scholarly journals Polyvinyl alcohol magnetic microspheres containing 2-aminopyrimidin-bis(ethene)bis(dialkylphenylamine) compounds as fluorescent pH indicators

2018 ◽  
Vol 62 (5) ◽  
pp. 563-568 ◽  
Author(s):  
K. V. Laznev ◽  
Zh. V. Ignatovich ◽  
I. N. Kukhta ◽  
A. V. Agabekov
Keyword(s):  
Polyvinyl Alcohol ◽  
Fluorescence Spectra ◽  
Storage Time ◽  
Fluorescent Dyes ◽  
Ph Measurement ◽  
Magnetic Microspheres ◽  
Ph Indicators ◽  
Local Ph

The fluorescent dyes 4,4′-(1E,1′Е)-2,2′-(2-aminopyrimidin-4,6-diyl) bis(ethene-2,l-diyl) bis(N,N-dimethylphenylamine) (АРМ) and 4′-(1E,1′Е)-2,2′-(2-aminopyrimidin-4,6-diyl) bis(ethene-2,l-diyl) bis(N,N-diethylphenylamine) (APE) were incorporated into glutaraldehyde-crosslinked polyvinyl alcohol microspheres containing magnetite. Fluorescence spectra of the obtained microspheres depend on pH of the medium. A technique for pH measurement within the range of 4–9 based on calculation of the green/red ratio of the fragments of color microimages of the microspheres was developed. only negligible loss of the fluorochromes from the microspheres occured during the storage time of 180 days. Thus the microspheres may be used as local pH indicators, e. g. for phagocytosis studies.

Local pH measurement at wet mineral-bacteria/air interface

2014 ◽  
Vol 44 ◽  
pp. 1-3 ◽  
Author(s):  
Djihad Oulkadi ◽  
Sylvie Banon ◽  
Christian Mustin ◽  
Mathieu Etienne
Keyword(s):  
Ph Measurement ◽  
Air Interface ◽  
Local Ph

pH Micro-Sensor from Modified Pt SECM Microelectrode for Local pH Measurement While Chromium Electrodeposition

2020 ◽  
Vol MA2020-01 (28) ◽  
pp. 2106-2106
Author(s):  
Ariane Dasque ◽  
Marie Gressier ◽  
Pierre-Louis Taberna ◽  
Marie-Joelle Menu
Keyword(s):  
Ph Measurement ◽  
Micro Sensor ◽  
Local Ph

Absorption and Fluorescence Properties of p-Substituted Stilbenes in Poly (vinyl chloride) Film

1995 ◽  
Vol 50 (12) ◽  
pp. 1175-1178
Author(s):  
A. Kawski ◽  
B. Kukliński ◽  
T. Nowosielski
Keyword(s):  
Double Bond ◽  
Polyvinyl Alcohol ◽  
Fluorescence Spectra ◽  
Experimental Studies ◽  
Absorption And Fluorescence Spectra ◽  
Emission Anisotropy ◽  
Poly Vinyl Chloride ◽  
Pvc Film ◽  
Linear Molecules ◽  
Chloride Film

Abstract Experimental studies have demonstrated that the absorption and fluorescence spectra of linear molecules with one double bond, i.e. 4-amino-4'-nitrostilbene (ANS), 4-dimethylamino-4'-nitro-stilbene (DNS) and 4,4'-diphenylstilbene (DPS), contained in preheated to 393 K or nonheated polyvinyl chloride) (PVC) films are identical in shape and position, unlike those observed for ANS, DNS and DPS in polyvinyl alcohol) (PVA) films. For polar ANS and DNS molecules in PVC film a decrease in the emission anisotropy with growing wavelength of the fluorescence band was observed. This unusual behaviour of the emission anisotropy is accounted for by the overlap of two close emission bands from different luminescent centres, resulting from different microenvironments of the ANS and DNS molecules in PVC film. The emission anisotropy of nonpolar DPS molecule in PVC does not change within the fluorescence spectrum.

scholarly journals Ultrabright Fluorescent Silica Nanoparticles for Multiplexed Detection

Nanomaterials ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 905
Author(s):  
Saquib Ahmed M. A. Peerzade ◽  
Nadezda Makarova ◽  
Igor Sokolov
Keyword(s):  
Decomposition Method ◽  
Fluorescence Spectra ◽  
Fluorescent Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Silica Matrix ◽  
Dye Molecules ◽  
Multiplexed Detection ◽  
Physical Mixing ◽  
Fluorescent Tagging

Fluorescent tagging is a popular method in biomedical research. Using multiple taggants of different but resolvable fluorescent spectra simultaneously (multiplexing), it is possible to obtain more comprehensive and faster information about various biochemical reactions and diseases, for example, in the method of flow cytometry. Here we report on a first demonstration of the synthesis of ultrabright fluorescent silica nanoporous nanoparticles (Star-dots), which have a large number of complex fluorescence spectra suitable for multiplexed applications. The spectra are obtained via simple physical mixing of different commercially available fluorescent dyes in a synthesizing bath. The resulting particles contain dye molecules encapsulated inside of cylindrical nanochannels of the silica matrix. The distance between the dye molecules is sufficiently small to attain Forster resonance energy transfer (FRET) coupling within a portion of the encapsulated dye molecules. As a result, one can have particles of multiple spectra that can be excited with just one wavelength. We show this for the mixing of five, three, and two dyes. Furthermore, the dyes can be mixed inside of particles in different proportions. This brings another dimension in the complexity of the obtained spectra and makes the number of different resolvable spectra practically unlimited. We demonstrate that the spectra obtained by different mixing of just two dyes inside of each particle can be easily distinguished by using a linear decomposition method. As a practical example, the errors of demultiplexing are measured when sets of a hundred particles are used for tagging.

