“Gingival Crevicular Fluid Levels of Interleukin-35 in Periodontal Health, Chronic Periodontitis & the Effect of Scaling & Root Planning on Interleukin-35 Levels in Chronic Periodontitis – A Randomized Controlled Clinical Study”

2021 ◽  
Vol 13 (2) ◽  
pp. 7-15
Author(s):  
Dr. Ammu Varghese ◽  
Dr. Harsha Mysore Babu ◽  
Dr. Archana R Naik ◽  
Dr. Savita A M ◽  
Dr. Pallavi Nanaiah Kukkera
Keyword(s):  
Chronic Periodontitis ◽  
T Regulatory Cells ◽  
Gingival Crevicular Fluid ◽  
Scaling And Root Planing ◽  
Regulatory Cells ◽  
Periodontal Health ◽  
Root Planing ◽  
Crevicular Fluid ◽  
Fluid Levels ◽  
Group 1

Background & Objectives: Interleukin-35 (IL-35) is a newly identified anti-inflammatory cytokine generated by T regulatory cells. They are most effective at high inflammation sites and are a potent activator of T regulatory cells. Hence this study aimed to assess gingival crevicular fluid levels of IL-35 in healthy subjects and chronic periodontitis patients and to compare the levels of IL-35, before and after scaling and root planing in chronic periodontitis patients. Methods: Forty subjects were selected and categorised into 3 groups. Group 1: Periodontally healthy individuals, Group 2A: Chronic periodontitis patients and Group 2B: Group 2A patients who were evaluated 6 weeks after scaling and root planing. Gingival bleeding index, probing pocket depth and clinical attachment level were recorded. Gingival crevicular fluid samples were collected and stored at -800 C till they were subjected to analysis by Enzyme Linked Immunosorbent Assay (ELISA). Results: On intergroup comparison, the difference in mean IL-35 levels and clinical parameters were highly statistically significant between Group 1 and Group 2A while between Group 1 and Group 2B were not significant. On comparing Group 2A and Group 2B, the mean differences were highly statistically significant P (<0.001). Significant reductions in all parameters were noted in Group 2B. Conclusion: Significant reduction of IL-35 levels in chronic periodontitis patients, 6-8 weeks after scaling and root planing and their presence in periodontally healthy subjects suggest the role of IL-35 in controlling the inflammation and their protective role in maintaining periodontal health. Key message: IL-35 has an effective role in controlling the inflammation and in maintaining periodontal health. IL-35 can be considered as a promising prognostic biomarker in periodontal diseases.

scholarly journals A COMPARATIVE EVALUATION OF C-REACTIVE PROTEIN GCF LEVEL IN PERIODONTITIS PATIENTS AFTER SCALING AND ROOT PLANING WITH AND WITHOUT DIODE LASER

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Sangeeta Roy ◽  
C.S. Joshi ◽  
Pradeep Shukla ◽  
Gaurav Malhotra ◽  
Prerna Kataria ◽  
...  
Keyword(s):  
Diode Laser ◽  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Periodontal Therapy ◽  
Type I ◽  
Scaling And Root Planing ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Root Planing ◽  
Crevicular Fluid

Background and Objectives: C?reactive protein (CRP) is a type I acute phase reactant. A number of studies have reported elevated gingival crevicular fluid (GCF) CRP levels in periodontitis subjects, which decrease following periodontal therapy. Effect of diode laser as an adjuct to Scaling & Root planing is also well stablished. The aim of the present study was to evaluate the effect of periodontal treatment SRP with diode laser, on CRP levels in GCF in patients with chronic periodontitis. Materials and Methods: A total of 40 subjects with moderate periodontitis based on community periodontal index scores, were included in the study. Periodontal therapy was performed dividing each side of jaw as a group (Split mouth); one side SRP alone & another Diode laser with SRP. GCF was collected from each subject at Baseline (prior to treatment) and 1 month after periodontal therapy. The collected sample was subjected to biochemical analysis to detect CRP levels by using a commercially available highly sensitive kit. Results: The present study demonstrated that the mean CRP values at baseline were found to be 0.11043mg/l in side-I (side treated with SRP alone); 0.11042mg/l in side-II (side treated with SRP & laser) of the patient, which reduced to 0.4148 mg/L in side I and 0.3985mg/L in side II after treatment, which are highly significant according to statistical analysis but the changes between two sides were non- significant statistically. Interpretation and Conclusion: Within the limitations of this study, it can be concluded that periodontal therapy is able to reduce the GCF C reactive protein level significantly, but there was no statistically significant result in CRP level, between sides treated with SRP & SRP with laser. Key words: Chronic periodontitis, C?reactive protein, gingival crevicular fluid, Scaling and root planing, Diode laser

Efficacy of subgingivally delivered atorvastatin and simvastatin as an adjunct to scaling and root planing

2015 ◽  
Vol 30 (4) ◽  
Author(s):  
Sai M. Surve ◽  
Anirudh B. Acharya ◽  
Srinath L. Thakur
Keyword(s):  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Local Delivery ◽  
Control Group ◽  
Clinical Parameters ◽  
Scaling And Root Planing ◽  
Root Planing ◽  
Significant Difference ◽  
Group 2 ◽  
Crevicular Fluid

AbstractThe current understanding of the pathogenesis of periodontal disease has resulted in adjunctive use of various pharmacologic agents in periodontal therapy. The objective of this investigation was to assess the efficacy of atorvastatin and simvastatin (because of their pleiotropic properties) as an adjunct to dental scaling and root planing (SRP) by local delivery, i.e. placing them subgingivally, in the treatment of chronic periodontitis.Local delivery systems for atorvastatin and simvastatin were prepared in sodium alginate suspension to be administered with calcium chloride solution. Patients diagnosed with chronic periodontitis were grouped as group 1, receiving SRP only (control), group 2, receiving SRP with subgingival delivery of 1.2% simvastatin, and group 3, receiving SRP with subgingival delivery of 1.2% atorvastatin. Clinical parameters and interleukin (IL) 1α levels in the gingival crevicular fluid (GCF) were assessed.All three groups showed significant reductions in clinical parameters and IL-1α levels in the GCF (p<0.05). However, the test groups did not show any statistically significant difference when compared with control.Subgingivally delivered atorvastatin and simvastatin as an adjunct to SRP is efficacious but did not demonstrate any added benefit as compared with SRP alone.

