scholarly journals Application of DNA-based Techniques for Intraspecies Differentiation of Hake Fish

2009 ◽  
Vol 78 (4) ◽  
pp. 673-678 ◽  
Author(s):  
Zora Hubálková ◽  
Petr Králík ◽  
Janka Kasalová ◽  
Eva Renčová
Keyword(s):  
Fish Species ◽  
Merluccius Productus ◽  
Rights Protection ◽  
Fish Meat ◽  
Fish Species Identification ◽  
Pcr Rflp ◽  
Species Substitution ◽  
Sea Fish ◽  
Argentine Hake

Fish species identification is important as the interest of consumers in sea-fish meat is increasing. The aim of this study was to determine hake species distribution on the Czech market by the PCR-RFLP and sequencing of mt cyt b, and to develop and optimise an alternative system for determination of hake species by sequencing and/or PCR-RFLP using Pan I sequence. Among 20 samples of hake obtained on the Czech market three species were identified: North Pacific hake (Merluccius productus), Argentine hake (Merluccius hubbsi) and South Pacific hake (Merluccius gayi). The approaches tested in our study represent a significant tool either for the differentiation of hake species from other gadoid species (PCR) or for intraspecies identification of different hake species (PCR-RFLP, sequencing). This knowledge can be applied in detection of fish species substitution within the consumers' rights protection.

Development of a COX 1 based PCR-RFLP method for fish species identification

Food Control ◽  
2015 ◽  
Vol 55 ◽  
pp. 39-42 ◽  
Author(s):  
Steffen Mueller ◽  
Sara M. Handy ◽  
Jonathan R. Deeds ◽  
Gideon O. George ◽  
Wendy J. Broadhead ◽  
...  
Keyword(s):  
Species Identification ◽  
Fish Species ◽  
Fish Species Identification ◽  
Pcr Rflp ◽  
Cox 1

Sea fish fraud? A confirmation of Gadoid species food labelling

2017 ◽  
Vol 119 (1) ◽  
pp. 122-130 ◽  
Author(s):  
Veronika Kyrova ◽  
Pavla Surmanova ◽  
Vladimir Ostry ◽  
Irena Rehurkova ◽  
Jiri Ruprich ◽  
...  
Keyword(s):  
Fish Species ◽  
Gadus Morhua ◽  
Atlantic Cod ◽  
Accurate Identification ◽  
Content Type ◽  
Fish Meat ◽  
Fish Fillets ◽  
Quality Of Food ◽  
Sea Fish

Purpose Gadoid fish and hake are the species of fishes most frequently imported to the Czech Republic. The purpose of this paper, cross-country hygiene study, is to determine sea fish fraud labelling on the Czech market and catering. Design/methodology/approach In total, 57 samples of commercial Gadoid fish product from different manufacturers, distributors and catering facilities were gathered. Gadidae family, hake (Merluccius spp. Raf.), saithe (Pollachius virens L.), Atlantic cod (Gadus morhua L.), Alaska pollock (Theragra chalcogramma Pall.), were detected in fish meat, fish products and fish meals by the qualitative PCR method. Findings In total, 47 samples were labelled as a Gadoid fish, which were confirmed in 43 cases. Six samples were labelled as a hake and were confirmed in five samples. Four samples were labelled as a fish fillets. Three samples of fish fillets were identified as a hake and one sample was detected as a mixture of Alaska pollock and Atlantic cod. Social implications In recent years, due to increasing interest from consumers in the sea fish meat market, accurate Identification of fish species has become more important. The mislabelling of sea fish species, whether intentional or not, was on observed on the Czech market and in catering facilities. Economic factors influence the accuracy of labelling of fish meat, which is a concern as mislabelling can threaten public health. Originality/value The study was concentrated on the monitoring of hygiene and quality of food products and catering facilities. This study provides greater awareness of the condition and quality of food on the market and to the extent of fraudulent practices amongst dealers and producers.

