Co-Incidence of Human Papillomaviruses and Epstein–Barr Virus is Associated with Advanced Tumor Stage: A Tissue Microarray Study of Head and Neck Cancers

Abstract Background High-risk human papillomaviruses (HPVs) are present and can cooperate with Epstein–Barr virus (EBV) to initiate and/or enhance the progression of several types of human carcinomas including cervical as well as head and neck; in parallel, it has been recently pointed out that these oncoviruses can be detected in human breast cancers. Thus, we herein explored the presence/co-presence of high-risk HPVs and EBV in breast cancer in Lebanese women. Methods A cohort of 102 breast cancer samples and 14 normal breast tissues were assessed for the presence of HPVs and EBV. Polymerase chain reaction (PCR) and immunohistochemistry (IHC) analysis in addition to tissue microarray (TMA) platform were used in this study. Results We found the presence of HPV in 66/102 (65%) of our samples, while EBV is present in 41/102 (40%) of the cohort. Additionally, our data showed that high-risk HPV types (52, 35, 58, 45, 16 and 51) are the most frequent in breast cancer in Lebanese women. Meanwhile, we report that high-risk HPVs and EBV are co-present in 30/102 (29%) of the samples; more significantly, our results indicate that their co-presence is associated with tumor grade (p = 0.03). Conclusion Our data revealed that HPVs and EBV are present/co-present in human breast cancer where they may play an important role in its development and/or progression; thus, we believe that further investigations are essential to confirm and elucidate the presence/co-presence of these oncoviruses and the underlying mechanisms of their interaction in breast carcinogenesis.

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Clinicopathological Characteristics and Response to Chemotherapy in Treatment-Naive Epstein–Barr Virus Associated Gastric Cancer: A Retrospective Study

Frontiers in Oncology ◽

10.3389/fonc.2021.611676 ◽

2021 ◽

Vol 11 ◽

Author(s):

Tong Xie ◽

Zhi Peng ◽

Yiqiang Liu ◽

Zhening Zhang ◽

Xiaotian Zhang ◽

...

Keyword(s):

Gastric Cancer ◽

Neoadjuvant Chemotherapy ◽

Vascular Invasion ◽

Epstein Barr Virus ◽

Tumor Stage ◽

Clinicopathological Characteristics ◽

Advanced Tumor Stage ◽

Barr Virus ◽

Response To Chemotherapy ◽

Epstein Barr

BackgroundEpstein–Barr virus associated gastric cancer (EBVaGC) is a special subtype of gastric cancer. However, the perioperative treatment plan and the response to chemotherapy are still uncertain.MethodsWe retrospectively enrolled patients diagnosed with EBVaGC from March 2013 to July 2020 in Beijing Cancer Hospital. Clinicopathological characteristics were recorded. Disease-free survival (DFS) were then calculated, and variants affecting DFS were tested in a Cox proportional regression model.ResultsOne hundred sixty consecutive patients were finally included in our study. Of the patients, 96.9% had adenocarcinoma, while five had squamous cell carcinoma component. Most (70.9%) of them were poorly differentiated. Prevalent programmed death-ligand 1 (PD-L1) (69%) and minor HER-2 (3.8%) expression were noticed; all of the patients were MMR proficient (pMMR) or microsatellite stable (MSS). Among 33 patients who experienced neoadjuvant therapy, the number of tumor regression grade (TRG) 1, TRG 2, and TRG 3 was 5, 16, and 12, respectively. Patients with advanced tumor stage and T stage showed poorer response. Thirty-one patients experienced first-line chemotherapy; ORR was 33.3%, and DCR was 61.9%. One hundred forty-seven patients underwent surgery, and 27 of them showed disease recurrence; the 3-year DFS rate was 71.0%. Tumor stage, neoadjuvant chemotherapy, vascular invasion, and negative PD-L1 expression were associated with poorer DFS. Vascular invasion was the independent risk factor of DFS. Only seven patients reached OS with median follow-up time of 14 months.ConclusionEBVaGC exhibits unique clinicopathological characteristics. Neoadjuvant chemotherapy may not be suitable for EBVaGC, and EBVaGC exhibited relatively poor response to chemotherapy.

