scholarly journals OPTIMASI KONDISI EKTRAKSI VANILLIN HASIL DEGRADASI LIGNOSELULOSA BAGAS TEBU MENGGUNAKAN RESPONSE SURFACE METHOD (RSM)

2021 ◽  
Vol 8 (1) ◽  
pp. 89-104
Author(s):  
Irnia Nurika ◽  
Faudina Nurin Nisa' ◽  
Nurul Azizah ◽  
Sri Suhartini
Keyword(s):  
Ethyl Acetate ◽  
Response Surface ◽  
Phanerochaete Chrysosporium ◽  
Response Surface Method ◽  
Sugarcane Bagasse ◽  
Extraction Time ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignocellulose Degradation ◽  
Surface Method

Optimization of Vanillin Extraction Conditions from Lignocellulose Degradation of Sugarcane Bagasse using the Response Surface Method (RSM) Sugarcane bagasse is an agricultural waste containing lignocellulose and has the potential to be processed into high-value chemicals such as vanillin. The degradation of sugarcane bagasse lignocellulose can be carried out biologically by the white rot fungus Phanerochaete chrysosporium. This study aims to obtain optimal extraction conditions in the form of ethyl acetate solvent volume and extraction time, using the response surface method (RSM). Two optimized factors were the volume of ethyl acetate (71.72; 80; 100; 120; and 128.28 mL) and the extraction time (35.16; 60; 120; 180; 204.84 minutes). The response variables were the concentration (%) and yield of vanillin (µg g–1). The research on the optimization of the response of vanillin levels and vanillin yield was carried out at 14 days incubation with the highest average total soluble phenol (TSP) value of 0.101 mg g–1. The optimal condition of ethyl acetate volume of 109.730 mL with an extraction time of 137.302 minutes was predicted to produce vanillin levels and yields of 0.0078% and 8.9089 g g–1, respectively, with an accuracy value of 93.4%. Based on the verification results, the optimal vanillin concentration and yield were 0.0077% and 8.9805 g g–1, respectively. Bagas tebu merupakan limbah pertanian yang mengandung lignoselulosa dan berpotensi diolah menjadi bahan kimia bernilai tinggi seperti vanillin. Degradasi lignoselulosa bagas tebu dapat dilakukan secara biologis oleh jamur pelapuk putih Phanerochaete chrysosporium. Penelitian ini bertujuan mendapatkan kondisi ekstraksi optimal berupa volume pelarut etil asetat dan lama waktu ekstraksi, menggunakan response surface method (RSM). Dua faktor yang dioptimasi adalah volume etil asetat (71,72; 80; 100; 120; dan 128,28 mL) dan lama waktu ekstraksi (35,16; 60; 120; 180; 204,84 menit). Variabel respons adalah kadar (%) dan yield vanillin (µg g–1). Penelitian optimasi respons kadar vanillin dan yield vanillin dilakukan pada inkubasi 14 hari dengan nilai total soluble phenol (TSP) rata-rata tertinggi 0,101 mg g–1. Kondisi optimal volume etil asetat 109,730 mL dengan waktu ekstraksi 137,302 menit diprediksi menghasilkan kadar dan yield vanillin sebesar 0,0078% dan 8,9089 µg g–1 dengan nilai ketepatan 93,4%. Berdasar hasil verifikasi, konsentrasi dan yield vanillin yang optimal masing-masing adalah 0,0077% dan 8,9805 µg g–1.

Removal of humic acid from composted hog waste by the white-rot fungus, Phanerochaete chrysosporium

2015 ◽  
Vol 72 (1) ◽  
pp. 92-98 ◽  
Author(s):  
Junying Liu ◽  
Yunmeng Song ◽  
Roger Ruan ◽  
Yuhuan Liu
Keyword(s):  
Humic Acid ◽  
Response Surface ◽  
Phanerochaete Chrysosporium ◽  
Response Surface Method ◽  
Experimental Results ◽  
White Rot ◽  
White Rot Fungus ◽  
Optimal Conditions ◽  
Surface Method ◽  
Strong Agreement

