Various problems such as sand, mud and water bursting, unstability of tunnel face, large deformation of surrounding rock and severe cracking of primary support occurred in excavation of the water-bearing weak sandstone strata of Huma Ling Tunnel, Lan-Yu railway. Based on the uniaxial compressive strength tests and uniaxial compressive rheological tests on weak sandstone with water content of 0, 2%, 5%, 8% and 12%, the relationship between rheological characteristic and water content of water-bearing weak sandstone is obtained. According to results of tests, factors and water contents are fitted by applying generalized rheological equation of Kelvin. And the rheological equation of water-bearing weak sandstone with various water content is deduced. The test results and conclusion can offer reference for analyzing deformation characteristics and instability mechanism of water-bearing weak sandstone in large cross-section tunnelling.