Determination of heavy metals in selected black sea fish species

Food Control ◽  
2017 ◽  
Vol 72 ◽  
pp. 313-318 ◽  
Author(s):  
Lubomir Makedonski ◽  
Katya Peycheva ◽  
Mona Stancheva
Keyword(s):  
Heavy Metals ◽  
Black Sea ◽  
Fish Species ◽  
Sea Fish

scholarly journals Methods of gadoid fish species identification in food and their economic impact in the Czech Republic: a review

2008 ◽  
Vol 52 (No. 7) ◽  
pp. 273-292 ◽  
Author(s):  
Z. Hubalkova ◽  
P. Kralik ◽  
B. Tremlova ◽  
E. Rencova
Keyword(s):  
Czech Republic ◽  
Fish Species ◽  
Dna Analysis ◽  
Immunological Methods ◽  
Fish Products ◽  
The Czech Republic ◽  
Fish Meat ◽  
Discriminating Power ◽  
Heat Treated ◽  
Fish Species Identification

The purpose of the present study was to give an overview of imported and traded gadoid fish species (<i>Gadiformes</i>) in the Czech Republic and to describe available methods for their authentication. Due to the increasing interest of customers in the purchase of buy fish meat and other seafood animals, it is necessary to have available analytical methods with discriminating power of respective fish species. With regard to different values and prices of various fish species, these may be adultered. Until recently, electrophoretic, chromatographic and immunological methods based on the analysis of proteins extracted from fish musculature seemed to be promising. Using these methods, various fish species can be identified in fresh, chilled and frozen products. However, they often fail in heat treated products. Molecular biology methods based on DNA analysis are more reliable and suitable for the analysis of fish products that have been heat treated during the production process.

DETERMINATION OF P53 GENE CODON 72 POLYMORPHISMS IN PATIENTS WITH GASTRIC CANCER

2013 ◽  
pp. 11-17
Author(s):  
Thi Tuy Ha Nguyen ◽  
Thi Minh Thi Ha
Keyword(s):  
Gastric Cancer ◽  
Cancer Patients ◽  
P53 Gene ◽  
Diffuse Gastric Cancer ◽  
Codon 72 ◽  
Cancer Group ◽  
Pcr Rflp ◽  
Gastric Cancer Patients ◽  
The Relationship

Background: The role of p53 gene in the gastric cancer is still controversial. This study is aimed at determining the rate of the p53 gene codon 72 polymorphisms in gastric cancer patients and evaluating the relationship between these polymorphisms and endoscopic and histopathological features of gastric cancer. Patients and methods: Sixty eight patients with gastric cancer (cases) and one hundred and thirty six patients without gastric cancer (controls) were enrolled. p53 gene codon 72 polymorphisms were determined by PCR-RFLP technique with DNA extracted from samples of gastric tissue. Results: In the group of gastric cancer, Arginine/Argnine, Arginine/Proline and Proline/Proline genotypes were found in 29.4%, 42.7% and 27.9%, respectively. The differences of rates were not statistically significant between cases and controls (p > 0,05). In males, the Proline/Proline genotype was found in 38.1% in patients with gastric cancer and more frequent in patients without gastric cancer (15.7%, p = 0,01). An analysis of ROC curve showed that the cut-off was the age of 52 in the Proline/Proline genotype, but it was 65 years old in the Arginine/Proline genotype. The Proline/Proline genotype was found in 41.9% in Borrmann III/IV gastric cancer, this rate was higher than Borrmann I/II gastric cancer (16.2%, p = 0.037) and also higher than controls (18.4%, p = 0,01). The rate of Proline/Proline genotype was 41.7% in the diffuse gastric cancer, it was higher than in controls (p = 0,023). Conclusion: No significative difference of rate was found in genotypes between gastric cancer group and controls. However, there was the relationship between Proline/Proline genotype and gastric cancer in males, Borrmann types of gastric cancer, the diffuse gastric cancer. Key words: polymorphism, codon 72, p53 gene, PCR - RFLP, gastric cancer.