Abstract The potential hazards of humic acid (HA) associated with hog waste effluent, coupled with increasing awareness of environmental problems, have prompted many countries to control disposal of effluents into water bodies and to maximize removal of HA. Here we employed the white-rot fungus, Phanerochaete chrysosporium, to degrade the HA in composted hog waste effluent, evaluated by the response surface method. Preliminary experiments demonstrate that pH, temperature and quantity of inoculum are significant variables determining success of the fungus. In total, 13 experiments were conducted with three variables designated as A (pH), B (temperature) and C (inoculum amount). The optimal conditions for reduction of HA by P. chrysosporium are pH 6, 31.5°C and an inoculum quantity of 5.86 g. Predicted and experimental results exhibit strong agreement, indicating efficiency in the model obtained by response surface method. Therefore, P. chrysosporium is an effective micro-organism for removal of HA from composted hog waste effluent.

scholarly journals Ekstraksi Oleoresin Lada Hitam Secara Maserasi Menggunakan Metode Permukaan Respon

2018 ◽  
Vol 3 (1) ◽  
Author(s):  
Liya Fitriyana ◽  
Irmayanti Irmayanti ◽  
Putri Meutia Sari ◽  
Virna Muhardina
Keyword(s):  
Response Surface ◽  
Response Surface Method ◽  
Black Pepper ◽  
Extraction Time ◽  
Optimum Conditions ◽  
Iso Standards ◽  
Ultrasonic Methods ◽  
Surface Method ◽  
Ft Ir ◽  
Extraction Solvents

Purpose of this study is to determine the optimum conditions of extraction using conventional methods (maceration) and ultrasonic methods by using response surface method (RSM). The variables used in this study is the ratio of solvent with black pepper, temperature and time of extraction. Solvents used in this study is 99% ethanol. The bigest yield of oleoresin using maceration method was  4.42% with a ratio of solvent and black pepper for 1: 4 g / ml and extraction time of 4.68 hours. GC-MS test showed that the piperine contained in oleoresin extracted by using maceration are respectively<br />69.92% FT-IR test showed that oleoresin extracted using maceration have the same bound that same as piperine bound. Organoleptic test showed that oleoresin produced by using maceration meet the requirement of ISO standards 06-2388-1998 (dark brown color, pepper smell and thick paste form).<br /><br />

scholarly journals OPTIMASI PRODUKSI SENYAWA VANILIN DARI BIOKONVERSI LIGNOSELULOSA TANDAN KOSONG KELAPA SAWIT DENGAN PHANEROCHAETE CHRYSOSPORIUM MENGGUNAKAN RESPONSE SURFACE METHOD

2021 ◽  
Vol 22 (3) ◽  
pp. 201-210
Author(s):  
Rohmah Rohmah ◽  
Sri Suhartini ◽  
Irnia Nurika
Keyword(s):  
Response Surface ◽  
Phanerochaete Chrysosporium ◽  
Response Surface Method ◽  
Design Expert ◽  
Surface Method

Limbah tandan kosong kelapa sawit (TKKS) merupakan limbah hasil produksi minyak sawit yang memiliki kandungan lignoselulosa (lignin, selulosa, hemiselulosa) tinggi, dapat dimanfaatkan untuk produksi senyawa aromatik vanilin dengan menggunakan jamur Phanerochaete chrysosporium dan pelarut etil asetat. Diketahui pelarut etil asetat dan lama ekstraksi berpengaruh terhadap kadar vanilin dan yield vanilin dengan Response Surface Methode (RSM) terdiri dari 4 faktor yang digunakan untuk mengukur sejauh mana faktor-faktor tersebut saling berpengaruh terhadap kadar vanilin. Regresi terdiri dari 13 titik faktorial dengan 6 titik aksial, dan 7 titik tengah melalui softwere Design Expert 7.0.0. Faktor-faktor optimasi digabung untuk mendapatkan nilai optimum untuk menghasilkan kadar dan yield vanilin yang bagus. Hasil penelitian optimasi RSM volume pelarut etil asetat yaitu 100,36 mL dan lama ekstraksi yaitu 120,27 menit yang digunakan dalam penelitian TKKS dengan nilai validasi optimal yang didapatkan yaitu kadar vanilin mendekati hasil prediksi sebesar 0,015% dan yield vanilin sebesar 11,862 µg/g. Hasil dalam penelitian ini menunjukkan penggunaan bio-pretreatment Phanaerocaete chrysosporium sebagai biokonversi TKKS menghasilkan vanilin dapat menekan biaya besar dan meningkatkan nilai fungsi dari limbah TKKS

scholarly journals Optimasi produksi enzim ligninolitik dari medium limbah produksi Pleurotus ostreatus menggunakan metode respons permukaan (Optimization of ligninolytic enzyme production from Pleurotus ostreatus medium waste production using surface response methodology

