scholarly journals RNAi and CRISPR: Promising Tool for Gene Silencing

2021 ◽  
Vol 40 (3) ◽  
Author(s):  
Priyabrata Sen
Keyword(s):  
Gene Silencing ◽  
Promising Tool

scholarly journals Dendrimers as Carriers for siRNA Delivery and Gene Silencing: A Review

2013 ◽  
Vol 2013 ◽  
pp. 1-16 ◽  
Author(s):  
Jiangyu Wu ◽  
Weizhe Huang ◽  
Ziying He
Keyword(s):  
Gene Silencing ◽  
Delivery System ◽  
Sirna Delivery ◽  
Dna Delivery ◽  
Small Interfering Rnas ◽  
Dna And Rna ◽  
Promising Tool ◽  
Knowing That ◽  
Delivery Efficiency ◽  
Sirna Delivery System

RNA interference (RNAi) was first literaturally reported in 1998 and has become rapidly a promising tool for therapeutic applications in gene therapy. In a typical RNAi process, small interfering RNAs (siRNA) are used to specifically downregulate the expression of the targeted gene, known as the term “gene silencing.” One key point for successful gene silencing is to employ a safe and efficient siRNA delivery system. In this context, dendrimers are emerging as potential nonviral vectors to deliver siRNA for RNAi purpose. Dendrimers have attracted intense interest since their emanating research in the 1980s and are extensively studied as efficient DNA delivery vectors in gene transfer applications, due to their unique features based on the well-defined and multivalent structures. Knowing that DNA and RNA possess a similar structure in terms of nucleic acid framework and the electronegative nature, one can also use the excellent DNA delivery properties of dendrimers to develop effective siRNA delivery systems. In this review, the development of dendrimer-based siRNA delivery vectors is summarized, focusing on the vector features (siRNA delivery efficiency, cytotoxicity, etc.) of different types of dendrimers and the related investigations on structure-activity relationship to promote safe and efficient siRNA delivery system.

scholarly journals An artificial cationic oligosaccharide combined with phosphorothioate linkages strongly improves siRNA stability

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Atsushi Irie ◽  
Kazuki Sato ◽  
Rintaro Iwata Hara ◽  
Takeshi Wada ◽  
Futoshi Shibasaki
Keyword(s):  
Gene Silencing ◽  
Rna Binding ◽  
Small Interfering Rnas ◽  
Chemical Modifications ◽  
Promising Tool ◽  
Rna Duplexes ◽  
The Stability ◽  
Multiple Chemical ◽  
Thermal Stability Of

Abstract Small interfering RNAs (siRNAs) are potential tools for gene-silencing therapy, but their instability is one of the obstacles in the development of siRNA-based drugs. To improve siRNA stability, we synthesised a double-stranded RNA-binding cationic oligodiaminogalactose 4mer (ODAGal4) and investigated here its characteristics for siRNA stabilisation in vitro. ODAGal4 improved the resistance of various siRNAs against serum degradation. The effect of ODAGal4 on siRNA stabilisation was further amplified by introduction of modified nucleotides into the siRNA. In particular, a combination of ODAGal4 and incorporation of phosphorothioate linkages into the siRNA prominently prevented degradation by serum. The half-lives of fully phosphorothioate-modified RNA duplexes with ODAGal4 were more than 15 times longer than those of unmodified siRNAs without ODAGal4; this improvement in serum stability was superior to that observed for other chemical modifications. Serum degradation assays of RNAs with multiple chemical modifications showed that ODAGal4 preferentially improves the stability of RNAs with phosphorothioate modification among chemical modifications. Furthermore, melting temperature analysis showed that ODAGal4 greatly increases the thermal stability of phosphorothioate RNAs. Importantly, ODAGal4 did not interrupt gene-silencing activity of all the RNAs tested. Collectively, these findings demonstrate that ODAGal4 is a potent stabiliser of siRNAs, particularly nucleotides with phosphorothioate linkages, representing a promising tool in the development of gene-silencing therapies.

Multiplex gene silencing as a promising tool for development of next generation immune effector cell therapies

Cytotherapy ◽  
2021 ◽  
Vol 23 (5) ◽  
pp. S85
Author(s):  
A. Roussel-Gervais ◽  
A. Sakic ◽  
O. Cherpin ◽  
S. Ilmjärv ◽  
P. Salmon ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Effector Cell ◽  
Immune Effector Cell ◽  
Next Generation ◽  
Cell Therapies ◽  
Immune Effector ◽  
Promising Tool

Practical applications of scanning tunneling microscopy

1989 ◽  
Vol 47 ◽  
pp. 18-19
Author(s):  
D. R. Denley
Keyword(s):  
High Resolution ◽  
Scanning Tunneling Microscopy ◽  
Quantitative Information ◽  
Digital Data ◽  
Scanning Tunneling ◽  
Ambient Atmosphere ◽  
Tunneling Microscopy ◽  
Clear Trend ◽  
Practical Applications ◽  
Promising Tool

Scanning tunneling microscopy (STM) has recently been introduced as a promising tool for analyzing surface atomic structure. We have used STM for its extremely high resolution (especially the direction normal to surfaces) and its ability for imaging in ambient atmosphere. We have examined surfaces of metals, semiconductors, and molecules deposited on these materials to achieve atomic resolution in favorable cases.When the high resolution capability is coupled with digital data acquisition, it is simple to get quantitative information on surface texture. This is illustrated for the measurement of surface roughness of evaporated gold films as a function of deposition temperature and annealing time in Figure 1. These results show a clear trend for which the roughness, as well as the experimental deviance of the roughness is found to be minimal for evaporation at 300°C. It is also possible to contrast different measures of roughness.

968: Gelsolin Gene Silencing Involving Unusual Chromatin Structures and a Novel Stem Loop Structure of the Promoter Region in Human Bladder Cancer

2004 ◽  
Vol 171 (4S) ◽  
pp. 256-257
Author(s):  
Kazunori Haga ◽  
Ataru Sazawa ◽  
Toru Harabayashi ◽  
Nobuo Shinohara ◽  
Minoru Nomoto ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Gene Silencing ◽  
Promoter Region ◽  
Loop Structure ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Stem Loop ◽  
Stem Loop Structure

Cochlear Microphonics: A Promising Tool for Hearing Monitoring in Cerebellopontine Angle Surgery

2014 ◽  
Vol 75 (S 02) ◽  
Author(s):  
Beatriz Madero ◽  
P. Avan ◽  
A. Bazin ◽  
A. Chays ◽  
T. Mom ◽  
...  
Keyword(s):  
Cerebellopontine Angle ◽  
Cochlear Microphonics ◽  
Promising Tool

TRUE Gene Silencing: Unique Gene Silencing by the tRNA Maturase tRNase ZL under the Direction of Small-guide RNA

2007 ◽  
Vol 49 (1) ◽  
pp. 54-64
Author(s):  
Aiko Nakashima ◽  
Masayuki Nashimoto ◽  
Masato Tamura
Keyword(s):  
Gene Silencing ◽  
Guide Rna ◽  
Unique Gene
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