scholarly journals Effective arbuscular mycorrhiza development between Medicago lupulina and Rhizophagus irregularis under conditions of different phosphorus levels in the substrate

2020 ◽  
Author(s):  
A. P. Yurkov ◽  
A. A. Kryukov ◽  
A. O. Gorbunova ◽  
Yu. V. Mikhaylova ◽  
K. S. Dobryakova ◽  
...  
Keyword(s):  
Arbuscular Mycorrhiza ◽  
Carbohydrate Metabolism ◽  
Phosphate Transport ◽  
Rhizophagus Irregularis ◽  
Transcript Levels ◽  
Medicago Lupulina ◽  
Carbohydrate Metabolism Genes ◽  
Phosphorus Levels

The expressions of phosphate transport and carbohydrate metabolism genes in Medicago lupulina with and without Rhizophagus irregularis inoculation were evaluated. Differences in their transcript levels were found under conditions of different phosphorus levels in substrate.

scholarly journals Carbohydrate metabolism genes and pathways in insects: insights from the honey bee genome

2006 ◽  
Vol 15 (5) ◽  
pp. 563-576 ◽  
Author(s):  
T. Kunieda ◽  
T. Fujiyuki ◽  
R. Kucharski ◽  
S. Foret ◽  
S. A. Ament ◽  
...  
Keyword(s):  
Carbohydrate Metabolism ◽  
Honey Bee ◽  
Carbohydrate Metabolism Genes

scholarly journals Palmitvaccenic Acid (Δ11-cis-hexadecenoic acid) Is Synthesized by an OLE1-like Desaturase in the Arbuscular Mycorrhiza Fungus Rhizophagus irregularis

Biochemistry ◽  
2020 ◽  
Vol 59 (11) ◽  
pp. 1163-1172 ◽  
Author(s):  
Mathias Brands ◽  
Edgar B. Cahoon ◽  
Peter Dörmann
Keyword(s):  
Arbuscular Mycorrhiza ◽  
Rhizophagus Irregularis ◽  
Hexadecenoic Acid

scholarly journals AM-Induced Alteration in the Expression of Genes, Encoding Phosphorus Transporters and Enzymes of Carbohydrate Metabolism in Medicago lupulina

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 486
Author(s):  
Andrey Yurkov ◽  
Alexey Kryukov ◽  
Anastasia Gorbunova ◽  
Andrey Sherbakov ◽  
Ksenia Dobryakova ◽  
...  
Keyword(s):  
Carbohydrate Metabolism ◽  
Expression Profiles ◽  
Phosphorus Deficiency ◽  
Metabolic Enzymes ◽  
Axillary Shoot ◽  
Shoot Branching ◽  
Medicago Lupulina ◽  
Flowering Initiation ◽  
Initiation Stage ◽  
Enzymes Of Carbohydrate Metabolism

Plant–microbe interactions, including those of arbuscular mycorrhiza (AM), have been investigated for a wide spectrum of model plants. The present study focuses on an analysis of gene expression that encodes phosphate and sugar transporters and carbohydrate metabolic enzymes in a new model plant, the highly mycotrophic Medicago lupulina MLS-1 line under conditions of phosphorus deficiency and inoculation with Rhizophagus irregularis. Expression profiles were detected by RT-PCR at six plant stages of development (second leaf, third leaf, shooting, axillary shoot branching initiation, axillary shoot branching, flowering initiation). In comparison to control (without AM), the variant with AM inoculation exhibited a significant elevation of transcription levels of carbohydrate metabolic enzymes (MlSUS, MlHXK1) and sucrose transporters (MlSUC4) in M. lupulina leaves at the shooting stage. We suggest that this leads to a significant increase in the frequency of AM infection, an abundance of mycelium in roots and an increase in AM efficiency (which is calculated by the fresh weight of aerial parts and roots at the axillary shoot branching initiation stage). In roots, the specificity of MlPT4 and MlATP1 gene expressions were revealed for effective AM symbiosis. The level of MlPT4 transcripts in AM roots increased more than tenfold in comparison to that of non-specific MlPT1 and MlPT2. For the first time, MlPT1 expression was shown to increase sharply against MlPT2 in M. lupulina roots without AM at the shooting initiation stage. A significant increase in MlRUB expression was revealed at late stages in the host plant’s development, during axillary shoot branching and flowering initiation. The opposite changes characterized MlHXK1 expression. Alteration in MlHXK1 gene transcription was the same, but was more pronounced in roots. The obtained results indicate the importance of genes that encode phosphate transporters and the enzymes of carbohydrate metabolism for effective AM development at the shooting stage in the host plant.

