scholarly journals Development of Lactobacillus kimchicus DCY51T Mediated Gold Nanoparticles for Delivery of Ginsenoside Compound K with Photoluminescence and Internal Reflection Scattering Towards Cancer Cell Lines

2018 ◽  
Vol 2 (3) ◽  
2009 ◽  
Vol 43 (12) ◽  
pp. 1214-1224 ◽  
Author(s):  
Shinji Ito ◽  
Norio Miyoshi ◽  
William G. Degraff ◽  
Kunio Nagashima ◽  
Louis J. Kirschenbaum ◽  
...  

2019 ◽  
Vol 58 ◽  
pp. 1-9 ◽  
Author(s):  
Mehdi Tayybi Azar ◽  
Necdet Saglam ◽  
Mustafa Turk

Wnt-β Catenin pathway has an important role in many cancers. Wnt-1 protein from the Wnt protein family, which regulates this pathway, has a special effect on the development of breast cancer. Monoclonal antibodies attaching to metal nanoparticles have an important role in the diagnosis and treatment of cancers.In this study, the anti-Wnt-1monoclonal antibody was conjugated to the gold nanoparticles synthesized by theTurkevichmethod. Conjugation was achieved using the EDC-NHS method. The density of the monoclonal antibodies bonded to gold nanoparticles was measured by Roche Cobas Integra 400 Plus device. MCF-7 and MDA-MB-231 breast cancer cell lines were treated with conjugated nanoparticles for 48 h, followed by performing a double-staining method to detect apoptosis cells.The results showed that inhibition of Wnt-1 protein in extracellular matrix causes apoptosis and gold nanoparticles to have a positive effect on Anti-Wnt-1monoclonal antibodies, leading to an increase in the number of conjugating nanoparticles.


Author(s):  
Pannerselvam Balashanmugam ◽  
Prabhu Durai ◽  
Manickam Dakshinamoorthi Balakumaran ◽  
Pudupalayam Thangavelu Kalaichelvan

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