, there is no difference between the two ICH-induced groups in NSS, corner turn test, foot-fault test; at 3d and 7d, the NSS in ICH group is significantly lower than that of SU5416-treated group ( P <0.05), and the times for right-turn and foot-fault in ICH group are notably fewer than those of SU5416-treated group ( P <0.05). At 7d, no expression of p-VEGFR-2 was detected in sham control animals, while the expression of p-VEGFR-2 in ICH group is notably higher than that in SU5416-treated group ( P <0.05); and the expression of p-VEGFR-2 is located in vWF-immunoreactive ECs; there is no BrdU-labeled nuclear in vessels observed in sham-control mice, but BrdU-labeled nuclei in vessels in ICH group are remarkably more than those in SU5416-treated group ( P <0.05). Conclusions: Activation of VEGFR-2 is an important mechanism for angiogenesis after ICH, which is related with the recovery of neurological behavioral function.


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