scholarly journals The Molecular Bases Study of the Inherited Diseases for the Health Maintenance of the Beef Cattle

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 678
Author(s):  
Elena Konovalova ◽  
Olga Romanenkova ◽  
Olga Kostyunina ◽  
Elena Gladyr

The article highlighted the problem of meat cattle genetic defects. The aim was the development of DNA tests for some genetic defects diagnostics, the determination of the animal carriers and their frequencies tracking in time. The 1490 DNA samples from the Aberdeen Angus (n = 701), Hereford (n = 385), Simmental (n = 286) and Belgian Blue (n = 118) cattle have been genotyped on the genetic defects by newly created and earlier developed DNA tests based on AS-PCR and PCR-RFLP methods. The Aberdeen Angus cattle genotyping has revealed 2.38 ± 0.31% AMC-cows and 1.67 ± 0.19 % AMC-bulls, 0.65 ± 0.07% DDC-cows and 0.90 ± 0.10% DDC-bulls. The single animals among the Hereford cattle were carriers of MSUD and CWH (on 0.27 ± 0.05%), ICM and HY (on 0.16 ± 0.03%). The Simmental cattle were free from OS. All Belgian Blue livestock were M1- and 0.84%-CMD1-carriers. The different ages Aberdeen Angus cattle genotyping has shown the tendency of the AMC- and DDC frequencies to increase in the later generations. The statistically significant increase of DDC of 1.17% in the cows’ population born in 2019 compared to those born in 2015 allows concluding the further development of the DNA analysis-based measures preventing the manifestation of the genetic anomalies in meat cattle herds is necessary.

2020 ◽  
Vol 63 (2) ◽  
pp. 409-416
Author(s):  
Elena N. Konovalova ◽  
Olga S. Romanenkova ◽  
Valeria V. Volkova ◽  
Olga V. Kostyunina

Abstract. The use of specialized meat breeds in cattle breeding programs is considered very promising for improving herds' productivity. However, in animal genotype, along with genes that positively affect the productivity signs, there are genes whose mutations, known as genetic defects, negatively affect the health of animals. The aim of the study was the screening of the Russian populations of Aberdeen Angus, Hereford and Belgian Blue cattle on gene mutations associated with the genetic defects of arthrogryposis multiplex (AM), osteopetrosis (OS), developmental duplication (DD), double muscling (M1), hypotrichosis (HY) and maple syrup urine disease (MSUD) as well as the F94L polymorphism of myostatin gene (MSTN) linked with the gene responsible for less fat content in the carcass by means of DNA analysis. In the article, test systems based on the polymerase chain reaction method are presented. The analysis of the Aberdeen Angus (n=4480) population has revealed 0.19 ± 0.09 % animal M1 carriers, 0.53 ± 0.03 % OS carriers, 1.92 ± 0.09 % AM carriers and 9.00 ± 0.20 % DD carriers. The genotyping of Hereford cattle of Russian populations (n=525) has not revealed any individual carriers of MSUD or HY genetic defects. All of the Belgian Blue population (n=92) animals were heterozygous M1 carriers. The study of the F94L MSTN polymorphism has demonstrated extremely high frequencies of the desirable A allele (0.93 and 0.90) in two Aberdeen Angus populations with an average mean of 0.63 ± 0.08, which was 32 % higher compared to the Belgian Blue population. The results suggest the high genetic potential of the Aberdeen Angus and Belgian Blue cattle, but the existence in the genotypes of the mutant alleles associated with hereditary diseases indicates the risk of uncontrolled use of these breeds.


