scholarly journals Isolation and identification of Dichelobacter nodosus and Fusobacterium necrophorum using the polymerase chain reaction method in sheep with footrot

2015 ◽  
Vol 84 (2) ◽  
pp. 97-104 ◽  
Author(s):  
Ediz Kagan Ozgen ◽  
Seyda Cengiz ◽  
Mustafa Ulucan ◽  
Zafer Okumus ◽  
Asli Kortel ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Small Ruminants ◽  
Fusobacterium Necrophorum ◽  
Chain Reaction ◽  
Culture Methods ◽  
Isolation And Identification ◽  
Dichelobacter Nodosus ◽  
Specificity And Sensitivity ◽  
Pcr Method ◽  
Polymerase Chain

Footrot is an important infectious disease of small ruminants leading to severe economical losses. The aim of the present study was to determine isolation and identification rates ofDichelobacter nodosusandFusobacterium necrophorumin the culture techniques and reveal the specificity and sensitivity of the culture technique based on the polymerase chain reaction (PCR) method in sheep with footrot. Dry swabs and swabs with Amies medium from 83 sheep were subjected to PCR and culture analyses. In dry swabs, 4 samples were positive forF. necrophorumand all were negative forD. nodosus. Colonies in Eugon and Fusobacterium selective agars from swabs with Amies medium were evaluated. Polymerase chain reaction analysis was conducted on macroscopically and microscopically unidentified samples. The positivity rate was 55.4% forD. nodosusand 69.8% forF. necrophorumin cultures from Fusobacterium selective agars. The positivity rate forD. nodosusin Fusobacterium selective agars was higher than that in Eugon agar. Performing PCR and culture methods increased positivity as compared to performing them alone. In comparison with the PCR method, culturing in Fusobacterium selective agars had moderate sensitivity and low specificity forD. nodosus(71.7 and 28.7%) andF. necrophorum(61.3 and 80.0%), respectively. In conclusion, Fusobacterium selective agar (without antibiotics) for isolation and identification ofD. nodosusis superior to Eugon agar.Fusobacterium necrophorumshould also be considered as a provoking agent for footrot in small ruminants. The PCR method on culture increases elucidation of definitive aetiology.

scholarly journals Detection of Fusobacterium necrophorum and Dichelobacter nodosus from cow footrot in the Heilongjiang Province, China

2019 ◽  
Vol 88 (2) ◽  
pp. 165-168
Author(s):  
Jiaqi Han ◽  
Jing Dong ◽  
Wenhui Yang ◽  
Yunze Xu ◽  
Qiming Xing ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Northeast China ◽  
Pcr Primers ◽  
Fusobacterium Necrophorum ◽  
Heilongjiang Province ◽  
Chain Reaction ◽  
Dichelobacter Nodosus ◽  
Important Concern ◽  
Frequent Finding ◽  
Polymerase Chain

Cow footrot in the Heilongjiang Province, northeast China is a problem resulting in lost production in agriculture. In this study, 200 swab samples from footrot lesions of naturally infected cows with odorous exudative inflammation and keratinous hoof separation at 10 farms were examined in the period from May 2016 to May 2017. Twenty cows from each farm were taken for sampling. The samples were examined for detectingthe presence of Dichelobacter nodosus (D. nodosus) and Fusobacterium necrophorum (F. necrophorum). Such detection was carried out using polymerase chain reaction (PCR). The PCR primers were designed to identify the lktA gene, which encodes a leukotoxin unique to F. necrophorum, and the fimA gene of D. nodosus. Of the 200 samples, 111 (55.5%) revealed the presence of F. necrophorum and 11 (5.5%) exhibited D. nodosus. The frequent finding of F. necrophorum in cow farms of Heilongjiang province, northeast China is noteworthy. The possibility of F. necrophorum and D. nodosus infection should be an important concern when controlling cow footrot in China.

scholarly journals Identification of aflatoxigenic fungi using polymerase chain reaction-based assay

2014 ◽  
pp. 259-269 ◽  
Author(s):  
Vladislava Soso ◽  
Marija Skrinjar ◽  
Nevena Blagojev ◽  
Slavica Veskovic-Moracanin
Keyword(s):  
Polymerase Chain Reaction ◽  
Regulatory Gene ◽  
Control Point ◽  
Complex Mixture ◽  
Target Sequence ◽  
Chain Reaction ◽  
Isolation And Identification ◽  
Close Link ◽  
Critical Control Point ◽  
Polymerase Chain