2018 ◽  
Vol 86 (1) ◽  
Author(s):  
Urip PERWITASARI ◽  
Firda DIMAWARNITA ◽  
Shanti RATNAKOMALA
Keyword(s):  
Response Surface ◽  
Response Surface Method ◽  
Peroxidase Activity ◽  
Pleurotus Ostreatus ◽  
Ligninolytic Enzymes ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
Human Consumption ◽  
Design Expert ◽  
Surface Method

White rot fungi Pleurotus ostreatus has long been produced on a large scale for human consumption. This fungi is known to produce ligninolytic enzymes. The aim of this study was to utilize waste fungal medium from empty fruit bunch oil palm (EFBOP) for production of ligninolytic enzymes. Determination of the optimal conditions in this study used the design of statistical experiments Response Surface Method (RSM) with Design Expert® 10 software. Variables used in this research were EFBOP composition (0, 25, 50, 75, 100 %), part baglog (top, middle, bottom), and time of incubation (1, 2, and 3 month). The highest lignin peroxidase activity was 1.72 U/mL obtained on baglog composition with 50% EFBOP the top past of baglog after 2 months incubation. The highest manganese peroxidase activity was 23.00 U/mL obtained on baglog composition with 100% EFBOP at the bottom of baglog after 3 months incubation and the highest laccase activity was 0.14 U/mL on baglog composition with 100% EFBOP the top past of baglog after 1 month.[Keywords: Pleurotus ostreatus, ligninolytic enzyme, fungal medium waste, response surface methodology]. AbstrakJamur pelapuk putih Pleurotus ostreatus telah lama diproduksi skala besar untuk dikonsumsi. Jamur ini diketahui mampu menghasilkan enzim ligninolitik. Selama ini medium limbah produksi P. ostreatus belum dimanfaatkan. Penelitian ini bertujuan untuk memanfaatkan medium limbah produksi jamur tiram yang berbahan dasar tandan kosong kelapa sawit (TKKS) untuk produksi enzim ligninolitik. Penentuan kondisi optimal pada penelitian ini menggunakan desain eksperimen statistika Metode Respons Permukaan (Response Surface Method (RSM)) dengan software Design Expert® 10. Variabel yang digunakan dalam riset ini adalah konsentrasi TKKS (0, 25, 50, 75, 100 %), bagian baglog (atas, tengah, dan bawah), dan waktu inkubasi (1, 2, dan 3 bulan). Aktivitas lignin peroksidase tertinggi diperoleh pada medium dengan komposisi 50% medium pada bagian atas baglog setelah 2 bulan inkubasi dengan aktivitas sebesar 1,72 U/mL. Aktivitas mangan peroksidase tertinggi diperoleh pada medium komposisi 100% TKKS pada bagian bawah baglog setelah 3 bulan inkubasi sebesar 23,00 U/mL, dan lakase tertinggi pada medium komposisi 100% TKKS pada bagian atas baglog setelah 1 bulan inkubasi, yaitu sebesar 0,14 U/mL.[Kata kunci: Pleurotus ostreatus, enzim ligninolitik, limbah media jamur, Metode Respons Permukaan]

A Proposal of PSO with Presumption Function of the Solution Landscape by Response Surface Method

2014 ◽  
Vol 134 (9) ◽  
pp. 1293-1298
Author(s):  
Toshiya Kaihara ◽  
Nobutada Fuji ◽  
Tomomi Nonaka ◽  
Yuma Tomoi
Keyword(s):  
Response Surface ◽  
Response Surface Method ◽  
Surface Method
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