2013 ◽  
pp. 11-17
Author(s):  
Thi Tuy Ha Nguyen ◽  
Thi Minh Thi Ha

Background: The role of p53 gene in the gastric cancer is still controversial. This study is aimed at determining the rate of the p53 gene codon 72 polymorphisms in gastric cancer patients and evaluating the relationship between these polymorphisms and endoscopic and histopathological features of gastric cancer. Patients and methods: Sixty eight patients with gastric cancer (cases) and one hundred and thirty six patients without gastric cancer (controls) were enrolled. p53 gene codon 72 polymorphisms were determined by PCR-RFLP technique with DNA extracted from samples of gastric tissue. Results: In the group of gastric cancer, Arginine/Argnine, Arginine/Proline and Proline/Proline genotypes were found in 29.4%, 42.7% and 27.9%, respectively. The differences of rates were not statistically significant between cases and controls (p > 0,05). In males, the Proline/Proline genotype was found in 38.1% in patients with gastric cancer and more frequent in patients without gastric cancer (15.7%, p = 0,01). An analysis of ROC curve showed that the cut-off was the age of 52 in the Proline/Proline genotype, but it was 65 years old in the Arginine/Proline genotype. The Proline/Proline genotype was found in 41.9% in Borrmann III/IV gastric cancer, this rate was higher than Borrmann I/II gastric cancer (16.2%, p = 0.037) and also higher than controls (18.4%, p = 0,01). The rate of Proline/Proline genotype was 41.7% in the diffuse gastric cancer, it was higher than in controls (p = 0,023). Conclusion: No significative difference of rate was found in genotypes between gastric cancer group and controls. However, there was the relationship between Proline/Proline genotype and gastric cancer in males, Borrmann types of gastric cancer, the diffuse gastric cancer. Key words: polymorphism, codon 72, p53 gene, PCR - RFLP, gastric cancer.


Author(s):  
Т. В. Калінеску ◽  
С. С. Пономарьова

It is examined the influence of the simplified taxation system on stimulation of home agro business development. The aim of the article is research of the existent taxation system of agro producers and optimization of the tax pressure on the payers of the united tax. Methodological basis of research became the modern theories of taxation and finances in the conditions of global calls, climatic risks and necessities of home and foreign consumers. In undertaken studies the used methods of dialectics, methods and principles of scientific cognition and instruments of analysis the systems for the exposure of influence the simplified system of taxation on activity of agro producers. The basic hypothesis of research became supposition that optimization of the tax loading on agro producers must take a place with a selection of volumes of possessing agricultural resources, prepared products and level of the got profits. Exposition of basic material. It is educed that development and total revenue of agro industrial enterprises depend from the level of taxation of their activity, but sum prepaid tax by the agrarian enterprises does not depend from the got profits and does not answer principles of regulative function of taxes. Originality and practical meaningfulness of research are confirmed by the ground analysis of agro producer’s activity, which work for the simplified system of taxation. An proposed differentiation the united tax payers of 4-th group has the applied value, and similar approach can be used for determination of rates the other groups of the simplified system of taxation. Conclusions and prospects of further researches. An offer groupie of agro producers, which work for the simplified system of taxation, will allow bringing down the tax pressure, stimulating further development of agrarian business in the direction of accordance the European standards of agro products quality. Further researches will be sent to the improvement of the simplified system of taxation of stimulation and development of complete cycle of agribusiness beginning from growing of products, processing and, ending, by an eventual consumption is not only food industry, but other industries of national economy, like light industry, green energy and tourism.


Author(s):  
Ekaterina Valer'evna Skalka

The subject of this research is the forecast or prediction of color. The Russian science does not feature work dedicated to such aspect; however, foreign sources approached prediction of color from various perspectives, most often viewing the color trends as an inseparable part of fashion. The goal of this work consists in determination of the place and time of conception of the color forecasts, historical analysis of evolution of this phenomenon, and assumptions on further development of this direction. Territorially, the epicenters of development of color forecasts are determined in Europe (France and England), in America (the United States), in Asia (Japan). The author describes the peculiarities of color predictions in each country – at certain stages one or another county was ahead or behind; whit the advent of the Internet, everything moves to online format and becomes more dynamic. The article follows the forecast of color since its emergence, highlighting all stages of its development and establishment. The chronological framework of its development stages stretches from the early XVIII century until modernity. From the earliest to more recent, these stages include the color cards, mediators and agents (between factories, textile workers and customers, and stores), trade fairs and expositions, online services and websites. It is determined that with the course of time, the methods and instruments for predicting color were being accumulated and used together; the new colors were added, while the already existing did not lose their relevance. Despite the development of technologies, a final word in forecasting color trends belongs to a human, based on experience and intuition.


2020 ◽  
Vol 17 (34) ◽  
pp. 93-111
Author(s):  
Saule Amangeldievna MUSSABEKOVA

Identifying semen stains on clothing and bedding is a crucial component in investigating cases of sexual assault. In some cases, clothing and bedding have already been washed before they were removed and sent for forensic examination. There is insufficient data on best practices for handling traces of semen on clothes after washing. This work aimed to study the possibility of identifying traces of semen on clothes after washing using widely used techniques. To simulate typical physical evidence, donor semen samples were applied to pieces of clothing made from various fabrics. The clothes were washed under multiple conditions (with the help of nonbiological and biological (enzyme-containing) agents, and across numerous washing machines). After washing, the washing stains were characterized by the presence of fluorescence signal, spermatozoa (Koren – Stokis method), the determination of acid phosphatase, prostate-specific antigen and semenogelin, as well as the results of serological research according to the AB0 system and DNA analysis. Clothing analysis using these methods was shown to be effective in experimental conditions. However, the presence of enzymes as detergent components designed to destroy biogenic stains significantly affect the results of the identification of semen stains. It has been established that the full genetic profile can be obtained from semen stains even after washing three times. Different strategies are needed to detect, select and identify semen stains depending on the circumstances of a case. It is recommended to examine clothing and bedding, even if the specimens were previously washed.