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Incidence of Epstein–Barr virus in Syrian women with breast cancer: A tissue microarray study

Human Vaccines & Immunotherapeutics ◽

10.1080/21645515.2015.1009342 ◽

2015 ◽

Vol 11 (4) ◽

pp. 951-955 ◽

Cited By ~ 14

Author(s):

Tahar Aboulkassim ◽

Amber Yasmeen ◽

Nizar Akil ◽

Gerald Batist ◽

Ala-Eddin Al Moustafa

Keyword(s):

Breast Cancer ◽

Tissue Microarray ◽

Epstein Barr Virus ◽

Barr Virus ◽

Microarray Study ◽

Epstein Barr

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High-Risk Human Papillomavirus and Epstein–Barr Virus Coinfection: A Potential Role in Head and Neck Carcinogenesis

Biology ◽

10.3390/biology10121232 ◽

2021 ◽

Vol 10 (12) ◽

pp. 1232

Author(s):

Rancés Blanco ◽

Diego Carrillo-Beltrán ◽

Alejandro H. Corvalán ◽

Francisco Aguayo

Keyword(s):

High Risk ◽

Head And Neck ◽

Tobacco Smoke ◽

Epstein Barr Virus ◽

Potential Role ◽

Human Papillomaviruses ◽

Oral Epithelium ◽

Oncogenic Viruses ◽

Barr Virus ◽

Epstein Barr

High-risk human papillomaviruses (HR-HPVs) and Epstein–Barr virus (EBV) are recognized oncogenic viruses involved in the development of a subset of head and neck cancers (HNCs). HR-HPVs are etiologically associated with a subset of oropharyngeal carcinomas (OPCs), whereas EBV is a recognized etiological agent of undifferentiated nasopharyngeal carcinomas (NPCs). In this review, we address epidemiological and mechanistic evidence regarding a potential cooperation between HR-HPV and EBV for HNC development. Considering that: (1) both HR-HPV and EBV infections require cofactors for carcinogenesis; and (2) both oropharyngeal and oral epithelium can be directly exposed to carcinogens, such as alcohol or tobacco smoke, we hypothesize possible interaction mechanisms. The epidemiological and experimental evidence suggests that HR-HPV/EBV cooperation for developing a subset of HNCs is plausible and warrants further investigation.

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Immunohistochemical detection of human cytomegalovirus, Epstein-Barr virus and human papillomavirus in invasive breast carcinoma in Egyptian women: A tissue microarray study

Journal of Solid Tumors ◽

10.5430/jst.v6n2p8 ◽

2016 ◽

Vol 6 (2) ◽

Cited By ~ 2

Author(s):

Rehab Allah Ahmed ◽

Shaimaa M. Yussif

Keyword(s):

Human Papillomavirus ◽

Breast Carcinoma ◽

Tissue Microarray ◽

Human Cytomegalovirus ◽

Epstein Barr Virus ◽

Immunohistochemical Detection ◽

Barr Virus ◽

Microarray Study ◽

Egyptian Women ◽

Epstein Barr

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Epstein–Barr virus and its association with Fascin expression in colorectal cancers in the Syrian population: A tissue microarray study

Human Vaccines & Immunotherapeutics ◽

10.1080/21645515.2017.1302046 ◽

2017 ◽

Vol 13 (7) ◽

pp. 1573-1578 ◽

Cited By ~ 8

Author(s):

Noor Al-Antary ◽

Hanan Farghaly ◽

Tahar Aboulkassim ◽

Amber Yasmeen ◽

Nizar Akil ◽

...

Keyword(s):

Tissue Microarray ◽

Epstein Barr Virus ◽

Colorectal Cancers ◽

Barr Virus ◽

Microarray Study ◽

Syrian Population ◽

Epstein Barr

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Copresence of High-Risk Human Papillomaviruses and Epstein–Barr Virus in Colorectal Cancer: A Tissue Microarray and Molecular Study from Lebanon

International Journal of Molecular Sciences ◽

10.3390/ijms22158118 ◽

2021 ◽

Vol 22 (15) ◽

pp. 8118

Author(s):

Karim Nagi ◽

Ishita Gupta ◽

Nawaf Jurdi ◽

Amber Yasmeen ◽

Semir Vranic ◽

...