Detection of fish species substitution frauds in Italy: A targeted National Monitoring Plan

Food Control ◽  
2019 ◽  
Vol 101 ◽  
pp. 151-155 ◽  
Author(s):  
Pier Luigi Acutis ◽  
Valentina Cambiotti ◽  
Maria Vittoria Riina ◽  
Serena Meistro ◽  
Cristiana Maurella ◽  
...  
Keyword(s):  
Fish Species ◽  
Monitoring Plan ◽  
National Monitoring ◽  
Species Substitution

scholarly journals The Molecular Bases Study of the Inherited Diseases for the Health Maintenance of the Beef Cattle

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 678
Author(s):  
Elena Konovalova ◽  
Olga Romanenkova ◽  
Olga Kostyunina ◽  
Elena Gladyr
Keyword(s):  
Dna Analysis ◽  
Genetic Defects ◽  
Health Maintenance ◽  
Angus Cattle ◽  
Hereford Cattle ◽  
Pcr Rflp ◽  
Cattle Herds ◽  
Molecular Bases ◽  
Further Development

The article highlighted the problem of meat cattle genetic defects. The aim was the development of DNA tests for some genetic defects diagnostics, the determination of the animal carriers and their frequencies tracking in time. The 1490 DNA samples from the Aberdeen Angus (n = 701), Hereford (n = 385), Simmental (n = 286) and Belgian Blue (n = 118) cattle have been genotyped on the genetic defects by newly created and earlier developed DNA tests based on AS-PCR and PCR-RFLP methods. The Aberdeen Angus cattle genotyping has revealed 2.38 ± 0.31% AMC-cows and 1.67 ± 0.19 % AMC-bulls, 0.65 ± 0.07% DDC-cows and 0.90 ± 0.10% DDC-bulls. The single animals among the Hereford cattle were carriers of MSUD and CWH (on 0.27 ± 0.05%), ICM and HY (on 0.16 ± 0.03%). The Simmental cattle were free from OS. All Belgian Blue livestock were M1- and 0.84%-CMD1-carriers. The different ages Aberdeen Angus cattle genotyping has shown the tendency of the AMC- and DDC frequencies to increase in the later generations. The statistically significant increase of DDC of 1.17% in the cows’ population born in 2019 compared to those born in 2015 allows concluding the further development of the DNA analysis-based measures preventing the manifestation of the genetic anomalies in meat cattle herds is necessary.

Gas Chromatographic Method for Determination of Dimethylamine, Trimethylamine, and Trimethylamine Oxide in Fish-Meat Frankfurters

1991 ◽  
Vol 74 (2) ◽  
pp. 400-403
Author(s):  
Walter Fiddler ◽  
Robert C Doerr ◽  
Robert A Gates
Keyword(s):  
Atlantic Menhaden ◽  
Chromatographic Technique ◽  
Acid Extraction ◽  
Fish Meat ◽  
Flame Ionization ◽  
Trimethylamine Oxide ◽  
Headspace Gas ◽  
Gas Chromatographic Method ◽  
Gas Tight

Abstract A method Is described for analysis of minced fish-meat and surlmi-meat frankfurters for dimethylamine (DMA), trimethyiamine (TMA), and trimethyiamine oxide (TMAO) using a headspace-gas chromatographic technique. After simple acid extraction and addition of NaOH, the headspace was directly Injected Into a gas chromatograph by a gas-tight syringe. DMA and TMA were separated on a Chromosorb 103 column and detected by a flame Ionization detector. TMAO was measured as TMA after Zn reduction. Repeatability of the method for DMA, TMA, and TMAO was 6.6,1.0, and 18.8 ppm, respectively. The method was applicable to Alaska pollock-meat and Atlantic menhaden-meat frankfurters, unwashed, and washed mince and surlml.

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