Author(s):  
N. Prykaziuk ◽  
A. Tkachenkо

The paper suggests a methodical approach to assessing the life insurance companies’ competitiveness, which is based on a combination of ranking and radar methods. The algorithm for such an assessment is developed, which covers seven progressive stages. The respective calculations revealed the most competitive life insurance companies currently existing in Ukraine. The performed analysis proved the existence of system-forming life insurance companies in Ukraine, as well as groups of institutions that, under certain conditions, can compete significantly or even take a leading position in the market. It is proved that the establishment of competitive positions of such institutions in the relevant market is not only economic but also social in nature, because it allows potential policyholders quickly determining the integrated state of development of a particular insurer. It is substantiated that such calculations are necessary for insurers, as well as for the relevant state authorities, concerning the determination of further development prospects for the cash-value life insurance. Such an analysis is also justified under the changing regulation model of the life insurance companies, as one of the key tasks of the NBU is to strengthen the requirements for their activities, as a result, only the most developed institutions will remain on the market. It is proved that maintaining its place in the market by existing insurers-leaders and, at the same time, gaining new additional prospects for strengthening their market positions by other life insurance companies, requires using a flexible development strategy, which will be based on customer orientation. The ways for improvement of traditional development strategies of life insurance companies are outlined, which allows strengthening their competitive positions in the market under the emerging crisis phenomena in the economic and financial fields.


Author(s):  
Иван Александрович Гринько

Проблема определения социальных функций различных институтов является одной из важнейших для понимания их роли и пути дальнейшего развития. Данная дискуссия отнюдь не носит чисто теоретический характер, наоборот, вопрос о социальном функционале музея является одним из ключевых для их дальнейшего развития. Некоторые исследователи честно признают, что непонимание социальных функций ведет к резкому снижению эффективности работы музеев. В статье анализируются функции музея как социокультурного института на основе такого историко-антропологического источника как анекдот. Эта проблематика, несмотря на свою принципиальную важность, редко анализировалось в антропологическом ключе. Кроме того, взаимосвязь музея и смеховой культуры практически не пользуется вниманием исследователей, хотя данная тема явно имеет большой потенциал. В качестве основного источника для работы был взят массив современных российских анекдотов, к которому для компаративного анализа привлекался массив советских анекдотов. В общей сложности исследовалось более 500 текстов. По итогам исследования можно сделать вывод, что социальные функции музея не ограничиваются традиционными для музеологии вариантами: сохранение наследия, образование и коммуникация. Музей в фольклорных текстах обладает гораздо более широким кругом социальных функций от инструмента символического потребления и валоризации объектов искусства, до пространства эротической игры. Анализ анекдотов, связанных с музеем, показывает, что, несмотря на не самую высокую популярность этого института, в массовой культуре и сознании, в смеховой культуре четко фиксируются его ключевые функции и проблемы. Это еще раз подтверждает важность анализа фольклорных текстов для оценки роли музея в сообществах любого уровня. Данный материал может быть использован для решения самых различных задач музейного менеджмента от оценки эффективности социокультурной деятельности музея до проведения маркетинговых кампаний. The determination of social functions of institute is one of the most difficult issues in social anthropology. This discussion isn’t absolutely theoretical; on the contrary, the issue of the social functionality of the museum is one of the key issues for their further development. Some researchers honestly admit that a lack of understanding of social functions leads to a sharp decline in the effectiveness of museums. The article analyzes the functions of the museum as a sociocultural institution on the basis of such a historical and anthropological source as anecdote. This issue, despite its fundamental importance, has rarely been analyzed in an anthropological vein. In addition, the interconnection between the museum and the culture of laughter has received little attention from researchers, although this topic clearly has great potential. For this research we used block of contemporary Russian anecdotes and the anecdotes of the soviet period (1917–1991) for comparative analysis. Totally more than 500 texts were analyzed. The analysis of the anecdotes associated with the museum shows that, despite the low popularity of this institution, in mass culture and consciousness, in the culture of laughter, its key functions and problems are clearly recorded. This once again confirms the importance of analyzing folklore texts for assessing the role of the museum in communities of any level. This material can be used to solve a variety of problems of museum management, from assessing the effectiveness of the socio-cultural activities of the museum to marketing campaigns.