Keyword(s):

Colorectal Cancer ◽

High Risk ◽

Tissue Microarray ◽

Epstein Barr Virus ◽

Molecular Study ◽

Human Papillomaviruses ◽

Colorectal Carcinogenesis ◽

Tissue Samples ◽

Barr Virus ◽

Epstein Barr

Colorectal cancer (CRC) is the third most common cause of cancer-related deaths worldwide. Human papillomaviruses (HPVs) and Epstein–Barr virus (EBV) have been reported to be present in different types of human cancers, including CRCs, where they can play a key role in the onset and/or progression of these cancers. Thus, we herein explored the prevalence of high-risk HPVs and EBV in a cohort of 94 CRC tissue samples and 13 colorectal normal tissues from the Lebanese population using polymerase chain reaction, immunohistochemistry, and tissue microarray methodologies. We found that high-risk HPVs are present in 64%, while EBV is present in 29% of our CRC samples. Additionally, our data showed that high-risk HPV types (16, 18, 35, 58, 51, 45, 52, 31, and 33) are the most frequent in CRC in the Lebanese cohort, respectively. Our data point out that HPVs and EBV are copresent in 28% of the samples. Thus, this study clearly suggests that high-risk HPVs and EBV are present/copresent in CRCs, where they could play an important role in colorectal carcinogenesis. Nevertheless, further investigations using a larger cohort are needed to elucidate the possible cooperation between these oncoviruses in the development of CRC.

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Co-incidence of Human Papillomaviruses and Epstein–Barr Virus Is Associated With High to Intermediate Tumor Grade in Human Head and Neck Cancer in Syria

Frontiers in Oncology ◽

10.3389/fonc.2020.01016 ◽

2020 ◽

Vol 10 ◽

Cited By ~ 1

Author(s):

Ishita Gupta ◽

Lina Ghabreau ◽

Hamda Al-Thawadi ◽

Amber Yasmeen ◽

Semir Vranic ◽

...

Keyword(s):

Head And Neck Cancer ◽

Head And Neck ◽

Neck Cancer ◽

Epstein Barr Virus ◽

Human Head ◽

Tumor Grade ◽

Human Papillomaviruses ◽

Barr Virus ◽

Epstein Barr

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Comparison of Co-Presence of Epstein-Barr Virus and High-Risk Human Papillomaviruses in Colorectal Cancers in the Middle East Region

University of the Future: Re-Imagining Research and Higher Education ◽

10.29117/quarfe.2020.0121 ◽

2020 ◽

Author(s):

Karim Nagi ◽

Ishita Gupta ◽

Hamda A Al-Thawadi ◽

Ayesha Jabeen ◽

Mohammed I. Malk ◽

...

Keyword(s):

Colorectal Cancer ◽

High Risk ◽

Epstein Barr Virus ◽

Human Papillomaviruses ◽

Colorectal Cancers ◽

Barr Virus ◽

Human Carcinomas ◽

Epstein Barr ◽

Invasive Carcinomas ◽

High Risk Hpv

Background: Several studies have shown the presence of onco viral DNA in colorectal tumor tissues. Viral infection by onco-viruses such as Human papillomaviruses (HPVs) and Epstein–Barr virus (EBV) are well-known to be involved in the onset and/or progression of numerous human carcinomas. Methods: We explored the co-presence of high-risk HPVs and EBV in a cohort of colorectal cancer samples from Lebanon (94) and Syria (102) by PCR, immunohistochemistry and tissue microarray. Results: The results of the study point out that 54% of colorectal cancer cases in Syria are positive for high-risk HPVs, while 30% of the cases in Lebanon are positive for these viruses; the most frequent high-risk HPV types in these populations are 16, 18, 31, 33 and 35. Analysis of LMP1 showed similar results in both populations; 36% of Syrian and 31% of Lebanese samples. Additionally, we report that EBV and high-risk HPVs are co-present in these samples. In Syrian samples, EBV and HPVs are co-present in 16% of the population, however, in the Lebanese samples, 20% of the cases are positive for both EBV and HPVs; their co-presence is associated with high/intermediate grade invasive carcinomas. Conclusion: These data suggest that EBV and high-risk HPVs are co-present in human colorectal cancers where they can cooperate in the progression of these cancers. Nevertheless, further studies are needed to elucidate the role of those oncoviruses in the development of human colorectal carcinomas.

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