scholarly journals Pd—PdO pH microprobe for local pH measurement

1989 ◽  
Vol 34 (1) ◽  
pp. 131-136 ◽  
Author(s):  
J. Y. Kim ◽  
Y. H. Lee
Keyword(s):  
Ph Measurement ◽  
Local Ph

scholarly journals Automatic cell identification and enrichment in lung cancer. II. Acridine orange for cell sorting of sputum.

1979 ◽  
Vol 27 (1) ◽  
pp. 552-556 ◽  
Author(s):  
H W Tyrer ◽  
J F Golden ◽  
M H Vansickel ◽  
C K Echols ◽  
J K Frost ◽  
...  
Keyword(s):  
Acridine Orange ◽  
Propidium Iodide ◽  
Cell Sorting ◽  
Fluorescence Spectra ◽  
Fluorescent Dyes ◽  
Appreciable Amount ◽  
Pleural Effusions ◽  
Green Fluorescence ◽  
Total Fluorescence ◽  
Cytoplasmic Differentiation

Fluorescence spectra were obtained from cells from sputum and pleural effusions stained with different fluorescent dyes and fixed by alternate methods. The spectra were referenced to a standard allowing for fluorescence comparisons of unstained and stained cells under various conditions. The metachromasia of acridine orange-stained cells offers nuclear/cytoplasmic differentiation in a single stain; mithramycin and propidium iodide do not. Unstained cells have an appreciable amount of green (546 nm) fluorescence, as does Carbowax in Saccomanno's preservative. Cytoplasm stained with acidine orange also has appreciable green fluorescence. Consequently, cells with much cytoplasm have high total fluorescence. Cytoplasmic fluorescence is negligible with mithramycin or propidium iodide. The metachromasia of acridine orange-stained cells is altered by alcohol and Carbowax levels in fixatives, keeping other factors constant.

Investigation of the Fluorescence Spectra of the Fluorescent Dyes

2014 ◽  
Vol 936 ◽  
pp. 2007-2010
Author(s):  
Saksit Sukprasong ◽  
Tanabat Promjun ◽  
Komsanti Chokethawai ◽  
Athipong Ngamjarurojana
Keyword(s):  
Photon Energy ◽  
Fluorescence Spectra ◽  
Fluorescent Dyes ◽  
Laser Excitation ◽  
Laser Induced Fluorescence ◽  
Luminescence Decay ◽  
Blue Laser ◽  
Excitation Source ◽  
Luminescence Spectra ◽  
Fluorescence Dye

The experiment was conducted to study the effects of concentration, temperature and solvent on the luminescence decay curves obtained in laser-induced fluorescence (LIF) experiment from fluorescent orange dye. Sample solutions of fluorescence dye were prepared by mixing with ethanol solvents at different concentration. The sample was illuminated with a radiation of 470 nm using a blue laser excitation source. Luminescence spectra were recorded using a compact fiber coupled CCD spectrometer and the results were analyzed.The results showed that both concentration and temperature affected the luminescence decay of orange dye in the same way. The luminescence decay was decreased when the concentration and temperature were increased.The appearance of peak positions was slighly shifted to higher wavelength (lower photon energy) when concentration were varied. Then the results of concentration effect were analyzed using peak fit, the appearance of peak positions was slightly shifted from 601.51 to 604.12, 607.64, and 618.44 nm at the concentration of 50, 60, 70 and 80 % by volume, respectively.

pH Micro-Sensor from IrOx SECM Microelectrode for Local pH Measurement While Chromium Electrodeposition

2021 ◽  
Vol MA2021-01 (56) ◽  
pp. 1481-1481
Author(s):  
Ariane Dasque ◽  
Marie Gressier ◽  
Pierre-Louis Taberna ◽  
Marie-Joelle Menu
Keyword(s):  
Ph Measurement ◽  
Micro Sensor ◽  
Local Ph

Fluorescence in Candies Caused by Methyl Anthranilate, a Flavoring Agent

1979 ◽  
Vol 42 (8) ◽  
pp. 658-659 ◽  
Author(s):  
MASATAKE TOYODA ◽  
YOSIDO ITO ◽  
MASAHIRO IWAIDA
Keyword(s):  
Fluorescence Spectra ◽  
Fluorescent Dyes ◽  
Methyl Anthranilate ◽  
Fluorescent Substance ◽  
Addition Level ◽  
Flavoring Agent

A fluorescent substance was found in imported fruit candy. In the elution test of the fluorescent substance, it reacted in the same manner as fluorescent dyes. Further pursuit revealed that the fluorescence was derived from the grape flavor preparation added during manufacture and the fluorescence spectra of the candy and the grape flavor were quite coincident with that of methyl anthranilate. Methyl anthranilate contents in the candy and grape flavor were determined gas chromatographically to be 40.0 and 33,000 ppm, respectively, and it was calculated that the addition level of grape flavor to the candy was about 0.12%.

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