2018 ◽  
pp. 434-437
Author(s):  
Mohamed Eisa Ismail Dahab

The article by Mohamed Eisa Ismail Dahab, Ambassador Extraordinary and Plenipotentiary of the Republic of the Sudan to Ukraine, is dedicated to the relations between the Sudan and Ukraine. A positive momentum in the bilateral relations of the two countries has been evidenced by the establishment of the Embassy of the Sudan in Ukraine. Since then, both parties have sought to deepen and strengthen cooperation in various fields of mutual interest. The establishment of the Embassy of the Sudan in Kyiv at the ambassadorial level was a crescendo of friendly relations and a display of mutual will and sincere desire to strengthen relations further for the sake of the two nations. The Sudan also provides support to Ukraine on the international arena. Two states defend similar positions in the UN. Ukraine and the Sudan intend to accelerate the speed of bilateral relations and contribute to joint political and economic arrangements, share draft agreements and memoranda of understanding to conclude various contracts on trade and investment, agriculture, education and science, technologies, and other spheres of collaboration for the prosperity and welfare of the two nations. The author puts an emphasis on the importance of economic cooperation. The Sudan imports from Ukraine and spends many billions for various goods and raw materials. Ukraine is a partner in supplying wheat grain and flour, oil and seeds of oil plants, and various commodity goods. The Ambassador of the Sudan expresses his gratitude for strong determination of Ukrainian government to contribute to further development and strengthening of relations between Ukraine and the Sudan in different domains. He hopes the states will do their best for permanent cooperation, coordination and friendship. Keywords: trade, investment, the Sudan, Ukraine, economic arrangements, agriculture, bilateral relations.


Author(s):  
Т. В. Калінеску

The modern stage the development of Ukraine economy is require the reasonable methodological aspects to determination of effectiveness and authenticity the existence of clusters, its the applied value and virtual for the purpose of further aspects of development the theoretical principles of management enterprises in the conditions the modern progress of national economy trends. A research purpose is mark research of practice the using of clusters in modern Ukrainian reality, exposure of its virtual constituents, evaluation the role of clustering for development of theory practices the management of enterprises and its influence an economy on the competitiveness of national economy and maintenance of motion to the European and world society. As an object of research economic activity of enterprises the different industries comes forward economies of Ukraine, which are in the search of new directions of innovative development on the basis of creation of different types of clusters. Methods and principles of scientific cognition of general and special methods and receptions became methodological basis of research that the effective used in the process of ground and the applied value of the use of clustering. Including the methods of systematization and generalization? what are used for the analysis of the existent  aspects of creation the clusters in different industries of activity of enterprises; inductions and deductions, what are use for the ground of the applied value of clustering; between disciplinary analogy, what are use for the evaluation the virtual of application and adaptation of clusters to the operating of modern enterprises conditions. Supposition became the basic hypothesis of research, that modern cluster educations in different industries of Ukraine national economy have more virtual than practical application, and - basic its further development its legislatively-normative determination and use must become in the direction of creation the competitive products. The statement of basic materials. It is well-proven that the applied methods of clustering have likeness with the reliable methods of realization of prognosis, when the variety of the possible states of the economic systems is planned. Common reliable idea about development does not give reliable information a cluster about development of single enterprises in it, that is why conduct each in such cluster associations more casual character, than reliable activity to the cluster, has yet a subject. There is authenticity of that not every subject will be consistently and to move on a scenario which is offered in a cluster association, but will incarnate that which answers its idea about prosperity and necessities. In-process of expose was wrote the mechanisms of presentation the participants of cluster about a utility, satisfaction system of values, which is carried in itself by creation of different clusters. Offered approach consideration about cluster society have originality and practical meaningfulness and allow to send processes creation of clusters on determination of compatible values and necessities for its participants and receipt of public economic effects from it. The conducted research allowed to formulate a conclusion, that clustering have the hidden potentials, that not allow effective develop the economy of enterprises, but take in attention the virtual features of perception the clustering, it follows to pay attention to necessity: creation of legislatively-normative base in relation to functioning of clusters, presence of instruments the state support of activity of clusters on regional and national levels for creation of competitive products; creation of compatible values and requirements in mutual relations in a middle clusters and receipt of the objective applied effects in relation to functioning of clustering.


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