As the aflatoxins represent a health-risk for humans because of their proven carcinogenicity, food-borne fungi that produce them as secondary metabolites, mainly Aspergillus flavus and Aspergillus parasiticus, have to be isolated and identified. The best argument for identifying problem fungi is that it indicates control points within the food system as part of a hazard analysis critical control point (HACCP) approach. This assumes there is a close link between fungus and toxin. Conventional methods for isolation and identification of fungi are time consuming and require admirably dedicated taxonomists. Hence, it is imperative to develop methodologies that are relatively rapid, highly specific and as an alternative to the existing methods. The polymerase chain reaction (PCR) facilitates the in vitro amplification of the target sequence. The main advantages of PCR is that organisms need not be cultured, at least not for a long time, prior to their detection, target DNA can be detected even in a complex mixture, no radioactive probes are required, it is rapid, sensitive and highly versatile. The gene afl-2 has been isolated and shown to regulate aflatoxin biosynthesis in A. flavus. Also, the PCR reaction was targeted against aflatoxin synthesis regulatory gene (aflR1) since these genes are nearly identical in A. flavus and A. parasiticus in order to indicate the possibility of detection of both the species with the same PCR system (primers/reaction). [Projekat Ministarstva nauke Republike Srbije, br. III46009] <br><br><font color="red"><b> This article has been retracted. Link to the retraction <u><a href="http://dx.doi.org/10.2298/APT1647265E">10.2298/APT1647265E</a><u></b></font>

scholarly journals COVID-19, A New Outlook on Pandemic Symptoms and Diagnostic Approach

2022 ◽  
Vol 02 (01) ◽  
Author(s):  
María Fernanda Calderón Hernádez ◽  
Keyword(s):  
Diabetes Mellitus ◽  
Risk Factors ◽  
Polymerase Chain Reaction ◽  
Latin America ◽  
Diagnostic Methods ◽  
Chain Reaction ◽  
Specificity And Sensitivity ◽  
Polymerase Chain ◽  
Effective Diagnosis ◽  
Age And Sex

Background: The main objective of this research is to learn the symptoms that occur in this pathology, since we are currently still fighting COVID-19, because of this, it is important to keep us informed about the different diagnostic methods available, which help us reach an earlier and more effective diagnosis. Various articles have been compiled to identify as soon as possible the active cases and thus reduce the number of infections. Materials and methods: This research was conducted on the basis of scientific articles and books, related to COVID-19. Methods: This research was conducted based on 15 scientific articles and 3 books, related to COVID-19. Results: The most important risk factors are diabetes mellitus, hypertension, obesity, age and sex. The most common symptoms in Latin America are dry cough, fatigue, sore throat, and fever. The preferred diagnostic test for COVID-19 is the polymerase chain reaction for its specificity and sensitivity Conclusions: As a conclusion, the main objective of the research was achieved, which is to inform the reader about the most relevant symptoms of SARS-CoV-2 in order to improve the identification of suspected cases. Furthermore, we compare various diagnostic methods that exist to date and determine that PCR is the most specific and sensitive.

scholarly journals Comparison of polymerase chain reaction and microscopy for the detection of Fasciola spp. in the fecal matter of domestic bovines in Kalasin Province, Thailand

2021 ◽  
pp. 2878-2882
Author(s):  
Sirikanda Thanasuwan ◽  
Anupong Tankrathok
Keyword(s):  
Polymerase Chain Reaction ◽  
False Negative ◽  
Economic Losses ◽  
Rrna Gene ◽  
28S Rrna ◽  
Chain Reaction ◽  
Fecal Matter ◽  
Specificity And Sensitivity ◽  
Polymerase Chain ◽  
Fasciola Spp

Background and Aim: Fasciola spp. are important foodborne trematodes and waterborne zoonotic parasites that cause health problems and economic losses worldwide, including in Thailand. Fasciola spp. are usually detected by sedimentation or the formalin-ethyl acetate concentration technique (FECT) under microscopy, which is less specific and sensitive. Accurate detection is important to detect real incidence for protection against and elimination of fasciolosis in the area. This study aimed to determine the distribution of Fasciola spp. and compare the specificity and sensitivity of FECT under microscopy to that of polymerase chain reaction (PCR) in cattle feces. Materials and Methods: The study was conducted in Kalasin Province, Thailand. Feces of 46 cattle were investigated for infection with Fasciola spp. To detect infection, FECT under microscopy and PCR amplification of the 28S rRNA gene of Fasciola spp. were used to identify egg parasites. Results: Feces of 16 of 46 (34.78%) cattle were positive for Fasciola spp. using FECT under microscopy, whereas PCR showed that 67.39% (31 of 46) were positive for Fasciola spp. False-negative results were as high as 32.61% when diagnosed under microscopy. Conclusion: This study confirmed the infection of cattle with Fasciola spp. in Kalasin Province, indicating that PCR demonstrated higher sensitivity and specificity when diagnosing infection. FECT under microscopy can still be used as a primary and traditional method for diagnosis. However, relapse cases of Fasciola spp. and Paramphistomum spp. should be diagnosed by microscopy combined with PCR. This is the first report on the molecular distribution of fecal samples in cattle in Kalasin Province.

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