scholarly journals The effect of inoculation with arbuscular mycorrhizal fungus Rhizophagus irregularis on cytokinin content in a highly mycotrophic Medicago lupulina line under low phosphorus level in the soil

2017 ◽  
Vol 63 (No. 11) ◽  
pp. 519-524 ◽  
Author(s):  
Yurkov Andrey ◽  
Veselova Svetlana ◽  
Jacobi Lidia ◽  
Stepanova Galina ◽  
Yemelyanov Vladislav ◽  
...  
Keyword(s):  
Phosphorus Deficiency ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Zeatin Riboside ◽  
Rhizophagus Irregularis ◽  
Medicago Lupulina ◽  
Hormone Balance ◽  
Low Phosphorus ◽  
Am Symbiosis

The study is focused on the elucidation of the role of cytokinins (CKs, zeatin and zeatin riboside) in the development of effective arbuscular mycorrhiza (AM) symbiosis with Medicago lupulina. An important mechanism involved in the regulation of host plant growth is supposed to be linked to the modulation of plant hormone balance. The data obtained revealed the formation of an effective AM-symbiosis (M. lupulina + Rhizophagus irregularis) under phosphorus-deficiency. At the shooting stage (35<sup>th</sup> day after sowing), it is characterized by a decrease in the root:shoot ratio, the lowering in arbuscules and vesicle abundances, but an increase in the intensity of mycelium development. Mycorrhized plants differed from the control ones by higher CK levels in both roots and leaves. Zeatin and zeatin riboside concentration exhibited uneven alterations over time. A role of mycelium in the modulation of CK balance has been discussed.

scholarly journals Medicago lupulina lines with defects in the development of efficient arbuscular mycorrhiza

2018 ◽  
Vol 16 (4) ◽  
pp. 61-74
Author(s):  
Andrey P. Yurkov ◽  
Lidija M. Jacobi
Keyword(s):  
Arbuscular Mycorrhiza ◽  
Population Analysis ◽  
Molecular Genetic ◽  
Genetic Studies ◽  
Plant Model ◽  
Medicago Lupulina ◽  
Am Symbiosis ◽  
Appressoria Formation ◽  
Low Activity ◽  
Convenient Model

Background. The work is aimed to solve actual problems in biology of arbuscular mycorrhiza (AM). Currently, a lot of mutants had been obtained in various plant model objects with defects in genes controlling AM development, however, the mechanisms controlling development of effective AM symbiosis are still unclear. Materials and methods. The authors conducted a mutagenesis in Medicago lupulina, a new convenient model plant for molecular-genetic studies. High mycotrophic M. lupulina line have early and high response to mycorrhization, high seed production, as well as signs of dwarfism under conditions without of AM and low level of phosphorus available for plants. This method allows visually to identify plant lines with defects in AM symbiosis. Results. 14 modes for mutagenesis by ethylmethanesulfonate were conducted. Usage of 3 mutagenesis modes allowed to obtain: productive M1 progeny with high part of viable seedlings (73.3%–86.0%); 1405 plants in M2 progeny. Conclusion. According to population analysis for mutant plants in M2 progeny (up to M9 generation) 15 plant lines were selected: one Myc– plant line unable to form AM symbiosis, 4 Pen– plant lines unable to form AM symbiosis, but characterized by appressoria formation; 3 Rmd– plant lines forming low-activity ineffective AM symbiosis; 3 Rmd– plant lines forming low-activity effective AM and 4 Rmd++ plant lines forming effective AM with high abundance of symbiotic structures (mycelium/arbuscules/vesicles) in the roots.

scholarly journals Identification of Carbohydrate Metabolism Genes in the Metagenome of a Marine Biofilm Community Shown to Be Dominated by Gammaproteobacteria and Bacteroidetes

Genes ◽  
2010 ◽  
Vol 1 (3) ◽  
pp. 371-384 ◽  
Author(s):  
Jennifer L. Edwards ◽  
Darren L. Smith ◽  
John Connolly ◽  
James E. McDonald ◽  
Michael J. Cox ◽  
...  
Keyword(s):  
Carbohydrate Metabolism ◽  
Marine Biofilm ◽  
Carbohydrate Metabolism Genes ◽  
Biofilm Community

scholarly journals Differential Expression of Lipid and Carbohydrate Metabolism Genes in Upper Airway versus Diaphragm Muscle

SLEEP ◽  
2010 ◽  
Vol 33 (3) ◽  
pp. 363-370 ◽  
Author(s):  
Erik van Lunteren ◽  
Sarah Spiegler ◽  
Michelle Moyer
Keyword(s):  
Carbohydrate Metabolism ◽  
Differential Expression ◽  
Upper Airway ◽  
Diaphragm Muscle ◽  
Carbohydrate Metabolism Genes
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