Effect of laser-assisted scaling and root planing on the expression of pro-inflammatory cytokines in the gingival crevicular fluid of patients with chronic periodontitis: A systematic review

2017 ◽  
Vol 18 ◽  
pp. 63-77 ◽  
Author(s):  
Sergio Varela Kellesarian ◽  
Vanessa Ros Malignaggi ◽  
Hasham Abdullah Majoka ◽  
Abdulaziz A. Al-Kheraif ◽  
Tammy Varela Kellesarian ◽  
...  
Keyword(s):  
Systematic Review ◽  
Inflammatory Cytokines ◽  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Scaling And Root Planing ◽  
Root Planing ◽  
Crevicular Fluid ◽  
Pro Inflammatory Cytokines

scholarly journals The effect of carbonate apatite membrane gelatin application on IL-1β level gingival crevicular fluid in chronic periodontitis patients

2019 ◽  
Vol 31 (3) ◽  
pp. 184
Author(s):  
Indra Mustika Setia Pribadi ◽  
Ina Hendiani ◽  
Reynaldy Sartiono
Keyword(s):  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Test Group ◽  
Wilcoxon Test ◽  
Periodontal Pocket ◽  
Control Group ◽  
Carbonate Apatite ◽  
Scaling And Root Planing ◽  
Root Planing ◽  
Crevicular Fluid

Introduction: Apatite carbonate material plays an important role in bone tissue regeneration. The use of this membrane is expected to achieve better treatment success than those without additional therapy. The antimicrobial content in the apatite carbonate membrane can be used to support periodontal treatment of chronic periodontitis after scaling and root planing. The purpose of this research was to analyse the effect of carbonate apatite membrane gelatin application on the IL-1β level of the gingival crevicular fluid in chronic periodontitis patients. Methods: This research was a double-blind, randomised controlled trial method, with purposive sampling, and split-mouth design. The parameter was the IL-1β level in the gingival crevicular fluid before and after scaling and root planing. Carbonate apatite membrane gelatin was applied to the periodontal pocket on the test group after scaling and root planing. Data were analysed by the Wilcoxon test with a p-value < 0.05. Results: The reduction of IL-1β level gingival crevicular fluid on day-0 and day-30 on both groups showed significant value (p < 0.001). However, it showed no significant differences statistically, between the test group and the control group. Conclusion: Carbonate apatite membrane gelatin application might reduce the IL-1β level of the gingival crevicular fluid, but not showing a better reduction from the control group.Keyword: Carbonate apatite membrane gelatin, IL-1β, periodontal therapy

scholarly journals Effect of Diode Laser on Gingival Crevicular Fluid Stromal Derived Factor – 1 Alpha Level in Chronic Periodontitis Patients- A Randomized Clinical Trial

2019 ◽  
Vol 1 (5) ◽  
Author(s):  
Rakesh M.P. ◽  
Krishna Kripal ◽  
Deepthi R. ◽  
Sandeep S. Prabhu ◽  
Shanmugapriya P.A.
Keyword(s):  
Periodontal Disease ◽  
Diode Laser ◽  
Gingival Crevicular Fluid ◽  
Immune Defense ◽  
Clinical Parameters ◽  
Scaling And Root Planing ◽  
Root Planing ◽  
Group 2 ◽  
Crevicular Fluid ◽  
Group 1

Background: SDF-1α may be involved in the immune defense pathway activated during periodontal disease. Upon development of disease, SDF-1α levels increase and may recruit host defensive cells into sites of inflammation. This suggests that SDF-1α may be a useful biomarker for the identification of periodontal disease progression. Previous studies suggested that diode laser using as an adjunct to SRP in clinical and biochemical benefits. Aims and objectives: The aim of the present study was to assess the effect of diode laser on gingival crevicular fluid stromal derived factor – 1 alpha (SDF-1α) level in chronic generalized periodontitis patients.  Materials and methods: A total of 30 patients were divided in two groups based on inclusion and exclusion criteria as follows: Group 1:  15 chronic generalized periodontitis patients who received treatment with scaling and root planing. Group 2: 15 chronic generalized periodontitis patients who received treatment with scaling and root planing and diode laser. Each GCF samples were collected at baseline, 15 days and 21 days after treatment. Clinical parameters like gingival index, probing pocket depth and clinical attachment level were recorded and GCF samples also collected. Results: All the clinical parameters (GI, PPD, CAL) and the concentration of SDF-1α level in GCF were found to be significantly reduced in the group 2 compared to group 1 after 21 days. The mean values of GI, PPD, CAL parameters at different time intervals was found statistically significant in both the groups (p<0.001). Conclusion: Within the limitations of the present study, we conclude that diode laser used as an adjunct to SRP provides greater improvement in clinical and biochemical benefits over conventional mechanical treatment during medium term of observation.

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